Restriction map for
pF1KE2794
(Linear) (Six-base) MAPSORT of: KIEE2794.seq Check: 8501 from: 1 to: 2091
KIEE2794.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AatII G_ACGT'C
Cuts at: 0 1724 2091
Size: 1724 367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1555 2091
Size: 661 894 536
Fragments arranged by size:
894 661 536
AhdI GACnn_n'nnGTC
Cuts at: 0 1212 2091
Size: 1212 879
AleI CACnn'nnGTG
Cuts at: 0 339 2091
Size: 339 1752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 298 706 1267 2091
Size: 298 408 561 824
Fragments arranged by size:
824 561 408 298
AlwNI CAG_nnn'CTG
Cuts at: 0 208 1035 1146 1470 1509 1695 2091
Size: 208 827 111 324 39 186 396
Fragments arranged by size:
827 396 324 208 186 111 39
ApaI G_GGCC'C
Cuts at: 0 241 1404 2091
Size: 241 1163 687
Fragments arranged by size:
1163 687 241
ApoI r'AATT_y
Cuts at: 0 1540 2091
Size: 1540 551
AvaI C'yCGr_G
Cuts at: 0 75 227 287 716 1090 1130 1171 1239
Size: 75 152 60 429 374 40 41 68
Cuts at: 1239 1627 2091
Size: 388 464
Fragments arranged by size:
464 429 388 374 152 75 68 60
41 40
AvrII C'CTAG_G
Cuts at: 0 786 2091
Size: 786 1305
BanI G'GyrC_C
Cuts at: 0 21 602 1777 2091
Size: 21 581 1175 314
Fragments arranged by size:
1175 581 314 21
BanII G_rGCy'C
Cuts at: 0 75 115 241 852 959 1237 1404 2091
Size: 75 40 126 611 107 278 167 687
Fragments arranged by size:
687 611 278 167 126 107 75 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 1012 2091
Size: 1012 1079
BbvCI CC'TCA_GC
Cuts at: 0 166 1066 2091
Size: 166 900 1025
Fragments arranged by size:
1025 900 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 242 973 1007 2091
Size: 208 34 731 34 1084
Fragments arranged by size:
1084 731 208 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 445 1800 2091
Size: 445 1355 291
Fragments arranged by size:
1355 445 291
BglI GCCn_nnn'nGGC
Cuts at: 0 767 1241 2091
Size: 767 474 850
Fragments arranged by size:
850 767 474
BglII A'GATC_T
Cuts at: 0 221 1407 2091
Size: 221 1186 684
Fragments arranged by size:
1186 684 221
BlpI GC'TnA_GC
Cuts at: 0 1618 2091
Size: 1618 473
Bme1580I G_kGCm'C
Cuts at: 0 26 241 685 1246 1404 2091
Size: 26 215 444 561 158 687
Fragments arranged by size:
687 561 444 215 158 26
BmgBI CAC'GTC
Cuts at: 0 780 1023 2091
Size: 780 243 1068
Fragments arranged by size:
1068 780 243
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 499 561 1284 2091
Size: 499 62 723 807
Fragments arranged by size:
807 723 499 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 666 1021 1531 2059 2085 2091
Size: 666 355 510 528 26 6
Fragments arranged by size:
666 528 510 355 26 6
Bpu10I CC'TnA_GC
Cuts at: 0 28 166 1066 2091
Size: 28 138 900 1025
Fragments arranged by size:
1025 900 138 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1181 2091
Size: 1181 910
BsaI GGTCTCn'nnnn_
Cuts at: 0 515 1597 2035 2091
Size: 515 1082 438 56
Fragments arranged by size:
1082 515 438 56
BsaHI Gr'CG_yC
Cuts at: 0 731 797 917 1433 1721 2091
Size: 731 66 120 516 288 370
Fragments arranged by size:
731 516 370 288 120 66
BsaWI w'CCGG_w
Cuts at: 0 11 253 1970 2091
Size: 11 242 1717 121
Fragments arranged by size:
1717 242 121 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1270 1300 2091
Size: 1270 30 791
Fragments arranged by size:
1270 791 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 83 1584 2091
Size: 83 1501 507
Fragments arranged by size:
1501 507 83
BseYI C'CCAG_C
Cuts at: 0 234 317 470 1586 1876 2091
Size: 234 83 153 1116 290 215
Fragments arranged by size:
1116 290 234 215 153 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1779 2091
Size: 1159 620 312
Fragments arranged by size:
1159 620 312
BsiEI CG_ry'CG
Cuts at: 0 254 378 2091
Size: 254 124 1713
Fragments arranged by size:
1713 254 124
BsiHKAI G_wGCw'C
Cuts at: 0 302 2091
Size: 302 1789
BsmBI CGTCTCn'nnnn_
Cuts at: 0 74 2091
Size: 74 2017
Bsp1286I G_dGCh'C
Cuts at: 0 26 75 115 241 302 685 852 959
Size: 26 49 40 126 61 383 167 107
Cuts at: 959 1237 1246 1404 2091
Size: 278 9 158 687
Fragments arranged by size:
687 383 278 167 158 126 107 61
49 40 26 9
BspEI T'CCGG_A
Cuts at: 0 1970 2091
Size: 1970 121
BsrBI CCG'CTC
Cuts at: 0 1089 1329 2091
Size: 1089 240 762
Fragments arranged by size:
1089 762 240
BsrFI r'CCGG_y
Cuts at: 0 599 746 2091
Size: 599 147 1345
Fragments arranged by size:
1345 599 147
BssSI C'ACGA_G
Cuts at: 0 1390 2091
Size: 1390 701
BstAPI GCAn_nnn'nTGC
Cuts at: 0 640 1146 2091
Size: 640 506 945
Fragments arranged by size:
945 640 506
BstXI CCAn_nnnn'nTGG
Cuts at: 0 341 430 2091
Size: 341 89 1661
Fragments arranged by size:
1661 341 89
BstYI r'GATC_y
Cuts at: 0 221 1407 2091
Size: 221 1186 684
Fragments arranged by size:
1186 684 221
Bsu36I CC'TnA_GG
Cuts at: 0 142 242 2091
Size: 142 100 1849
Fragments arranged by size:
1849 142 100
BtgI C'CryG_G
Cuts at: 0 189 812 1157 1285 1436 1679 1822 2091
Size: 189 623 345 128 151 243 143 269
Fragments arranged by size:
623 345 269 243 189 151 143 128
BtsI GCAGTG_nn'
Cuts at: 0 423 2091
Size: 423 1668
EaeI y'GGCC_r
Cuts at: 0 375 938 1439 2091
Size: 375 563 501 652
Fragments arranged by size:
652 563 501 375
EagI C'GGCC_G
Cuts at: 0 375 2091
Size: 375 1716
EarI CTCTTCn'nnn_
Cuts at: 0 60 530 2091
Size: 60 470 1561
Fragments arranged by size:
1561 470 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 475 586 939 1003 2091
Size: 475 111 353 64 1088
Fragments arranged by size:
1088 475 353 111 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 666 1021 1531 1555 2059 2085 2091
Size: 661 5 355 510 24 504 26 6
Fragments arranged by size:
661 510 504 355 26 24 6 5
EcoO109I rG'GnC_Cy
Cuts at: 0 4 238 307 705 944 1400 1818 2091
Size: 4 234 69 398 239 456 418 273
Fragments arranged by size:
456 418 398 273 239 234 69 4
EcoRV GAT'ATC
Cuts at: 0 1077 2091
Size: 1077 1014
FspI TGC'GCA
Cuts at: 0 1153 1255 2091
Size: 1153 102 836
Fragments arranged by size:
1153 836 102
HaeII r_GCGC'y
Cuts at: 0 1300 1344 2091
Size: 1300 44 747
Fragments arranged by size:
1300 747 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 443 475 1198 1230 2091
Size: 443 32 723 32 861
Fragments arranged by size:
861 723 443 32 32
HindIII A'AGCT_T
Cuts at: 0 1886 2091
Size: 1886 205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 2091
Size: 762 1329
MscI TGG'CCA
Cuts at: 0 1441 2091
Size: 1441 650
MslI CAynn'nnrTG
Cuts at: 0 339 2091
Size: 339 1752
MspA1I CmG'CkG
Cuts at: 0 132 198 576 814 931 1032 1044 1125
Size: 132 66 378 238 117 101 12 81
Cuts at: 1125 1353 1455 1556 1964 2091
Size: 228 102 101 408 127
Fragments arranged by size:
408 378 238 228 132 127 117 102
101 101 81 66 12
NaeI GCC'GGC
Cuts at: 0 601 2091
Size: 601 1490
NcoI C'CATG_G
Cuts at: 0 1822 2091
Size: 1822 269
NgoMIV G'CCGG_C
Cuts at: 0 599 2091
Size: 599 1492
NspI r_CATG'y
Cuts at: 0 643 1313 2091
Size: 643 670 778
Fragments arranged by size:
778 670 643
PflMI CCAn_nnn'nTGG
Cuts at: 0 1509 2091
Size: 1509 582
PfoI T'CCnGG_A
Cuts at: 0 725 2091
Size: 725 1366
PpuMI rG'GwC_Cy
Cuts at: 0 307 1818 2091
Size: 307 1511 273
Fragments arranged by size:
1511 307 273
PshAI GACnn'nnGTC
Cuts at: 0 1466 2091
Size: 1466 625
PspOMI G'GGCC_C
Cuts at: 0 237 1400 2091
Size: 237 1163 691
Fragments arranged by size:
1163 691 237
PspXI vC'TCGA_Gb
Cuts at: 0 1090 1171 1627 2091
Size: 1090 81 456 464
Fragments arranged by size:
1090 464 456 81
PstI C_TGCA'G
Cuts at: 0 587 621 662 695 1454 1745 2091
Size: 587 34 41 33 759 291 346
Fragments arranged by size:
759 587 346 291 41 34 33
PvuII CAG'CTG
Cuts at: 0 198 931 1032 1125 1353 1455 1964 2091
Size: 198 733 101 93 228 102 509 127
Fragments arranged by size:
733 509 228 198 127 102 101 93
SacII CC_GC'GG
Cuts at: 0 815 2091
Size: 815 1276
SapI GCTCTTCn'nnn_
Cuts at: 0 530 2091
Size: 530 1561
SbfI CC_TGCA'GG
Cuts at: 0 662 2091
Size: 662 1429
SfcI C'TryA_G
Cuts at: 0 583 617 658 691 1450 1741 2091
Size: 583 34 41 33 759 291 350
Fragments arranged by size:
759 583 350 291 41 34 33
SmaI CCC'GGG
Cuts at: 0 77 718 1132 2091
Size: 77 641 414 959
Fragments arranged by size:
959 641 414 77
SmlI C'TyrA_G
Cuts at: 0 227 1090 1171 1196 1627 2091
Size: 227 863 81 25 431 464
Fragments arranged by size:
863 464 431 227 81 25
SphI G_CATG'C
Cuts at: 0 643 2091
Size: 643 1448
SrfI GCCC'GGGC
Cuts at: 0 718 2091
Size: 718 1373
StyI C'CwwG_G
Cuts at: 0 429 515 756 786 1773 1822 2091
Size: 429 86 241 30 987 49 269
Fragments arranged by size:
987 429 269 241 86 49 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 347 919 1223 2091
Size: 85 262 572 304 868
Fragments arranged by size:
868 572 304 262 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 1975 2007 2091
Size: 1270 32 673 32 84
Fragments arranged by size:
1270 673 84 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 733 2091
Size: 733 1358
XbaI T'CTAG_A
Cuts at: 0 912 2091
Size: 912 1179
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 763 859 2091
Size: 763 96 1232
Fragments arranged by size:
1232 763 96
XhoI C'TCGA_G
Cuts at: 0 227 1090 1171 1627 2091
Size: 227 863 81 456 464
Fragments arranged by size:
863 464 456 227 81
XmaI C'CCGG_G
Cuts at: 0 75 716 1130 2091
Size: 75 641 414 961
Fragments arranged by size:
961 641 414 75
XmnI GAAnn'nnTTC
Cuts at: 0 1540 2091
Size: 1540 551
ZraI GAC'GTC
Cuts at: 0 1722 2091
Size: 1722 369
Enzymes that do cut:
AatII AcuI AhdI AleI AlfI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BbsI BbvCI BcgI BciVI
BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRV FspI HaeII Hin4I
HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI
PvuII SacII SapI SbfI SfcI SmaI SmlI SphI
SrfI StyI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BclI BfrBI BmrI BmtI BsaAI BsaBI BsiWI BsmI
BspHI BspMI BsrDI BsrGI BssHII BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoRI FalI FseI FspAI HincII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PsiI PsrI
PvuI RsrII SacI SalI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SspI StuI SwaI
TatI