Restriction map for
pF1KE2747
(Linear) (Six-base) MAPSORT of: KIEE2747.seq Check: 1921 from: 1 to: 2598
KIEE2747.orf
With 182 enzymes: SgfI *
April 23, 2019 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 85 2598
Size: 85 2513
AccI GT'mk_AC
Cuts at: 0 1248 2598
Size: 1248 1350
Acc65I G'GTAC_C
Cuts at: 0 1154 2598
Size: 1154 1444
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 2598
Size: 143 2455
AflIII A'CryG_T
Cuts at: 0 858 2598
Size: 858 1740
ApaLI G'TGCA_C
Cuts at: 0 980 2598
Size: 980 1618
ApoI r'AATT_y
Cuts at: 0 719 1264 1939 2598
Size: 719 545 675 659
Fragments arranged by size:
719 675 659 545
AseI AT'TA_AT
Cuts at: 0 433 586 2051 2598
Size: 433 153 1465 547
Fragments arranged by size:
1465 547 433 153
AvaI C'yCGr_G
Cuts at: 0 2189 2410 2598
Size: 2189 221 188
Fragments arranged by size:
2189 221 188
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1138 1171 2598
Size: 1138 33 1427
Fragments arranged by size:
1427 1138 33
BanI G'GyrC_C
Cuts at: 0 1154 2442 2598
Size: 1154 1288 156
Fragments arranged by size:
1288 1154 156
BanII G_rGCy'C
Cuts at: 0 2275 2598
Size: 2275 323
BbeI G_GCGC'C
Cuts at: 0 2446 2598
Size: 2446 152
BbvCI CC'TCA_GC
Cuts at: 0 951 2598
Size: 951 1647
BciVI GTATCCnnnnn_n'
Cuts at: 0 1286 1906 2598
Size: 1286 620 692
Fragments arranged by size:
1286 692 620
BclI T'GATC_A
Cuts at: 0 1364 2598
Size: 1364 1234
BglI GCCn_nnn'nGGC
Cuts at: 0 2451 2495 2598
Size: 2451 44 103
Fragments arranged by size:
2451 103 44
Bme1580I G_kGCm'C
Cuts at: 0 423 984 2598
Size: 423 561 1614
Fragments arranged by size:
1614 561 423
BmgBI CAC'GTC
Cuts at: 0 2430 2598
Size: 2430 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1823 2598
Size: 1823 775
Bpu10I CC'TnA_GC
Cuts at: 0 951 1746 1998 2598
Size: 951 795 252 600
Fragments arranged by size:
951 795 600 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 2598
Size: 675 1923
BsaI GGTCTCn'nnnn_
Cuts at: 0 1968 2123 2598
Size: 1968 155 475
Fragments arranged by size:
1968 475 155
BsaBI GATnn'nnATC
Cuts at: 0 411 2598
Size: 411 2187
BsaHI Gr'CG_yC
Cuts at: 0 2443 2598
Size: 2443 155
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 83 113 2598
Size: 83 30 2485
Fragments arranged by size:
2485 83 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1007 2560 2598
Size: 1007 1553 38
Fragments arranged by size:
1553 1007 38
BseYI C'CCAG_C
Cuts at: 0 1585 2331 2598
Size: 1585 746 267
Fragments arranged by size:
1585 746 267
BsiHKAI G_wGCw'C
Cuts at: 0 984 2598
Size: 984 1614
BsmI GAATG_Cn'
Cuts at: 0 1440 2258 2598
Size: 1440 818 340
Fragments arranged by size:
1440 818 340
Bsp1286I G_dGCh'C
Cuts at: 0 423 984 2275 2598
Size: 423 561 1291 323
Fragments arranged by size:
1291 561 423 323
BspHI T'CATG_A
Cuts at: 0 623 1841 2598
Size: 623 1218 757
Fragments arranged by size:
1218 757 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 85 2598
Size: 85 2513
BsrDI GCAATG_nn'
Cuts at: 0 440 2598
Size: 440 2158
BsrGI T'GTAC_A
Cuts at: 0 638 2598
Size: 638 1960
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1234 2598
Size: 1234 1364
BstBI TT'CG_AA
Cuts at: 0 2253 2598
Size: 2253 345
BstYI r'GATC_y
Cuts at: 0 216 523 2598
Size: 216 307 2075
Fragments arranged by size:
2075 307 216
BtgI C'CryG_G
Cuts at: 0 1150 1724 2598
Size: 1150 574 874
Fragments arranged by size:
1150 874 574
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1590 2598
Size: 1590 1008
BtsI GCAGTG_nn'
Cuts at: 0 149 2350 2598
Size: 149 2201 248
Fragments arranged by size:
2201 248 149
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1248 1283 2598
Size: 1248 35 1315
Fragments arranged by size:
1315 1248 35
DraI TTT'AAA
Cuts at: 0 1527 2598
Size: 1527 1071
EaeI y'GGCC_r
Cuts at: 0 425 1592 2239 2598
Size: 425 1167 647 359
Fragments arranged by size:
1167 647 425 359
EarI CTCTTCn'nnn_
Cuts at: 0 539 1007 1410 2121 2225 2558 2598
Size: 539 468 403 711 104 333 40
Fragments arranged by size:
711 539 468 403 333 104 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 1823 2598
Size: 143 1680 775
Fragments arranged by size:
1680 775 143
EcoNI CCTnn'n_nnAGG
Cuts at: 0 469 685 1012 1872 2011 2598
Size: 469 216 327 860 139 587
Fragments arranged by size:
860 587 469 327 216 139
EcoO109I rG'GnC_Cy
Cuts at: 0 607 847 2598
Size: 607 240 1751
Fragments arranged by size:
1751 607 240
EcoRI G'AATT_C
Cuts at: 0 719 1939 2598
Size: 719 1220 659
Fragments arranged by size:
1220 719 659
EcoRV GAT'ATC
Cuts at: 0 2473 2598
Size: 2473 125
HaeII r_GCGC'y
Cuts at: 0 2446 2598
Size: 2446 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 740 772 925 957 2063 2095
Size: 361 32 347 32 153 32 1106 32
Cuts at: 2095 2105 2137 2598
Size: 10 32 461
Fragments arranged by size:
1106 461 361 347 153 32 32 32
32 32 10
HincII GTy'rAC
Cuts at: 0 1249 2598
Size: 1249 1349
HindIII A'AGCT_T
Cuts at: 0 2248 2598
Size: 2248 350
KasI G'GCGC_C
Cuts at: 0 2442 2598
Size: 2442 156
KpnI G_GTAC'C
Cuts at: 0 1158 2598
Size: 1158 1440
MfeI C'AATT_G
Cuts at: 0 986 2138 2598
Size: 986 1152 460
Fragments arranged by size:
1152 986 460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 1839 2598
Size: 138 1701 759
Fragments arranged by size:
1701 759 138
MscI TGG'CCA
Cuts at: 0 1594 2598
Size: 1594 1004
MslI CAynn'nnrTG
Cuts at: 0 2299 2598
Size: 2299 299
MspA1I CmG'CkG
Cuts at: 0 15 946 955 2598
Size: 15 931 9 1643
Fragments arranged by size:
1643 931 15 9
NarI GG'CG_CC
Cuts at: 0 2443 2598
Size: 2443 155
NcoI C'CATG_G
Cuts at: 0 1150 1724 2598
Size: 1150 574 874
Fragments arranged by size:
1150 874 574
NdeI CA'TA_TG
Cuts at: 0 252 702 2484 2598
Size: 252 450 1782 114
Fragments arranged by size:
1782 450 252 114
NspI r_CATG'y
Cuts at: 0 862 1745 2598
Size: 862 883 853
Fragments arranged by size:
883 862 853
PciI A'CATG_T
Cuts at: 0 858 2598
Size: 858 1740
PflMI CCAn_nnn'nTGG
Cuts at: 0 244 1853 2598
Size: 244 1609 745
Fragments arranged by size:
1609 745 244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 2598
Size: 1 32 2565
Fragments arranged by size:
2565 32 1
PpuMI rG'GwC_Cy
Cuts at: 0 607 847 2598
Size: 607 240 1751
Fragments arranged by size:
1751 607 240
PshAI GACnn'nnGTC
Cuts at: 0 1254 1388 2598
Size: 1254 134 1210
Fragments arranged by size:
1254 1210 134
PsiI TTA'TAA
Cuts at: 0 2459 2598
Size: 2459 139
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 153 185 2598
Size: 153 32 2413
Fragments arranged by size:
2413 153 32
PstI C_TGCA'G
Cuts at: 0 156 960 1839 2598
Size: 156 804 879 759
Fragments arranged by size:
879 804 759 156
PvuII CAG'CTG
Cuts at: 0 15 946 955 2598
Size: 15 931 9 1643
Fragments arranged by size:
1643 931 15 9
SalI G'TCGA_C
Cuts at: 0 1247 2598
Size: 1247 1351
SfcI C'TryA_G
Cuts at: 0 33 152 956 968 1419 1835 2598
Size: 33 119 804 12 451 416 763
Fragments arranged by size:
804 763 451 416 119 33 12
SfoI GGC'GCC
Cuts at: 0 2444 2598
Size: 2444 154
SmlI C'TyrA_G
Cuts at: 0 654 2410 2598
Size: 654 1756 188
Fragments arranged by size:
1756 654 188
SphI G_CATG'C
Cuts at: 0 1745 2598
Size: 1745 853
SspI AAT'ATT
Cuts at: 0 1233 2598
Size: 1233 1365
StyI C'CwwG_G
Cuts at: 0 1150 1724 2438 2449 2598
Size: 1150 574 714 11 149
Fragments arranged by size:
1150 714 574 149 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 267 2598
Size: 267 2331
TatI w'GTAC_w
Cuts at: 0 638 2598
Size: 638 1960
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1604 1636 2598
Size: 1604 32 962
Fragments arranged by size:
1604 962 32
XhoI C'TCGA_G
Cuts at: 0 2410 2598
Size: 2410 188
XmnI GAAnn'nnTTC
Cuts at: 0 233 1169 2598
Size: 233 936 1429
Fragments arranged by size:
1429 936 233
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII ApaLI ApoI AseI
AvaI BaeI BanI BanII BbeI BbvCI BciVI BclI
BglI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrGI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV HaeII Hin4I HincII HindIII KasI KpnI
MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI
NspI PciI PflMI PpiI PpuMI PshAI PsiI PsrI
PstI PvuII SalI SfcI SfoI SmlI SphI SspI
StyI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaI AscI AsiSI AvrII BamHI BbsI
BcgI BfrBI BglII BlpI BmrI BmtI BplI BsaAI
BsaWI BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI
BssHII BssSI BstEII BstXI BstZ17I Bsu36I ClaI DraIII
DrdI EagI EciI EcoICRI FalI FseI FspI FspAI
HpaI MluI NaeI NgoMIV NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PspOMI PspXI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI Tth111I XbaI XcmI XmaI ZraI