(Linear) (Six-base) MAPSORT of: KIEE2802.seq  Check: 3234  from: 1  to: 354



KIEE2802.orf

 With 182 enzymes: SgfI * 

                        August 16, 2021 14:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     354 
   Size:        100     254
 
AflIII A'CryG_T

Cuts at:      0       5     354 
   Size:          5     349
 
AlwNI CAG_nnn'CTG

Cuts at:      0     129     354 
   Size:        129     225
 
AvaI C'yCGr_G

Cuts at:      0     321     354 
   Size:        321      33
 
BanI G'GyrC_C

Cuts at:      0      23     354 
   Size:         23     331
 
BbeI G_GCGC'C

Cuts at:      0      27     354 
   Size:         27     327
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      54      88     354 
   Size:         54      34     266

  Fragments arranged by size: 

                 266      54      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     238     354 
   Size:        238     116
 
BmgBI CAC'GTC

Cuts at:      0       6     354 
   Size:          6     348
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     178     354 
   Size:        178     176
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     354 
   Size:         88     266
 
BsaHI Gr'CG_yC

Cuts at:      0      24      50     354 
   Size:         24      26     304

  Fragments arranged by size: 

                 304      26      24
 
BseYI C'CCAG_C

Cuts at:      0     213     354 
   Size:        213     141
 
BspHI T'CATG_A

Cuts at:      0      14     354 
   Size:         14     340
 
BsrGI T'GTAC_A

Cuts at:      0     131     354 
   Size:        131     223
 
BstBI TT'CG_AA

Cuts at:      0      74     354 
   Size:         74     280
 
BtsI GCAGTG_nn'

Cuts at:      0     336     354 
   Size:        336      18
 
EaeI y'GGCC_r

Cuts at:      0     202     354 
   Size:        202     152
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     354 
   Size:        100     254
 
EcoO109I rG'GnC_Cy

Cuts at:      0     210     258     354 
   Size:        210      48      96

  Fragments arranged by size: 

                 210      96      48
 
HaeII r_GCGC'y

Cuts at:      0      27     354 
   Size:         27     327
 
KasI G'GCGC_C

Cuts at:      0      23     354 
   Size:         23     331
 
MspA1I CmG'CkG

Cuts at:      0     126     354 
   Size:        126     228
 
NarI GG'CG_CC

Cuts at:      0      24     354 
   Size:         24     330
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     150     354 
   Size:        150     204
 
SfoI GGC'GCC

Cuts at:      0      25     354 
   Size:         25     329
 
SmlI C'TyrA_G

Cuts at:      0     103     354 
   Size:        103     251
 
StyI C'CwwG_G

Cuts at:      0      53     354 
   Size:         53     301
 
TatI w'GTAC_w

Cuts at:      0     131     354 
   Size:        131     223
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     225     354 
   Size:        225     129
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     AvaI     BanI     BbeI     BcgI     BglI
    BmgBI     BmrI    BpuEI    BsaHI    BseYI    BspHI    BsrGI    BstBI
     BtsI     EaeI  Eco57MI EcoO109I    HaeII     KasI   MspA1I     NarI
    PflMI     SfoI     SmlI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbsI    BbvCI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI