Restriction map for
pF1KE3105
(Linear) (Six-base) MAPSORT of: KIEE3105.seq Check: 7219 from: 1 to: 777
KIEE3105.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AatII G_ACGT'C
Cuts at: 0 403 777
Size: 403 374
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 503 777
Size: 70 433 274
Fragments arranged by size:
433 274 70
AflIII A'CryG_T
Cuts at: 0 308 777
Size: 308 469
AlwNI CAG_nnn'CTG
Cuts at: 0 48 777
Size: 48 729
ApaI G_GGCC'C
Cuts at: 0 379 777
Size: 379 398
ApoI r'AATT_y
Cuts at: 0 208 777
Size: 208 569
AvaI C'yCGr_G
Cuts at: 0 124 777
Size: 124 653
BanI G'GyrC_C
Cuts at: 0 84 671 691 777
Size: 84 587 20 86
Fragments arranged by size:
587 86 84 20
BanII G_rGCy'C
Cuts at: 0 27 107 353 379 656 777
Size: 27 80 246 26 277 121
Fragments arranged by size:
277 246 121 80 27 26
BbeI G_GCGC'C
Cuts at: 0 695 777
Size: 695 82
BbvCI CC'TCA_GC
Cuts at: 0 757 777
Size: 757 20
BclI T'GATC_A
Cuts at: 0 239 777
Size: 239 538
Bme1580I G_kGCm'C
Cuts at: 0 379 777
Size: 379 398
BmgBI CAC'GTC
Cuts at: 0 564 777
Size: 564 213
BmrI ACTGGGnnnn_n'
Cuts at: 0 221 356 777
Size: 221 135 421
Fragments arranged by size:
421 221 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 777
Size: 640 137
Bpu10I CC'TnA_GC
Cuts at: 0 355 757 777
Size: 355 402 20
Fragments arranged by size:
402 355 20
BsaAI yAC'GTr
Cuts at: 0 311 753 777
Size: 311 442 24
Fragments arranged by size:
442 311 24
BsaHI Gr'CG_yC
Cuts at: 0 400 692 777
Size: 400 292 85
Fragments arranged by size:
400 292 85
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 508 774 777
Size: 508 266 3
Fragments arranged by size:
508 266 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 777
Size: 451 326
BsmBI CGTCTCn'nnnn_
Cuts at: 0 391 574 777
Size: 391 183 203
Fragments arranged by size:
391 203 183
Bsp1286I G_dGCh'C
Cuts at: 0 27 107 353 379 656 777
Size: 27 80 246 26 277 121
Fragments arranged by size:
277 246 121 80 27 26
BsrFI r'CCGG_y
Cuts at: 0 668 777
Size: 668 109
BstAPI GCAn_nnn'nTGC
Cuts at: 0 733 777
Size: 733 44
BstBI TT'CG_AA
Cuts at: 0 115 777
Size: 115 662
BtgI C'CryG_G
Cuts at: 0 32 675 777
Size: 32 643 102
Fragments arranged by size:
643 102 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 750 777
Size: 750 27
BtsI GCAGTG_nn'
Cuts at: 0 434 777
Size: 434 343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 503 640 777
Size: 70 433 137 137
Fragments arranged by size:
433 137 137 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 167 777
Size: 167 610
EcoO109I rG'GnC_Cy
Cuts at: 0 375 376 777
Size: 375 1 401
Fragments arranged by size:
401 375 1
HaeII r_GCGC'y
Cuts at: 0 695 777
Size: 695 82
KasI G'GCGC_C
Cuts at: 0 691 777
Size: 691 86
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 383 777
Size: 52 331 394
Fragments arranged by size:
394 331 52
MspA1I CmG'CkG
Cuts at: 0 609 777
Size: 609 168
NarI GG'CG_CC
Cuts at: 0 692 777
Size: 692 85
NspI r_CATG'y
Cuts at: 0 625 777
Size: 625 152
PasI CC'CwG_GG
Cuts at: 0 384 777
Size: 384 393
PmlI CAC'GTG
Cuts at: 0 311 777
Size: 311 466
PspOMI G'GGCC_C
Cuts at: 0 375 777
Size: 375 402
PvuII CAG'CTG
Cuts at: 0 609 777
Size: 609 168
SfoI GGC'GCC
Cuts at: 0 693 777
Size: 693 84
SmlI C'TyrA_G
Cuts at: 0 124 777
Size: 124 653
SphI G_CATG'C
Cuts at: 0 625 777
Size: 625 152
SspI AAT'ATT
Cuts at: 0 120 777
Size: 120 657
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 676 777
Size: 676 101
XhoI C'TCGA_G
Cuts at: 0 124 777
Size: 124 653
XmnI GAAnn'nnTTC
Cuts at: 0 476 777
Size: 476 301
ZraI GAC'GTC
Cuts at: 0 401 777
Size: 401 376
Enzymes that do cut:
AatII AcuI AflIII AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbvCI BclI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaAI BsaHI BseRI BsgI BsmBI Bsp1286I BsrFI
BstAPI BstBI BtgI BtgZI BtsI Eco57MI EcoNI EcoO109I
HaeII KasI MmeI MspA1I NarI NspI PasI PmlI
PspOMI PvuII SfoI SmlI SphI SspI TaqII XhoI
XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BfrBI BglI BglII
BlpI BmtI BplI BpuEI BsaI BsaBI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstEII BstXI BstYI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MscI MslI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI StyI SwaI TatI TstI Tth111I XbaI
XcmI XmaI