Restriction map for
pF1KE2783
(Linear) (Six-base) MAPSORT of: KIEE2783.seq Check: 6855 from: 1 to: 780
KIEE2783.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 762 780
Size: 762 18
AccI GT'mk_AC
Cuts at: 0 272 780
Size: 272 508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 780
Size: 127 653
AflIII A'CryG_T
Cuts at: 0 347 780
Size: 347 433
AleI CACnn'nnGTG
Cuts at: 0 266 780
Size: 266 514
AlwNI CAG_nnn'CTG
Cuts at: 0 105 380 780
Size: 105 275 400
Fragments arranged by size:
400 275 105
ApaI G_GGCC'C
Cuts at: 0 90 780
Size: 90 690
AvaI C'yCGr_G
Cuts at: 0 57 83 181 685 780
Size: 57 26 98 504 95
Fragments arranged by size:
504 98 95 57 26
BanII G_rGCy'C
Cuts at: 0 90 780
Size: 90 690
BbvCI CC'TCA_GC
Cuts at: 0 373 718 780
Size: 373 345 62
Fragments arranged by size:
373 345 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 532 563 566 597 780
Size: 532 31 3 31 183
Fragments arranged by size:
532 183 31 31 3
Bme1580I G_kGCm'C
Cuts at: 0 90 434 780
Size: 90 344 346
Fragments arranged by size:
346 344 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 780
Size: 575 205
Bpu10I CC'TnA_GC
Cuts at: 0 373 718 780
Size: 373 345 62
Fragments arranged by size:
373 345 62
BsaAI yAC'GTr
Cuts at: 0 581 780
Size: 581 199
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 217 247 780
Size: 217 30 533
Fragments arranged by size:
533 217 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 780
Size: 160 620
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 366 780
Size: 191 175 414
Fragments arranged by size:
414 191 175
BsiEI CG_ry'CG
Cuts at: 0 33 77 780
Size: 33 44 703
Fragments arranged by size:
703 44 33
Bsp1286I G_dGCh'C
Cuts at: 0 90 434 780
Size: 90 344 346
Fragments arranged by size:
346 344 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 762 780
Size: 762 18
BsrBI CCG'CTC
Cuts at: 0 21 414 780
Size: 21 393 366
Fragments arranged by size:
393 366 21
BsrFI r'CCGG_y
Cuts at: 0 32 780
Size: 32 748
BstEII G'GTnAC_C
Cuts at: 0 774 780
Size: 774 6
BtgI C'CryG_G
Cuts at: 0 77 764 780
Size: 77 687 16
Fragments arranged by size:
687 77 16
BtsI GCAGTG_nn'
Cuts at: 0 518 780
Size: 518 262
DraIII CAC_nnn'GTG
Cuts at: 0 468 780
Size: 468 312
EaeI y'GGCC_r
Cuts at: 0 2 74 459 695 780
Size: 2 72 385 236 85
Fragments arranged by size:
385 236 85 72 2
EagI C'GGCC_G
Cuts at: 0 74 780
Size: 74 706
EarI CTCTTCn'nnn_
Cuts at: 0 215 780
Size: 215 565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 575 780
Size: 127 448 205
Fragments arranged by size:
448 205 127
EcoO109I rG'GnC_Cy
Cuts at: 0 53 87 636 780
Size: 53 34 549 144
Fragments arranged by size:
549 144 53 34
HaeII r_GCGC'y
Cuts at: 0 43 780
Size: 43 737
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 780
Size: 217 32 531
Fragments arranged by size:
531 217 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 780
Size: 319 461
MscI TGG'CCA
Cuts at: 0 4 461 780
Size: 4 457 319
Fragments arranged by size:
457 319 4
MslI CAynn'nnrTG
Cuts at: 0 266 754 780
Size: 266 488 26
Fragments arranged by size:
488 266 26
MspA1I CmG'CkG
Cuts at: 0 266 652 780
Size: 266 386 128
Fragments arranged by size:
386 266 128
NspI r_CATG'y
Cuts at: 0 351 456 780
Size: 351 105 324
Fragments arranged by size:
351 324 105
PciI A'CATG_T
Cuts at: 0 347 780
Size: 347 433
PflMI CCAn_nnn'nTGG
Cuts at: 0 338 780
Size: 338 442
PmlI CAC'GTG
Cuts at: 0 581 780
Size: 581 199
PpuMI rG'GwC_Cy
Cuts at: 0 636 780
Size: 636 144
PspOMI G'GGCC_C
Cuts at: 0 86 780
Size: 86 694
PvuII CAG'CTG
Cuts at: 0 652 780
Size: 652 128
SfcI C'TryA_G
Cuts at: 0 198 495 780
Size: 198 297 285
Fragments arranged by size:
297 285 198
SmaI CCC'GGG
Cuts at: 0 85 183 780
Size: 85 98 597
Fragments arranged by size:
597 98 85
SrfI GCCC'GGGC
Cuts at: 0 85 780
Size: 85 695
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 95 127 780
Size: 95 32 653
Fragments arranged by size:
653 95 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 780
Size: 12 768
XmaI C'CCGG_G
Cuts at: 0 83 181 780
Size: 83 98 599
Fragments arranged by size:
599 98 83
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI AvaI
BanII BbvCI BcgI Bme1580I BpmI Bpu10I BsaAI BsaXI
BseRI BsgI BsiEI Bsp1286I BspMI BsrBI BsrFI BstEII
BtgI BtsI DraIII EaeI EagI EarI Eco57MI EcoO109I
HaeII Hin4I MmeI MscI MslI MspA1I NspI PciI
PflMI PmlI PpuMI PspOMI PvuII SfcI SmaI SrfI
TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI
BsaBI BsaHI BsaWI BseYI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI Tth111I XbaI XhoI XmnI
ZraI