Restriction map for
pF1KE2752
(Linear) (Six-base) MAPSORT of: KIEE2752.seq Check: 8985 from: 1 to: 3486
KIEE2752.orf
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AccI GT'mk_AC
Cuts at: 0 1244 3486
Size: 1244 2242
Acc65I G'GTAC_C
Cuts at: 0 546 1495 3486
Size: 546 949 1991
Fragments arranged by size:
1991 949 546
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1717 2359 2564 2818 2845 3106 3486
Size: 163 1554 642 205 254 27 261 380
Fragments arranged by size:
1554 642 380 261 254 205 163 27
AflII C'TTAA_G
Cuts at: 0 43 3486
Size: 43 3443
AflIII A'CryG_T
Cuts at: 0 89 104 3486
Size: 89 15 3382
Fragments arranged by size:
3382 89 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 123 133 3486
Size: 123 10 3353
Fragments arranged by size:
3353 123 10
AlwNI CAG_nnn'CTG
Cuts at: 0 622 1347 2760 3430 3486
Size: 622 725 1413 670 56
Fragments arranged by size:
1413 725 670 622 56
ApoI r'AATT_y
Cuts at: 0 1775 2515 3015 3302 3486
Size: 1775 740 500 287 184
Fragments arranged by size:
1775 740 500 287 184
AvaI C'yCGr_G
Cuts at: 0 2234 3486
Size: 2234 1252
BamHI G'GATC_C
Cuts at: 0 285 1552 2811 3486
Size: 285 1267 1259 675
Fragments arranged by size:
1267 1259 675 285
BanI G'GyrC_C
Cuts at: 0 546 1467 1495 2161 2926 3486
Size: 546 921 28 666 765 560
Fragments arranged by size:
921 765 666 560 546 28
BanII G_rGCy'C
Cuts at: 0 1105 2196 3486
Size: 1105 1091 1290
Fragments arranged by size:
1290 1105 1091
BbvCI CC'TCA_GC
Cuts at: 0 2452 3486
Size: 2452 1034
BciVI GTATCCnnnnn_n'
Cuts at: 0 321 3486
Size: 321 3165
BclI T'GATC_A
Cuts at: 0 1328 2075 3486
Size: 1328 747 1411
Fragments arranged by size:
1411 1328 747
BfrBI ATG'CAT
Cuts at: 0 233 331 3486
Size: 233 98 3155
Fragments arranged by size:
3155 233 98
BglI GCCn_nnn'nGGC
Cuts at: 0 930 2284 3486
Size: 930 1354 1202
Fragments arranged by size:
1354 1202 930
BglII A'GATC_T
Cuts at: 0 3374 3486
Size: 3374 112
Bme1580I G_kGCm'C
Cuts at: 0 2164 2280 3486
Size: 2164 116 1206
Fragments arranged by size:
2164 1206 116
BmgBI CAC'GTC
Cuts at: 0 1886 3486
Size: 1886 1600
BmrI ACTGGGnnnn_n'
Cuts at: 0 870 1222 2748 3486
Size: 870 352 1526 738
Fragments arranged by size:
1526 870 738 352
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2032 2181 3486
Size: 2032 149 1305
Fragments arranged by size:
2032 1305 149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 995 1369 1568 2698 3156 3254 3452
Size: 644 351 374 199 1130 458 98 198
Cuts at: 3452 3486
Size: 34
Fragments arranged by size:
1130 644 458 374 351 199 198 98
34
Bpu10I CC'TnA_GC
Cuts at: 0 989 1797 2452 3486
Size: 989 808 655 1034
Fragments arranged by size:
1034 989 808 655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1669 3486
Size: 1669 1817
BsaI GGTCTCn'nnnn_
Cuts at: 0 946 3486
Size: 946 2540
BsaAI yAC'GTr
Cuts at: 0 90 107 2044 3486
Size: 90 17 1937 1442
Fragments arranged by size:
1937 1442 90 17
BsaHI Gr'CG_yC
Cuts at: 0 53 3486
Size: 53 3433
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1440 1470 2671 2701 3425 3455 3486
Size: 1440 30 1201 30 724 30 31
Fragments arranged by size:
1440 1201 724 31 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1075 2397 2429 2469 2707 3486
Size: 1075 1322 32 40 238 779
Fragments arranged by size:
1322 1075 779 238 40 32
BseYI C'CCAG_C
Cuts at: 0 776 2507 3486
Size: 776 1731 979
Fragments arranged by size:
1731 979 776
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 980 2260 3486
Size: 129 851 1280 1226
Fragments arranged by size:
1280 1226 851 129
BsiEI CG_ry'CG
Cuts at: 0 949 3486
Size: 949 2537
BsiHKAI G_wGCw'C
Cuts at: 0 26 1279 2971 3486
Size: 26 1253 1692 515
Fragments arranged by size:
1692 1253 515 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1129 1660 3486
Size: 1129 531 1826
Fragments arranged by size:
1826 1129 531
Bsp1286I G_dGCh'C
Cuts at: 0 26 1105 1279 2164 2196 2280 2971 3486
Size: 26 1079 174 885 32 84 691 515
Fragments arranged by size:
1079 885 691 515 174 84 32 26
BsrBI CCG'CTC
Cuts at: 0 945 3486
Size: 945 2541
BsrDI GCAATG_nn'
Cuts at: 0 144 3076 3486
Size: 144 2932 410
Fragments arranged by size:
2932 410 144
BsrFI r'CCGG_y
Cuts at: 0 49 3486
Size: 49 3437
BssSI C'ACGA_G
Cuts at: 0 2092 3486
Size: 2092 1394
BstBI TT'CG_AA
Cuts at: 0 2181 3486
Size: 2181 1305
BstEII G'GTnAC_C
Cuts at: 0 771 3486
Size: 771 2715
BstXI CCAn_nnnn'nTGG
Cuts at: 0 410 3486
Size: 410 3076
BstYI r'GATC_y
Cuts at: 0 285 1552 1683 2132 2728 2811 3374 3486
Size: 285 1267 131 449 596 83 563 112
Fragments arranged by size:
1267 596 563 449 285 131 112 83
Bsu36I CC'TnA_GG
Cuts at: 0 926 1194 3182 3486
Size: 926 268 1988 304
Fragments arranged by size:
1988 926 304 268
BtgI C'CryG_G
Cuts at: 0 277 1923 2022 3486
Size: 277 1646 99 1464
Fragments arranged by size:
1646 1464 277 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 74 1624 3486
Size: 74 1550 1862
Fragments arranged by size:
1862 1550 74
BtsI GCAGTG_nn'
Cuts at: 0 1056 1607 3486
Size: 1056 551 1879
Fragments arranged by size:
1879 1056 551
ClaI AT'CG_AT
Cuts at: 0 3116 3486
Size: 3116 370
DraI TTT'AAA
Cuts at: 0 2534 3486
Size: 2534 952
DraIII CAC_nnn'GTG
Cuts at: 0 963 1467 3486
Size: 963 504 2019
Fragments arranged by size:
2019 963 504
DrdI GACnn_nn'nnGTC
Cuts at: 0 3469 3486
Size: 3469 17
EaeI y'GGCC_r
Cuts at: 0 946 2096 3206 3407 3486
Size: 946 1150 1110 201 79
Fragments arranged by size:
1150 1110 946 201 79
EagI C'GGCC_G
Cuts at: 0 946 3486
Size: 946 2540
EarI CTCTTCn'nnn_
Cuts at: 0 584 1267 1310 1396 2546 3486
Size: 584 683 43 86 1150 940
Fragments arranged by size:
1150 940 683 584 86 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2758 3486
Size: 2758 728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 644 995 1369 1568 1717 2359 2564
Size: 163 481 351 374 199 149 642 205
Cuts at: 2564 2698 2818 2845 3106 3156 3254 3452 3486
Size: 134 120 27 261 50 98 198 34
Fragments arranged by size:
642 481 374 351 261 205 199 198
163 149 134 120 98 50 34 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 844 925 1024 1193 3486
Size: 844 81 99 169 2293
Fragments arranged by size:
2293 844 169 99 81
EcoO109I rG'GnC_Cy
Cuts at: 0 835 1701 3178 3486
Size: 835 866 1477 308
Fragments arranged by size:
1477 866 835 308
EcoRI G'AATT_C
Cuts at: 0 1775 3486
Size: 1775 1711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1895 3471 3486
Size: 1895 1576 15
Fragments arranged by size:
1895 1576 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 330 362 520 552 1395 1427 2386 2418
Size: 330 32 158 32 843 32 959 32
Cuts at: 2418 2671 2703 2967 2999 3425 3457 3486
Size: 253 32 264 32 426 32 29
Fragments arranged by size:
959 843 426 330 264 253 158 32
32 32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 0 1633 2394 3486
Size: 1633 761 1092
Fragments arranged by size:
1633 1092 761
HindIII A'AGCT_T
Cuts at: 0 2536 3290 3486
Size: 2536 754 196
Fragments arranged by size:
2536 754 196
KpnI G_GTAC'C
Cuts at: 0 550 1499 3486
Size: 550 949 1987
Fragments arranged by size:
1987 949 550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 3486
Size: 380 3106
MscI TGG'CCA
Cuts at: 0 2098 3409 3486
Size: 2098 1311 77
Fragments arranged by size:
2098 1311 77
MslI CAynn'nnrTG
Cuts at: 0 1595 2254 3486
Size: 1595 659 1232
Fragments arranged by size:
1595 1232 659
MspA1I CmG'CkG
Cuts at: 0 985 2472 3486
Size: 985 1487 1014
Fragments arranged by size:
1487 1014 985
NcoI C'CATG_G
Cuts at: 0 277 1923 2022 3486
Size: 277 1646 99 1464
Fragments arranged by size:
1646 1464 277 99
NdeI CA'TA_TG
Cuts at: 0 2064 3486
Size: 2064 1422
NsiI A_TGCA'T
Cuts at: 0 235 333 3486
Size: 235 98 3153
Fragments arranged by size:
3153 235 98
NspI r_CATG'y
Cuts at: 0 331 2961 3063 3264 3486
Size: 331 2630 102 201 222
Fragments arranged by size:
2630 331 222 201 102
PasI CC'CwG_GG
Cuts at: 0 1020 3486
Size: 1020 2466
PflMI CCAn_nnn'nTGG
Cuts at: 0 1347 1506 3486
Size: 1347 159 1980
Fragments arranged by size:
1980 1347 159
PfoI T'CCnGG_A
Cuts at: 0 972 1340 3486
Size: 972 368 2146
Fragments arranged by size:
2146 972 368
PmlI CAC'GTG
Cuts at: 0 107 2044 3486
Size: 107 1937 1442
Fragments arranged by size:
1937 1442 107
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 392 424 3486
Size: 392 32 3062
Fragments arranged by size:
3062 392 32
PpuMI rG'GwC_Cy
Cuts at: 0 835 1701 3486
Size: 835 866 1785
Fragments arranged by size:
1785 866 835
PshAI GACnn'nnGTC
Cuts at: 0 52 1316 3486
Size: 52 1264 2170
Fragments arranged by size:
2170 1264 52
PvuII CAG'CTG
Cuts at: 0 2472 3486
Size: 2472 1014
SanDI GG'GwC_CC
Cuts at: 0 835 3486
Size: 835 2651
SapI GCTCTTCn'nnn_
Cuts at: 0 1267 1310 3486
Size: 1267 43 2176
Fragments arranged by size:
2176 1267 43
ScaI AGT'ACT
Cuts at: 0 1358 3486
Size: 1358 2128
SexAI A'CCwGG_T
Cuts at: 0 1463 3486
Size: 1463 2023
SfcI C'TryA_G
Cuts at: 0 566 2085 3486
Size: 566 1519 1401
Fragments arranged by size:
1519 1401 566
SmlI C'TyrA_G
Cuts at: 0 43 1648 3486
Size: 43 1605 1838
Fragments arranged by size:
1838 1605 43
SphI G_CATG'C
Cuts at: 0 331 2961 3486
Size: 331 2630 525
Fragments arranged by size:
2630 525 331
StyI C'CwwG_G
Cuts at: 0 277 672 1923 2022 2099 3455 3486
Size: 277 395 1251 99 77 1356 31
Fragments arranged by size:
1356 1251 395 277 99 77 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 925 1012 1618 3314 3486
Size: 925 87 606 1696 172
Fragments arranged by size:
1696 925 606 172 87
TatI w'GTAC_w
Cuts at: 0 851 1356 3486
Size: 851 505 2130
Fragments arranged by size:
2130 851 505
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 117 149 1440 1472 2734 2766 3486
Size: 117 32 1291 32 1262 32 720
Fragments arranged by size:
1291 1262 720 117 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2877 3486
Size: 2877 609
XbaI T'CTAG_A
Cuts at: 0 1515 3486
Size: 1515 1971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1543 3141 3453 3486
Size: 1543 1598 312 33
Fragments arranged by size:
1598 1543 312 33
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AlfI AlwNI ApoI
AvaI BamHI BanI BanII BbvCI BciVI BclI BfrBI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssSI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII
KpnI MmeI MscI MslI MspA1I NcoI NdeI NsiI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PshAI
PvuII SanDI SapI ScaI SexAI SfcI SmlI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BcgI BlpI BmtI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BspMI BsrGI BssHII BstAPI BstZ17I CspCI
EcoICRI EcoRV FseI FspI FspAI HaeII HpaI KasI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
PacI PciI PmeI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI XhoI XmaI XmnI ZraI