Restriction map for pF1KE2721
 (Linear) (Six-base) MAPSORT of: KIEE2721.seq  Check: 8809  from: 1  to: 591



KIEE2721.orf

 With 182 enzymes: SgfI * 

                        June 6, 2018 17:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82      84     562     591 
   Size:         82       2     478      29

  Fragments arranged by size: 

                 478      82      29       2
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     310     591 
   Size:        310     281
 
AlwNI CAG_nnn'CTG

Cuts at:      0     399     591 
   Size:        399     192
 
ApaI G_GGCC'C

Cuts at:      0      10     591 
   Size:         10     581
 
AvaI C'yCGr_G

Cuts at:      0     126     228     591 
   Size:        126     102     363

  Fragments arranged by size: 

                 363     126     102
 
BanI G'GyrC_C

Cuts at:      0     586     591 
   Size:        586       5
 
BanII G_rGCy'C

Cuts at:      0      10     485     591 
   Size:         10     475     106

  Fragments arranged by size: 

                 475     106      10
 
BbeI G_GCGC'C

Cuts at:      0     590     591 
   Size:        590       1
 
BglI GCCn_nnn'nGGC

Cuts at:      0     274     591 
   Size:        274     317
 
BlpI GC'TnA_GC

Cuts at:      0      25     591 
   Size:         25     566
 
Bme1580I G_kGCm'C

Cuts at:      0      10     591 
   Size:         10     581
 
BmgBI CAC'GTC

Cuts at:      0     303     591 
   Size:        303     288
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     570     591 
   Size:        570      21
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     409     591 
   Size:         58     351     182

  Fragments arranged by size: 

                 351     182      58
 
BsaAI yAC'GTr

Cuts at:      0     384     591 
   Size:        384     207
 
BsaHI Gr'CG_yC

Cuts at:      0     587     591 
   Size:        587       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     154     439     591 
   Size:        154     285     152

  Fragments arranged by size: 

                 285     154     152
 
BseYI C'CCAG_C

Cuts at:      0      43     591 
   Size:         43     548
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     292     364     385     591 
   Size:        283       9      72      21     206

  Fragments arranged by size: 

                 283     206      72      21       9
 
BsiHKAI G_wGCw'C

Cuts at:      0      55      83     591 
   Size:         55      28     508

  Fragments arranged by size: 

                 508      55      28
 
Bsp1286I G_dGCh'C

Cuts at:      0      10      55      83     485     591 
   Size:         10      45      28     402     106

  Fragments arranged by size: 

                 402     106      45      28      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     317     591 
   Size:        317     274
 
BsrBI CCG'CTC

Cuts at:      0      29     591 
   Size:         29     562
 
BsrFI r'CCGG_y

Cuts at:      0     199     583     591 
   Size:        199     384       8

  Fragments arranged by size: 

                 384     199       8
 
BssSI C'ACGA_G

Cuts at:      0     117     246     591 
   Size:        117     129     345

  Fragments arranged by size: 

                 345     129     117
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     270     318     399     591 
   Size:        270      48      81     192

  Fragments arranged by size: 

                 270     192      81      48
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      18     591 
   Size:         18     573
 
BtsI GCAGTG_nn'

Cuts at:      0     376     591 
   Size:        376     215
 
EarI CTCTTCn'nnn_

Cuts at:      0      43      58     591 
   Size:         43      15     533

  Fragments arranged by size: 

                 533      43      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58      82      84     409     562     591 
   Size:         58      24       2     325     153      29

  Fragments arranged by size: 

                 325     153      58      29      24       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     523     591 
   Size:        523      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0       7     591 
   Size:          7     584
 
HaeII r_GCGC'y

Cuts at:      0     553     590     591 
   Size:        553      37       1

  Fragments arranged by size: 

                 553      37       1
 
KasI G'GCGC_C

Cuts at:      0     586     591 
   Size:        586       5
 
MslI CAynn'nnrTG

Cuts at:      0     292     361     591 
   Size:        292      69     230

  Fragments arranged by size: 

                 292     230      69
 
MspA1I CmG'CkG

Cuts at:      0      86     396     591 
   Size:         86     310     195

  Fragments arranged by size: 

                 310     195      86
 
NaeI GCC'GGC

Cuts at:      0     201     591 
   Size:        201     390
 
NarI GG'CG_CC

Cuts at:      0     587     591 
   Size:        587       4
 
NgoMIV G'CCGG_C

Cuts at:      0     199     591 
   Size:        199     392
 
NspI r_CATG'y

Cuts at:      0     220     591 
   Size:        220     371
 
PfoI T'CCnGG_A

Cuts at:      0      74     591 
   Size:         74     517
 
PmlI CAC'GTG

Cuts at:      0     384     591 
   Size:        384     207
 
PspOMI G'GGCC_C

Cuts at:      0       6     591 
   Size:          6     585
 
PstI C_TGCA'G

Cuts at:      0     314     591 
   Size:        314     277
 
SapI GCTCTTCn'nnn_

Cuts at:      0      43     591 
   Size:         43     548
 
SbfI CC_TGCA'GG

Cuts at:      0     314     591 
   Size:        314     277
 
SfcI C'TryA_G

Cuts at:      0     310     591 
   Size:        310     281
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     274     591 
   Size:        274     317
 
SfoI GGC'GCC

Cuts at:      0     588     591 
   Size:        588       3
 
SphI G_CATG'C

Cuts at:      0     220     591 
   Size:        220     371
 
StuI AGG'CCT

Cuts at:      0      15     529     591 
   Size:         15     514      62

  Fragments arranged by size: 

                 514      62      15
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI
     BglI     BlpI Bme1580I    BmgBI     BplI     BpmI    BsaAI    BsaHI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI
    BssSI   BstAPI    BstXI     BtsI     EarI  Eco57MI    EcoNI EcoO109I
    HaeII     KasI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PfoI     PmlI   PspOMI     PstI     SapI     SbfI     SfcI     SfiI
     SfoI     SphI     StuI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BmrI     BmtI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrDI    BsrGI   BssHII    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     ScaI    SexAI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com