Restriction map for
pF1KE2627
(Linear) (Six-base) MAPSORT of: KIEE2627.seq Check: 3361 from: 1 to: 813
KIEE2627.orf
With 182 enzymes: SgfI *
May 31, 2016 13:40 ..
Acc65I G'GTAC_C
Cuts at: 0 802 813
Size: 802 11
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 813
Size: 730 83
AlwNI CAG_nnn'CTG
Cuts at: 0 111 174 282 792 813
Size: 111 63 108 510 21
Fragments arranged by size:
510 111 108 63 21
ApaI G_GGCC'C
Cuts at: 0 195 813
Size: 195 618
ApoI r'AATT_y
Cuts at: 0 589 813
Size: 589 224
AvaI C'yCGr_G
Cuts at: 0 468 813
Size: 468 345
BamHI G'GATC_C
Cuts at: 0 524 813
Size: 524 289
BanI G'GyrC_C
Cuts at: 0 157 472 631 802 813
Size: 157 315 159 171 11
Fragments arranged by size:
315 171 159 157 11
BanII G_rGCy'C
Cuts at: 0 11 195 404 646 813
Size: 11 184 209 242 167
Fragments arranged by size:
242 209 184 167 11
BbeI G_GCGC'C
Cuts at: 0 635 813
Size: 635 178
BbsI GAAGACnn'nnnn_
Cuts at: 0 63 600 813
Size: 63 537 213
Fragments arranged by size:
537 213 63
BglI GCCn_nnn'nGGC
Cuts at: 0 230 813
Size: 230 583
Bme1580I G_kGCm'C
Cuts at: 0 195 475 813
Size: 195 280 338
Fragments arranged by size:
338 280 195
BmgBI CAC'GTC
Cuts at: 0 240 813
Size: 240 573
BmrI ACTGGGnnnn_n'
Cuts at: 0 612 813
Size: 612 201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 795 813
Size: 30 765 18
Fragments arranged by size:
765 30 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 554 739 813
Size: 12 542 185 74
Fragments arranged by size:
542 185 74 12
BsaAI yAC'GTr
Cuts at: 0 267 663 813
Size: 267 396 150
Fragments arranged by size:
396 267 150
BsaHI Gr'CG_yC
Cuts at: 0 632 813
Size: 632 181
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 640 754 813
Size: 52 588 114 59
Fragments arranged by size:
588 114 59 52
BseYI C'CCAG_C
Cuts at: 0 275 282 813
Size: 275 7 531
Fragments arranged by size:
531 275 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 813
Size: 760 53
BsiHKAI G_wGCw'C
Cuts at: 0 11 404 428 813
Size: 11 393 24 385
Fragments arranged by size:
393 385 24 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 246 813
Size: 246 567
Bsp1286I G_dGCh'C
Cuts at: 0 11 195 404 428 475 646 813
Size: 11 184 209 24 47 171 167
Fragments arranged by size:
209 184 171 167 47 24 11
BsrBI CCG'CTC
Cuts at: 0 14 304 560 813
Size: 14 290 256 253
Fragments arranged by size:
290 256 253 14
BsrFI r'CCGG_y
Cuts at: 0 535 813
Size: 535 278
BstAPI GCAn_nnn'nTGC
Cuts at: 0 423 792 813
Size: 423 369 21
Fragments arranged by size:
423 369 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 520 813
Size: 520 293
BstYI r'GATC_y
Cuts at: 0 524 813
Size: 524 289
BtgI C'CryG_G
Cuts at: 0 134 336 456 796 813
Size: 134 202 120 340 17
Fragments arranged by size:
340 202 134 120 17
BtsI GCAGTG_nn'
Cuts at: 0 412 710 813
Size: 412 298 103
Fragments arranged by size:
412 298 103
DrdI GACnn_nn'nnGTC
Cuts at: 0 349 604 813
Size: 349 255 209
Fragments arranged by size:
349 255 209
EaeI y'GGCC_r
Cuts at: 0 53 100 293 381 813
Size: 53 47 193 88 432
Fragments arranged by size:
432 193 88 53 47
EarI CTCTTCn'nnn_
Cuts at: 0 70 409 813
Size: 70 339 404
Fragments arranged by size:
404 339 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 662 813
Size: 662 151
EcoICRI GAG'CTC
Cuts at: 0 9 402 813
Size: 9 393 411
Fragments arranged by size:
411 393 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 554 730 739 813
Size: 12 542 176 9 74
Fragments arranged by size:
542 176 74 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 27 166 813
Size: 27 139 647
Fragments arranged by size:
647 139 27
EcoO109I rG'GnC_Cy
Cuts at: 0 108 191 342 714 813
Size: 108 83 151 372 99
Fragments arranged by size:
372 151 108 99 83
HaeII r_GCGC'y
Cuts at: 0 456 635 699 813
Size: 456 179 64 114
Fragments arranged by size:
456 179 114 64
KasI G'GCGC_C
Cuts at: 0 631 813
Size: 631 182
KpnI G_GTAC'C
Cuts at: 0 806 813
Size: 806 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 813
Size: 671 142
MscI TGG'CCA
Cuts at: 0 55 102 383 813
Size: 55 47 281 430
Fragments arranged by size:
430 281 55 47
MspA1I CmG'CkG
Cuts at: 0 279 338 420 458 574 813
Size: 279 59 82 38 116 239
Fragments arranged by size:
279 239 116 82 59 38
NarI GG'CG_CC
Cuts at: 0 632 813
Size: 632 181
NcoI C'CATG_G
Cuts at: 0 134 813
Size: 134 679
PasI CC'CwG_GG
Cuts at: 0 507 813
Size: 507 306
PflMI CCAn_nnn'nTGG
Cuts at: 0 282 813
Size: 282 531
PpuMI rG'GwC_Cy
Cuts at: 0 342 813
Size: 342 471
PspOMI G'GGCC_C
Cuts at: 0 191 813
Size: 191 622
PstI C_TGCA'G
Cuts at: 0 419 813
Size: 419 394
PvuII CAG'CTG
Cuts at: 0 279 420 574 813
Size: 279 141 154 239
Fragments arranged by size:
279 239 154 141
SacI G_AGCT'C
Cuts at: 0 11 404 813
Size: 11 393 409
Fragments arranged by size:
409 393 11
SacII CC_GC'GG
Cuts at: 0 339 459 813
Size: 339 120 354
Fragments arranged by size:
354 339 120
SapI GCTCTTCn'nnn_
Cuts at: 0 409 813
Size: 409 404
SfcI C'TryA_G
Cuts at: 0 124 415 813
Size: 124 291 398
Fragments arranged by size:
398 291 124
SfoI GGC'GCC
Cuts at: 0 633 813
Size: 633 180
SmaI CCC'GGG
Cuts at: 0 470 813
Size: 470 343
StyI C'CwwG_G
Cuts at: 0 134 519 806 813
Size: 134 385 287 7
Fragments arranged by size:
385 287 134 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 611 643 813
Size: 611 32 170
Fragments arranged by size:
611 170 32
XmaI C'CCGG_G
Cuts at: 0 468 813
Size: 468 345
Enzymes that do cut:
Acc65I AcuI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BplI
BpmI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BsrBI BsrFI BstAPI BstXI BstYI BtgI BtsI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII KasI KpnI MmeI MscI MspA1I NarI NcoI
PasI PflMI PpuMI PspOMI PstI PvuII SacI SacII
SapI SfcI SfoI SmaI StyI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BsiEI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII EagI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI
MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI Tth111I
XbaI XcmI XhoI XmnI ZraI