(Linear) (Six-base) MAPSORT of: KIEE2721.seq Check: 8809 from: 1 to: 591 KIEE2721.orf With 182 enzymes: SgfI * June 6, 2018 17:50 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 84 562 591 Size: 82 2 478 29 Fragments arranged by size: 478 82 29 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 310 591 Size: 310 281 AlwNI CAG_nnn'CTG Cuts at: 0 399 591 Size: 399 192 ApaI G_GGCC'C Cuts at: 0 10 591 Size: 10 581 AvaI C'yCGr_G Cuts at: 0 126 228 591 Size: 126 102 363 Fragments arranged by size: 363 126 102 BanI G'GyrC_C Cuts at: 0 586 591 Size: 586 5 BanII G_rGCy'C Cuts at: 0 10 485 591 Size: 10 475 106 Fragments arranged by size: 475 106 10 BbeI G_GCGC'C Cuts at: 0 590 591 Size: 590 1 BglI GCCn_nnn'nGGC Cuts at: 0 274 591 Size: 274 317 BlpI GC'TnA_GC Cuts at: 0 25 591 Size: 25 566 Bme1580I G_kGCm'C Cuts at: 0 10 591 Size: 10 581 BmgBI CAC'GTC Cuts at: 0 303 591 Size: 303 288 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 570 591 Size: 570 21 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 409 591 Size: 58 351 182 Fragments arranged by size: 351 182 58 BsaAI yAC'GTr Cuts at: 0 384 591 Size: 384 207 BsaHI Gr'CG_yC Cuts at: 0 587 591 Size: 587 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 154 439 591 Size: 154 285 152 Fragments arranged by size: 285 154 152 BseYI C'CCAG_C Cuts at: 0 43 591 Size: 43 548 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 292 364 385 591 Size: 283 9 72 21 206 Fragments arranged by size: 283 206 72 21 9 BsiHKAI G_wGCw'C Cuts at: 0 55 83 591 Size: 55 28 508 Fragments arranged by size: 508 55 28 Bsp1286I G_dGCh'C Cuts at: 0 10 55 83 485 591 Size: 10 45 28 402 106 Fragments arranged by size: 402 106 45 28 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 317 591 Size: 317 274 BsrBI CCG'CTC Cuts at: 0 29 591 Size: 29 562 BsrFI r'CCGG_y Cuts at: 0 199 583 591 Size: 199 384 8 Fragments arranged by size: 384 199 8 BssSI C'ACGA_G Cuts at: 0 117 246 591 Size: 117 129 345 Fragments arranged by size: 345 129 117 BstAPI GCAn_nnn'nTGC Cuts at: 0 270 318 399 591 Size: 270 48 81 192 Fragments arranged by size: 270 192 81 48 BstXI CCAn_nnnn'nTGG Cuts at: 0 18 591 Size: 18 573 BtsI GCAGTG_nn' Cuts at: 0 376 591 Size: 376 215 EarI CTCTTCn'nnn_ Cuts at: 0 43 58 591 Size: 43 15 533 Fragments arranged by size: 533 43 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 82 84 409 562 591 Size: 58 24 2 325 153 29 Fragments arranged by size: 325 153 58 29 24 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 523 591 Size: 523 68 EcoO109I rG'GnC_Cy Cuts at: 0 7 591 Size: 7 584 HaeII r_GCGC'y Cuts at: 0 553 590 591 Size: 553 37 1 Fragments arranged by size: 553 37 1 KasI G'GCGC_C Cuts at: 0 586 591 Size: 586 5 MslI CAynn'nnrTG Cuts at: 0 292 361 591 Size: 292 69 230 Fragments arranged by size: 292 230 69 MspA1I CmG'CkG Cuts at: 0 86 396 591 Size: 86 310 195 Fragments arranged by size: 310 195 86 NaeI GCC'GGC Cuts at: 0 201 591 Size: 201 390 NarI GG'CG_CC Cuts at: 0 587 591 Size: 587 4 NgoMIV G'CCGG_C Cuts at: 0 199 591 Size: 199 392 NspI r_CATG'y Cuts at: 0 220 591 Size: 220 371 PfoI T'CCnGG_A Cuts at: 0 74 591 Size: 74 517 PmlI CAC'GTG Cuts at: 0 384 591 Size: 384 207 PspOMI G'GGCC_C Cuts at: 0 6 591 Size: 6 585 PstI C_TGCA'G Cuts at: 0 314 591 Size: 314 277 SapI GCTCTTCn'nnn_ Cuts at: 0 43 591 Size: 43 548 SbfI CC_TGCA'GG Cuts at: 0 314 591 Size: 314 277 SfcI C'TryA_G Cuts at: 0 310 591 Size: 310 281 SfiI GGCCn_nnn'nGGCC Cuts at: 0 274 591 Size: 274 317 SfoI GGC'GCC Cuts at: 0 588 591 Size: 588 3 SphI G_CATG'C Cuts at: 0 220 591 Size: 220 371 StuI AGG'CCT Cuts at: 0 15 529 591 Size: 15 514 62 Fragments arranged by size: 514 62 15 Enzymes that do cut: AcuI AlfI AlwNI ApaI AvaI BanI BanII BbeI BglI BlpI Bme1580I BmgBI BplI BpmI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BtsI EarI Eco57MI EcoNI EcoO109I HaeII KasI MslI MspA1I NaeI NarI NgoMIV NspI PfoI PmlI PspOMI PstI SapI SbfI SfcI SfiI SfoI SphI StuI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI