Restriction map for
pF1KE2646
(Linear) (Six-base) MAPSORT of: KIEE2646.seq Check: 1133 from: 1 to: 6747
KIEE2646.orf
With 182 enzymes: SgfI *
November 11, 2016 16:52 ..
AatII G_ACGT'C
Cuts at: 0 5639 6747
Size: 5639 1108
AccI GT'mk_AC
Cuts at: 0 3218 6575 6747
Size: 3218 3357 172
Fragments arranged by size:
3357 3218 172
Acc65I G'GTAC_C
Cuts at: 0 866 1910 4500 6747
Size: 866 1044 2590 2247
Fragments arranged by size:
2590 2247 1044 866
AclI AA'CG_TT
Cuts at: 0 3389 6747
Size: 3389 3358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3483 3649 5615 5687 6747
Size: 3483 166 1966 72 1060
Fragments arranged by size:
3483 1966 1060 166 72
AfeI AGC'GCT
Cuts at: 0 708 1210 4681 6747
Size: 708 502 3471 2066
Fragments arranged by size:
3471 2066 708 502
AflII C'TTAA_G
Cuts at: 0 5547 6747
Size: 5547 1200
AflIII A'CryG_T
Cuts at: 0 1928 3923 4169 6068 6747
Size: 1928 1995 246 1899 679
Fragments arranged by size:
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AhdI GACnn_n'nnGTC
Cuts at: 0 2519 3310 5163 6747
Size: 2519 791 1853 1584
Fragments arranged by size:
2519 1853 1584 791
AleI CACnn'nnGTG
Cuts at: 0 3175 6747
Size: 3175 3572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2873 3811 4426 6151 6747
Size: 2873 938 615 1725 596
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 1887 3028 3444 6747
Size: 1887 1141 416 3303
Fragments arranged by size:
3303 1887 1141 416
ApaI G_GGCC'C
Cuts at: 0 163 526 828 1144 2203 2615 4145 4240
Size: 163 363 302 316 1059 412 1530 95
Cuts at: 4240 4303 4441 5687 6747
Size: 63 138 1246 1060
Fragments arranged by size:
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163 138 95 63
ApaLI G'TGCA_C
Cuts at: 0 3706 6747
Size: 3706 3041
ApoI r'AATT_y
Cuts at: 0 5087 5516 6328 6640 6747
Size: 5087 429 812 312 107
Fragments arranged by size:
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AscI GG'CGCG_CC
Cuts at: 0 437 6747
Size: 437 6310
AseI AT'TA_AT
Cuts at: 0 4973 6747
Size: 4973 1774
AvaI C'yCGr_G
Cuts at: 0 162 519 606 652 781 1144 1312 1699
Size: 162 357 87 46 129 363 168 387
Cuts at: 1699 2193 2305 3118 3555 4056 4233 4302 6049
Size: 494 112 813 437 501 177 69 1747
Cuts at: 6049 6747
Size: 698
Fragments arranged by size:
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357 177 168 162 129 112 87 69
46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1654 1687 6747
Size: 1654 33 5060
Fragments arranged by size:
5060 1654 33
BamHI G'GATC_C
Cuts at: 0 1554 3559 6747
Size: 1554 2005 3188
Fragments arranged by size:
3188 2005 1554
BanI G'GyrC_C
Cuts at: 0 16 34 481 616 866 1438 1510 1910
Size: 16 18 447 135 250 572 72 400
Cuts at: 1910 2583 2979 2997 3394 4079 4458 4500 4656
Size: 673 396 18 397 685 379 42 156
Cuts at: 4656 5531 6747
Size: 875 1216
Fragments arranged by size:
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396 379 250 156 135 72 42 18
18 16
BanII G_rGCy'C
Cuts at: 0 163 169 526 828 1081 1108 1144 1507
Size: 163 6 357 302 253 27 36 363
Cuts at: 1507 2092 2203 2615 3418 3485 3830 3881 4145
Size: 585 111 412 803 67 345 51 264
Cuts at: 4145 4240 4303 4441 5687 5768 6747
Size: 95 63 138 1246 81 979
Fragments arranged by size:
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302 264 253 163 138 111 95 81
67 63 51 36 27 6
BbeI G_GCGC'C
Cuts at: 0 20 485 620 1442 2983 3001 4462 4660
Size: 20 465 135 822 1541 18 1461 198
Cuts at: 4660 5535 6747
Size: 875 1212
Fragments arranged by size:
1541 1461 1212 875 822 465 198 135
20 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 2000 3489 5234 5255 5266 5675 6747
Size: 2000 1489 1745 21 11 409 1072
Fragments arranged by size:
2000 1745 1489 1072 409 21 11
BbvCI CC'TCA_GC
Cuts at: 0 443 1168 1616 1727 4379 4575 6747
Size: 443 725 448 111 2652 196 2172
Fragments arranged by size:
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BciVI GTATCCnnnnn_n'
Cuts at: 0 1682 2284 4046 4283 5728 6280 6747
Size: 1682 602 1762 237 1445 552 467
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 756 5133 6747
Size: 756 4377 1614
Fragments arranged by size:
4377 1614 756
BfrBI ATG'CAT
Cuts at: 0 2170 2472 2646 5834 6747
Size: 2170 302 174 3188 913
Fragments arranged by size:
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BglI GCCn_nnn'nGGC
Cuts at: 0 734 823 911 1033 1148 1189 1462 1471
Size: 734 89 88 122 115 41 273 9
Cuts at: 1471 1658 1823 4163 4445 6747
Size: 187 165 2340 282 2302
Fragments arranged by size:
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115 89 88 41 9
BglII A'GATC_T
Cuts at: 0 1954 2037 6747
Size: 1954 83 4710
Fragments arranged by size:
4710 1954 83
BlpI GC'TnA_GC
Cuts at: 0 535 1102 2091 5825 6747
Size: 535 567 989 3734 922
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 163 526 628 653 828 1144 2203 2588
Size: 163 363 102 25 175 316 1059 385
Cuts at: 2588 2615 2684 3710 4082 4145 4240 4303 4441
Size: 27 69 1026 372 63 95 63 138
Cuts at: 4441 5687 6175 6747
Size: 1246 488 572
Fragments arranged by size:
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316 175 163 138 102 95 69 63
63 27 25
BmgBI CAC'GTC
Cuts at: 0 4545 6747
Size: 4545 2202
BmrI ACTGGGnnnn_n'
Cuts at: 0 1353 1437 1890 2582 2747 3167 3579 5016
Size: 1353 84 453 692 165 420 412 1437
Cuts at: 5016 6367 6504 6747
Size: 1351 137 243
Fragments arranged by size:
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165 137 84
BmtI G_CTAG'C
Cuts at: 0 6610 6747
Size: 6610 137
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3267 3269 6747
Size: 3267 2 3478
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1640 1763 2169 2544 3517 4350 4635 4708
Size: 1640 123 406 375 973 833 285 73
Cuts at: 4708 5776 6268 6747
Size: 1068 492 479
Fragments arranged by size:
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285 123 73
Bpu10I CC'TnA_GC
Cuts at: 0 443 1168 1616 1727 4379 4575 6747
Size: 443 725 448 111 2652 196 2172
Fragments arranged by size:
2652 2172 725 448 443 196 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 2361 2490 3246 3384 6078 6747
Size: 58 2303 129 756 138 2694 669
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 1566 5105 6244 6747
Size: 1566 3539 1139 503
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 4955 6747
Size: 4955 1792
BsaHI Gr'CG_yC
Cuts at: 0 17 482 617 1439 2980 2998 4459 4624
Size: 17 465 135 822 1541 18 1461 165
Cuts at: 4624 4657 5306 5532 5636 6747
Size: 33 649 226 104 1111
Fragments arranged by size:
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135 104 33 18 17
BsaWI w'CCGG_w
Cuts at: 0 3943 5738 6747
Size: 3943 1795 1009
Fragments arranged by size:
3943 1795 1009
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 790 820 4809 4839 6747
Size: 790 30 3989 30 1908
Fragments arranged by size:
3989 1908 790 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 96 99 102 105 108 141 959 962
Size: 96 3 3 3 3 33 818 3
Cuts at: 962 965 977 980 983 986 989 992 995
Size: 3 12 3 3 3 3 3 3
Cuts at: 995 1010 1955 3135 3493 5206 5248 5257 5513
Size: 15 945 1180 358 1713 42 9 256
Cuts at: 5513 5777 6007 6296 6747
Size: 264 230 289 451
Fragments arranged by size:
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256 230 96 42 33 15 12 9
3 3 3 3 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 742 1276 1327 1901 2568 2704 3186 3642
Size: 742 534 51 574 667 136 482 456
Cuts at: 3642 4146 4660 4665 5116 6747
Size: 504 514 5 451 1631
Fragments arranged by size:
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456 451 136 51 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3428 5434 5446 6142 6472 6606 6747
Size: 3428 2006 12 696 330 134 141
Fragments arranged by size:
3428 2006 696 330 141 134 12
BsiEI CG_ry'CG
Cuts at: 0 26 594 644 677 738 1028 1181 1409
Size: 26 568 50 33 61 290 153 228
Cuts at: 1409 1475 3222 4008 6747
Size: 66 1747 786 2739
Fragments arranged by size:
2739 1747 786 568 290 228 153 66
61 50 33 26
BsiHKAI G_wGCw'C
Cuts at: 0 605 1108 2092 3418 3710 5486 5973 6747
Size: 605 503 984 1326 292 1776 487 774
Fragments arranged by size:
1776 1326 984 774 605 503 487 292
BsmI GAATG_Cn'
Cuts at: 0 1721 2699 6747
Size: 1721 978 4048
Fragments arranged by size:
4048 1721 978
BsmBI CGTCTCn'nnnn_
Cuts at: 0 172 5771 6747
Size: 172 5599 976
Fragments arranged by size:
5599 976 172
Bsp1286I G_dGCh'C
Cuts at: 0 163 169 526 605 628 653 828 1081
Size: 163 6 357 79 23 25 175 253
Cuts at: 1081 1108 1144 1507 2092 2203 2588 2615 2684
Size: 27 36 363 585 111 385 27 69
Cuts at: 2684 3418 3485 3710 3830 3881 4082 4145 4240
Size: 734 67 225 120 51 201 63 95
Cuts at: 4240 4303 4441 5486 5687 5768 5973 6175 6747
Size: 63 138 1045 201 81 205 202 572
Fragments arranged by size:
1045 734 585 572 385 363 357 253
225 205 202 201 201 175 163 138
120 111 95 81 79 69 67 63
63 51 36 27 27 25 23 6
BspEI T'CCGG_A
Cuts at: 0 3943 6747
Size: 3943 2804
BspHI T'CATG_A
Cuts at: 0 4521 6747
Size: 4521 2226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 5548 6747
Size: 5548 1199
BsrBI CCG'CTC
Cuts at: 0 53 722 1006 6635 6747
Size: 53 669 284 5629 112
Fragments arranged by size:
5629 669 284 112 53
BsrDI GCAATG_nn'
Cuts at: 0 3956 4410 5659 5886 6747
Size: 3956 454 1249 227 861
Fragments arranged by size:
3956 1249 861 454 227
BsrFI r'CCGG_y
Cuts at: 0 31 582 639 905 1183 1216 6659 6747
Size: 31 551 57 266 278 33 5443 88
Fragments arranged by size:
5443 551 278 266 88 57 33 31
BsrGI T'GTAC_A
Cuts at: 0 3926 4172 4208 6747
Size: 3926 246 36 2539
Fragments arranged by size:
3926 2539 246 36
BssHII G'CGCG_C
Cuts at: 0 437 1331 6747
Size: 437 894 5416
Fragments arranged by size:
5416 894 437
BssSI C'ACGA_G
Cuts at: 0 2115 4159 5947 6652 6747
Size: 2115 2044 1788 705 95
Fragments arranged by size:
2115 2044 1788 705 95
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1887 2101 6747
Size: 1887 214 4646
Fragments arranged by size:
4646 1887 214
BstEII G'GTnAC_C
Cuts at: 0 3622 4773 6747
Size: 3622 1151 1974
Fragments arranged by size:
3622 1974 1151
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2647 2671 3592 5029 6747
Size: 2647 24 921 1437 1718
Fragments arranged by size:
2647 1718 1437 921 24
BstYI r'GATC_y
Cuts at: 0 1534 1554 1954 2037 3559 4310 6192 6747
Size: 1534 20 400 83 1522 751 1882 555
Fragments arranged by size:
1882 1534 1522 751 555 400 83 20
Bsu36I CC'TnA_GG
Cuts at: 0 4901 6747
Size: 4901 1846
BtgI C'CryG_G
Cuts at: 0 20 122 173 1028 1085 1409 1526 1706
Size: 20 102 51 855 57 324 117 180
Cuts at: 1706 2066 3005 3275 3605 4754 6392 6611 6747
Size: 360 939 270 330 1149 1638 219 136
Fragments arranged by size:
1638 1149 939 855 360 330 324 270
219 180 136 117 102 57 51 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1513 5271 5285 5784 5844 6747
Size: 1513 3758 14 499 60 903
Fragments arranged by size:
3758 1513 903 499 60 14
BtsI GCAGTG_nn'
Cuts at: 0 2849 3411 4633 6747
Size: 2849 562 1222 2114
Fragments arranged by size:
2849 2114 1222 562
ClaI AT'CG_AT
Cuts at: 0 5630 6747
Size: 5630 1117
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 63 98 6747
Size: 63 35 6649
Fragments arranged by size:
6649 63 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 3585 4629 6747
Size: 3585 1044 2118
Fragments arranged by size:
3585 2118 1044
EaeI y'GGCC_r
Cuts at: 0 23 170 504 591 674 735 1025 1178
Size: 23 147 334 87 83 61 290 153
Cuts at: 1178 1218 1395 1406 1472 1709 3660 5650 6083
Size: 40 177 11 66 237 1951 1990 433
Cuts at: 6083 6299 6593 6614 6747
Size: 216 294 21 133
Fragments arranged by size:
1990 1951 433 334 294 290 237 216
177 153 147 133 87 83 66 61
40 23 21 11
EagI C'GGCC_G
Cuts at: 0 23 591 674 735 1025 1178 1406 1472
Size: 23 568 83 61 290 153 228 66
Cuts at: 1472 6747
Size: 5275
Fragments arranged by size:
5275 568 290 228 153 83 66 61
23
EarI CTCTTCn'nnn_
Cuts at: 0 150 1564 3236 5591 6747
Size: 150 1414 1672 2355 1156
Fragments arranged by size:
2355 1672 1414 1156 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 782 792 798 956 6747
Size: 782 10 6 158 5791
Fragments arranged by size:
5791 782 158 10 6
EcoICRI GAG'CTC
Cuts at: 0 1106 2090 3416 6747
Size: 1106 984 1326 3331
Fragments arranged by size:
3331 1326 1106 984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1640 1763 2169 2544 3483 3517 3649 4350
Size: 1640 123 406 375 939 34 132 701
