(Linear) (Six-base) MAPSORT of: KIEE3012.seq  Check: 7761  from: 1  to: 303



KIEE3012.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     303 
   Size:         77     226
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     107     303 
   Size:        107     196
 
AlwNI CAG_nnn'CTG

Cuts at:      0       9     303 
   Size:          9     294
 
ApaI G_GGCC'C

Cuts at:      0     154     303 
   Size:        154     149
 
BanII G_rGCy'C

Cuts at:      0     154     303 
   Size:        154     149
 
BbvCI CC'TCA_GC

Cuts at:      0     290     303 
   Size:        290      13
 
Bme1580I G_kGCm'C

Cuts at:      0     154     303 
   Size:        154     149
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     160     259     303 
   Size:        145      15      99      44

  Fragments arranged by size: 

                 145      99      44      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     290     303 
   Size:        290      13
 
BseYI C'CCAG_C

Cuts at:      0      80     153     177     303 
   Size:         80      73      24     126

  Fragments arranged by size: 

                 126      80      73      24
 
BsiEI CG_ry'CG

Cuts at:      0     279     303 
   Size:        279      24
 
Bsp1286I G_dGCh'C

Cuts at:      0     154     303 
   Size:        154     149
 
BsrBI CCG'CTC

Cuts at:      0      77     303 
   Size:         77     226
 
BssSI C'ACGA_G

Cuts at:      0     202     303 
   Size:        202     101
 
Bsu36I CC'TnA_GG

Cuts at:      0      99     303 
   Size:         99     204
 
BtgI C'CryG_G

Cuts at:      0     232     303 
   Size:        232      71
 
BtsI GCAGTG_nn'

Cuts at:      0     291     303 
   Size:        291      12
 
DraIII CAC_nnn'GTG

Cuts at:      0     204     303 
   Size:        204      99
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     188     303 
   Size:        188     115
 
EaeI y'GGCC_r

Cuts at:      0      44     276     303 
   Size:         44     232      27

  Fragments arranged by size: 

                 232      44      27
 
EagI C'GGCC_G

Cuts at:      0     276     303 
   Size:        276      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     145     160     259     303 
   Size:         77      68      15      99      44

  Fragments arranged by size: 

                  99      77      68      44      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0     120     150     181     190     303 
   Size:        120      30      31       9     113

  Fragments arranged by size: 

                 120     113      31      30       9
 
HindIII A'AGCT_T

Cuts at:      0      59     303 
   Size:         59     244
 
MspA1I CmG'CkG

Cuts at:      0     177     303 
   Size:        177     126
 
NcoI C'CATG_G

Cuts at:      0     232     303 
   Size:        232      71
 
PasI CC'CwG_GG

Cuts at:      0     186     303 
   Size:        186     117
 
PpuMI rG'GwC_Cy

Cuts at:      0     120     181     190     303 
   Size:        120      61       9     113

  Fragments arranged by size: 

                 120     113      61       9
 
PspOMI G'GGCC_C

Cuts at:      0     150     303 
   Size:        150     153
 
PvuII CAG'CTG

Cuts at:      0     177     303 
   Size:        177     126
 
SanDI GG'GwC_CC

Cuts at:      0     181     190     303 
   Size:        181       9     113

  Fragments arranged by size: 

                 181     113       9
 
StyI C'CwwG_G

Cuts at:      0     232     303 
   Size:        232      71
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     120     303 
   Size:        120     183
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApaI    BanII    BbvCI Bme1580I     BpmI
   Bpu10I    BseYI    BsiEI Bsp1286I    BsrBI    BssSI   Bsu36I     BtgI
     BtsI   DraIII     DrdI     EaeI     EagI  Eco57MI EcoO109I  HindIII
   MspA1I     NcoI     PasI    PpuMI   PspOMI    PvuII    SanDI     StyI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI
     DraI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI