Restriction map for
pF1KE2695
(Linear) (Six-base) MAPSORT of: KIEE2695.seq Check: 6513 from: 1 to: 7017
KIEE2695.orf
With 182 enzymes: SgfI *
July 6, 2017 13:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 618 2420 4845 7017
Size: 618 1802 2425 2172
Fragments arranged by size:
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AccI GT'mk_AC
Cuts at: 0 2534 3863 4193 7017
Size: 2534 1329 330 2824
Fragments arranged by size:
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Acc65I G'GTAC_C
Cuts at: 0 1160 4160 5520 7017
Size: 1160 3000 1360 1497
Fragments arranged by size:
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AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 619 1138 1267 1612 2246 3856 4579
Size: 216 403 519 129 345 634 1610 723
Cuts at: 4579 5698 7017
Size: 1119 1319
Fragments arranged by size:
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216 129
AfeI AGC'GCT
Cuts at: 0 6058 6376 7017
Size: 6058 318 641
Fragments arranged by size:
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AflIII A'CryG_T
Cuts at: 0 4802 5243 5264 6887 7017
Size: 4802 441 21 1623 130
Fragments arranged by size:
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AgeI A'CCGG_T
Cuts at: 0 1307 7017
Size: 1307 5710
AhdI GACnn_n'nnGTC
Cuts at: 0 3110 7017
Size: 3110 3907
AleI CACnn'nnGTG
Cuts at: 0 4456 7017
Size: 4456 2561
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5039 5353 7017
Size: 5039 314 1664
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1393 1425 7017
Size: 1393 32 5592
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 1977 2337 3137 6741 6789 7017
Size: 1977 360 800 3604 48 228
Fragments arranged by size:
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ApaI G_GGCC'C
Cuts at: 0 43 63 94 1613 1737 2486 2696 2897
Size: 43 20 31 1519 124 749 210 201
Cuts at: 2897 2943 3259 5953 6212 7017
Size: 46 316 2694 259 805
Fragments arranged by size:
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124 46 43 31 20
AvaI C'yCGr_G
Cuts at: 0 62 93 100 1065 2377 2524 2642 2734
Size: 62 31 7 965 1312 147 118 92
Cuts at: 2734 2885 2897 3065 5303 5723 6270 6861 6963
Size: 151 12 168 2238 420 547 591 102
Cuts at: 6963 7017
Size: 54
Fragments arranged by size:
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147 118 102 92 62 54 31 12
7
AvrII C'CTAG_G
Cuts at: 0 5160 7017
Size: 5160 1857
BamHI G'GATC_C
Cuts at: 0 530 1333 2823 3712 7017
Size: 530 803 1490 889 3305
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 1160 2549 2558 2638 2750 2816 2845 2870
Size: 1160 1389 9 80 112 66 29 25
Cuts at: 2870 2933 3685 4160 5520 7017
Size: 63 752 475 1360 1497
Fragments arranged by size:
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66 63 29 25 9
BanII G_rGCy'C
Cuts at: 0 43 63 94 404 478 785 1154 1381
Size: 43 20 31 310 74 307 369 227
Cuts at: 1381 1613 1737 1780 2486 2636 2696 2795 2897
Size: 232 124 43 706 150 60 99 102
Cuts at: 2897 2943 3050 3259 4062 5724 5953 5966 6212
Size: 46 107 209 803 1662 229 13 246
Cuts at: 6212 6413 6514 6648 7017
Size: 201 101 134 369
Fragments arranged by size:
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232 229 227 209 201 150 134 124
107 102 101 99 74 60 46 43
43 31 20 13
BbeI G_GCGC'C
Cuts at: 0 2849 3689 7017
Size: 2849 840 3328
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 222 456 1375 1857 1959 2434 5509 7017
Size: 222 234 919 482 102 475 3075 1508
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 589 1747 3506 4690 5758 6433 6730 7017
Size: 589 1158 1759 1184 1068 675 297 287
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4305 4339 5197 5231 7017
Size: 4305 34 858 34 1786
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 4241 4724 5117 7017
Size: 4241 483 393 1900
Fragments arranged by size:
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BglI GCCn_nnn'nGGC
Cuts at: 0 47 82 1524 2471 2617 2738 2928 3694
Size: 47 35 1442 947 146 121 190 766
Cuts at: 3694 5280 7017
Size: 1586 1737
Fragments arranged by size:
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47 35
BglII A'GATC_T
Cuts at: 0 1317 7017
Size: 1317 5700
BlpI GC'TnA_GC
Cuts at: 0 4007 6682 7017
Size: 4007 2675 335
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 43 63 94 1613 1737 2486 2643 2696
Size: 43 20 31 1519 124 749 157 53
Cuts at: 2696 2897 2943 3259 5821 5953 6212 6932 7017
Size: 201 46 316 2562 132 259 720 85
Fragments arranged by size:
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132 124 85 53 46 43 31 20
BmgBI CAC'GTC
Cuts at: 0 1242 3345 4391 5375 7017
Size: 1242 2103 1046 984 1642
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 1464 3235 3278 3508 6900 7017
Size: 1464 1771 43 230 3392 117
Fragments arranged by size:
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BmtI G_CTAG'C
Cuts at: 0 6539 7017
Size: 6539 478
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5826 6745 7017
Size: 5826 919 272
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 745 1186 1794 3106 3127 3653 3973
Size: 361 384 441 608 1312 21 526 320
Cuts at: 3973 4315 5428 5654 5710 5884 5998 6220 6858
Size: 342 1113 226 56 174 114 222 638
Cuts at: 6858 7017
Size: 159
Fragments arranged by size:
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21
Bpu10I CC'TnA_GC
Cuts at: 0 589 1747 3506 4690 4993 5758 6433 6730
Size: 589 1158 1759 1184 303 765 675 297
Cuts at: 6730 7017
Size: 287
Fragments arranged by size:
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287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1342 3504 3760 3787 7017
Size: 622 720 2162 256 27 3230
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 1573 2977 3082 4410 5606 6716 7017
Size: 1573 1404 105 1328 1196 1110 301
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 4188 4803 6888 7017
Size: 4188 615 2085 129
Fragments arranged by size:
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BsaBI GATnn'nnATC
Cuts at: 0 442 4246 7017
Size: 442 3804 2771
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 2033 2531 2799 2846 3686 6139 6577 7017
Size: 2033 498 268 47 840 2453 438 440
Fragments arranged by size:
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BsaWI w'CCGG_w
Cuts at: 0 1307 1391 1412 1625 3050 4913 5396 7017
Size: 1307 84 21 213 1425 1863 483 1621
Fragments arranged by size:
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BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1393 1423 1762 1792 5050 5080 5711 5741
Size: 1393 30 339 30 3258 30 631 30
Cuts at: 5741 6877 6907 7017
Size: 1136 30 110
Fragments arranged by size:
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30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1219 1237 1309 2182 2395 2662 2788 2932
Size: 1219 18 72 873 213 267 126 144
Cuts at: 2932 3366 4153 5732 6348 6351 6612 6856 6861
Size: 434 787 1579 616 3 261 244 5
Cuts at: 6861 7017
Size: 156
Fragments arranged by size:
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244 213 156 144 126 72 18 5
3
BseYI C'CCAG_C
Cuts at: 0 21 816 1053 1875 2460 3130 5283 5424
Size: 21 795 237 822 585 670 2153 141
Cuts at: 5424 5428 5966 6077 6211 6595 7017
Size: 4 538 111 134 384 422
Fragments arranged by size:
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237 141 134 111 21 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3915 3922 6930 7017
Size: 3915 7 3008 87
Fragments arranged by size:
3915 3008 87 7
BsiEI CG_ry'CG
Cuts at: 0 30 146 980 2446 2656 2662 2721 2745
Size: 30 116 834 1466 210 6 59 24
Cuts at: 2745 2783 3278 6020 6393 6549 7017
Size: 38 495 2742 373 156 468
Fragments arranged by size:
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116 59 38 30 24 6
BsiHKAI G_wGCw'C
Cuts at: 0 638 1064 1154 1381 1780 3050 4055 5086
Size: 638 426 90 227 399 1270 1005 1031
Cuts at: 5086 5714 5910 6978 7017
Size: 628 196 1068 39
Fragments arranged by size:
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227 196 90 39
BsmI GAATG_Cn'
Cuts at: 0 1221 2001 4291 4555 7017
Size: 1221 780 2290 264 2462
Fragments arranged by size:
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BsmBI CGTCTCn'nnnn_
Cuts at: 0 5070 7017
Size: 5070 1947
Bsp1286I G_dGCh'C
Cuts at: 0 43 63 94 404 478 638 785 1064
Size: 43 20 31 310 74 160 147 279
Cuts at: 1064 1154 1381 1613 1737 1780 2486 2636 2643
Size: 90 227 232 124 43 706 150 7
Cuts at: 2643 2696 2795 2897 2943 3050 3259 4055 4062
Size: 53 99 102 46 107 209 796 7
Cuts at: 4062 5086 5714 5724 5821 5910 5953 5966 6212
Size: 1024 628 10 97 89 43 13 246
Cuts at: 6212 6413 6514 6648 6932 6978 7017
Size: 201 101 134 284 46 39
Fragments arranged by size:
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232 227 209 201 160 150 147 134
124 107 102 101 99 97 90 89
74 53 46 46 43 43 43 39
31 20 13 10 7 7
BspEI T'CCGG_A
Cuts at: 0 1391 3050 4913 5396 7017
Size: 1391 1659 1863 483 1621
Fragments arranged by size:
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BspHI T'CATG_A
Cuts at: 0 647 7017
Size: 647 6370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 491 569 618 2345 2420 3419 4845 7017
Size: 491 78 49 1727 75 999 1426 2172
Fragments arranged by size:
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BsrBI CCG'CTC
Cuts at: 0 57 218 384 1255 1364 2385 2627 2652
Size: 57 161 166 871 109 1021 242 25
Cuts at: 2652 2787 2859 2890 2912 3356 5995 6321 6339
Size: 135 72 31 22 444 2639 326 18
Cuts at: 6339 6869 7017
Size: 530 148
Fragments arranged by size:
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161 148 135 109 72 57 31 25
22 18
BsrDI GCAATG_nn'
Cuts at: 0 3893 4814 5769 7017
Size: 3893 921 955 1248
Fragments arranged by size:
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BsrFI r'CCGG_y
Cuts at: 0 76 979 1127 1307 2441 2555 2744 2756
Size: 76 903 148 180 1134 114 189 12
Cuts at: 2756 2813 2842 2922 4367 5703 6293 6311 6326
Size: 57 29 80 1445 1336 590 18 15