Cuts at: 4350 4635 4708 5615 5687 5776 6268 6747
Size: 285 73 907 72 89 492 479
Fragments arranged by size:
1640 939 907 701 492 479 406 375
285 132 123 89 73 72 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1171 4281 4469 6747
Size: 1171 3110 188 2278
Fragments arranged by size:
3110 2278 1171 188
EcoO109I rG'GnC_Cy
Cuts at: 0 824 825 1141 2199 2611 3837 4048 4142
Size: 824 1 316 1058 412 1226 211 94
Cuts at: 4142 4299 4375 4437 4438 5684 6211 6747
Size: 157 76 62 1 1246 527 536
Fragments arranged by size:
1246 1226 1058 824 536 527 412 316
211 157 94 76 62 1 1
EcoRI G'AATT_C
Cuts at: 0 5087 6640 6747
Size: 5087 1553 107
Fragments arranged by size:
5087 1553 107
EcoRV GAT'ATC
Cuts at: 0 4890 6672 6680 6747
Size: 4890 1782 8 67
Fragments arranged by size:
4890 1782 67 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2531 3858 5727 6747
Size: 2531 1327 1869 1020
Fragments arranged by size:
2531 1869 1327 1020
HaeII r_GCGC'y
Cuts at: 0 20 47 92 485 620 710 1212 1442
Size: 20 27 45 393 135 90 502 230
Cuts at: 1442 2983 3001 3978 4158 4462 4660 4683 5535
Size: 1541 18 977 180 304 198 23 852
Cuts at: 5535 6400 6747
Size: 865 347
Fragments arranged by size:
1541 977 865 852 502 393 347 304
230 198 180 135 90 45 27 23
20 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1242 1274 1767 1799 2505 2537 3034 3066
Size: 1242 32 493 32 706 32 497 32
Cuts at: 3066 3112 3144 3296 3328 3703 3735 4503 4535
Size: 46 32 152 32 375 32 768 32
Cuts at: 4535 5149 5181 5626 5658 6747
Size: 614 32 445 32 1089
Fragments arranged by size:
1242 1089 768 706 614 497 493 445
375 152 46 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3219 3590 5400 6747
Size: 3219 371 1810 1347
Fragments arranged by size:
3219 1810 1347 371
HindIII A'AGCT_T
Cuts at: 0 6480 6747
Size: 6480 267
KasI G'GCGC_C
Cuts at: 0 16 481 616 1438 2979 2997 4458 4656
Size: 16 465 135 822 1541 18 1461 198
Cuts at: 4656 5531 6747
Size: 875 1216
Fragments arranged by size:
1541 1461 1216 875 822 465 198 135
18 16
KpnI G_GTAC'C
Cuts at: 0 870 1914 4504 6747
Size: 870 1044 2590 2243
Fragments arranged by size:
2590 2243 1044 870
MfeI C'AATT_G
Cuts at: 0 1676 6747
Size: 1676 5071
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1672 3327 6226 6747
Size: 428 1244 1655 2899 521
Fragments arranged by size:
2899 1655 1244 521 428
MscI TGG'CCA
Cuts at: 0 6085 6301 6616 6747
Size: 6085 216 315 131
Fragments arranged by size:
6085 315 216 131
MslI CAynn'nnrTG
Cuts at: 0 263 284 1135 3175 3235 3793 5839 6448
Size: 263 21 851 2040 60 558 2046 609
Cuts at: 6448 6541 6747
Size: 93 206
Fragments arranged by size:
2046 2040 851 609 558 263 206 93
60 21
MspA1I CmG'CkG
Cuts at: 0 22 124 133 531 959 1030 1087 1263
Size: 22 102 9 398 428 71 57 176
Cuts at: 1263 1411 1453 1668 2603 2704 4353 5311 6685
Size: 148 42 215 935 101 1649 958 1374
Cuts at: 6685 6747
Size: 62
Fragments arranged by size:
1649 1374 958 935 428 398 215 176
148 102 101 71 62 57 42 22
9
NaeI GCC'GGC
Cuts at: 0 33 584 907 1185 1218 6747
Size: 33 551 323 278 33 5529
Fragments arranged by size:
5529 551 323 278 33 33
NarI GG'CG_CC
Cuts at: 0 17 482 617 1439 2980 2998 4459 4657
Size: 17 465 135 822 1541 18 1461 198
Cuts at: 4657 5532 6747
Size: 875 1215
Fragments arranged by size:
1541 1461 1215 875 822 465 198 135
18 17
NcoI C'CATG_G
Cuts at: 0 173 1526 1706 3005 3605 6392 6611 6747
Size: 173 1353 180 1299 600 2787 219 136
Fragments arranged by size:
2787 1353 1299 600 219 180 173 136
NdeI CA'TA_TG
Cuts at: 0 4124 6747
Size: 4124 2623
NgoMIV G'CCGG_C
Cuts at: 0 31 582 905 1183 1216 6747
Size: 31 551 323 278 33 5531
Fragments arranged by size:
5531 551 323 278 33 31
NheI G'CTAG_C
Cuts at: 0 6606 6747
Size: 6606 141
NotI GC'GGCC_GC
Cuts at: 0 23 591 735 1025 1178 1406 1472 6747
Size: 23 568 144 290 153 228 66 5275
Fragments arranged by size:
5275 568 290 228 153 144 66 23
NsiI A_TGCA'T
Cuts at: 0 2172 2474 2648 5836 6747
Size: 2172 302 174 3188 911
Fragments arranged by size:
3188 2172 911 302 174
NspI r_CATG'y
Cuts at: 0 1932 2170 2476 2769 3798 3918 3927 4137
Size: 1932 238 306 293 1029 120 9 210
Cuts at: 4137 4173 4182 4701 6546 6747
Size: 36 9 519 1845 201
Fragments arranged by size:
1932 1845 1029 519 306 293 238 210
201 120 36 9 9
PasI CC'CwG_GG
Cuts at: 0 621 1539 2608 2685 5680 6747
Size: 621 918 1069 77 2995 1067
Fragments arranged by size:
2995 1069 1067 918 621 77
PciI A'CATG_T
Cuts at: 0 1928 3923 4169 6747
Size: 1928 1995 246 2578
Fragments arranged by size:
2578 1995 1928 246
PflMI CCAn_nnn'nTGG
Cuts at: 0 2704 2728 5169 6747
Size: 2704 24 2441 1578
Fragments arranged by size:
2704 2441 1578 24
PfoI T'CCnGG_A
Cuts at: 0 680 2727 6272 6747
Size: 680 2047 3545 475
Fragments arranged by size:
3545 2047 680 475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1941 1973 2941 2973 6747
Size: 1941 32 968 32 3774
Fragments arranged by size:
3774 1941 968 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 3837 6211 6747
Size: 3837 2374 536
Fragments arranged by size:
3837 2374 536
PspOMI G'GGCC_C
Cuts at: 0 159 522 824 1140 2199 2611 4141 4236
Size: 159 363 302 316 1059 412 1530 95
Cuts at: 4236 4299 4437 5683 6747
Size: 63 138 1246 1064
Fragments arranged by size:
1530 1246 1064 1059 412 363 316 302
159 138 95 63
PspXI vC'TCGA_Gb
Cuts at: 0 6049 6747
Size: 6049 698
PstI C_TGCA'G
Cuts at: 0 851 4358 5316 6022 6183 6747
Size: 851 3507 958 706 161 564
Fragments arranged by size:
3507 958 851 706 564 161
PvuII CAG'CTG
Cuts at: 0 1263 2704 6685 6747
Size: 1263 1441 3981 62
Fragments arranged by size:
3981 1441 1263 62
RsrII CG'GwC_CG
Cuts at: 0 1519 4242 6747
Size: 1519 2723 2505
Fragments arranged by size:
2723 2505 1519
SacI G_AGCT'C
Cuts at: 0 1108 2092 3418 6747
Size: 1108 984 1326 3329
Fragments arranged by size:
3329 1326 1108 984
SacII CC_GC'GG
Cuts at: 0 23 125 1031 1088 1412 6747
Size: 23 102 906 57 324 5335
Fragments arranged by size:
5335 906 324 102 57 23
SalI G'TCGA_C
Cuts at: 0 3217 6747
Size: 3217 3530
SanDI GG'GwC_CC
Cuts at: 0 6211 6747
Size: 6211 536
SapI GCTCTTCn'nnn_
Cuts at: 0 5591 6747
Size: 5591 1156
ScaI AGT'ACT
Cuts at: 0 5056 6747
Size: 5056 1691
SfcI C'TryA_G
Cuts at: 0 316 847 900 2228 2598 2934 2988 4278
Size: 316 531 53 1328 370 336 54 1290
Cuts at: 4278 4354 5312 6018 6179 6747
Size: 76 958 706 161 568
Fragments arranged by size:
1328 1290 958 706 568 531 370 336
316 161 76 54 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 734 1471 1658 4445 6747
Size: 734 737 187 2787 2302
Fragments arranged by size:
2787 2302 737 734 187
SfoI GGC'GCC
Cuts at: 0 18 483 618 1440 2981 2999 4460 4658
Size: 18 465 135 822 1541 18 1461 198
Cuts at: 4658 5533 6747
Size: 875 1214
Fragments arranged by size:
1541 1461 1214 875 822 465 198 135
18 18
SmaI CCC'GGG
Cuts at: 0 164 521 608 654 783 1146 1701 2195
Size: 164 357 87 46 129 363 555 494
Cuts at: 2195 2307 4304 6747
Size: 112 1997 2443
Fragments arranged by size:
2443 1997 555 494 363 357 164 129
112 87 46
SmlI C'TyrA_G
Cuts at: 0 73 2340 2505 3261 3399 5547 6049 6057
Size: 73 2267 165 756 138 2148 502 8
Cuts at: 6057 6747
Size: 690
Fragments arranged by size:
2267 2148 756 690 502 165 138 73
8
SphI G_CATG'C
Cuts at: 0 2476 3798 3918 6747
Size: 2476 1322 120 2829
Fragments arranged by size:
2829 2476 1322 120
SrfI GCCC'GGGC
Cuts at: 0 164 6747
Size: 164 6583
SspI AAT'ATT
Cuts at: 0 4977 5850 6747
Size: 4977 873 897
Fragments arranged by size:
4977 897 873
StuI AGG'CCT
Cuts at: 0 5909 6747
Size: 5909 838
StyI C'CwwG_G
Cuts at: 0 173 485 1526 1706 2124 3005 3605 4044
Size: 173 312 1041 180 418 881 600 439
Cuts at: 4044 5168 6392 6417 6596 6611 6747
Size: 1124 1224 25 179 15 136
Fragments arranged by size:
1224 1124 1041 881 600 439 418 312
180 179 173 136 25 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 52 1536 3236 4022 5417 5707 6210 6747
Size: 52 1484 1700 786 1395 290 503 537
Fragments arranged by size:
1700 1484 1395 786 537 503 290 52
TatI w'GTAC_w
Cuts at: 0 1316 3694 3926 4172 4184 4208 5054 6723
Size: 1316 2378 232 246 12 24 846 1669
Cuts at: 6723 6747
Size: 24
Fragments arranged by size:
2378 1669 1316 846 246 232 24 24
12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 790 822 4310 4342 4809 4841 6747
Size: 790 32 3488 32 467 32 1906
Fragments arranged by size:
3488 1906 790 467 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2248 2473 6618 6747
Size: 2248 225 4145 129
Fragments arranged by size:
4145 2248 225 129
XhoI C'TCGA_G
Cuts at: 0 6049 6747
Size: 6049 698
XmaI C'CCGG_G
Cuts at: 0 162 519 606 652 781 1144 1699 2193
Size: 162 357 87 46 129 363 555 494
Cuts at: 2193 2305 4302 6747
Size: 112 1997 2445
Fragments arranged by size:
2445 1997 555 494 363 357 162 129
112 87 46
ZraI GAC'GTC
Cuts at: 0 5637 6747
Size: 5637 1110
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PpiI PpuMI PspOMI PspXI PstI
PvuII RsrII SacI SacII SalI SanDI SapI ScaI
SfcI SfiI SfoI SmaI SmlI SphI SrfI SspI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AarI AgeI AloI AsiSI AvrII BcgI BsaBI BsiWI
BstBI BstZ17I DraI DraIII FseI FspI FspAI HpaI
MluI NruI PacI PmeI PmlI PshAI PsiI PsrI
PvuI SbfI SexAI SgfI SgrAI SnaBI SpeI SwaI
Tth111I XbaI XmnI