(Linear) (Six-base) MAPSORT of: KIEE2633.seq Check: 6794 from: 1 to: 2586 KIEE2633.orf With 182 enzymes: SgfI * August 3, 2016 15:29 .. AccI GT'mk_AC Cuts at: 0 1219 1590 2586 Size: 1219 371 996 Fragments arranged by size: 1219 996 371 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 190 334 506 635 902 967 1055 Size: 25 165 144 172 129 267 65 88 Cuts at: 1055 2125 2299 2467 2586 Size: 1070 174 168 119 Fragments arranged by size: 1070 267 174 172 168 165 144 129 119 88 65 25 AflIII A'CryG_T Cuts at: 0 1187 2586 Size: 1187 1399 AlwNI CAG_nnn'CTG Cuts at: 0 283 613 861 1345 1740 2586 Size: 283 330 248 484 395 846 Fragments arranged by size: 846 484 395 330 283 248 ApaI G_GGCC'C Cuts at: 0 16 2586 Size: 16 2570 ApaLI G'TGCA_C Cuts at: 0 89 2586 Size: 89 2497 ApoI r'AATT_y Cuts at: 0 79 112 133 504 1634 2586 Size: 79 33 21 371 1130 952 Fragments arranged by size: 1130 952 371 79 33 21 AseI AT'TA_AT Cuts at: 0 71 2066 2586 Size: 71 1995 520 Fragments arranged by size: 1995 520 71 AvaI C'yCGr_G Cuts at: 0 1239 2586 Size: 1239 1347 BamHI G'GATC_C Cuts at: 0 2501 2586 Size: 2501 85 BanII G_rGCy'C Cuts at: 0 16 1246 1809 2586 Size: 16 1230 563 777 Fragments arranged by size: 1230 777 563 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 623 1723 1751 2003 2305 2586 Size: 623 1100 28 252 302 281 Fragments arranged by size: 1100 623 302 281 252 28 BglII A'GATC_T Cuts at: 0 1784 1820 2586 Size: 1784 36 766 Fragments arranged by size: 1784 766 36 Bme1580I G_kGCm'C Cuts at: 0 16 93 432 2586 Size: 16 77 339 2154 Fragments arranged by size: 2154 339 77 16 BmrI ACTGGGnnnn_n' Cuts at: 0 1071 2586 Size: 1071 1515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 427 655 1178 1363 1455 1939 2586 Size: 427 228 523 185 92 484 647 Fragments arranged by size: 647 523 484 427 228 185 92 Bpu10I CC'TnA_GC Cuts at: 0 1690 2366 2586 Size: 1690 676 220 Fragments arranged by size: 1690 676 220 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 227 1043 2326 2586 Size: 227 816 1283 260 Fragments arranged by size: 1283 816 260 227 BsaI GGTCTCn'nnnn_ Cuts at: 0 1655 2586 Size: 1655 931 BsaAI yAC'GTr Cuts at: 0 88 836 2091 2586 Size: 88 748 1255 495 Fragments arranged by size: 1255 748 495 88 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 175 511 1108 1456 2586 Size: 175 336 597 348 1130 Fragments arranged by size: 1130 597 348 336 175 BseYI C'CCAG_C Cuts at: 0 23 227 2586 Size: 23 204 2359 Fragments arranged by size: 2359 204 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 182 1775 2509 2586 Size: 182 1593 734 77 Fragments arranged by size: 1593 734 182 77 BsiHKAI G_wGCw'C Cuts at: 0 93 389 1215 2586 Size: 93 296 826 1371 Fragments arranged by size: 1371 826 296 93 Bsp1286I G_dGCh'C Cuts at: 0 16 93 389 432 1215 1246 1809 2586 Size: 16 77 296 43 783 31 563 777 Fragments arranged by size: 783 777 563 296 77 43 31 16 BsrBI CCG'CTC Cuts at: 0 2499 2586 Size: 2499 87 BsrDI GCAATG_nn' Cuts at: 0 1246 2586 Size: 1246 1340 BstAPI GCAn_nnn'nTGC Cuts at: 0 1288 1971 2586 Size: 1288 683 615 Fragments arranged by size: 1288 683 615 BstBI TT'CG_AA Cuts at: 0 222 2586 Size: 222 2364 BstYI r'GATC_y Cuts at: 0 165 1161 1784 1820 2135 2501 2586 Size: 165 996 623 36 315 366 85 Fragments arranged by size: 996 623 366 315 165 85 36 Bsu36I CC'TnA_GG Cuts at: 0 400 2586 Size: 400 2186 BtgI C'CryG_G Cuts at: 0 1113 2586 Size: 1113 1473 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1447 2586 Size: 1447 1139 BtsI GCAGTG_nn' Cuts at: 0 2026 2586 Size: 2026 560 DraI TTT'AAA Cuts at: 0 1194 2156 2586 Size: 1194 962 430 Fragments arranged by size: 1194 962 430 EarI CTCTTCn'nnn_ Cuts at: 0 44 166 1085 1445 1826 1913 2333 2390 Size: 44 122 919 360 381 87 420 57 Cuts at: 2390 2586 Size: 196 Fragments arranged by size: 919 420 381 360 196 122 87 57 44 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 190 334 427 506 635 655 902 Size: 25 165 144 93 79 129 20 247 Cuts at: 902 967 1055 1178 1363 1455 1939 2125 2299 Size: 65 88 123 185 92 484 186 174 Cuts at: 2299 2467 2586 Size: 168 119 Fragments arranged by size: 484 247 186 185 174 168 165 144 129 123 119 93 92 88 79 65 25 20 EcoRI G'AATT_C Cuts at: 0 1634 2586 Size: 1634 952 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2182 2586 Size: 2182 404 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 984 1016 1327 1348 1359 1380 1444 1476 Size: 984 32 311 21 11 21 64 32 Cuts at: 1476 2586 Size: 1110 Fragments arranged by size: 1110 984 311 64 32 32 21 21 11 HincII GTy'rAC Cuts at: 0 1403 2586 Size: 1403 1183 HindIII A'AGCT_T Cuts at: 0 1259 2467 2586 Size: 1259 1208 119 Fragments arranged by size: 1259 1208 119 HpaI GTT'AAC Cuts at: 0 1403 2586 Size: 1403 1183 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 954 2252 2586 Size: 954 1298 334 Fragments arranged by size: 1298 954 334 MslI CAynn'nnrTG Cuts at: 0 2068 2586 Size: 2068 518 MspA1I CmG'CkG Cuts at: 0 231 1683 2586 Size: 231 1452 903 Fragments arranged by size: 1452 903 231 NspI r_CATG'y Cuts at: 0 894 1191 1978 2586 Size: 894 297 787 608 Fragments arranged by size: 894 787 608 297 PasI CC'CwG_GG Cuts at: 0 1482 2586 Size: 1482 1104 PciI A'CATG_T Cuts at: 0 1187 2586 Size: 1187 1399 PflMI CCAn_nnn'nTGG Cuts at: 0 795 861 1488 2586 Size: 795 66 627 1098 Fragments arranged by size: 1098 795 627 66 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 376 408 2586 Size: 376 32 2178 Fragments arranged by size: 2178 376 32 PspOMI G'GGCC_C Cuts at: 0 12 2586 Size: 12 2574 PvuII CAG'CTG Cuts at: 0 231 1683 2586 Size: 231 1452 903 Fragments arranged by size: 1452 903 231 RsrII CG'GwC_CG Cuts at: 0 554 2586 Size: 554 2032 SapI GCTCTTCn'nnn_ Cuts at: 0 1913 2333 2586 Size: 1913 420 253 Fragments arranged by size: 1913 420 253 SexAI A'CCwGG_T Cuts at: 0 1664 2586 Size: 1664 922 SfcI C'TryA_G Cuts at: 0 305 742 1220 1678 2018 2586 Size: 305 437 478 458 340 568 Fragments arranged by size: 568 478 458 437 340 305 SmlI C'TyrA_G Cuts at: 0 242 1058 2305 2586 Size: 242 816 1247 281 Fragments arranged by size: 1247 816 281 242 SspI AAT'ATT Cuts at: 0 261 2586 Size: 261 2325 StuI AGG'CCT Cuts at: 0 640 1813 2586 Size: 640 1173 773 Fragments arranged by size: 1173 773 640 StyI C'CwwG_G Cuts at: 0 1368 2519 2586 Size: 1368 1151 67 Fragments arranged by size: 1368 1151 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 542 2586 Size: 542 2044 Tth111I GACn'n_nGTC Cuts at: 0 1458 2586 Size: 1458 1128 XbaI T'CTAG_A Cuts at: 0 699 2586 Size: 699 1887 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1387 2586 Size: 1387 1199 XmnI GAAnn'nnTTC Cuts at: 0 371 2586 Size: 371 2215 Enzymes that do cut: AccI AcuI AflIII AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanII BbsI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrDI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI EarI Eco57MI EcoRI FalI Hin4I HincII HindIII HpaI MmeI MslI MspA1I NspI PasI PciI PflMI PpiI PspOMI PvuII RsrII SapI SexAI SfcI SmlI SspI StuI StyI TaqII Tth111I XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AscI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI BplI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI BstEII BstXI BstZ17I ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI TstI XhoI XmaI ZraI