Restriction map for
pF1KE2690
(Linear) (Six-base) MAPSORT of: KIEE2690.seq Check: 8637 from: 1 to: 1629
KIEE2690.orf
With 182 enzymes: SgfI *
June 2, 2017 11:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1520 1629
Size: 1520 109
AhdI GACnn_n'nnGTC
Cuts at: 0 1542 1629
Size: 1542 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 464 1629
Size: 464 1165
AlwNI CAG_nnn'CTG
Cuts at: 0 68 813 1629
Size: 68 745 816
Fragments arranged by size:
816 745 68
AvaI C'yCGr_G
Cuts at: 0 774 1152 1360 1629
Size: 774 378 208 269
Fragments arranged by size:
774 378 269 208
AvrII C'CTAG_G
Cuts at: 0 894 1629
Size: 894 735
BamHI G'GATC_C
Cuts at: 0 351 707 1148 1629
Size: 351 356 441 481
Fragments arranged by size:
481 441 356 351
BanII G_rGCy'C
Cuts at: 0 884 1136 1562 1629
Size: 884 252 426 67
Fragments arranged by size:
884 426 252 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 87 1601 1629
Size: 87 1514 28
Fragments arranged by size:
1514 87 28
BglI GCCn_nnn'nGGC
Cuts at: 0 1057 1629
Size: 1057 572
BlpI GC'TnA_GC
Cuts at: 0 814 1629
Size: 814 815
Bme1580I G_kGCm'C
Cuts at: 0 464 1053 1629
Size: 464 589 576
Fragments arranged by size:
589 576 464
BmgBI CAC'GTC
Cuts at: 0 93 645 1014 1629
Size: 93 552 369 615
Fragments arranged by size:
615 552 369 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 333 413 1629
Size: 333 80 1216
Fragments arranged by size:
1216 333 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1428 1629
Size: 1428 201
Bpu10I CC'TnA_GC
Cuts at: 0 112 1629
Size: 112 1517
BsaI GGTCTCn'nnnn_
Cuts at: 0 682 1255 1629
Size: 682 573 374
Fragments arranged by size:
682 573 374
BsaAI yAC'GTr
Cuts at: 0 969 1596 1629
Size: 969 627 33
Fragments arranged by size:
969 627 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 679 709 1629
Size: 679 30 920
Fragments arranged by size:
920 679 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 700 748 1629
Size: 37 663 48 881
Fragments arranged by size:
881 663 48 37
BseYI C'CCAG_C
Cuts at: 0 84 328 342 457 756 904 910 916
Size: 84 244 14 115 299 148 6 6
Cuts at: 916 1031 1629
Size: 115 598
Fragments arranged by size:
598 299 244 148 115 115 84 14
6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 383 667 736 1044 1576 1629
Size: 304 79 284 69 308 532 53
Fragments arranged by size:
532 308 304 284 79 69 53
BsiEI CG_ry'CG
Cuts at: 0 143 1629
Size: 143 1486
BsiHKAI G_wGCw'C
Cuts at: 0 365 1136 1629
Size: 365 771 493
Fragments arranged by size:
771 493 365
Bsp1286I G_dGCh'C
Cuts at: 0 365 464 884 1053 1136 1562 1629
Size: 365 99 420 169 83 426 67
Fragments arranged by size:
426 420 365 169 99 83 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 326 1520 1547 1629
Size: 326 1194 27 82
Fragments arranged by size:
1194 326 82 27
BsrBI CCG'CTC
Cuts at: 0 296 1629
Size: 296 1333
BsrDI GCAATG_nn'
Cuts at: 0 627 1629
Size: 627 1002
BsrFI r'CCGG_y
Cuts at: 0 245 389 1629
Size: 245 144 1240
Fragments arranged by size:
1240 245 144
BsrGI T'GTAC_A
Cuts at: 0 998 1629
Size: 998 631
BssHII G'CGCG_C
Cuts at: 0 378 1168 1629
Size: 378 790 461
Fragments arranged by size:
790 461 378
BssSI C'ACGA_G
Cuts at: 0 1086 1629
Size: 1086 543
BstXI CCAn_nnnn'nTGG
Cuts at: 0 511 833 1629
Size: 511 322 796
Fragments arranged by size:
796 511 322
BstYI r'GATC_y
Cuts at: 0 351 707 719 1148 1364 1629
Size: 351 356 12 429 216 265
Fragments arranged by size:
429 356 351 265 216 12
Bsu36I CC'TnA_GG
Cuts at: 0 12 1629
Size: 12 1617
BtgI C'CryG_G
Cuts at: 0 143 498 1139 1265 1629
Size: 143 355 641 126 364
Fragments arranged by size:
641 364 355 143 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1194 1629
Size: 1194 435
BtsI GCAGTG_nn'
Cuts at: 0 286 1629
Size: 286 1343
ClaI AT'CG_AT
Cuts at: 0 749 1629
Size: 749 880
DraIII CAC_nnn'GTG
Cuts at: 0 272 1629
Size: 272 1357
EaeI y'GGCC_r
Cuts at: 0 34 140 158 501 1058 1629
Size: 34 106 18 343 557 571
Fragments arranged by size:
571 557 343 106 34 18
EagI C'GGCC_G
Cuts at: 0 140 1629
Size: 140 1489
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1486 1629
Size: 1486 143
EcoICRI GAG'CTC
Cuts at: 0 1134 1629
Size: 1134 495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1428 1629
Size: 1428 201
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 1381 1629
Size: 172 1209 248
Fragments arranged by size:
1209 248 172
EcoO109I rG'GnC_Cy
Cuts at: 0 1248 1401 1629
Size: 1248 153 228
Fragments arranged by size:
1248 228 153
FseI GG_CCGG'CC
Cuts at: 0 393 1629
Size: 393 1236
HaeII r_GCGC'y
Cuts at: 0 578 1629
Size: 578 1051
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 704 736 1385 1417 1528 1560 1629
Size: 704 32 649 32 111 32 69
Fragments arranged by size:
704 649 111 69 32 32 32
MscI TGG'CCA
Cuts at: 0 160 503 1629
Size: 160 343 1126
Fragments arranged by size:
1126 343 160
MslI CAynn'nnrTG
Cuts at: 0 52 544 1629
Size: 52 492 1085
Fragments arranged by size:
1085 492 52
MspA1I CmG'CkG
Cuts at: 0 139 756 813 1629
Size: 139 617 57 816
Fragments arranged by size:
816 617 139 57
NaeI GCC'GGC
Cuts at: 0 391 1629
Size: 391 1238
NcoI C'CATG_G
Cuts at: 0 1139 1629
Size: 1139 490
NgoMIV G'CCGG_C
Cuts at: 0 389 1629
Size: 389 1240
NspI r_CATG'y
Cuts at: 0 480 1005 1584 1629
Size: 480 525 579 45
Fragments arranged by size:
579 525 480 45
PasI CC'CwG_GG
Cuts at: 0 384 833 1629
Size: 384 449 796
Fragments arranged by size:
796 449 384
PfoI T'CCnGG_A
Cuts at: 0 1574 1629
Size: 1574 55
PpuMI rG'GwC_Cy
Cuts at: 0 1401 1629
Size: 1401 228
PvuII CAG'CTG
Cuts at: 0 756 813 1629
Size: 756 57 816
Fragments arranged by size:
816 756 57
SacI G_AGCT'C
Cuts at: 0 1136 1629
Size: 1136 493
SexAI A'CCwGG_T
Cuts at: 0 173 1629
Size: 173 1456
SfcI C'TryA_G
Cuts at: 0 198 1629
Size: 198 1431
SmaI CCC'GGG
Cuts at: 0 776 1629
Size: 776 853
SmlI C'TyrA_G
Cuts at: 0 1360 1629
Size: 1360 269
StuI AGG'CCT
Cuts at: 0 1204 1282 1629
Size: 1204 78 347
Fragments arranged by size:
1204 347 78
StyI C'CwwG_G
Cuts at: 0 894 1127 1139 1629
Size: 894 233 12 490
Fragments arranged by size:
894 490 233 12
TatI w'GTAC_w
Cuts at: 0 998 1037 1629
Size: 998 39 592
Fragments arranged by size:
998 592 39
Tth111I GACn'n_nGTC
Cuts at: 0 1399 1629
Size: 1399 230
XbaI T'CTAG_A
Cuts at: 0 1533 1629
Size: 1533 96
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 432 1629
Size: 432 1197
XhoI C'TCGA_G
Cuts at: 0 1360 1629
Size: 1360 269
XmaI C'CCGG_G
Cuts at: 0 774 1629
Size: 774 855
Enzymes that do cut:
AarI AhdI AlfI AlwNI AvaI AvrII BamHI BanII
BbsI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI
HaeII Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PfoI PpuMI PvuII SacI SexAI SfcI
SmaI SmlI StuI StyI TatI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI BaeI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BmtI BplI BpuEI BsaBI BsaHI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BstAPI BstBI BstEII
BstZ17I CspCI DraI DrdI EarI EcoRI EcoRV FalI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NdeI NheI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI
XmnI ZraI