(Linear) (Six-base) MAPSORT of: KIEE2684.seq  Check: 2948  from: 1  to: 651



KIEE2684.orf

 With 182 enzymes: SgfI * 

                        April 21, 2017 11:06  ..

AccI GT'mk_AC

Cuts at:      0     545     651 
   Size:        545     106
 
AleI CACnn'nnGTG

Cuts at:      0     402     651 
   Size:        402     249
 
BanII G_rGCy'C

Cuts at:      0     222     651 
   Size:        222     429
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     215     651 
   Size:        215     436
 
Bme1580I G_kGCm'C

Cuts at:      0     132     651 
   Size:        132     519
 
BsaWI w'CCGG_w

Cuts at:      0      15     651 
   Size:         15     636
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      15      45     651 
   Size:         15      30     606

  Fragments arranged by size: 

                 606      30      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     651 
   Size:         70     581
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     357     651 
   Size:        357     294
 
Bsp1286I G_dGCh'C

Cuts at:      0     132     222     651 
   Size:        132      90     429

  Fragments arranged by size: 

                 429     132      90
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     185     651 
   Size:        185     466
 
BsrBI CCG'CTC

Cuts at:      0     620     651 
   Size:        620      31
 
BsrGI T'GTAC_A

Cuts at:      0     224     651 
   Size:        224     427
 
BstZ17I GTA'TAC

Cuts at:      0     546     651 
   Size:        546     105
 
DraI TTT'AAA

Cuts at:      0     144     651 
   Size:        144     507
 
EarI CTCTTCn'nnn_

Cuts at:      0      47     651 
   Size:         47     604
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     179     651 
   Size:        179     472
 
HaeII r_GCGC'y

Cuts at:      0     455     651 
   Size:        455     196
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     116     148     573     605     651 
   Size:        116      32     425      32      46

  Fragments arranged by size: 

                 425     116      46      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     402     651 
   Size:        402     249
 
MspA1I CmG'CkG

Cuts at:      0     240     651 
   Size:        240     411
 
PvuII CAG'CTG

Cuts at:      0     240     651 
   Size:        240     411
 
SfcI C'TryA_G

Cuts at:      0      47     115     432     651 
   Size:         47      68     317     219

  Fragments arranged by size: 

                 317     219      68      47
 
TatI w'GTAC_w

Cuts at:      0     224     651 
   Size:        224     427
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      15      47     651 
   Size:         15      32     604

  Fragments arranged by size: 

                 604      32      15
 

 Enzymes that do cut:

     AccI     AleI    BanII     BbsI Bme1580I    BsaWI    BsaXI    BseRI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrGI  BstZ17I     DraI     EarI
     FalI    HaeII    Hin4I     MslI   MspA1I    PvuII     SfcI     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI