Restriction map for
pF1KE2756
(Linear) (Six-base) MAPSORT of: KIEE2756.seq Check: 3059 from: 1 to: 1467
KIEE2756.orf
With 182 enzymes: SgfI *
July 18, 2019 20:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 110 251 746 914 1467
Size: 6 104 141 495 168 553
Fragments arranged by size:
553 495 168 141 104 6
AfeI AGC'GCT
Cuts at: 0 131 1467
Size: 131 1336
AhdI GACnn_n'nnGTC
Cuts at: 0 314 1467
Size: 314 1153
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1442 1467
Size: 1442 25
ApoI r'AATT_y
Cuts at: 0 261 1467
Size: 261 1206
BanI G'GyrC_C
Cuts at: 0 394 1467
Size: 394 1073
BanII G_rGCy'C
Cuts at: 0 148 572 623 746 1467
Size: 148 424 51 123 721
Fragments arranged by size:
721 424 148 123 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 798 1280 1427 1467
Size: 125 673 482 147 40
Fragments arranged by size:
673 482 147 125 40
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 567 601 1467
Size: 567 34 866
Fragments arranged by size:
866 567 34
BclI T'GATC_A
Cuts at: 0 180 1467
Size: 180 1287
BglI GCCn_nnn'nGGC
Cuts at: 0 349 1467
Size: 349 1118
BlpI GC'TnA_GC
Cuts at: 0 133 740 1467
Size: 133 607 727
Fragments arranged by size:
727 607 133
Bme1580I G_kGCm'C
Cuts at: 0 397 1467
Size: 397 1070
BmgBI CAC'GTC
Cuts at: 0 1088 1467
Size: 1088 379
BmrI ACTGGGnnnn_n'
Cuts at: 0 537 1467
Size: 537 930
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1062 1467
Size: 1062 405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 787 1057 1467
Size: 724 63 270 410
Fragments arranged by size:
724 410 270 63
Bpu10I CC'TnA_GC
Cuts at: 0 429 1467
Size: 429 1038
BsaI GGTCTCn'nnnn_
Cuts at: 0 550 1467
Size: 550 917
BsaBI GATnn'nnATC
Cuts at: 0 179 1467
Size: 179 1288
BsaHI Gr'CG_yC
Cuts at: 0 1258 1467
Size: 1258 209
BsaWI w'CCGG_w
Cuts at: 0 416 1467
Size: 416 1051
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 213 243 1442 1467
Size: 213 30 1199 25
Fragments arranged by size:
1199 213 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 721 841 1264 1467
Size: 181 540 120 423 203
Fragments arranged by size:
540 423 203 181 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 1467
Size: 458 1009
BsiEI CG_ry'CG
Cuts at: 0 353 1467
Size: 353 1114
BsiHKAI G_wGCw'C
Cuts at: 0 148 521 572 623 1367 1467
Size: 148 373 51 51 744 100
Fragments arranged by size:
744 373 148 100 51 51
Bsp1286I G_dGCh'C
Cuts at: 0 148 397 521 572 623 746 1367 1467
Size: 148 249 124 51 51 123 621 100
Fragments arranged by size:
621 249 148 124 123 100 51 51
BspHI T'CATG_A
Cuts at: 0 305 884 1467
Size: 305 579 583
Fragments arranged by size:
583 579 305
BsrBI CCG'CTC
Cuts at: 0 636 1467
Size: 636 831
BsrDI GCAATG_nn'
Cuts at: 0 951 1467
Size: 951 516
BstXI CCAn_nnnn'nTGG
Cuts at: 0 463 1467
Size: 463 1004
BtsI GCAGTG_nn'
Cuts at: 0 345 1068 1467
Size: 345 723 399
Fragments arranged by size:
723 399 345
DraIII CAC_nnn'GTG
Cuts at: 0 531 1204 1467
Size: 531 673 263
Fragments arranged by size:
673 531 263
DrdI GACnn_nn'nnGTC
Cuts at: 0 1282 1467
Size: 1282 185
EaeI y'GGCC_r
Cuts at: 0 8 350 989 1467
Size: 8 342 639 478
Fragments arranged by size:
639 478 342 8
EagI C'GGCC_G
Cuts at: 0 350 1467
Size: 350 1117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 511 1467
Size: 511 956
EcoICRI GAG'CTC
Cuts at: 0 146 570 621 1467
Size: 146 424 51 846
Fragments arranged by size:
846 424 146 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 110 251 724 746 787 914 1057
Size: 6 104 141 473 22 41 127 143
Cuts at: 1057 1467
Size: 410
Fragments arranged by size:
473 410 143 141 127 104 41 22
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 628 1467
Size: 628 839
EcoO109I rG'GnC_Cy
Cuts at: 0 678 872 1467
Size: 678 194 595
Fragments arranged by size:
678 595 194
EcoRV GAT'ATC
Cuts at: 0 1310 1467
Size: 1310 157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 893 1467
Size: 893 574
HaeII r_GCGC'y
Cuts at: 0 133 974 1467
Size: 133 841 493
Fragments arranged by size:
841 493 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 300 332 1467
Size: 300 32 1135
Fragments arranged by size:
1135 300 32
HincII GTy'rAC
Cuts at: 0 1287 1467
Size: 1287 180
HindIII A'AGCT_T
Cuts at: 0 1115 1138 1467
Size: 1115 23 329
Fragments arranged by size:
1115 329 23
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1467
Size: 256 1211
MslI CAynn'nnrTG
Cuts at: 0 718 905 1467
Size: 718 187 562
Fragments arranged by size:
718 562 187
MspA1I CmG'CkG
Cuts at: 0 783 1010 1467
Size: 783 227 457
Fragments arranged by size:
783 457 227
NdeI CA'TA_TG
Cuts at: 0 1003 1467
Size: 1003 464
PfoI T'CCnGG_A
Cuts at: 0 593 1467
Size: 593 874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1442 1467
Size: 1442 25
PpuMI rG'GwC_Cy
Cuts at: 0 678 872 1467
Size: 678 194 595
Fragments arranged by size:
678 595 194
PstI C_TGCA'G
Cuts at: 0 648 1467
Size: 648 819
PvuII CAG'CTG
Cuts at: 0 783 1467
Size: 783 684
SacI G_AGCT'C
Cuts at: 0 148 572 623 1467
Size: 148 424 51 844
Fragments arranged by size:
844 424 148 51
SfcI C'TryA_G
Cuts at: 0 644 1467
Size: 644 823
SspI AAT'ATT
Cuts at: 0 1387 1467
Size: 1387 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 255 519 975 1467
Size: 255 264 456 492
Fragments arranged by size:
492 456 264 255
TatI w'GTAC_w
Cuts at: 0 2 1467
Size: 2 1465
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 517 549 1190 1222 1467
Size: 517 32 641 32 245
Fragments arranged by size:
641 517 245 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 196 1467
Size: 196 1271
Enzymes that do cut:
AcuI AfeI AhdI AloI ApoI BanI BanII BbsI
BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrDI BstXI
BtsI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
MmeI MslI MspA1I NdeI PfoI PpiI PpuMI PstI
PvuII SacI SfcI SspI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BciVI BfrBI
BglII BmtI BpuEI BsaAI BseYI BsiWI BsmI BsmBI
BspEI BspMI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI EarI EcoRI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI