(Linear) (Six-base) MAPSORT of: KIEE2752.seq Check: 8985 from: 1 to: 3486 KIEE2752.orf With 182 enzymes: SgfI * August 1, 2019 16:47 .. AccI GT'mk_AC Cuts at: 0 1244 3486 Size: 1244 2242 Acc65I G'GTAC_C Cuts at: 0 546 1495 3486 Size: 546 949 1991 Fragments arranged by size: 1991 949 546 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 1717 2359 2564 2818 2845 3106 3486 Size: 163 1554 642 205 254 27 261 380 Fragments arranged by size: 1554 642 380 261 254 205 163 27 AflII C'TTAA_G Cuts at: 0 43 3486 Size: 43 3443 AflIII A'CryG_T Cuts at: 0 89 104 3486 Size: 89 15 3382 Fragments arranged by size: 3382 89 15 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 123 133 3486 Size: 123 10 3353 Fragments arranged by size: 3353 123 10 AlwNI CAG_nnn'CTG Cuts at: 0 622 1347 2760 3430 3486 Size: 622 725 1413 670 56 Fragments arranged by size: 1413 725 670 622 56 ApoI r'AATT_y Cuts at: 0 1775 2515 3015 3302 3486 Size: 1775 740 500 287 184 Fragments arranged by size: 1775 740 500 287 184 AvaI C'yCGr_G Cuts at: 0 2234 3486 Size: 2234 1252 BamHI G'GATC_C Cuts at: 0 285 1552 2811 3486 Size: 285 1267 1259 675 Fragments arranged by size: 1267 1259 675 285 BanI G'GyrC_C Cuts at: 0 546 1467 1495 2161 2926 3486 Size: 546 921 28 666 765 560 Fragments arranged by size: 921 765 666 560 546 28 BanII G_rGCy'C Cuts at: 0 1105 2196 3486 Size: 1105 1091 1290 Fragments arranged by size: 1290 1105 1091 BbvCI CC'TCA_GC Cuts at: 0 2452 3486 Size: 2452 1034 BciVI GTATCCnnnnn_n' Cuts at: 0 321 3486 Size: 321 3165 BclI T'GATC_A Cuts at: 0 1328 2075 3486 Size: 1328 747 1411 Fragments arranged by size: 1411 1328 747 BfrBI ATG'CAT Cuts at: 0 233 331 3486 Size: 233 98 3155 Fragments arranged by size: 3155 233 98 BglI GCCn_nnn'nGGC Cuts at: 0 930 2284 3486 Size: 930 1354 1202 Fragments arranged by size: 1354 1202 930 BglII A'GATC_T Cuts at: 0 3374 3486 Size: 3374 112 Bme1580I G_kGCm'C Cuts at: 0 2164 2280 3486 Size: 2164 116 1206 Fragments arranged by size: 2164 1206 116 BmgBI CAC'GTC Cuts at: 0 1886 3486 Size: 1886 1600 BmrI ACTGGGnnnn_n' Cuts at: 0 870 1222 2748 3486 Size: 870 352 1526 738 Fragments arranged by size: 1526 870 738 352 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2032 2181 3486 Size: 2032 149 1305 Fragments arranged by size: 2032 1305 149 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 644 995 1369 1568 2698 3156 3254 3452 Size: 644 351 374 199 1130 458 98 198 Cuts at: 3452 3486 Size: 34 Fragments arranged by size: 1130 644 458 374 351 199 198 98 34 Bpu10I CC'TnA_GC Cuts at: 0 989 1797 2452 3486 Size: 989 808 655 1034 Fragments arranged by size: 1034 989 808 655 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1669 3486 Size: 1669 1817 BsaI GGTCTCn'nnnn_ Cuts at: 0 946 3486 Size: 946 2540 BsaAI yAC'GTr Cuts at: 0 90 107 2044 3486 Size: 90 17 1937 1442 Fragments arranged by size: 1937 1442 90 17 BsaHI Gr'CG_yC Cuts at: 0 53 3486 Size: 53 3433 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1440 1470 2671 2701 3425 3455 3486 Size: 1440 30 1201 30 724 30 31 Fragments arranged by size: 1440 1201 724 31 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1075 2397 2429 2469 2707 3486 Size: 1075 1322 32 40 238 779 Fragments arranged by size: 1322 1075 779 238 40 32 BseYI C'CCAG_C Cuts at: 0 776 2507 3486 Size: 776 1731 979 Fragments arranged by size: 1731 979 776 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 129 980 2260 3486 Size: 129 851 1280 1226 Fragments arranged by size: 1280 1226 851 129 BsiEI CG_ry'CG Cuts at: 0 949 3486 Size: 949 2537 BsiHKAI G_wGCw'C Cuts at: 0 26 1279 2971 3486 Size: 26 1253 1692 515 Fragments arranged by size: 1692 1253 515 26 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1129 1660 3486 Size: 1129 531 1826 Fragments arranged by size: 1826 1129 531 Bsp1286I G_dGCh'C Cuts at: 0 26 1105 1279 2164 2196 2280 2971 3486 Size: 26 1079 174 885 32 84 691 515 Fragments arranged by size: 1079 885 691 515 174 84 32 26 BsrBI CCG'CTC Cuts at: 0 945 3486 Size: 945 2541 BsrDI GCAATG_nn' Cuts at: 0 144 3076 3486 Size: 144 2932 410 Fragments arranged by size: 2932 410 144 BsrFI r'CCGG_y Cuts at: 0 49 3486 Size: 49 3437 BssSI C'ACGA_G Cuts at: 0 2092 3486 Size: 2092 1394 BstBI TT'CG_AA Cuts at: 0 2181 3486 Size: 2181 1305 BstEII G'GTnAC_C Cuts at: 0 771 3486 Size: 771 2715 BstXI CCAn_nnnn'nTGG Cuts at: 0 410 3486 Size: 410 3076 BstYI r'GATC_y Cuts at: 0 285 1552 1683 2132 2728 2811 3374 3486 Size: 285 1267 131 449 596 83 563 112 Fragments arranged by size: 1267 596 563 449 285 131 112 83 Bsu36I CC'TnA_GG Cuts at: 0 926 1194 3182 3486 Size: 926 268 1988 304 Fragments arranged by size: 1988 926 304 268 BtgI C'CryG_G Cuts at: 0 277 1923 2022 3486 Size: 277 1646 99 1464 Fragments arranged by size: 1646 1464 277 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 74 1624 3486 Size: 74 1550 1862 Fragments arranged by size: 1862 1550 74 BtsI GCAGTG_nn' Cuts at: 0 1056 1607 3486 Size: 1056 551 1879 Fragments arranged by size: 1879 1056 551 ClaI AT'CG_AT Cuts at: 0 3116 3486 Size: 3116 370 DraI TTT'AAA Cuts at: 0 2534 3486 Size: 2534 952 DraIII CAC_nnn'GTG Cuts at: 0 963 1467 3486 Size: 963 504 2019 Fragments arranged by size: 2019 963 504 DrdI GACnn_nn'nnGTC Cuts at: 0 3469 3486 Size: 3469 17 EaeI y'GGCC_r Cuts at: 0 946 2096 3206 3407 3486 Size: 946 1150 1110 201 79 Fragments arranged by size: 1150 1110 946 201 79 EagI C'GGCC_G Cuts at: 0 946 3486 Size: 946 2540 EarI CTCTTCn'nnn_ Cuts at: 0 584 1267 1310 1396 2546 3486 Size: 584 683 43 86 1150 940 Fragments arranged by size: 1150 940 683 584 86 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2758 3486 Size: 2758 728 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 644 995 1369 1568 1717 2359 2564 Size: 163 481 351 374 199 149 642 205 Cuts at: 2564 2698 2818 2845 3106 3156 3254 3452 3486 Size: 134 120 27 261 50 98 198 34 Fragments arranged by size: 642 481 374 351 261 205 199 198 163 149 134 120 98 50 34 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 844 925 1024 1193 3486 Size: 844 81 99 169 2293 Fragments arranged by size: 2293 844 169 99 81 EcoO109I rG'GnC_Cy Cuts at: 0 835 1701 3178 3486 Size: 835 866 1477 308 Fragments arranged by size: 1477 866 835 308 EcoRI G'AATT_C Cuts at: 0 1775 3486 Size: 1775 1711 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1895 3471 3486 Size: 1895 1576 15 Fragments arranged by size: 1895 1576 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 330 362 520 552 1395 1427 2386 2418 Size: 330 32 158 32 843 32 959 32 Cuts at: 2418 2671 2703 2967 2999 3425 3457 3486 Size: 253 32 264 32 426 32 29 Fragments arranged by size: 959 843 426 330 264 253 158 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 0 1633 2394 3486 Size: 1633 761 1092 Fragments arranged by size: 1633 1092 761 HindIII A'AGCT_T Cuts at: 0 2536 3290 3486 Size: 2536 754 196 Fragments arranged by size: 2536 754 196 KpnI G_GTAC'C Cuts at: 0 550 1499 3486 Size: 550 949 1987 Fragments arranged by size: 1987 949 550 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 380 3486 Size: 380 3106 MscI TGG'CCA Cuts at: 0 2098 3409 3486 Size: 2098 1311 77 Fragments arranged by size: 2098 1311 77 MslI CAynn'nnrTG Cuts at: 0 1595 2254 3486 Size: 1595 659 1232 Fragments arranged by size: 1595 1232 659 MspA1I CmG'CkG Cuts at: 0 985 2472 3486 Size: 985 1487 1014 Fragments arranged by size: 1487 1014 985 NcoI C'CATG_G Cuts at: 0 277 1923 2022 3486 Size: 277 1646 99 1464 Fragments arranged by size: 1646 1464 277 99 NdeI CA'TA_TG Cuts at: 0 2064 3486 Size: 2064 1422 NsiI A_TGCA'T Cuts at: 0 235 333 3486 Size: 235 98 3153 Fragments arranged by size: 3153 235 98 NspI r_CATG'y Cuts at: 0 331 2961 3063 3264 3486 Size: 331 2630 102 201 222 Fragments arranged by size: 2630 331 222 201 102 PasI CC'CwG_GG Cuts at: 0 1020 3486 Size: 1020 2466 PflMI CCAn_nnn'nTGG Cuts at: 0 1347 1506 3486 Size: 1347 159 1980 Fragments arranged by size: 1980 1347 159 PfoI T'CCnGG_A Cuts at: 0 972 1340 3486 Size: 972 368 2146 Fragments arranged by size: 2146 972 368 PmlI CAC'GTG Cuts at: 0 107 2044 3486 Size: 107 1937 1442 Fragments arranged by size: 1937 1442 107 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 392 424 3486 Size: 392 32 3062 Fragments arranged by size: 3062 392 32 PpuMI rG'GwC_Cy Cuts at: 0 835 1701 3486 Size: 835 866 1785 Fragments arranged by size: 1785 866 835 PshAI GACnn'nnGTC Cuts at: 0 52 1316 3486 Size: 52 1264 2170 Fragments arranged by size: 2170 1264 52 PvuII CAG'CTG Cuts at: 0 2472 3486 Size: 2472 1014 SanDI GG'GwC_CC Cuts at: 0 835 3486 Size: 835 2651 SapI GCTCTTCn'nnn_ Cuts at: 0 1267 1310 3486 Size: 1267 43 2176 Fragments arranged by size: 2176 1267 43 ScaI AGT'ACT Cuts at: 0 1358 3486 Size: 1358 2128 SexAI A'CCwGG_T Cuts at: 0 1463 3486 Size: 1463 2023 SfcI C'TryA_G Cuts at: 0 566 2085 3486 Size: 566 1519 1401 Fragments arranged by size: 1519 1401 566 SmlI C'TyrA_G Cuts at: 0 43 1648 3486 Size: 43 1605 1838 Fragments arranged by size: 1838 1605 43 SphI G_CATG'C Cuts at: 0 331 2961 3486 Size: 331 2630 525 Fragments arranged by size: 2630 525 331 StyI C'CwwG_G Cuts at: 0 277 672 1923 2022 2099 3455 3486 Size: 277 395 1251 99 77 1356 31 Fragments arranged by size: 1356 1251 395 277 99 77 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 925 1012 1618 3314 3486 Size: 925 87 606 1696 172 Fragments arranged by size: 1696 925 606 172 87 TatI w'GTAC_w Cuts at: 0 851 1356 3486 Size: 851 505 2130 Fragments arranged by size: 2130 851 505 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 117 149 1440 1472 2734 2766 3486 Size: 117 32 1291 32 1262 32 720 Fragments arranged by size: 1291 1262 720 117 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2877 3486 Size: 2877 609 XbaI T'CTAG_A Cuts at: 0 1515 3486 Size: 1515 1971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1543 3141 3453 3486 Size: 1543 1598 312 33 Fragments arranged by size: 1598 1543 312 33 Enzymes that do cut: AccI Acc65I AcuI AflII AflIII AlfI AlwNI ApoI AvaI BamHI BanI BanII BbvCI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI PshAI PvuII SanDI SapI ScaI SexAI SfcI SmlI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI Enzymes that do not cut: AarI AatII AclI AfeI AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI BlpI BmtI BsaBI BsaWI BsiWI BsmI BspEI BspHI BspMI BsrGI BssHII BstAPI BstZ17I CspCI EcoICRI EcoRV FseI FspI FspAI HaeII HpaI KasI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PciI PmeI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI XhoI XmaI XmnI ZraI