Restriction map for
pF1KE2640
(Linear) (Six-base) MAPSORT of: KIEE2640.seq Check: 3187 from: 1 to: 1182
KIEE2640.orf
With 182 enzymes: SgfI *
October 5, 2016 15:26 ..
AccI GT'mk_AC
Cuts at: 0 677 896 1182
Size: 677 219 286
Fragments arranged by size:
677 286 219
Acc65I G'GTAC_C
Cuts at: 0 410 1182
Size: 410 772
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 1182
Size: 651 531
AflIII A'CryG_T
Cuts at: 0 458 1010 1182
Size: 458 552 172
Fragments arranged by size:
552 458 172
AleI CACnn'nnGTG
Cuts at: 0 403 1015 1182
Size: 403 612 167
Fragments arranged by size:
612 403 167
AlwNI CAG_nnn'CTG
Cuts at: 0 120 141 381 639 1182
Size: 120 21 240 258 543
Fragments arranged by size:
543 258 240 120 21
ApaLI G'TGCA_C
Cuts at: 0 39 1182
Size: 39 1143
AscI GG'CGCG_CC
Cuts at: 0 450 1182
Size: 450 732
AvaI C'yCGr_G
Cuts at: 0 97 1182
Size: 97 1085
BanI G'GyrC_C
Cuts at: 0 64 297 410 520 706 1095 1182
Size: 64 233 113 110 186 389 87
Fragments arranged by size:
389 233 186 113 110 87 64
BanII G_rGCy'C
Cuts at: 0 98 1177 1182
Size: 98 1079 5
Fragments arranged by size:
1079 98 5
BbeI G_GCGC'C
Cuts at: 0 68 524 710 1099 1182
Size: 68 456 186 389 83
Fragments arranged by size:
456 389 186 83 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 66 1182
Size: 66 1116
BbvCI CC'TCA_GC
Cuts at: 0 160 1182
Size: 160 1022
BclI T'GATC_A
Cuts at: 0 980 1182
Size: 980 202
BglI GCCn_nnn'nGGC
Cuts at: 0 170 1182
Size: 170 1012
Bme1580I G_kGCm'C
Cuts at: 0 43 293 1182
Size: 43 250 889
Fragments arranged by size:
889 250 43
BmgBI CAC'GTC
Cuts at: 0 459 1004 1182
Size: 459 545 178
Fragments arranged by size:
545 459 178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 142 205 1182
Size: 106 36 63 977
Fragments arranged by size:
977 106 63 36
Bpu10I CC'TnA_GC
Cuts at: 0 160 1182
Size: 160 1022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1182
Size: 190 992
BsaAI yAC'GTr
Cuts at: 0 615 660 1013 1182
Size: 615 45 353 169
Fragments arranged by size:
615 353 169 45
BsaHI Gr'CG_yC
Cuts at: 0 65 521 707 770 1096 1182
Size: 65 456 186 63 326 86
Fragments arranged by size:
456 326 186 86 65 63
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 1182
Size: 193 989
BseYI C'CCAG_C
Cuts at: 0 301 345 438 1182
Size: 301 44 93 744
Fragments arranged by size:
744 301 93 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 403 895 1107 1117 1182
Size: 247 156 492 212 10 65
Fragments arranged by size:
492 247 212 156 65 10
BsiEI CG_ry'CG
Cuts at: 0 1053 1182
Size: 1053 129
BsiHKAI G_wGCw'C
Cuts at: 0 43 482 548 779 931 1177 1182
Size: 43 439 66 231 152 246 5
Fragments arranged by size:
439 246 231 152 66 43 5
Bsp1286I G_dGCh'C
Cuts at: 0 43 98 293 482 548 779 931 1177
Size: 43 55 195 189 66 231 152 246
Cuts at: 1177 1182
Size: 5
Fragments arranged by size:
246 231 195 189 152 66 55 43
5
BspHI T'CATG_A
Cuts at: 0 737 1160 1182
Size: 737 423 22
Fragments arranged by size:
737 423 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 422 1182
Size: 62 360 760
Fragments arranged by size:
760 360 62
BsrBI CCG'CTC
Cuts at: 0 111 357 1182
Size: 111 246 825
Fragments arranged by size:
825 246 111
BsrFI r'CCGG_y
Cuts at: 0 220 241 1182
Size: 220 21 941
Fragments arranged by size:
941 220 21
BssHII G'CGCG_C
Cuts at: 0 148 450 782 833 1182
Size: 148 302 332 51 349
Fragments arranged by size:
349 332 302 148 51
BssSI C'ACGA_G
Cuts at: 0 495 721 930 1182
Size: 495 226 209 252
Fragments arranged by size:
495 252 226 209
BstAPI GCAn_nnn'nTGC
Cuts at: 0 15 141 512 1182
Size: 15 126 371 670
Fragments arranged by size:
670 371 126 15
BtgI C'CryG_G
Cuts at: 0 68 464 860 988 1124 1152 1182
Size: 68 396 396 128 136 28 30
Fragments arranged by size:
396 396 136 128 68 30 28
BtsI GCAGTG_nn'
Cuts at: 0 235 1182
Size: 235 947
DraIII CAC_nnn'GTG
Cuts at: 0 512 1182
Size: 512 670
EaeI y'GGCC_r
Cuts at: 0 467 1050 1182
Size: 467 583 132
Fragments arranged by size:
583 467 132
EagI C'GGCC_G
Cuts at: 0 1050 1182
Size: 1050 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 833 1095 1182
Size: 833 262 87
Fragments arranged by size:
833 262 87
EcoICRI GAG'CTC
Cuts at: 0 1175 1182
Size: 1175 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 142 205 651 1182
Size: 106 36 63 446 531
Fragments arranged by size:
531 446 106 63 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 163 405 1182
Size: 163 242 777
Fragments arranged by size:
777 242 163
EcoO109I rG'GnC_Cy
Cuts at: 0 636 993 1182
Size: 636 357 189
Fragments arranged by size:
636 357 189
FspI TGC'GCA
Cuts at: 0 19 1182
Size: 19 1163
FspAI rTGC'GCAy
Cuts at: 0 19 1182
Size: 19 1163
HaeII r_GCGC'y
Cuts at: 0 31 68 237 524 622 710 1099 1182
Size: 31 37 169 287 98 88 389 83
Fragments arranged by size:
389 287 169 98 88 83 37 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 838 870 1054 1086 1182
Size: 838 32 184 32 96
Fragments arranged by size:
838 184 96 32 32
KasI G'GCGC_C
Cuts at: 0 64 520 706 1095 1182
Size: 64 456 186 389 87
Fragments arranged by size:
456 389 186 87 64
KpnI G_GTAC'C
Cuts at: 0 414 1182
Size: 414 768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1031 1182
Size: 71 960 151
Fragments arranged by size:
960 151 71
MscI TGG'CCA
Cuts at: 0 469 1182
Size: 469 713
MslI CAynn'nnrTG
Cuts at: 0 403 742 976 1015 1182
Size: 403 339 234 39 167
Fragments arranged by size:
403 339 234 167 39
MspA1I CmG'CkG
Cuts at: 0 70 117 871 990 1091 1154 1182
Size: 70 47 754 119 101 63 28
Fragments arranged by size:
754 119 101 70 63 47 28
NaeI GCC'GGC
Cuts at: 0 222 243 1182
Size: 222 21 939
Fragments arranged by size:
939 222 21
NarI GG'CG_CC
Cuts at: 0 65 521 707 1096 1182
Size: 65 456 186 389 86
Fragments arranged by size:
456 389 186 86 65
NcoI C'CATG_G
Cuts at: 0 464 1182
Size: 464 718
NgoMIV G'CCGG_C
Cuts at: 0 220 241 1182
Size: 220 21 941
Fragments arranged by size:
941 220 21
NotI GC'GGCC_GC
Cuts at: 0 1050 1182
Size: 1050 132
NruI TCG'CGA
Cuts at: 0 1074 1182
Size: 1074 108
NspI r_CATG'y
Cuts at: 0 333 447 1182
Size: 333 114 735
Fragments arranged by size:
735 333 114
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 1182
Size: 381 801
PmlI CAC'GTG
Cuts at: 0 1013 1182
Size: 1013 169
PpuMI rG'GwC_Cy
Cuts at: 0 993 1182
Size: 993 189
PstI C_TGCA'G
Cuts at: 0 626 644 965 1182
Size: 626 18 321 217
Fragments arranged by size:
626 321 217 18
SacI G_AGCT'C
Cuts at: 0 1177 1182
Size: 1177 5
SacII CC_GC'GG
Cuts at: 0 71 991 1155 1182
Size: 71 920 164 27
Fragments arranged by size:
920 164 71 27
ScaI AGT'ACT
Cuts at: 0 967 1182
Size: 967 215
SfcI C'TryA_G
Cuts at: 0 622 640 961 1056 1182
Size: 622 18 321 95 126
Fragments arranged by size:
622 321 126 95 18
SfoI GGC'GCC
Cuts at: 0 66 522 708 1097 1182
Size: 66 456 186 389 85
Fragments arranged by size:
456 389 186 85 66
SmlI C'TyrA_G
Cuts at: 0 205 1182
Size: 205 977
SphI G_CATG'C
Cuts at: 0 447 1182
Size: 447 735
StuI AGG'CCT
Cuts at: 0 565 1182
Size: 565 617
StyI C'CwwG_G
Cuts at: 0 401 464 1182
Size: 401 63 718
Fragments arranged by size:
718 401 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 315 1182
Size: 315 867
TatI w'GTAC_w
Cuts at: 0 965 1182
Size: 965 217
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 904 936 1126 1158 1182
Size: 904 32 190 32 24
Fragments arranged by size:
904 190 32 32 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 435 471 1182
Size: 435 36 711
Fragments arranged by size:
711 435 36
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlwNI ApaLI AscI
AvaI BanI BanII BbeI BbsI BbvCI BclI BglI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BtgI BtsI DraIII EaeI
EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI
HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NruI NspI PflMI
PmlI PpuMI PstI SacI SacII ScaI SfcI SfoI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApoI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BfrBI BglII BlpI BmrI BmtI BplI
BsaI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BsrDI BsrGI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EarI EcoRI EcoRV
FalI FseI HincII HindIII HpaI MfeI MluI NdeI
NheI NsiI PacI PasI PciI PfoI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI