Restriction map for
pF1KE2660
(Linear) (Six-base) MAPSORT of: KIEE2660.seq Check: 8122 from: 1 to: 2394
KIEE2660.orf
With 182 enzymes: SgfI *
February 2, 2017 14:35 ..
AatII G_ACGT'C
Cuts at: 0 572 2394
Size: 572 1822
AccI GT'mk_AC
Cuts at: 0 782 2394
Size: 782 1612
Acc65I G'GTAC_C
Cuts at: 0 1427 2394
Size: 1427 967
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1778 2089 2394
Size: 92 1686 311 305
Fragments arranged by size:
1686 311 305 92
AflII C'TTAA_G
Cuts at: 0 1902 2232 2394
Size: 1902 330 162
Fragments arranged by size:
1902 330 162
AflIII A'CryG_T
Cuts at: 0 874 1025 1796 2394
Size: 874 151 771 598
Fragments arranged by size:
874 771 598 151
AhdI GACnn_n'nnGTC
Cuts at: 0 26 2394
Size: 26 2368
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 451 483 2394
Size: 451 32 1911
Fragments arranged by size:
1911 451 32
AlwNI CAG_nnn'CTG
Cuts at: 0 588 2394
Size: 588 1806
ApaI G_GGCC'C
Cuts at: 0 498 2394
Size: 498 1896
ApoI r'AATT_y
Cuts at: 0 102 203 760 2394
Size: 102 101 557 1634
Fragments arranged by size:
1634 557 102 101
AvrII C'CTAG_G
Cuts at: 0 1339 2394
Size: 1339 1055
BamHI G'GATC_C
Cuts at: 0 2059 2394
Size: 2059 335
BanI G'GyrC_C
Cuts at: 0 346 1427 1954 2394
Size: 346 1081 527 440
Fragments arranged by size:
1081 527 440 346
BanII G_rGCy'C
Cuts at: 0 498 1696 2394
Size: 498 1198 698
Fragments arranged by size:
1198 698 498
BbsI GAAGACnn'nnnn_
Cuts at: 0 773 909 2394
Size: 773 136 1485
Fragments arranged by size:
1485 773 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 324 358 2394
Size: 324 34 2036
Fragments arranged by size:
2036 324 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 221 1679 1724 2394
Size: 221 1458 45 670
Fragments arranged by size:
1458 670 221 45
BclI T'GATC_A
Cuts at: 0 500 1572 2394
Size: 500 1072 822
Fragments arranged by size:
1072 822 500
BfrBI ATG'CAT
Cuts at: 0 121 756 2394
Size: 121 635 1638
Fragments arranged by size:
1638 635 121
BglI GCCn_nnn'nGGC
Cuts at: 0 444 2394
Size: 444 1950
BlpI GC'TnA_GC
Cuts at: 0 1072 2394
Size: 1072 1322
Bme1580I G_kGCm'C
Cuts at: 0 498 804 1532 2394
Size: 498 306 728 862
Fragments arranged by size:
862 728 498 306
BmrI ACTGGGnnnn_n'
Cuts at: 0 616 1155 1691 2394
Size: 616 539 536 703
Fragments arranged by size:
703 616 539 536
BmtI G_CTAG'C
Cuts at: 0 637 2394
Size: 637 1757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 439 1206 1554 2300 2394
Size: 363 76 767 348 746 94
Fragments arranged by size:
767 746 363 348 94 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 2394
Size: 82 2312
BsaI GGTCTCn'nnnn_
Cuts at: 0 1081 2394
Size: 1081 1313
BsaAI yAC'GTr
Cuts at: 0 1014 2394
Size: 1014 1380
BsaBI GATnn'nnATC
Cuts at: 0 1099 1882 2064 2394
Size: 1099 783 182 330
Fragments arranged by size:
1099 783 330 182
BsaHI Gr'CG_yC
Cuts at: 0 569 2394
Size: 569 1825
BsaWI w'CCGG_w
Cuts at: 0 366 2394
Size: 366 2028
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 451 481 2260 2290 2394
Size: 451 30 1779 30 104
Fragments arranged by size:
1779 451 104 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 1304 2281 2394
Size: 295 1009 977 113
Fragments arranged by size:
1009 977 295 113
BseYI C'CCAG_C
Cuts at: 0 2026 2394
Size: 2026 368
BsiEI CG_ry'CG
Cuts at: 0 2323 2394
Size: 2323 71
BsiHKAI G_wGCw'C
Cuts at: 0 1696 2394
Size: 1696 698
BsmI GAATG_Cn'
Cuts at: 0 2164 2394
Size: 2164 230
Bsp1286I G_dGCh'C
Cuts at: 0 498 804 1532 1696 2394
Size: 498 306 728 164 698
Fragments arranged by size:
728 698 498 306 164
BspHI T'CATG_A
Cuts at: 0 1061 2394
Size: 1061 1333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 584 596 2394
Size: 584 12 1798
Fragments arranged by size:
1798 584 12
BsrFI r'CCGG_y
Cuts at: 0 1947 2394
Size: 1947 447
BsrGI T'GTAC_A
Cuts at: 0 1637 2394
Size: 1637 757
BssHII G'CGCG_C
Cuts at: 0 652 2394
Size: 652 1742
BssSI C'ACGA_G
Cuts at: 0 1135 2394
Size: 1135 1259
BstAPI GCAn_nnn'nTGC
Cuts at: 0 244 588 2394
Size: 244 344 1806
Fragments arranged by size:
1806 344 244
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 1671 2394
Size: 417 1254 723
Fragments arranged by size:
1254 723 417
BstYI r'GATC_y
Cuts at: 0 2059 2394
Size: 2059 335
BtgI C'CryG_G
Cuts at: 0 614 2340 2394
Size: 614 1726 54
Fragments arranged by size:
1726 614 54
BtsI GCAGTG_nn'
Cuts at: 0 107 1358 2055 2264 2394
Size: 107 1251 697 209 130
Fragments arranged by size:
1251 697 209 130 107
EaeI y'GGCC_r
Cuts at: 0 626 2394
Size: 626 1768
EarI CTCTTCn'nnn_
Cuts at: 0 1216 1466 2004 2134 2195 2394
Size: 1216 250 538 130 61 199
Fragments arranged by size:
1216 538 250 199 130 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 600 1876 2394
Size: 600 1276 518
Fragments arranged by size:
1276 600 518
EcoICRI GAG'CTC
Cuts at: 0 1694 2394
Size: 1694 700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 363 439 1206 1554 1778 2089 2300
Size: 92 271 76 767 348 224 311 211
Cuts at: 2300 2394
Size: 94
Fragments arranged by size:
767 348 311 271 224 211 94 92
76
EcoO109I rG'GnC_Cy
Cuts at: 0 494 495 693 1817 2366 2394
Size: 494 1 198 1124 549 28
Fragments arranged by size:
1124 549 494 198 28 1
EcoRV GAT'ATC
Cuts at: 0 170 2394
Size: 170 2224
FspI TGC'GCA
Cuts at: 0 993 2394
Size: 993 1401
HaeII r_GCGC'y
Cuts at: 0 325 2394
Size: 325 2069
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 12 44 343 375 1753 1785 1908 1940
Size: 12 32 299 32 1378 32 123 32
Cuts at: 1940 2044 2076 2260 2292 2305 2337 2394
Size: 104 32 184 32 13 32 57
Fragments arranged by size:
1378 299 184 123 104 57 32 32
32 32 32 32 32 13 12
HincII GTy'rAC
Cuts at: 0 573 783 2394
Size: 573 210 1611
Fragments arranged by size:
1611 573 210
KpnI G_GTAC'C
Cuts at: 0 1431 2394
Size: 1431 963
MfeI C'AATT_G
Cuts at: 0 899 2394
Size: 899 1495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1817 2394
Size: 1817 577
MslI CAynn'nnrTG
Cuts at: 0 313 715 814 1066 1105 1111 1669 2394
Size: 313 402 99 252 39 6 558 725
Fragments arranged by size:
725 558 402 313 252 99 39 6
MspA1I CmG'CkG
Cuts at: 0 2335 2394
Size: 2335 59
NaeI GCC'GGC
Cuts at: 0 1949 2394
Size: 1949 445
NcoI C'CATG_G
Cuts at: 0 2340 2394
Size: 2340 54
NgoMIV G'CCGG_C
Cuts at: 0 1947 2394
Size: 1947 447
NheI G'CTAG_C
Cuts at: 0 633 2394
Size: 633 1761
NsiI A_TGCA'T
Cuts at: 0 123 758 2394
Size: 123 635 1636
Fragments arranged by size:
1636 635 123
NspI r_CATG'y
Cuts at: 0 1800 2087 2394
Size: 1800 287 307
Fragments arranged by size:
1800 307 287
PasI CC'CwG_GG
Cuts at: 0 1937 2394
Size: 1937 457
PciI A'CATG_T
Cuts at: 0 1796 2394
Size: 1796 598
PflMI CCAn_nnn'nTGG
Cuts at: 0 476 2394
Size: 476 1918
PfoI T'CCnGG_A
Cuts at: 0 2055 2394
Size: 2055 339
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 2394
Size: 451 32 1911
Fragments arranged by size:
1911 451 32
PpuMI rG'GwC_Cy
Cuts at: 0 1817 2366 2394
Size: 1817 549 28
Fragments arranged by size:
1817 549 28
PspOMI G'GGCC_C
Cuts at: 0 494 2394
Size: 494 1900
PstI C_TGCA'G
Cuts at: 0 11 584 2007 2394
Size: 11 573 1423 387
Fragments arranged by size:
1423 573 387 11
PvuII CAG'CTG
Cuts at: 0 2335 2394
Size: 2335 59
SacI G_AGCT'C
Cuts at: 0 1696 2394
Size: 1696 698
SalI G'TCGA_C
Cuts at: 0 781 2394
Size: 781 1613
SapI GCTCTTCn'nnn_
Cuts at: 0 1466 2134 2394
Size: 1466 668 260
Fragments arranged by size:
1466 668 260
ScaI AGT'ACT
Cuts at: 0 1057 2206 2394
Size: 1057 1149 188
Fragments arranged by size:
1149 1057 188
SfcI C'TryA_G
Cuts at: 0 7 580 1275 1697 2003 2394
Size: 7 573 695 422 306 391
Fragments arranged by size:
695 573 422 391 306 7
SmlI C'TyrA_G
Cuts at: 0 61 1902 2232 2394
Size: 61 1841 330 162
Fragments arranged by size:
1841 330 162 61
SphI G_CATG'C
Cuts at: 0 2087 2394
Size: 2087 307
StuI AGG'CCT
Cuts at: 0 284 553 2302 2394
Size: 284 269 1749 92
Fragments arranged by size:
1749 284 269 92
StyI C'CwwG_G
Cuts at: 0 31 509 1339 2340 2394
Size: 31 478 830 1001 54
Fragments arranged by size:
1001 830 478 54 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 423 1099 2200 2309 2394
Size: 423 676 1101 109 85
Fragments arranged by size:
1101 676 423 109 85
TatI w'GTAC_w
Cuts at: 0 1055 1637 2204 2394
Size: 1055 582 567 190
Fragments arranged by size:
1055 582 567 190
Tth111I GACn'n_nGTC
Cuts at: 0 1821 2394
Size: 1821 573
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1671 2394
Size: 1671 723
XmnI GAAnn'nnTTC
Cuts at: 0 1302 1475 2164 2394
Size: 1302 173 689 230
Fragments arranged by size:
1302 689 230 173
ZraI GAC'GTC
Cuts at: 0 570 2394
Size: 570 1824
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AflIII AhdI AloI
AlwNI ApaI ApoI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI
BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRV FspI HaeII Hin4I HincII KpnI MfeI MmeI
MslI MspA1I NaeI NcoI NgoMIV NheI NsiI NspI
PasI PciI PflMI PfoI PpiI PpuMI PspOMI PstI
PvuII SacI SalI SapI ScaI SfcI SmlI SphI
StuI StyI TaqII TatI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AgeI AleI AlfI ApaLI AscI
AseI AsiSI AvaI BaeI BbeI BbvCI BglII BmgBI
BplI Bpu10I BsgI BsiWI BsmBI BspEI BsrBI BsrDI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoNI EcoRI FalI FseI FspAI
HindIII HpaI KasI MluI MscI NarI NdeI NotI
NruI PacI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TstI XbaI XhoI XmaI