Cuts at: 6326 6677 7017
Size: 351 340
Fragments arranged by size:
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180 148 114 80 76 57 29 18
15 12
BsrGI T'GTAC_A
Cuts at: 0 173 2360 3231 7017
Size: 173 2187 871 3786
Fragments arranged by size:
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BssHII G'CGCG_C
Cuts at: 0 153 155 2290 2474 2807 2862 2864 7017
Size: 153 2 2135 184 333 55 2 4153
Fragments arranged by size:
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BssSI C'ACGA_G
Cuts at: 0 2965 3469 4972 7017
Size: 2965 504 1503 2045
Fragments arranged by size:
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BstAPI GCAn_nnn'nTGC
Cuts at: 0 633 2337 2837 3126 7017
Size: 633 1704 500 289 3891
Fragments arranged by size:
3891 1704 633 500 289
BstEII G'GTnAC_C
Cuts at: 0 6937 7017
Size: 6937 80
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2124 2220 3951 5385 5791 7017
Size: 2124 96 1731 1434 406 1226
Fragments arranged by size:
2124 1731 1434 1226 406 96
BstYI r'GATC_y
Cuts at: 0 416 530 1172 1317 1333 1751 1973 2823
Size: 416 114 642 145 16 418 222 850
Cuts at: 2823 3712 5885 7017
Size: 889 2173 1132
Fragments arranged by size:
2173 1132 889 850 642 418 416 222
145 114 16
Bsu36I CC'TnA_GG
Cuts at: 0 2507 6821 7017
Size: 2507 4314 196
Fragments arranged by size:
4314 2507 196
BtgI C'CryG_G
Cuts at: 0 164 539 656 785 935 1499 1519 2486
Size: 164 375 117 129 150 564 20 967
Cuts at: 2486 2582 2656 2687 2874 2987 3002 3272 4085
Size: 96 74 31 187 113 15 270 813
Cuts at: 4085 4103 4121 4544 4955 6087 6549 6687 7017
Size: 18 18 423 411 1132 462 138 330
Fragments arranged by size:
1132 967 813 564 462 423 411 375
330 270 187 164 150 138 129 117
113 96 74 31 20 18 18 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 300 432 2103 2244 3462 3477 4551 7017
Size: 300 132 1671 141 1218 15 1074 2466
Fragments arranged by size:
2466 1671 1218 1074 300 141 132 15
BtsI GCAGTG_nn'
Cuts at: 0 2006 3124 4024 6252 6498 6942 7017
Size: 2006 1118 900 2228 246 444 75
Fragments arranged by size:
2228 2006 1118 900 444 246 75
ClaI AT'CG_AT
Cuts at: 0 142 7017
Size: 142 6875
DraIII CAC_nnn'GTG
Cuts at: 0 278 845 1490 1492 3079 4122 5033 7017
Size: 278 567 645 2 1587 1043 911 1984
Fragments arranged by size:
1984 1587 1043 911 645 567 278 2
DrdI GACnn_nn'nnGTC
Cuts at: 0 247 514 1654 6906 7017
Size: 247 267 1140 5252 111
Fragments arranged by size:
5252 1140 267 247 111
EaeI y'GGCC_r
Cuts at: 0 27 117 305 859 977 1199 1256 1496
Size: 27 90 188 554 118 222 57 240
Cuts at: 1496 1691 2114 2135 2282 2443 2653 2659 2718
Size: 195 423 21 147 161 210 6 59
Cuts at: 2718 2742 2780 4433 5871 6017 6462 6546 6614
Size: 24 38 1653 1438 146 445 84 68
Cuts at: 6614 6851 6969 7017
Size: 237 118 48
Fragments arranged by size:
1653 1438 554 445 423 240 237 222
210 195 188 161 147 146 118 118
90 84 68 59 57 48 38 27
24 21 6
EagI C'GGCC_G
Cuts at: 0 27 977 2443 2653 2659 2718 2742 2780
Size: 27 950 1466 210 6 59 24 38
Cuts at: 2780 6017 6546 7017
Size: 3237 529 471
Fragments arranged by size:
3237 1466 950 529 471 210 59 38
27 24 6
EarI CTCTTCn'nnn_
Cuts at: 0 229 694 1243 1261 1576 1843 1852 2159
Size: 229 465 549 18 315 267 9 307
Cuts at: 2159 3877 3913 5157 5737 7017
Size: 1718 36 1244 580 1280
Fragments arranged by size:
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267 229 36 18 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 65 1198 1729 2635 2920 3406 4690 4726
Size: 65 1133 531 906 285 486 1284 36
Cuts at: 4726 6573 6910 7017
Size: 1847 337 107
Fragments arranged by size:
1847 1284 1133 906 531 486 337 285
107 65 36
EcoICRI GAG'CTC
Cuts at: 0 1152 1379 1778 3048 7017
Size: 1152 227 399 1270 3969
Fragments arranged by size:
3969 1270 1152 399 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 361 619 745 1138 1186 1267 1612
Size: 216 145 258 126 393 48 81 345
Cuts at: 1612 1794 2246 3106 3127 3653 3856 3973 4315
Size: 182 452 860 21 526 203 117 342
Cuts at: 4315 4579 5428 5654 5698 5710 5884 5998 6220
Size: 264 849 226 44 12 174 114 222
Cuts at: 6220 6858 7017
Size: 638 159
Fragments arranged by size:
860 849 638 526 452 393 345 342
264 258 226 222 216 203 182 174
159 145 126 117 114 81 48 44
21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 6202 6820 7017
Size: 6202 618 197
Fragments arranged by size:
6202 618 197
EcoO109I rG'GnC_Cy
Cuts at: 0 39 40 90 97 126 593 813 865
Size: 39 1 50 7 29 467 220 52
Cuts at: 865 1609 1734 2482 2520 2610 2692 2693 2731
Size: 744 125 748 38 90 82 1 38
Cuts at: 2731 2893 2894 3112 3256 3510 4791 5164 5653
Size: 162 1 218 144 254 1281 373 489
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Size: 175 121 1 244 14 72 195 542
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244 220 218 195 175 162 144 125
121 90 82 72 52 50 39 38
38 29 14 7 1 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 6393 7017
Size: 6393 624
FspI TGC'GCA
Cuts at: 0 3520 7017
Size: 3520 3497
FspAI rTGC'GCAy
Cuts at: 0 3520 7017
Size: 3520 3497
HaeII r_GCGC'y
Cuts at: 0 172 2359 2849 3689 4337 4955 5322 6060
Size: 172 2187 490 840 648 618 367 738
Cuts at: 6060 6312 6378 7017
Size: 252 66 639
Fragments arranged by size:
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252 172 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1213 1245 1366 1398 3096 3128 3243 3275
Size: 1213 32 121 32 1698 32 115 32
Cuts at: 3275 5973 6005 7017
Size: 2698 32 1012
Fragments arranged by size:
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32 32 32
HincII GTy'rAC
Cuts at: 0 213 2535 4224 6524 7017
Size: 213 2322 1689 2300 493
Fragments arranged by size:
2322 2300 1689 493 213
HindIII A'AGCT_T
Cuts at: 0 2194 7017
Size: 2194 4823
KasI G'GCGC_C
Cuts at: 0 2845 3685 7017
Size: 2845 840 3332
Fragments arranged by size:
3332 2845 840
KpnI G_GTAC'C
Cuts at: 0 1164 4164 5524 7017
Size: 1164 3000 1360 1493
Fragments arranged by size:
3000 1493 1360 1164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1311 5372 7017
Size: 1311 4061 1645
Fragments arranged by size:
4061 1645 1311
MscI TGG'CCA
Cuts at: 0 307 861 1498 2116 2137 4435 5873 6464
Size: 307 554 637 618 21 2298 1438 591
Cuts at: 6464 6853 7017
Size: 389 164
Fragments arranged by size:
2298 1438 637 618 591 554 389 307
164 21
MslI CAynn'nnrTG
Cuts at: 0 703 934 1582 2122 3196 3469 3898 4231
Size: 703 231 648 540 1074 273 429 333
Cuts at: 4231 4435 4456 4534 4819 4849 4855 5177 5335
Size: 204 21 78 285 30 6 322 158
Cuts at: 5335 5383 5447 7017
Size: 48 64 1570
Fragments arranged by size:
1570 1074 703 648 540 429 333 322
285 273 231 204 158 78 64 48
30 21 6
MspA1I CmG'CkG
Cuts at: 0 123 1521 1887 2310 2348 2448 2584 2658
Size: 123 1398 366 423 38 100 136 74
Cuts at: 2658 2689 2876 3183 5428 6081 6164 6460 6551
Size: 31 187 307 2245 653 83 296 91
Cuts at: 6551 7017
Size: 466
Fragments arranged by size:
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187 136 123 100 91 83 74 38
31
NaeI GCC'GGC
Cuts at: 0 78 981 1129 2746 2844 2924 5705 6295
Size: 78 903 148 1617 98 80 2781 590
Cuts at: 6295 6313 6679 7017
Size: 18 366 338
Fragments arranged by size:
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80 78 18
NarI GG'CG_CC
Cuts at: 0 2846 3686 7017
Size: 2846 840 3331
Fragments arranged by size:
3331 2846 840
NcoI C'CATG_G
Cuts at: 0 164 656 935 1499 4103 4544 7017
Size: 164 492 279 564 2604 441 2473
Fragments arranged by size:
2604 2473 564 492 441 279 164
NgoMIV G'CCGG_C
Cuts at: 0 76 979 1127 2744 2842 2922 5703 6293
Size: 76 903 148 1617 98 80 2781 590
Cuts at: 6293 6311 6677 7017
Size: 18 366 340
Fragments arranged by size:
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80 76 18
NheI G'CTAG_C
Cuts at: 0 6535 7017
Size: 6535 482
NotI GC'GGCC_GC
Cuts at: 0 27 2653 2659 7017
Size: 27 2626 6 4358
Fragments arranged by size:
4358 2626 27 6
NspI r_CATG'y
Cuts at: 0 2049 3870 4359 4854 5247 5268 5340 7017
Size: 2049 1821 489 495 393 21 72 1677
Fragments arranged by size:
2049 1821 1677 495 489 393 72 21
PasI CC'CwG_GG
Cuts at: 0 4156 6951 7017
Size: 4156 2795 66
Fragments arranged by size:
4156 2795 66
PciI A'CATG_T
Cuts at: 0 5243 5264 7017
Size: 5243 21 1753
Fragments arranged by size:
5243 1753 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 3150 4019 4156 6460 6741 7017
Size: 3150 869 137 2304 281 276
Fragments arranged by size:
3150 2304 869 281 276 137
PfoI T'CCnGG_A
Cuts at: 0 3644 4256 5631 6874 7017
Size: 3644 612 1375 1243 143
Fragments arranged by size:
3644 1375 1243 612 143
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1393 1425 7017
Size: 1393 32 5592
Fragments arranged by size:
5592 1393 32
PpuMI rG'GwC_Cy
Cuts at: 0 97 126 865 3112 3510 5164 5653 5828
Size: 97 29 739 2247 398 1654 489 175
Cuts at: 5828 6194 6475 7017
Size: 366 281 542
Fragments arranged by size:
2247 1654 739 542 489 398 366 281
175 97 29
PshAI GACnn'nnGTC
Cuts at: 0 4774 7017
Size: 4774 2243
PspOMI G'GGCC_C
Cuts at: 0 39 59 90 1609 1733 2482 2692 2893
Size: 39 20 31 1519 124 749 210 201
Cuts at: 2893 2939 3255 5949 6208 7017
Size: 46 316 2694 259 809
Fragments arranged by size:
2694 1519 809 749 316 259 210 201
124 46 39 31 20
PspXI vC'TCGA_Gb
Cuts at: 0 5303 7017
Size: 5303 1714
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 985 1017 7017
Size: 985 32 6000
Fragments arranged by size:
6000 985 32
PstI C_TGCA'G
Cuts at: 0 113 674 2333 3967 6719 7017
Size: 113 561 1659 1634 2752 298
Fragments arranged by size:
2752 1659 1634 561 298 113
PvuI CG_AT'CG
Cuts at: 0 146 7017
Size: 146 6871
PvuII CAG'CTG
Cuts at: 0 1887 2348 5428 6460 7017
Size: 1887 461 3080 1032 557
Fragments arranged by size:
3080 1887 1032 557 461
RsrII CG'GwC_CG
Cuts at: 0 6117 7017
Size: 6117 900
SacI G_AGCT'C
Cuts at: 0 1154 1381 1780 3050 7017
Size: 1154 227 399 1270 3967
Fragments arranged by size:
3967 1270 1154 399 227
SacII CC_GC'GG
Cuts at: 0 1522 2585 2659 2690 2877 6552 7017
Size: 1522 1063 74 31 187 3675 465
Fragments arranged by size:
3675 1522 1063 465 187 74 31
SalI G'TCGA_C
Cuts at: 0 2533 7017
Size: 2533 4484
SanDI GG'GwC_CC
Cuts at: 0 97 3112 6475 7017
Size: 97 3015 3363 542
Fragments arranged by size:
3363 3015 542 97
SapI GCTCTTCn'nnn_
Cuts at: 0 229 1261 1843 1852 3877 3913 7017
Size: 229 1032 582 9 2025 36 3104
Fragments arranged by size:
3104 2025 1032 582 229 36 9
ScaI AGT'ACT
Cuts at: 0 856 1771 7017
Size: 856 915 5246
Fragments arranged by size:
5246 915 856
SexAI A'CCwGG_T
Cuts at: 0 1046 1790 7017
Size: 1046 744 5227
Fragments arranged by size:
5227 1046 744
SfcI C'TryA_G
Cuts at: 0 109 670 2329 3963 6284 6715 7017
Size: 109 561 1659 1634 2321 431 302
Fragments arranged by size:
2321 1659 1634 561 431 302 109
SfoI GGC'GCC
Cuts at: 0 2847 3687 7017
Size: 2847 840 3330
Fragments arranged by size:
3330 2847 840
SgrAI Cr'CCGG_yG
Cuts at: 0 2756 2842 7017
Size: 2756 86 4175
Fragments arranged by size:
4175 2756 86
SmaI CCC'GGG
Cuts at: 0 64 95 102 2644 2899 3067 5725 6272
Size: 64 31 7 2542 255 168 2658 547
Cuts at: 6272 7017
Size: 745
Fragments arranged by size:
2658 2542 745 547 255 168 64 31
7
SmlI C'TyrA_G
Cuts at: 0 637 1357 3483 3775 3802 5303 7017
Size: 637 720 2126 292 27 1501 1714
Fragments arranged by size:
2126 1714 1501 720 637 292 27
SphI G_CATG'C
Cuts at: 0 4854 7017
Size: 4854 2163
SspI AAT'ATT
Cuts at: 0 4869 7017
Size: 4869 2148
StuI AGG'CCT
Cuts at: 0 754 4966 5005 5639 6802 7017
Size: 754 4212 39 634 1163 215
Fragments arranged by size:
4212 1163 754 634 215 39
StyI C'CwwG_G
Cuts at: 0 164 656 935 1079 1499 2156 2285 2849
Size: 164 492 279 144 420 657 129 564
Cuts at: 2849 3944 4103 4544 5160 7017
Size: 1095 159 441 616 1857
Fragments arranged by size:
1857 1095 657 616 564 492 441 420
279 164 159 144 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 778 1446 2615 6458 6497 6642 7017
Size: 778 668 1169 3843 39 145 375
Fragments arranged by size:
3843 1169 778 668 375 145 39
TatI w'GTAC_w
Cuts at: 0 173 854 1769 2360 3231 7017
Size: 173 681 915 591 871 3786
Fragments arranged by size:
3786 915 871 681 591 173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3264 3296 5359 5391 7017
Size: 3264 32 2063 32 1626
Fragments arranged by size:
3264 2063 1626 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 244 4618 7017
Size: 244 4374 2399
Fragments arranged by size:
4374 2399 244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 720 3291 4185 4542 5859 6069 6085 7017
Size: 720 2571 894 357 1317 210 16 932
Fragments arranged by size:
2571 1317 932 894 720 357 210 16
XhoI C'TCGA_G
Cuts at: 0 5303 7017
Size: 5303 1714
XmaI C'CCGG_G
Cuts at: 0 62 93 100 2642 2897 3065 5723 6270
Size: 62 31 7 2542 255 168 2658 547
Cuts at: 6270 7017
Size: 747
Fragments arranged by size:
2658 2542 747 547 255 168 62 31
7
XmnI GAAnn'nnTTC
Cuts at: 0 202 1408 3922 4588 4678 7017
Size: 202 1206 2514 666 90 2339
Fragments arranged by size:
2514 2339 1206 666 202 90
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SfcI SfoI SgrAI
SmaI SmlI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII ApaLI ApoI AscI AseI AsiSI
BaeI BciVI BfrBI BsiWI BstBI BstZ17I CspCI DraI
EcoRI EcoRV FseI HpaI MfeI MluI NdeI NruI
NsiI PacI PmeI PmlI PsiI SbfI SfiI SgfI
SnaBI SpeI SrfI SwaI XbaI ZraI