Restriction map for
pF1KE2762
(Linear) (Six-base) MAPSORT of: KIEE2762.seq Check: 215 from: 1 to: 969
KIEE2762.orf
With 182 enzymes: SgfI *
October 3, 2019 17:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 866 969
Size: 866 103
AccI GT'mk_AC
Cuts at: 0 38 629 969
Size: 38 591 340
Fragments arranged by size:
591 340 38
AflIII A'CryG_T
Cuts at: 0 436 701 969
Size: 436 265 268
Fragments arranged by size:
436 268 265
AleI CACnn'nnGTG
Cuts at: 0 571 969
Size: 571 398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 574 969
Size: 574 395
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 436 468 969
Size: 436 32 501
Fragments arranged by size:
501 436 32
AlwNI CAG_nnn'CTG
Cuts at: 0 165 969
Size: 165 804
ApaI G_GGCC'C
Cuts at: 0 923 969
Size: 923 46
AvaI C'yCGr_G
Cuts at: 0 675 804 841 969
Size: 675 129 37 128
Fragments arranged by size:
675 129 128 37
BanII G_rGCy'C
Cuts at: 0 617 923 969
Size: 617 306 46
Fragments arranged by size:
617 306 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 9 43 969
Size: 9 34 926
Fragments arranged by size:
926 34 9
BglI GCCn_nnn'nGGC
Cuts at: 0 100 592 969
Size: 100 492 377
Fragments arranged by size:
492 377 100
Bme1580I G_kGCm'C
Cuts at: 0 356 434 923 969
Size: 356 78 489 46
Fragments arranged by size:
489 356 78 46
BmgBI CAC'GTC
Cuts at: 0 423 429 969
Size: 423 6 540
Fragments arranged by size:
540 423 6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 744 969
Size: 744 225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 969
Size: 463 506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 601 694 969
Size: 112 489 93 275
Fragments arranged by size:
489 275 112 93
BsaI GGTCTCn'nnnn_
Cuts at: 0 715 969
Size: 715 254
BsaAI yAC'GTr
Cuts at: 0 384 777 969
Size: 384 393 192
Fragments arranged by size:
393 384 192
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 349 379 436 466 969
Size: 349 30 57 30 503
Fragments arranged by size:
503 349 57 30 30
BseYI C'CCAG_C
Cuts at: 0 796 969
Size: 796 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 444 595 649 969
Size: 178 266 151 54 320
Fragments arranged by size:
320 266 178 151 54
BsiEI CG_ry'CG
Cuts at: 0 520 587 969
Size: 520 67 382
Fragments arranged by size:
520 382 67
BsiHKAI G_wGCw'C
Cuts at: 0 563 698 785 890 969
Size: 563 135 87 105 79
Fragments arranged by size:
563 135 105 87 79
Bsp1286I G_dGCh'C
Cuts at: 0 356 434 563 617 698 785 890 923
Size: 356 78 129 54 81 87 105 33
Cuts at: 923 969
Size: 46
Fragments arranged by size:
356 129 105 87 81 78 54 46
33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 178 347 641 824 866 969
Size: 178 169 294 183 42 103
Fragments arranged by size:
294 183 178 169 103 42
BsrBI CCG'CTC
Cuts at: 0 522 969
Size: 522 447
BsrGI T'GTAC_A
Cuts at: 0 944 969
Size: 944 25
BssSI C'ACGA_G
Cuts at: 0 718 969
Size: 718 251
BstXI CCAn_nnnn'nTGG
Cuts at: 0 441 969
Size: 441 528
BtgI C'CryG_G
Cuts at: 0 344 623 969
Size: 344 279 346
Fragments arranged by size:
346 344 279
DraIII CAC_nnn'GTG
Cuts at: 0 687 969
Size: 687 282
EaeI y'GGCC_r
Cuts at: 0 80 92 112 257 323 517 584 593
Size: 80 12 20 145 66 194 67 9
Cuts at: 593 638 689 969
Size: 45 51 280
Fragments arranged by size:
280 194 145 80 67 66 51 45
20 12 9
EagI C'GGCC_G
Cuts at: 0 517 584 969
Size: 517 67 385
Fragments arranged by size:
517 385 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 969
Size: 463 506
EcoNI CCTnn'n_nnAGG
Cuts at: 0 927 969
Size: 927 42
EcoO109I rG'GnC_Cy
Cuts at: 0 102 919 920 969
Size: 102 817 1 49
Fragments arranged by size:
817 102 49 1
HaeII r_GCGC'y
Cuts at: 0 201 969
Size: 201 768
MluI A'CGCG_T
Cuts at: 0 436 969
Size: 436 533
MscI TGG'CCA
Cuts at: 0 82 259 595 640 969
Size: 82 177 336 45 329
Fragments arranged by size:
336 329 177 82 45
MslI CAynn'nnrTG
Cuts at: 0 556 571 969
Size: 556 15 398
Fragments arranged by size:
556 398 15
MspA1I CmG'CkG
Cuts at: 0 63 317 579 822 969
Size: 63 254 262 243 147
Fragments arranged by size:
262 254 243 147 63
NspI r_CATG'y
Cuts at: 0 705 969
Size: 705 264
PasI CC'CwG_GG
Cuts at: 0 915 969
Size: 915 54
PciI A'CATG_T
Cuts at: 0 701 969
Size: 701 268
PflMI CCAn_nnn'nTGG
Cuts at: 0 687 969
Size: 687 282
PmlI CAC'GTG
Cuts at: 0 384 777 969
Size: 384 393 192
Fragments arranged by size:
393 384 192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 969
Size: 436 32 501
Fragments arranged by size:
501 436 32
PspOMI G'GGCC_C
Cuts at: 0 919 969
Size: 919 50
PstI C_TGCA'G
Cuts at: 0 497 969
Size: 497 472
PvuII CAG'CTG
Cuts at: 0 579 969
Size: 579 390
ScaI AGT'ACT
Cuts at: 0 847 969
Size: 847 122
SfcI C'TryA_G
Cuts at: 0 493 969
Size: 493 476
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 100 592 969
Size: 100 492 377
Fragments arranged by size:
492 377 100
SmaI CCC'GGG
Cuts at: 0 806 969
Size: 806 163
SmlI C'TyrA_G
Cuts at: 0 127 616 709 969
Size: 127 489 93 260
Fragments arranged by size:
489 260 127 93
StyI C'CwwG_G
Cuts at: 0 536 969
Size: 536 433
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 337 867 969
Size: 337 530 102
Fragments arranged by size:
530 337 102
TatI w'GTAC_w
Cuts at: 0 845 944 969
Size: 845 99 25
Fragments arranged by size:
845 99 25
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 648 969
Size: 342 306 321
Fragments arranged by size:
342 321 306
XmaI C'CCGG_G
Cuts at: 0 804 969
Size: 804 165
Enzymes that do cut:
AarI AccI AflIII AleI AlfI AloI AlwNI ApaI
AvaI BanII BcgI BglI Bme1580I BmgBI BplI BpmI
BpuEI BsaI BsaAI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrGI BssSI BstXI BtgI DraIII
EaeI EagI Eco57MI EcoNI EcoO109I HaeII MluI MscI
MslI MspA1I NspI PasI PciI PflMI PmlI PpiI
PspOMI PstI PvuII ScaI SfcI SfiI SmaI SmlI
StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI BmrI BmtI Bpu10I BsaBI BsaHI BsaWI BseRI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BssHII
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PfoI
PmeI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmnI ZraI