Restriction map for
pF1KE2610
(Linear) (Six-base) MAPSORT of: KIEE2610.seq Check: 6223 from: 1 to: 846
KIEE2610.orf
With 182 enzymes: SgfI *
February 29, 2016 12:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 846
Size: 817 29
AlwNI CAG_nnn'CTG
Cuts at: 0 735 846
Size: 735 111
ApaI G_GGCC'C
Cuts at: 0 237 659 846
Size: 237 422 187
Fragments arranged by size:
422 237 187
BamHI G'GATC_C
Cuts at: 0 560 846
Size: 560 286
BanI G'GyrC_C
Cuts at: 0 751 846
Size: 751 95
BanII G_rGCy'C
Cuts at: 0 130 237 659 672 794 846
Size: 130 107 422 13 122 52
Fragments arranged by size:
422 130 122 107 52 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 468 636 846
Size: 468 168 210
Fragments arranged by size:
468 210 168
BglI GCCn_nnn'nGGC
Cuts at: 0 232 310 676 846
Size: 232 78 366 170
Fragments arranged by size:
366 232 170 78
Bme1580I G_kGCm'C
Cuts at: 0 237 659 846
Size: 237 422 187
Fragments arranged by size:
422 237 187
BmgBI CAC'GTC
Cuts at: 0 25 846
Size: 25 821
BmrI ACTGGGnnnn_n'
Cuts at: 0 553 648 846
Size: 553 95 198
Fragments arranged by size:
553 198 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 376 846
Size: 376 470
BsaBI GATnn'nnATC
Cuts at: 0 175 529 846
Size: 175 354 317
Fragments arranged by size:
354 317 175
BsaWI w'CCGG_w
Cuts at: 0 404 846
Size: 404 442
BseYI C'CCAG_C
Cuts at: 0 29 317 809 846
Size: 29 288 492 37
Fragments arranged by size:
492 288 37 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 229 846
Size: 69 160 617
Fragments arranged by size:
617 160 69
BsiHKAI G_wGCw'C
Cuts at: 0 470 846
Size: 470 376
Bsp1286I G_dGCh'C
Cuts at: 0 130 237 470 659 672 794 846
Size: 130 107 233 189 13 122 52
Fragments arranged by size:
233 189 130 122 107 52 13
BspEI T'CCGG_A
Cuts at: 0 404 846
Size: 404 442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 846
Size: 201 645
BsrBI CCG'CTC
Cuts at: 0 596 846
Size: 596 250
BstAPI GCAn_nnn'nTGC
Cuts at: 0 465 846
Size: 465 381
BstEII G'GTnAC_C
Cuts at: 0 192 213 846
Size: 192 21 633
Fragments arranged by size:
633 192 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 42 556 571 723 846
Size: 42 514 15 152 123
Fragments arranged by size:
514 152 123 42 15
BstYI r'GATC_y
Cuts at: 0 530 560 846
Size: 530 30 286
Fragments arranged by size:
530 286 30
BtgI C'CryG_G
Cuts at: 0 98 200 332 827 846
Size: 98 102 132 495 19
Fragments arranged by size:
495 132 102 98 19
BtsI GCAGTG_nn'
Cuts at: 0 14 443 846
Size: 14 429 403
Fragments arranged by size:
429 403 14
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 507 542 846
Size: 507 35 304
Fragments arranged by size:
507 304 35
EaeI y'GGCC_r
Cuts at: 0 95 423 515 846
Size: 95 328 92 331
Fragments arranged by size:
331 328 95 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 802 846
Size: 802 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 846
Size: 817 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 799 846
Size: 799 47
EcoO109I rG'GnC_Cy
Cuts at: 0 233 655 656 846
Size: 233 422 1 190
Fragments arranged by size:
422 233 190 1
HaeII r_GCGC'y
Cuts at: 0 79 846
Size: 79 767
MscI TGG'CCA
Cuts at: 0 97 425 846
Size: 97 328 421
Fragments arranged by size:
421 328 97
MslI CAynn'nnrTG
Cuts at: 0 199 205 846
Size: 199 6 641
Fragments arranged by size:
641 199 6
MspA1I CmG'CkG
Cuts at: 0 230 321 462 732 846
Size: 230 91 141 270 114
Fragments arranged by size:
270 230 141 114 91
NcoI C'CATG_G
Cuts at: 0 200 846
Size: 200 646
NspI r_CATG'y
Cuts at: 0 42 846
Size: 42 804
PflMI CCAn_nnn'nTGG
Cuts at: 0 324 432 846
Size: 324 108 414
Fragments arranged by size:
414 324 108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 400 432 846
Size: 400 32 414
Fragments arranged by size:
414 400 32
PshAI GACnn'nnGTC
Cuts at: 0 494 846
Size: 494 352
PspOMI G'GGCC_C
Cuts at: 0 233 655 846
Size: 233 422 191
Fragments arranged by size:
422 233 191
PstI C_TGCA'G
Cuts at: 0 461 846
Size: 461 385
PvuII CAG'CTG
Cuts at: 0 321 462 846
Size: 321 141 384
Fragments arranged by size:
384 321 141
SexAI A'CCwGG_T
Cuts at: 0 188 846
Size: 188 658
SfcI C'TryA_G
Cuts at: 0 166 457 846
Size: 166 291 389
Fragments arranged by size:
389 291 166
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 232 846
Size: 232 614
StyI C'CwwG_G
Cuts at: 0 200 387 846
Size: 200 187 459
Fragments arranged by size:
459 200 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 639 846
Size: 639 207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 138 170 846
Size: 138 32 676
Fragments arranged by size:
676 138 32
Enzymes that do cut:
AcuI AlwNI ApaI BamHI BanI BanII BbsI BglI
Bme1580I BmgBI BmrI BsaI BsaBI BsaWI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BstAPI BstEII BstXI
BstYI BtgI BtsI CspCI EaeI EciI Eco57MI EcoNI
EcoO109I HaeII MscI MslI MspA1I NcoI NspI PflMI
PpiI PshAI PspOMI PstI PvuII SexAI SfcI SfiI
StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BsiEI BsiWI
BsmI BsmBI BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI
EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI