(Linear) (Six-base) MAPSORT of: KIEE2649.seq  Check: 1849  from: 1  to: 573



KIEE2649.orf

 With 182 enzymes: SgfI * 

                        November 11, 2016 16:52  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     548     573 
   Size:        548      25
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     573 
   Size:        466     107
 
AflIII A'CryG_T

Cuts at:      0      49     573 
   Size:         49     524
 
AlwNI CAG_nnn'CTG

Cuts at:      0     421     573 
   Size:        421     152
 
ApoI r'AATT_y

Cuts at:      0      79     573 
   Size:         79     494
 
BanI G'GyrC_C

Cuts at:      0     291     573 
   Size:        291     282
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     398     521     550     573 
   Size:        398     123      29      23

  Fragments arranged by size: 

                 398     123      29      23
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     258     573 
   Size:        258     315
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     573 
   Size:        458     115
 
BsiEI CG_ry'CG

Cuts at:      0     205     573 
   Size:        205     368
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     548     573 
   Size:        548      25
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      44     573 
   Size:         44     529
 
EarI CTCTTCn'nnn_

Cuts at:      0     544     573 
   Size:        544      29
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     398     466     521     550     573 
   Size:        398      68      55      29      23

  Fragments arranged by size: 

                 398      68      55      29      23
 
EcoRV GAT'ATC

Cuts at:      0      62     573 
   Size:         62     511
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     352     384     573 
   Size:        352      32     189

  Fragments arranged by size: 

                 352     189      32
 
MslI CAynn'nnrTG

Cuts at:      0     130     573 
   Size:        130     443
 
MspA1I CmG'CkG

Cuts at:      0     207     573 
   Size:        207     366
 
NspI r_CATG'y

Cuts at:      0      53     573 
   Size:         53     520
 
PciI A'CATG_T

Cuts at:      0      49     573 
   Size:         49     524
 
PstI C_TGCA'G

Cuts at:      0     177     573 
   Size:        177     396
 
SapI GCTCTTCn'nnn_

Cuts at:      0     544     573 
   Size:        544      29
 
SfcI C'TryA_G

Cuts at:      0     173     573 
   Size:        173     400
 
SspI AAT'ATT

Cuts at:      0     518     573 
   Size:        518      55
 
StuI AGG'CCT

Cuts at:      0     491     573 
   Size:        491      82
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     279     573 
   Size:        279     294
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    AlwNI     ApoI     BanI     BpmI     BsaI
     BsgI    BsiEI    BspMI    BtgZI     EarI  Eco57MI    EcoRV    Hin4I
     MslI   MspA1I     NspI     PciI     PstI     SapI     SfcI     SspI
     StuI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI