Restriction map for
pF1KE2728
(Linear) (Six-base) MAPSORT of: KIEE2728.seq Check: 7670 from: 1 to: 1929
KIEE2728.orf
With 182 enzymes: SgfI *
July 3, 2018 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 204 783 1929
Size: 204 579 1146
Fragments arranged by size:
1146 579 204
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 1222 1793 1929
Size: 357 865 571 136
Fragments arranged by size:
865 571 357 136
AfeI AGC'GCT
Cuts at: 0 129 802 885 1929
Size: 129 673 83 1044
Fragments arranged by size:
1044 673 129 83
AleI CACnn'nnGTG
Cuts at: 0 538 757 1929
Size: 538 219 1172
Fragments arranged by size:
1172 538 219
AlwNI CAG_nnn'CTG
Cuts at: 0 219 285 1096 1929
Size: 219 66 811 833
Fragments arranged by size:
833 811 219 66
ApaI G_GGCC'C
Cuts at: 0 647 1521 1929
Size: 647 874 408
Fragments arranged by size:
874 647 408
AvaI C'yCGr_G
Cuts at: 0 110 711 1018 1677 1929
Size: 110 601 307 659 252
Fragments arranged by size:
659 601 307 252 110
BanI G'GyrC_C
Cuts at: 0 155 1048 1134 1384 1594 1929
Size: 155 893 86 250 210 335
Fragments arranged by size:
893 335 250 210 155 86
BanII G_rGCy'C
Cuts at: 0 647 1169 1246 1521 1632 1929
Size: 647 522 77 275 111 297
Fragments arranged by size:
647 522 297 275 111 77
BbeI G_GCGC'C
Cuts at: 0 159 1388 1929
Size: 159 1229 541
Fragments arranged by size:
1229 541 159
BbsI GAAGACnn'nnnn_
Cuts at: 0 315 1109 1929
Size: 315 794 820
Fragments arranged by size:
820 794 315
BbvCI CC'TCA_GC
Cuts at: 0 131 1054 1929
Size: 131 923 875
Fragments arranged by size:
923 875 131
BglI GCCn_nnn'nGGC
Cuts at: 0 838 1649 1929
Size: 838 811 280
Fragments arranged by size:
838 811 280
BglII A'GATC_T
Cuts at: 0 1002 1929
Size: 1002 927
Bme1580I G_kGCm'C
Cuts at: 0 647 1521 1599 1606 1929
Size: 647 874 78 7 323
Fragments arranged by size:
874 647 323 78 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1031 1494 1879 1929
Size: 382 649 463 385 50
Fragments arranged by size:
649 463 385 382 50
Bpu10I CC'TnA_GC
Cuts at: 0 131 1054 1527 1554 1929
Size: 131 923 473 27 375
Fragments arranged by size:
923 473 375 131 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1929
Size: 238 1691
BsaI GGTCTCn'nnnn_
Cuts at: 0 7 1901 1929
Size: 7 1894 28
Fragments arranged by size:
1894 28 7
BsaAI yAC'GTr
Cuts at: 0 807 1929
Size: 807 1122
BsaHI Gr'CG_yC
Cuts at: 0 156 496 1331 1385 1538 1929
Size: 156 340 835 54 153 391
Fragments arranged by size:
835 391 340 156 153 54
BsaWI w'CCGG_w
Cuts at: 0 511 1929
Size: 511 1418
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1217 1247 1929
Size: 1217 30 682
Fragments arranged by size:
1217 682 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 729 928 1241 1483 1929
Size: 729 199 313 242 446
Fragments arranged by size:
729 446 313 242 199
BseYI C'CCAG_C
Cuts at: 0 219 1281 1402 1522 1929
Size: 219 1062 121 120 407
Fragments arranged by size:
1062 407 219 121 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 507 817 1929
Size: 232 275 310 1112
Fragments arranged by size:
1112 310 275 232
BsiEI CG_ry'CG
Cuts at: 0 209 1929
Size: 209 1720
Bsp1286I G_dGCh'C
Cuts at: 0 647 1169 1246 1521 1599 1606 1632 1929
Size: 647 522 77 275 78 7 26 297
Fragments arranged by size:
647 522 297 275 78 77 26 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 204 783 1929
Size: 204 579 1146
Fragments arranged by size:
1146 579 204
BsrFI r'CCGG_y
Cuts at: 0 152 433 453 1311 1929
Size: 152 281 20 858 618
Fragments arranged by size:
858 618 281 152 20
BsrGI T'GTAC_A
Cuts at: 0 428 1929
Size: 428 1501
BssSI C'ACGA_G
Cuts at: 0 1147 1929
Size: 1147 782
BstBI TT'CG_AA
Cuts at: 0 998 1929
Size: 998 931
BstYI r'GATC_y
Cuts at: 0 1002 1929
Size: 1002 927
BtgI C'CryG_G
Cuts at: 0 8 173 1199 1929
Size: 8 165 1026 730
Fragments arranged by size:
1026 730 165 8
BtsI GCAGTG_nn'
Cuts at: 0 389 1216 1929
Size: 389 827 713
Fragments arranged by size:
827 713 389
DraIII CAC_nnn'GTG
Cuts at: 0 625 1929
Size: 625 1304
DrdI GACnn_nn'nnGTC
Cuts at: 0 52 1759 1929
Size: 52 1707 170
Fragments arranged by size:
1707 170 52
EaeI y'GGCC_r
Cuts at: 0 206 1739 1929
Size: 206 1533 190
Fragments arranged by size:
1533 206 190
EagI C'GGCC_G
Cuts at: 0 206 1929
Size: 206 1723
EarI CTCTTCn'nnn_
Cuts at: 0 316 1929
Size: 316 1613
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 382 1031 1222 1494 1793 1879 1929
Size: 357 25 649 191 272 299 86 50
Fragments arranged by size:
649 357 299 272 191 86 50 25
EcoO109I rG'GnC_Cy
Cuts at: 0 644 1422 1517 1518 1651 1695 1768 1929
Size: 644 778 95 1 133 44 73 161
Fragments arranged by size:
778 644 161 133 95 73 44 1
HaeII r_GCGC'y
Cuts at: 0 131 159 804 887 1363 1388 1929
Size: 131 28 645 83 476 25 541
Fragments arranged by size:
645 541 476 131 83 28 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1217 1249 1277 1309 1619 1651 1929
Size: 1217 32 28 32 310 32 278
Fragments arranged by size:
1217 310 278 32 32 32 28
HincII GTy'rAC
Cuts at: 0 1464 1929
Size: 1464 465
KasI G'GCGC_C
Cuts at: 0 155 1384 1929
Size: 155 1229 545
Fragments arranged by size:
1229 545 155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1869 1929
Size: 212 1657 60
Fragments arranged by size:
1657 212 60
MscI TGG'CCA
Cuts at: 0 1741 1929
Size: 1741 188
MslI CAynn'nnrTG
Cuts at: 0 538 634 757 904 910 1255 1898 1929
Size: 538 96 123 147 6 345 643 31
Fragments arranged by size:
643 538 345 147 123 96 31 6
MspA1I CmG'CkG
Cuts at: 0 1271 1445 1640 1929
Size: 1271 174 195 289
Fragments arranged by size:
1271 289 195 174
NaeI GCC'GGC
Cuts at: 0 154 1313 1929
Size: 154 1159 616
Fragments arranged by size:
1159 616 154
NarI GG'CG_CC
Cuts at: 0 156 1385 1929
Size: 156 1229 544
Fragments arranged by size:
1229 544 156
NgoMIV G'CCGG_C
Cuts at: 0 152 1311 1929
Size: 152 1159 618
Fragments arranged by size:
1159 618 152
NspI r_CATG'y
Cuts at: 0 975 1929
Size: 975 954
PasI CC'CwG_GG
Cuts at: 0 1599 1929
Size: 1599 330
PflMI CCAn_nnn'nTGG
Cuts at: 0 585 1929
Size: 585 1344
PfoI T'CCnGG_A
Cuts at: 0 679 1478 1745 1929
Size: 679 799 267 184
Fragments arranged by size:
799 679 267 184
PpuMI rG'GwC_Cy
Cuts at: 0 1422 1695 1768 1929
Size: 1422 273 73 161
Fragments arranged by size:
1422 273 161 73
PspOMI G'GGCC_C
Cuts at: 0 643 1517 1929
Size: 643 874 412
Fragments arranged by size:
874 643 412
PstI C_TGCA'G
Cuts at: 0 1092 1929
Size: 1092 837
PvuII CAG'CTG
Cuts at: 0 1640 1929
Size: 1640 289
SapI GCTCTTCn'nnn_
Cuts at: 0 316 1929
Size: 316 1613
SexAI A'CCwGG_T
Cuts at: 0 854 1929
Size: 854 1075
SfcI C'TryA_G
Cuts at: 0 1088 1097 1929
Size: 1088 9 832
Fragments arranged by size:
1088 832 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 838 1929
Size: 838 1091
SfoI GGC'GCC
Cuts at: 0 157 1386 1929
Size: 157 1229 543
Fragments arranged by size:
1229 543 157
SmaI CCC'GGG
Cuts at: 0 1020 1679 1929
Size: 1020 659 250
Fragments arranged by size:
1020 659 250
SmlI C'TyrA_G
Cuts at: 0 253 1929
Size: 253 1676
StuI AGG'CCT
Cuts at: 0 238 1929
Size: 238 1691
StyI C'CwwG_G
Cuts at: 0 1160 1379 1727 1929
Size: 1160 219 348 202
Fragments arranged by size:
1160 348 219 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 282 736 1152 1362 1416 1929
Size: 282 454 416 210 54 513
Fragments arranged by size:
513 454 416 282 210 54
TatI w'GTAC_w
Cuts at: 0 407 428 968 1929
Size: 407 21 540 961
Fragments arranged by size:
961 540 407 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1316 1348 1869 1901 1929
Size: 1316 32 521 32 28
Fragments arranged by size:
1316 521 32 32 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1530 1734 1929
Size: 1530 204 195
Fragments arranged by size:
1530 204 195
XmaI C'CCGG_G
Cuts at: 0 1018 1677 1929
Size: 1018 659 252
Fragments arranged by size:
1018 659 252
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BglI BglII Bme1580I BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI Bsp1286I BspMI BsrFI BsrGI BssSI
BstBI BstYI BtgI BtsI DraIII DrdI EaeI EagI
EarI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SapI SexAI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BlpI BmgBI
BmrI BmtI BplI BsaBI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BssHII BstAPI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI