(Linear) (Six-base) MAPSORT of: KIEE2761.seq Check: 1240 from: 1 to: 2505 KIEE2761.orf With 182 enzymes: SgfI * October 3, 2019 17:18 .. AccI GT'mk_AC Cuts at: 0 1107 1344 1731 2505 Size: 1107 237 387 774 Fragments arranged by size: 1107 774 387 237 Acc65I G'GTAC_C Cuts at: 0 544 2505 Size: 544 1961 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1697 2505 Size: 1697 808 AflIII A'CryG_T Cuts at: 0 104 2505 Size: 104 2401 AhdI GACnn_n'nnGTC Cuts at: 0 2425 2505 Size: 2425 80 AlwNI CAG_nnn'CTG Cuts at: 0 815 2505 Size: 815 1690 ApoI r'AATT_y Cuts at: 0 243 1378 2046 2505 Size: 243 1135 668 459 Fragments arranged by size: 1135 668 459 243 AvaI C'yCGr_G Cuts at: 0 668 716 2505 Size: 668 48 1789 Fragments arranged by size: 1789 668 48 BanI G'GyrC_C Cuts at: 0 544 1573 2505 Size: 544 1029 932 Fragments arranged by size: 1029 932 544 BanII G_rGCy'C Cuts at: 0 2059 2505 Size: 2059 446 BbsI GAAGACnn'nnnn_ Cuts at: 0 1618 2503 2505 Size: 1618 885 2 Fragments arranged by size: 1618 885 2 BbvCI CC'TCA_GC Cuts at: 0 2279 2505 Size: 2279 226 BclI T'GATC_A Cuts at: 0 944 2505 Size: 944 1561 BfrBI ATG'CAT Cuts at: 0 24 209 2505 Size: 24 185 2296 Fragments arranged by size: 2296 185 24 BglII A'GATC_T Cuts at: 0 125 2505 Size: 125 2380 BlpI GC'TnA_GC Cuts at: 0 703 1418 2505 Size: 703 715 1087 Fragments arranged by size: 1087 715 703 BmgBI CAC'GTC Cuts at: 0 1111 1729 2505 Size: 1111 618 776 Fragments arranged by size: 1111 776 618 BmrI ACTGGGnnnn_n' Cuts at: 0 1609 2505 Size: 1609 896 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1510 2505 Size: 1510 995 Bpu10I CC'TnA_GC Cuts at: 0 2279 2505 Size: 2279 226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 288 2505 Size: 288 2217 BsaAI yAC'GTr Cuts at: 0 1633 2505 Size: 1633 872 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2459 2489 2505 Size: 2459 30 16 Fragments arranged by size: 2459 30 16 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 824 1248 2505 Size: 824 424 1257 Fragments arranged by size: 1257 824 424 BseYI C'CCAG_C Cuts at: 0 636 1428 2068 2383 2505 Size: 636 792 640 315 122 Fragments arranged by size: 792 640 636 315 122 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2208 2505 Size: 2208 297 BsmI GAATG_Cn' Cuts at: 0 26 1365 2505 Size: 26 1339 1140 Fragments arranged by size: 1339 1140 26 Bsp1286I G_dGCh'C Cuts at: 0 2059 2505 Size: 2059 446 BspHI T'CATG_A Cuts at: 0 623 1461 2505 Size: 623 838 1044 Fragments arranged by size: 1044 838 623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1926 2505 Size: 1926 579 BsrDI GCAATG_nn' Cuts at: 0 2123 2505 Size: 2123 382 BssSI C'ACGA_G Cuts at: 0 473 1607 1855 2505 Size: 473 1134 248 650 Fragments arranged by size: 1134 650 473 248 BstAPI GCAn_nnn'nTGC Cuts at: 0 427 2505 Size: 427 2078 BstBI TT'CG_AA Cuts at: 0 18 2505 Size: 18 2487 BstYI r'GATC_y Cuts at: 0 125 2505 Size: 125 2380 BtgI C'CryG_G Cuts at: 0 1802 2063 2505 Size: 1802 261 442 Fragments arranged by size: 1802 442 261 BtsI GCAGTG_nn' Cuts at: 0 183 1694 2505 Size: 183 1511 811 Fragments arranged by size: 1511 811 183 EarI CTCTTCn'nnn_ Cuts at: 0 155 1154 1938 2505 Size: 155 999 784 567 Fragments arranged by size: 999 784 567 155 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1510 1697 2505 Size: 1510 187 808 Fragments arranged by size: 1510 808 187 EcoO109I rG'GnC_Cy Cuts at: 0 493 2505 Size: 493 2012 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1026 2505 Size: 1026 1479 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 765 797 810 842 1018 1050 1261 1293 Size: 765 32 13 32 176 32 211 32 Cuts at: 1293 1443 1475 2411 2443 2505 Size: 150 32 936 32 62 Fragments arranged by size: 936 765 211 176 150 62 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 0 253 2034 2505 Size: 253 1781 471 Fragments arranged by size: 1781 471 253 HindIII A'AGCT_T Cuts at: 0 368 1504 2505 Size: 368 1136 1001 Fragments arranged by size: 1136 1001 368 KpnI G_GTAC'C Cuts at: 0 548 2505 Size: 548 1957 MfeI C'AATT_G Cuts at: 0 1098 2505 Size: 1098 1407 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 115 464 1421 2505 Size: 115 349 957 1084 Fragments arranged by size: 1084 957 349 115 MslI CAynn'nnrTG Cuts at: 0 88 103 1466 1807 2505 Size: 88 15 1363 341 698 Fragments arranged by size: 1363 698 341 88 15 MspA1I CmG'CkG Cuts at: 0 2222 2505 Size: 2222 283 NcoI C'CATG_G Cuts at: 0 1802 2063 2505 Size: 1802 261 442 Fragments arranged by size: 1802 442 261 NsiI A_TGCA'T Cuts at: 0 26 211 2505 Size: 26 185 2294 Fragments arranged by size: 2294 185 26 NspI r_CATG'y Cuts at: 0 108 597 2321 2505 Size: 108 489 1724 184 Fragments arranged by size: 1724 489 184 108 PasI CC'CwG_GG Cuts at: 0 50 1316 2505 Size: 50 1266 1189 Fragments arranged by size: 1266 1189 50 PciI A'CATG_T Cuts at: 0 104 2505 Size: 104 2401 PmlI CAC'GTG Cuts at: 0 1633 2505 Size: 1633 872 PpuMI rG'GwC_Cy Cuts at: 0 493 2505 Size: 493 2012 PsiI TTA'TAA Cuts at: 0 187 1198 1550 2505 Size: 187 1011 352 955 Fragments arranged by size: 1011 955 352 187 PstI C_TGCA'G Cuts at: 0 1650 2505 Size: 1650 855 PvuII CAG'CTG Cuts at: 0 2222 2505 Size: 2222 283 ScaI AGT'ACT Cuts at: 0 1283 2505 Size: 1283 1222 SfcI C'TryA_G Cuts at: 0 1070 1345 1646 1732 2237 2505 Size: 1070 275 301 86 505 268 Fragments arranged by size: 1070 505 301 275 268 86 SmlI C'TyrA_G Cuts at: 0 303 2505 Size: 303 2202 SphI G_CATG'C Cuts at: 0 2321 2505 Size: 2321 184 SspI AAT'ATT Cuts at: 0 468 2505 Size: 468 2037 StyI C'CwwG_G Cuts at: 0 650 1802 1982 2063 2478 2505 Size: 650 1152 180 81 415 27 Fragments arranged by size: 1152 650 415 180 81 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2245 2505 Size: 2245 260 TatI w'GTAC_w Cuts at: 0 319 844 1004 1281 1903 2505 Size: 319 525 160 277 622 602 Fragments arranged by size: 622 602 525 319 277 160 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1593 1625 2505 Size: 1593 32 880 Fragments arranged by size: 1593 880 32 Enzymes that do cut: AccI Acc65I AcuI AflIII AhdI AlwNI ApoI AvaI BanI BanII BbsI BbvCI BclI BfrBI BglII BlpI BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BstBI BstYI BtgI BtsI EarI Eco57MI EcoO109I FalI Hin4I HincII HindIII KpnI MfeI MmeI MslI MspA1I NcoI NsiI NspI PasI PciI PmlI PpuMI PsiI PstI PvuII ScaI SfcI SmlI SphI SspI StyI TaqII TatI TstI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BglI Bme1580I BmtI BplI BsaI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PflMI PfoI PmeI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI