Restriction map for
pF1KE2790
(Linear) (Six-base) MAPSORT of: KIEE2790.seq Check: 3572 from: 1 to: 522
KIEE2790.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 454 522
Size: 454 68
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 415 522
Size: 394 21 107
Fragments arranged by size:
394 107 21
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 312 514 522
Size: 312 202 8
Fragments arranged by size:
312 202 8
AvaI C'yCGr_G
Cuts at: 0 160 478 502 511 522
Size: 160 318 24 9 11
Fragments arranged by size:
318 160 24 11 9
BanI G'GyrC_C
Cuts at: 0 263 522
Size: 263 259
BanII G_rGCy'C
Cuts at: 0 107 522
Size: 107 415
BbeI G_GCGC'C
Cuts at: 0 267 522
Size: 267 255
BbsI GAAGACnn'nnnn_
Cuts at: 0 382 522
Size: 382 140
BmrI ACTGGGnnnn_n'
Cuts at: 0 211 522
Size: 211 311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 522
Size: 211 311
BsaHI Gr'CG_yC
Cuts at: 0 264 522
Size: 264 258
BsaWI w'CCGG_w
Cuts at: 0 455 522
Size: 455 67
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 14 145 262 403 522
Size: 14 131 117 141 119
Fragments arranged by size:
141 131 119 117 14
BseYI C'CCAG_C
Cuts at: 0 109 215 522
Size: 109 106 307
Fragments arranged by size:
307 109 106
Bsp1286I G_dGCh'C
Cuts at: 0 107 522
Size: 107 415
BspEI T'CCGG_A
Cuts at: 0 455 522
Size: 455 67
BspHI T'CATG_A
Cuts at: 0 359 522
Size: 359 163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 454 522
Size: 454 68
BsrBI CCG'CTC
Cuts at: 0 348 405 522
Size: 348 57 117
Fragments arranged by size:
348 117 57
BstEII G'GTnAC_C
Cuts at: 0 63 522
Size: 63 459
BstYI r'GATC_y
Cuts at: 0 47 522
Size: 47 475
BtsI GCAGTG_nn'
Cuts at: 0 496 522
Size: 496 26
DrdI GACnn_nn'nnGTC
Cuts at: 0 480 522
Size: 480 42
EarI CTCTTCn'nnn_
Cuts at: 0 239 522
Size: 239 283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 394 415 522
Size: 211 183 21 107
Fragments arranged by size:
211 183 107 21
EcoO109I rG'GnC_Cy
Cuts at: 0 212 522
Size: 212 310
HaeII r_GCGC'y
Cuts at: 0 267 522
Size: 267 255
KasI G'GCGC_C
Cuts at: 0 263 522
Size: 263 259
MslI CAynn'nnrTG
Cuts at: 0 421 522
Size: 421 101
MspA1I CmG'CkG
Cuts at: 0 336 522
Size: 336 186
NarI GG'CG_CC
Cuts at: 0 264 522
Size: 264 258
PflMI CCAn_nnn'nTGG
Cuts at: 0 88 522
Size: 88 434
PfoI T'CCnGG_A
Cuts at: 0 50 188 522
Size: 50 138 334
Fragments arranged by size:
334 138 50
PspXI vC'TCGA_Gb
Cuts at: 0 502 522
Size: 502 20
PstI C_TGCA'G
Cuts at: 0 335 522
Size: 335 187
RsrII CG'GwC_CG
Cuts at: 0 458 522
Size: 458 64
SfcI C'TryA_G
Cuts at: 0 331 522
Size: 331 191
SfoI GGC'GCC
Cuts at: 0 265 522
Size: 265 257
SmlI C'TyrA_G
Cuts at: 0 502 511 522
Size: 502 9 11
Fragments arranged by size:
502 11 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 475 522
Size: 475 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 450 482 522
Size: 450 32 40
Fragments arranged by size:
450 40 32
Tth111I GACn'n_nGTC
Cuts at: 0 466 522
Size: 466 56
XhoI C'TCGA_G
Cuts at: 0 502 511 522
Size: 502 9 11
Fragments arranged by size:
502 11 9
Enzymes that do cut:
AarI AcuI AlfI AvaI BanI BanII BbeI BbsI
BmrI BpmI BsaHI BsaWI BseRI BseYI Bsp1286I BspEI
BspHI BspMI BsrBI BstEII BstYI BtsI DrdI EarI
Eco57MI EcoO109I HaeII KasI MslI MspA1I NarI PflMI
PfoI PspXI PstI RsrII SfcI SfoI SmlI TaqII
TstI Tth111I XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI XbaI XcmI XmaI XmnI
ZraI