Restriction map for
pF1KE2617
(Linear) (Six-base) MAPSORT of: KIEE2617.seq Check: 7323 from: 1 to: 6558
KIEE2617.orf
With 182 enzymes: SgfI *
May 17, 2016 15:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4272 4957 6558
Size: 4272 685 1601
Fragments arranged by size:
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AatII G_ACGT'C
Cuts at: 0 3953 5638 6558
Size: 3953 1685 920
Fragments arranged by size:
3953 1685 920
AccI GT'mk_AC
Cuts at: 0 6507 6542 6558
Size: 6507 35 16
Fragments arranged by size:
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AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 1738 2631 2766 2869 3883 4356 4682
Size: 346 1392 893 135 103 1014 473 326
Cuts at: 4682 5214 5237 5875 6558
Size: 532 23 638 683
Fragments arranged by size:
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326 135 103 23
AfeI AGC'GCT
Cuts at: 0 201 6558
Size: 201 6357
AflII C'TTAA_G
Cuts at: 0 2599 2908 6558
Size: 2599 309 3650
Fragments arranged by size:
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AhdI GACnn_n'nnGTC
Cuts at: 0 2439 5364 6558
Size: 2439 2925 1194
Fragments arranged by size:
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AleI CACnn'nnGTG
Cuts at: 0 6236 6558
Size: 6236 322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1867 4957 6558
Size: 1867 3090 1601
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 1269 2106 4221 6330 6558
Size: 1269 837 2115 2109 228
Fragments arranged by size:
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ApaI G_GGCC'C
Cuts at: 0 3460 5531 6216 6558
Size: 3460 2071 685 342
Fragments arranged by size:
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ApoI r'AATT_y
Cuts at: 0 774 2124 6558
Size: 774 1350 4434
Fragments arranged by size:
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AvaI C'yCGr_G
Cuts at: 0 135 302 1250 5406 5597 6558
Size: 135 167 948 4156 191 961
Fragments arranged by size:
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AvrII C'CTAG_G
Cuts at: 0 2856 5989 6558
Size: 2856 3133 569
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 1517 4956 5588 6195 6397 6418 6558
Size: 1517 3439 632 607 202 21 140
Fragments arranged by size:
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BanII G_rGCy'C
Cuts at: 0 67 309 866 2506 3460 4454 5531 5696
Size: 67 242 557 1640 954 994 1077 165
Cuts at: 5696 5953 6216 6412 6527 6558
Size: 257 263 196 115 31
Fragments arranged by size:
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196 165 115 67 31
BbeI G_GCGC'C
Cuts at: 0 1521 6401 6422 6558
Size: 1521 4880 21 136
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 1340 1566 2868 3455 4494 5899 6558
Size: 1340 226 1302 587 1039 1405 659
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 706 1873 2756 5168 6388 6558
Size: 706 1167 883 2412 1220 170
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 4541 6558
Size: 4541 2017
BfrBI ATG'CAT
Cuts at: 0 3988 6558
Size: 3988 2570
BglI GCCn_nnn'nGGC
Cuts at: 0 100 181 983 1477 1650 1690 3376 3643
Size: 100 81 802 494 173 40 1686 267
Cuts at: 3643 5063 5252 5489 6416 6558
Size: 1420 189 237 927 142
Fragments arranged by size:
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173 142 100 81 40
BglII A'GATC_T
Cuts at: 0 3044 3482 4916 5157 6558
Size: 3044 438 1434 241 1401
Fragments arranged by size:
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BlpI GC'TnA_GC
Cuts at: 0 185 1210 2734 4162 5575 6558
Size: 185 1025 1524 1428 1413 983
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 2618 3460 4440 4747 4961 5531 6200 6216
Size: 2618 842 980 307 214 570 669 16
Cuts at: 6216 6558
Size: 342
Fragments arranged by size:
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16
BmgBI CAC'GTC
Cuts at: 0 1101 5262 6558
Size: 1101 4161 1296
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 1119 1849 2073 6289 6558
Size: 1119 730 224 4216 269
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 328 4119 5736 6558
Size: 328 3791 1617 822
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1460 1822 2461 2656 5174 5944 6511
Size: 745 715 362 639 195 2518 770 567
Cuts at: 6511 6558
Size: 47
Fragments arranged by size:
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Bpu10I CC'TnA_GC
Cuts at: 0 119 420 706 1873 2756 5168 5947 6388
Size: 119 301 286 1167 883 2412 779 441
Cuts at: 6388 6558
Size: 170
Fragments arranged by size:
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119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1929 3625 6085 6278 6558
Size: 1929 1696 2460 193 280
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 1402 1798 1813 4779 5641 6127 6558
Size: 1402 396 15 2966 862 486 431
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 3284 3702 6558
Size: 3284 418 2856
Fragments arranged by size:
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BsaBI GATnn'nnATC
Cuts at: 0 3487 3535 6558
Size: 3487 48 3023
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 1518 3950 4004 5635 6338 6398 6419 6558
Size: 1518 2432 54 1631 703 60 21 139
Fragments arranged by size:
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BsaWI w'CCGG_w
Cuts at: 0 338 745 1484 3137 4181 4325 5027 6558
Size: 338 407 739 1653 1044 144 702 1531
Fragments arranged by size:
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BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1474 1504 1888 1918 3571 3601 5188 5218
Size: 1474 30 384 30 1653 30 1587 30
Cuts at: 5218 5226 5256 5551 5581 5620 5650 5738 5768
Size: 8 30 295 30 39 30 88 30
Cuts at: 5768 6106 6136 6558
Size: 338 30 422
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39 30 30 30 30 30 30 30
30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 1387 1960 2070 2077 2317 2326 2416
Size: 538 849 573 110 7 240 9 90
Cuts at: 2416 2557 2560 4357 4693 4906 5185 5188 5644
Size: 141 3 1797 336 213 279 3 456
Cuts at: 5644 5776 5947 6373 6532 6535 6558
Size: 132 171 426 159 3 23
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23 9 7 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1004 3329 4056 4453 5497 5695 6199 6208
Size: 1004 2325 727 397 1044 198 504 9
Cuts at: 6208 6558
Size: 350
Fragments arranged by size:
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BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 5311 5575 6558
Size: 790 4521 264 983
Fragments arranged by size:
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BsiEI CG_ry'CG
Cuts at: 0 5286 6377 6511 6558
Size: 5286 1091 134 47
Fragments arranged by size:
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BsiHKAI G_wGCw'C
Cuts at: 0 125 866 1634 1856 2062 6527 6558
Size: 125 741 768 222 206 4465 31
Fragments arranged by size:
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BsmI GAATG_Cn'
Cuts at: 0 96 3268 6558
Size: 96 3172 3290
Fragments arranged by size:
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BsmBI CGTCTCn'nnnn_
Cuts at: 0 3396 5626 5642 6189 6558
Size: 3396 2230 16 547 369
Fragments arranged by size:
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Bsp1286I G_dGCh'C
Cuts at: 0 67 125 309 866 1634 1856 2062 2506
Size: 67 58 184 557 768 222 206 444
Cuts at: 2506 2618 3460 4440 4454 4747 4961 5531 5696
Size: 112 842 980 14 293 214 570 165
Cuts at: 5696 5953 6200 6216 6412 6527 6558
Size: 257 247 16 196 115 31
Fragments arranged by size:
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247 222 214 206 196 184 165 115
112 67 58 31 16 14
BspEI T'CCGG_A
Cuts at: 0 338 745 3137 4181 5027 6558
Size: 338 407 2392 1044 846 1531
Fragments arranged by size:
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BspHI T'CATG_A
Cuts at: 0 3527 6558
Size: 3527 3031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 898 2987 4272 4957 6558
Size: 898 2089 1285 685 1601
Fragments arranged by size:
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BsrBI CCG'CTC
Cuts at: 0 3428 5810 6558
Size: 3428 2382 748
Fragments arranged by size:
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BsrDI GCAATG_nn'
Cuts at: 0 213 1619 2249 2868 3869 6558
Size: 213 1406 630 619 1001 2689
Fragments arranged by size:
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BsrFI r'CCGG_y
Cuts at: 0 1520 2379 5239 5425 5488 6558
Size: 1520 859 2860 186 63 1070
Fragments arranged by size:
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BsrGI T'GTAC_A
Cuts at: 0 23 1724 3224 6558
Size: 23 1701 1500 3334
Fragments arranged by size:
3334 1701 1500 23
BssHII G'CGCG_C
Cuts at: 0 6495 6497 6558
Size: 6495 2 61
Fragments arranged by size:
6495 61 2
BssSI C'ACGA_G
Cuts at: 0 1890 5377 6558
Size: 1890 3487 1181
Fragments arranged by size:
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BstAPI GCAn_nnn'nTGC
Cuts at: 0 992 6558
Size: 992 5566
BstBI TT'CG_AA
Cuts at: 0 3401 6558
Size: 3401 3157
BstEII G'GTnAC_C
Cuts at: 0 4695 4980 6315 6558
Size: 4695 285 1335 243
Fragments arranged by size:
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BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 1815 2256 3765 6249 6558
Size: 414 1401 441 1509 2484 309
Fragments arranged by size:
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BstYI r'GATC_y
Cuts at: 0 1145 1296 1487 2102 2852 3044 3482 4916
Size: 1145 151 191 615 750 192 438 1434
Cuts at: 4916 5135 5157 6297 6558
Size: 219 22 1140 261
Fragments arranged by size:
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192 191 151 22
Bsu36I CC'TnA_GG
Cuts at: 0 3076 4852 6558
Size: 3076 1776 1706
Fragments arranged by size:
3076 1776 1706
BtgI C'CryG_G
Cuts at: 0 284 791 963 1082 3848 4167 5523 5615
Size: 284 507 172 119 2766 319 1356 92
Cuts at: 5615 6231 6558
Size: 616 327
Fragments arranged by size:
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119 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 795 1935 3501 6558
Size: 795 1140 1566 3057
Fragments arranged by size:
3057 1566 1140 795
BtsI GCAGTG_nn'
Cuts at: 0 979 2390 2618 5113 5694 5942 6114 6243
Size: 979 1411 228 2495 581 248 172 129
Cuts at: 6243 6558
Size: 315
Fragments arranged by size:
2495 1411 979 581 315 248 228 172
129
ClaI AT'CG_AT
Cuts at: 0 1340 6558
Size: 1340 5218
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4812 4847 6558
Size: 4812 35 1711
Fragments arranged by size:
4812 1711 35
DraI TTT'AAA
Cuts at: 0 527 4635 6558
Size: 527 4108 1923
Fragments arranged by size:
4108 1923 527
DraIII CAC_nnn'GTG
Cuts at: 0 992 4483 4755 6558
Size: 992 3491 272 1803
Fragments arranged by size:
3491 1803 992 272
DrdI GACnn_nn'nnGTC
Cuts at: 0 1780 4807 6538 6558
Size: 1780 3027 1731 20
Fragments arranged by size:
3027 1780 1731 20
EaeI y'GGCC_r
Cuts at: 0 161 287 2246 2333 3409 3767 3783 5283
Size: 161 126 1959 87 1076 358 16 1500
Cuts at: 5283 6332 6374 6558
Size: 1049 42 184
Fragments arranged by size:
1959 1500 1076 1049 358 184 161 126
87 42 16
EagI C'GGCC_G
Cuts at: 0 5283 6374 6558
Size: 5283 1091 184
Fragments arranged by size:
5283 1091 184
EarI CTCTTCn'nnn_
Cuts at: 0 871 1286 1765 1978 1984 2212 2303 2528
Size: 871 415 479 213 6 228 91 225
Cuts at: 2528 2725 3214 3388 3835 3847 4375 5788 6470
Size: 197 489 174 447 12 528 1413 682
Cuts at: 6470 6558
Size: 88
Fragments arranged by size:
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228 225 213 197 174 91 88 12
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3274 4117 4725 6558
Size: 3274 843 608 1833
Fragments arranged by size:
3274 1833 843 608
EcoICRI GAG'CTC
Cuts at: 0 864 6525 6558
Size: 864 5661 33
Fragments arranged by size:
5661 864 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 745 1460 1738 1822 2461 2631 2656
Size: 346 399 715 278 84 639 170 25
Cuts at: 2656 2766 2869 3883 4356 4682 5174 5214 5237
Size: 110 103 1014 473 326 492 40 23
Cuts at: 5237 5875 5944 6511 6558
Size: 638 69 567 47
Fragments arranged by size:
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346 326 278 170 110 103 84 69
47 40 25 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2781 5014 5076 6558
Size: 2781 2233 62 1482
Fragments arranged by size:
2781 2233 1482 62
EcoO109I rG'GnC_Cy
Cuts at: 0 344 710 1683 2760 3071 3456 3457 4218
Size: 344 366 973 1077 311 385 1 761
Cuts at: 4218 5055 5528 5831 6212 6213 6558
Size: 837 473 303 381 1 345
Fragments arranged by size:
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345 344 311 303 1 1
EcoRI G'AATT_C
Cuts at: 0 774 2124 6558
Size: 774 1350 4434
Fragments arranged by size:
4434 1350 774
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2049 3366 3909 6558
Size: 2049 1317 543 2649
Fragments arranged by size:
2649 2049 1317 543
FspI TGC'GCA
Cuts at: 0 5110 6558
Size: 5110 1448
HaeII r_GCGC'y
Cuts at: 0 203 706 1521 5294 5322 6164 6401 6422
Size: 203 503 815 3773 28 842 237 21
Cuts at: 6422 6558
Size: 136
Fragments arranged by size:
3773 842 815 503 237 203 136 28
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1399 1431 1433 1465 2404 2425 2436 2457
Size: 1399 32 2 32 939 21 11 21
Cuts at: 2457 5132 5164 5188 5220 5350 5356 5382 5388
Size: 2675 32 24 32 130 6 26 6
Cuts at: 5388 5620 5652 6558
Size: 232 32 906
Fragments arranged by size:
2675 1399 939 906 232 130 32 32
32 32 32 26 24 21 21 11
6 6 2
HincII GTy'rAC
Cuts at: 0 1422 3303 3708 6508 6558
Size: 1422 1881 405 2800 50
Fragments arranged by size:
2800 1881 1422 405 50
KasI G'GCGC_C
Cuts at: 0 1517 6397 6418 6558
Size: 1517 4880 21 140
Fragments arranged by size:
4880 1517 140 21
MfeI C'AATT_G
Cuts at: 0 408 6558
Size: 408 6150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 479 494 3342 5163 5456 5606 6558
Size: 77 402 15 2848 1821 293 150 952
Fragments arranged by size:
2848 1821 952 402 293 150 77 15
MscI TGG'CCA
Cuts at: 0 2248 2335 3411 3769 6558
Size: 2248 87 1076 358 2789
Fragments arranged by size:
2789 2248 1076 358 87
MslI CAynn'nnrTG
Cuts at: 0 560 1081 1393 1813 1849 2254 3541 4625
Size: 560 521 312 420 36 405 1287 1084
Cuts at: 4625 4688 4783 5551 5713 6236 6558
Size: 63 95 768 162 523 322
Fragments arranged by size:
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322 312 162 95 63 36
MspA1I CmG'CkG
Cuts at: 0 106 240 3329 4060 5167 5209 5325 6392
Size: 106 134 3089 731 1107 42 116 1067
Cuts at: 6392 6558
Size: 166
Fragments arranged by size:
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42
NaeI GCC'GGC
Cuts at: 0 2381 5490 6558
Size: 2381 3109 1068
Fragments arranged by size:
3109 2381 1068
NarI GG'CG_CC
Cuts at: 0 1518 6398 6419 6558
Size: 1518 4880 21 139
Fragments arranged by size:
4880 1518 139 21
NcoI C'CATG_G
Cuts at: 0 791 1082 5615 6558
Size: 791 291 4533 943
Fragments arranged by size:
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NgoMIV G'CCGG_C
Cuts at: 0 2379 5488 6558
Size: 2379 3109 1070
Fragments arranged by size:
3109 2379 1070
NsiI A_TGCA'T
Cuts at: 0 3990 6558
Size: 3990 2568
NspI r_CATG'y
Cuts at: 0 1398 3112 3828 4422 4527 4788 6558
Size: 1398 1714 716 594 105 261 1770
Fragments arranged by size:
1770 1714 1398 716 594 261 105
PasI CC'CwG_GG
Cuts at: 0 5466 6217 6558
Size: 5466 751 341
Fragments arranged by size:
5466 751 341
PflMI CCAn_nnn'nTGG
Cuts at: 0 4623 6330 6558
Size: 4623 1707 228
Fragments arranged by size:
4623 1707 228
PfoI T'CCnGG_A
Cuts at: 0 1799 2972 5015 6527 6558
Size: 1799 1173 2043 1512 31
Fragments arranged by size:
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PpuMI rG'GwC_Cy
Cuts at: 0 2760 6558
Size: 2760 3798
PsiI TTA'TAA
Cuts at: 0 601 6558
Size: 601 5957
PspOMI G'GGCC_C
Cuts at: 0 3456 5527 6212 6558
Size: 3456 2071 685 346
Fragments arranged by size:
3456 2071 685 346
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3718 3750 6558
Size: 3718 32 2808
Fragments arranged by size:
3718 2808 32
PstI C_TGCA'G
Cuts at: 0 1540 1742 3179 4968 5099 5689 6445 6558
Size: 1540 202 1437 1789 131 590 756 113
