Restriction map for
pF1KE2633
(Linear) (Six-base) MAPSORT of: KIEE2633.seq Check: 6794 from: 1 to: 2586
KIEE2633.orf
With 182 enzymes: SgfI *
August 3, 2016 15:29 ..
AccI GT'mk_AC
Cuts at: 0 1219 1590 2586
Size: 1219 371 996
Fragments arranged by size:
1219 996 371
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 190 334 506 635 902 967 1055
Size: 25 165 144 172 129 267 65 88
Cuts at: 1055 2125 2299 2467 2586
Size: 1070 174 168 119
Fragments arranged by size:
1070 267 174 172 168 165 144 129
119 88 65 25
AflIII A'CryG_T
Cuts at: 0 1187 2586
Size: 1187 1399
AlwNI CAG_nnn'CTG
Cuts at: 0 283 613 861 1345 1740 2586
Size: 283 330 248 484 395 846
Fragments arranged by size:
846 484 395 330 283 248
ApaI G_GGCC'C
Cuts at: 0 16 2586
Size: 16 2570
ApaLI G'TGCA_C
Cuts at: 0 89 2586
Size: 89 2497
ApoI r'AATT_y
Cuts at: 0 79 112 133 504 1634 2586
Size: 79 33 21 371 1130 952
Fragments arranged by size:
1130 952 371 79 33 21
AseI AT'TA_AT
Cuts at: 0 71 2066 2586
Size: 71 1995 520
Fragments arranged by size:
1995 520 71
AvaI C'yCGr_G
Cuts at: 0 1239 2586
Size: 1239 1347
BamHI G'GATC_C
Cuts at: 0 2501 2586
Size: 2501 85
BanII G_rGCy'C
Cuts at: 0 16 1246 1809 2586
Size: 16 1230 563 777
Fragments arranged by size:
1230 777 563 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 623 1723 1751 2003 2305 2586
Size: 623 1100 28 252 302 281
Fragments arranged by size:
1100 623 302 281 252 28
BglII A'GATC_T
Cuts at: 0 1784 1820 2586
Size: 1784 36 766
Fragments arranged by size:
1784 766 36
Bme1580I G_kGCm'C
Cuts at: 0 16 93 432 2586
Size: 16 77 339 2154
Fragments arranged by size:
2154 339 77 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 1071 2586
Size: 1071 1515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 655 1178 1363 1455 1939 2586
Size: 427 228 523 185 92 484 647
Fragments arranged by size:
647 523 484 427 228 185 92
Bpu10I CC'TnA_GC
Cuts at: 0 1690 2366 2586
Size: 1690 676 220
Fragments arranged by size:
1690 676 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1043 2326 2586
Size: 227 816 1283 260
Fragments arranged by size:
1283 816 260 227
BsaI GGTCTCn'nnnn_
Cuts at: 0 1655 2586
Size: 1655 931
BsaAI yAC'GTr
Cuts at: 0 88 836 2091 2586
Size: 88 748 1255 495
Fragments arranged by size:
1255 748 495 88
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 511 1108 1456 2586
Size: 175 336 597 348 1130
Fragments arranged by size:
1130 597 348 336 175
BseYI C'CCAG_C
Cuts at: 0 23 227 2586
Size: 23 204 2359
Fragments arranged by size:
2359 204 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 1775 2509 2586
Size: 182 1593 734 77
Fragments arranged by size:
1593 734 182 77
BsiHKAI G_wGCw'C
Cuts at: 0 93 389 1215 2586
Size: 93 296 826 1371
Fragments arranged by size:
1371 826 296 93
Bsp1286I G_dGCh'C
Cuts at: 0 16 93 389 432 1215 1246 1809 2586
Size: 16 77 296 43 783 31 563 777
Fragments arranged by size:
783 777 563 296 77 43 31 16
BsrBI CCG'CTC
Cuts at: 0 2499 2586
Size: 2499 87
BsrDI GCAATG_nn'
Cuts at: 0 1246 2586
Size: 1246 1340
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1288 1971 2586
Size: 1288 683 615
Fragments arranged by size:
1288 683 615
BstBI TT'CG_AA
Cuts at: 0 222 2586
Size: 222 2364
BstYI r'GATC_y
Cuts at: 0 165 1161 1784 1820 2135 2501 2586
Size: 165 996 623 36 315 366 85
Fragments arranged by size:
996 623 366 315 165 85 36
Bsu36I CC'TnA_GG
Cuts at: 0 400 2586
Size: 400 2186
BtgI C'CryG_G
Cuts at: 0 1113 2586
Size: 1113 1473
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1447 2586
Size: 1447 1139
BtsI GCAGTG_nn'
Cuts at: 0 2026 2586
Size: 2026 560
DraI TTT'AAA
Cuts at: 0 1194 2156 2586
Size: 1194 962 430
Fragments arranged by size:
1194 962 430
EarI CTCTTCn'nnn_
Cuts at: 0 44 166 1085 1445 1826 1913 2333 2390
Size: 44 122 919 360 381 87 420 57
Cuts at: 2390 2586
Size: 196
Fragments arranged by size:
919 420 381 360 196 122 87 57
44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 190 334 427 506 635 655 902
Size: 25 165 144 93 79 129 20 247
Cuts at: 902 967 1055 1178 1363 1455 1939 2125 2299
Size: 65 88 123 185 92 484 186 174
Cuts at: 2299 2467 2586
Size: 168 119
Fragments arranged by size:
484 247 186 185 174 168 165 144
129 123 119 93 92 88 79 65
25 20
EcoRI G'AATT_C
Cuts at: 0 1634 2586
Size: 1634 952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2182 2586
Size: 2182 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 984 1016 1327 1348 1359 1380 1444 1476
Size: 984 32 311 21 11 21 64 32
Cuts at: 1476 2586
Size: 1110
Fragments arranged by size:
1110 984 311 64 32 32 21 21
11
HincII GTy'rAC
Cuts at: 0 1403 2586
Size: 1403 1183
HindIII A'AGCT_T
Cuts at: 0 1259 2467 2586
Size: 1259 1208 119
Fragments arranged by size:
1259 1208 119
HpaI GTT'AAC
Cuts at: 0 1403 2586
Size: 1403 1183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 954 2252 2586
Size: 954 1298 334
Fragments arranged by size:
1298 954 334
MslI CAynn'nnrTG
Cuts at: 0 2068 2586
Size: 2068 518
MspA1I CmG'CkG
Cuts at: 0 231 1683 2586
Size: 231 1452 903
Fragments arranged by size:
1452 903 231
NspI r_CATG'y
Cuts at: 0 894 1191 1978 2586
Size: 894 297 787 608
Fragments arranged by size:
894 787 608 297
PasI CC'CwG_GG
Cuts at: 0 1482 2586
Size: 1482 1104
PciI A'CATG_T
Cuts at: 0 1187 2586
Size: 1187 1399
PflMI CCAn_nnn'nTGG
Cuts at: 0 795 861 1488 2586
Size: 795 66 627 1098
Fragments arranged by size:
1098 795 627 66
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 2586
Size: 376 32 2178
Fragments arranged by size:
2178 376 32
PspOMI G'GGCC_C
Cuts at: 0 12 2586
Size: 12 2574
PvuII CAG'CTG
Cuts at: 0 231 1683 2586
Size: 231 1452 903
Fragments arranged by size:
1452 903 231
RsrII CG'GwC_CG
Cuts at: 0 554 2586
Size: 554 2032
SapI GCTCTTCn'nnn_
Cuts at: 0 1913 2333 2586
Size: 1913 420 253
Fragments arranged by size:
1913 420 253
SexAI A'CCwGG_T
Cuts at: 0 1664 2586
Size: 1664 922
SfcI C'TryA_G
Cuts at: 0 305 742 1220 1678 2018 2586
Size: 305 437 478 458 340 568
Fragments arranged by size:
568 478 458 437 340 305
SmlI C'TyrA_G
Cuts at: 0 242 1058 2305 2586
Size: 242 816 1247 281
Fragments arranged by size:
1247 816 281 242
SspI AAT'ATT
Cuts at: 0 261 2586
Size: 261 2325
StuI AGG'CCT
Cuts at: 0 640 1813 2586
Size: 640 1173 773
Fragments arranged by size:
1173 773 640
StyI C'CwwG_G
Cuts at: 0 1368 2519 2586
Size: 1368 1151 67
Fragments arranged by size:
1368 1151 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 542 2586
Size: 542 2044
Tth111I GACn'n_nGTC
Cuts at: 0 1458 2586
Size: 1458 1128
XbaI T'CTAG_A
Cuts at: 0 699 2586
Size: 699 1887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1387 2586
Size: 1387 1199
XmnI GAAnn'nnTTC
Cuts at: 0 371 2586
Size: 371 2215
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApaLI ApoI AseI
AvaI BamHI BanII BbsI BglII Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BseRI BseYI BsgI BsiHKAI
Bsp1286I BsrBI BsrDI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI EarI Eco57MI EcoRI FalI Hin4I
HincII HindIII HpaI MmeI MslI MspA1I NspI PasI
PciI PflMI PpiI PspOMI PvuII RsrII SapI SexAI
SfcI SmlI SspI StuI StyI TaqII Tth111I XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AscI AsiSI AvrII BaeI BanI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BmtI BplI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI
BssHII BssSI BstEII BstXI BstZ17I ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV
FseI FspI FspAI HaeII KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI TatI TstI XhoI
XmaI ZraI