Fragments arranged by size:
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PvuII CAG'CTG
Cuts at: 0 3329 4060 6558
Size: 3329 731 2498
Fragments arranged by size:
3329 2498 731
SacI G_AGCT'C
Cuts at: 0 866 6527 6558
Size: 866 5661 31
Fragments arranged by size:
5661 866 31
SalI G'TCGA_C
Cuts at: 0 6506 6558
Size: 6506 52
SapI GCTCTTCn'nnn_
Cuts at: 0 871 3214 3835 6558
Size: 871 2343 621 2723
Fragments arranged by size:
2723 2343 871 621
SbfI CC_TGCA'GG
Cuts at: 0 1742 4968 6558
Size: 1742 3226 1590
Fragments arranged by size:
3226 1742 1590
ScaI AGT'ACT
Cuts at: 0 3065 5023 6558
Size: 3065 1958 1535
Fragments arranged by size:
3065 1958 1535
SexAI A'CCwGG_T
Cuts at: 0 1916 4949 6558
Size: 1916 3033 1609
Fragments arranged by size:
3033 1916 1609
SfcI C'TryA_G
Cuts at: 0 1536 1738 2525 3175 4964 5095 5685 6441
Size: 1536 202 787 650 1789 131 590 756
Cuts at: 6441 6558
Size: 117
Fragments arranged by size:
1789 1536 787 756 650 590 202 131
117
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1477 1690 6558
Size: 1477 213 4868
Fragments arranged by size:
4868 1477 213
SfoI GGC'GCC
Cuts at: 0 1519 6399 6420 6558
Size: 1519 4880 21 138
Fragments arranged by size:
4880 1519 138 21
SgrAI Cr'CCGG_yG
Cuts at: 0 1520 6558
Size: 1520 5038
SmaI CCC'GGG
Cuts at: 0 137 1252 5599 6558
Size: 137 1115 4347 959
Fragments arranged by size:
4347 1115 959 137
SmlI C'TyrA_G
Cuts at: 0 1908 2599 2908 3640 6064 6293 6558
Size: 1908 691 309 732 2424 229 265
Fragments arranged by size:
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SpeI A'CTAG_T
Cuts at: 0 2881 6558
Size: 2881 3677
SphI G_CATG'C
Cuts at: 0 1398 4422 6558
Size: 1398 3024 2136
Fragments arranged by size:
3024 2136 1398
StuI AGG'CCT
Cuts at: 0 1744 4393 5683 5945 5994 6558
Size: 1744 2649 1290 262 49 564
Fragments arranged by size:
2649 1744 1290 564 262 49
StyI C'CwwG_G
Cuts at: 0 45 791 1082 1244 2396 2856 3095 3884
Size: 45 746 291 162 1152 460 239 789
Cuts at: 3884 4608 4667 5615 5989 6558
Size: 724 59 948 374 569
Fragments arranged by size:
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291 239 162 59 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1426 5885 6337 6476 6525 6558
Size: 1426 4459 452 139 49 33
Fragments arranged by size:
4459 1426 452 139 49 33
TatI w'GTAC_w
Cuts at: 0 23 1106 1185 1724 3063 3224 3587 3686
Size: 23 1083 79 539 1339 161 363 99
Cuts at: 3686 5021 6558
Size: 1335 1537
Fragments arranged by size:
1537 1339 1335 1083 539 363 161 99
79 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 680 712 1474 1506 5226 5258
Size: 34 32 614 32 762 32 3720 32
Cuts at: 5258 5551 5583 6032 6064 6106 6138 6558
Size: 293 32 449 32 42 32 420
Fragments arranged by size:
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32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1413 2764 2872 6558
Size: 1413 1351 108 3686
Fragments arranged by size:
3686 1413 1351 108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 855 2463 6249 6327 6360 6558
Size: 471 384 1608 3786 78 33 198
Fragments arranged by size:
3786 1608 471 384 198 78 33
XmaI C'CCGG_G
Cuts at: 0 135 1250 5597 6558
Size: 135 1115 4347 961
Fragments arranged by size:
4347 1115 961 135
XmnI GAAnn'nnTTC
Cuts at: 0 1531 2239 2962 3538 5970 6558
Size: 1531 708 723 576 2432 588
Fragments arranged by size:
2432 1531 723 708 588 576
ZraI GAC'GTC
Cuts at: 0 3951 5636 6558
Size: 3951 1685 922
Fragments arranged by size:
3951 1685 922
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AhdI AleI
AlfI AlwNI ApaI ApoI AvaI AvrII BanI BanII
BbeI BbsI BbvCI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PflMI
PfoI PpuMI PsiI PspOMI PsrI PstI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflIII AgeI AloI ApaLI AscI AseI
AsiSI BaeI BamHI BcgI BciVI BmtI BsiWI BstZ17I
EcoRV FseI FspAI HindIII HpaI KpnI MluI NdeI
NheI NotI NruI PacI PciI PmeI PmlI PpiI
PshAI PspXI PvuI RsrII SacII SanDI SgfI SnaBI
SrfI SspI SwaI XbaI XhoI