Restriction map for
pF1KE3196
(Linear) (Six-base) MAPSORT of: KIEE3196.seq Check: 4743 from: 1 to: 1410
KIEE3196.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 933 1410
Size: 933 477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1410
Size: 199 1211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 364 694 865 1410
Size: 364 330 171 545
Fragments arranged by size:
545 364 330 171
AlwNI CAG_nnn'CTG
Cuts at: 0 592 1410
Size: 592 818
ApaI G_GGCC'C
Cuts at: 0 955 1410
Size: 955 455
ApaLI G'TGCA_C
Cuts at: 0 1318 1410
Size: 1318 92
AvaI C'yCGr_G
Cuts at: 0 57 278 1410
Size: 57 221 1132
Fragments arranged by size:
1132 221 57
BamHI G'GATC_C
Cuts at: 0 261 1410
Size: 261 1149
BanI G'GyrC_C
Cuts at: 0 841 1011 1410
Size: 841 170 399
Fragments arranged by size:
841 399 170
BanII G_rGCy'C
Cuts at: 0 131 530 955 1250 1361 1406 1410
Size: 131 399 425 295 111 45 4
Fragments arranged by size:
425 399 295 131 111 45 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 659 1410
Size: 659 751
BbvCI CC'TCA_GC
Cuts at: 0 386 1410
Size: 386 1024
BfrBI ATG'CAT
Cuts at: 0 1113 1410
Size: 1113 297
BglI GCCn_nnn'nGGC
Cuts at: 0 281 1410
Size: 281 1129
BlpI GC'TnA_GC
Cuts at: 0 757 1410
Size: 757 653
Bme1580I G_kGCm'C
Cuts at: 0 844 955 995 1016 1226 1322 1410
Size: 844 111 40 21 210 96 88
Fragments arranged by size:
844 210 111 96 88 40 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 555 1410
Size: 555 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1000 1410
Size: 515 485 410
Fragments arranged by size:
515 485 410
Bpu10I CC'TnA_GC
Cuts at: 0 386 1410
Size: 386 1024
BsaI GGTCTCn'nnnn_
Cuts at: 0 544 1410
Size: 544 866
BsaAI yAC'GTr
Cuts at: 0 369 1125 1410
Size: 369 756 285
Fragments arranged by size:
756 369 285
BsaHI Gr'CG_yC
Cuts at: 0 86 149 813 1106 1410
Size: 86 63 664 293 304
Fragments arranged by size:
664 304 293 86 63
BsaWI w'CCGG_w
Cuts at: 0 359 1410
Size: 359 1051
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 997 1410
Size: 472 525 413
Fragments arranged by size:
525 472 413
BseYI C'CCAG_C
Cuts at: 0 462 470 582 1366 1379 1410
Size: 462 8 112 784 13 31
Fragments arranged by size:
784 462 112 31 13 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 654 706 1033 1064 1118 1327 1410
Size: 391 263 52 327 31 54 209 83
Fragments arranged by size:
391 327 263 209 83 54 52 31
BsiEI CG_ry'CG
Cuts at: 0 454 882 1410
Size: 454 428 528
Fragments arranged by size:
528 454 428
BsiHKAI G_wGCw'C
Cuts at: 0 96 290 758 1322 1410
Size: 96 194 468 564 88
Fragments arranged by size:
564 468 194 96 88
BsmI GAATG_Cn'
Cuts at: 0 1207 1410
Size: 1207 203
BsmBI CGTCTCn'nnnn_
Cuts at: 0 491 1410
Size: 491 919
Bsp1286I G_dGCh'C
Cuts at: 0 96 131 290 530 758 844 955 995
Size: 96 35 159 240 228 86 111 40
Cuts at: 995 1016 1226 1250 1322 1361 1406 1410
Size: 21 210 24 72 39 45 4
Fragments arranged by size:
240 228 210 159 111 96 86 72
45 40 39 35 24 21 4
BspEI T'CCGG_A
Cuts at: 0 359 1410
Size: 359 1051
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 630 719 933 1410
Size: 630 89 214 477
Fragments arranged by size:
630 477 214 89
BsrBI CCG'CTC
Cuts at: 0 7 423 537 1410
Size: 7 416 114 873
Fragments arranged by size:
873 416 114 7
BsrDI GCAATG_nn'
Cuts at: 0 776 1410
Size: 776 634
BsrFI r'CCGG_y
Cuts at: 0 88 137 689 1410
Size: 88 49 552 721
Fragments arranged by size:
721 552 88 49
BssSI C'ACGA_G
Cuts at: 0 511 1142 1410
Size: 511 631 268
Fragments arranged by size:
631 511 268
BstEII G'GTnAC_C
Cuts at: 0 642 1410
Size: 642 768
BstXI CCAn_nnnn'nTGG
Cuts at: 0 628 1410
Size: 628 782
BstYI r'GATC_y
Cuts at: 0 261 1410
Size: 261 1149
BtgI C'CryG_G
Cuts at: 0 104 197 244 292 876 1085 1172 1226
Size: 104 93 47 48 584 209 87 54
Cuts at: 1226 1410
Size: 184
Fragments arranged by size:
584 209 184 104 93 87 54 48
47
BtsI GCAGTG_nn'
Cuts at: 0 1042 1071 1280 1410
Size: 1042 29 209 130
Fragments arranged by size:
1042 209 130 29
DraIII CAC_nnn'GTG
Cuts at: 0 516 972 1410
Size: 516 456 438
Fragments arranged by size:
516 456 438
EaeI y'GGCC_r
Cuts at: 0 879 1169 1410
Size: 879 290 241
Fragments arranged by size:
879 290 241
EagI C'GGCC_G
Cuts at: 0 879 1410
Size: 879 531
EarI CTCTTCn'nnn_
Cuts at: 0 361 751 900 1168 1394 1410
Size: 361 390 149 268 226 16
Fragments arranged by size:
390 361 268 226 149 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 441 1410
Size: 441 969
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 515 1000 1410
Size: 199 316 485 410
Fragments arranged by size:
485 410 316 199
EcoO109I rG'GnC_Cy
Cuts at: 0 224 951 1410
Size: 224 727 459
Fragments arranged by size:
727 459 224
FspI TGC'GCA
Cuts at: 0 868 1410
Size: 868 542
HaeII r_GCGC'y
Cuts at: 0 209 220 1410
Size: 209 11 1190
Fragments arranged by size:
1190 209 11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1410
Size: 434 976
MscI TGG'CCA
Cuts at: 0 1171 1410
Size: 1171 239
MslI CAynn'nnrTG
Cuts at: 0 409 1410
Size: 409 1001
MspA1I CmG'CkG
Cuts at: 0 32 237 246 268 294 470 878 1094
Size: 32 205 9 22 26 176 408 216
Cuts at: 1094 1102 1346 1370 1410
Size: 8 244 24 40
Fragments arranged by size:
408 244 216 205 176 40 32 26
24 22 9 8
NaeI GCC'GGC
Cuts at: 0 139 691 1410
Size: 139 552 719
Fragments arranged by size:
719 552 139
NcoI C'CATG_G
Cuts at: 0 1085 1172 1410
Size: 1085 87 238
Fragments arranged by size:
1085 238 87
NdeI CA'TA_TG
Cuts at: 0 1190 1410
Size: 1190 220
NgoMIV G'CCGG_C
Cuts at: 0 137 689 1410
Size: 137 552 721
Fragments arranged by size:
721 552 137
NsiI A_TGCA'T
Cuts at: 0 1115 1410
Size: 1115 295
PmlI CAC'GTG
Cuts at: 0 369 1410
Size: 369 1041
PpuMI rG'GwC_Cy
Cuts at: 0 224 1410
Size: 224 1186
PspOMI G'GGCC_C
Cuts at: 0 951 1410
Size: 951 459
PstI C_TGCA'G
Cuts at: 0 102 1410
Size: 102 1308
PvuII CAG'CTG
Cuts at: 0 32 1102 1370 1410
Size: 32 1070 268 40
Fragments arranged by size:
1070 268 40 32
SacII CC_GC'GG
Cuts at: 0 247 295 879 1410
Size: 247 48 584 531
Fragments arranged by size:
584 531 247 48
SanDI GG'GwC_CC
Cuts at: 0 224 1410
Size: 224 1186
SapI GCTCTTCn'nnn_
Cuts at: 0 361 1394 1410
Size: 361 1033 16
Fragments arranged by size:
1033 361 16
SfcI C'TryA_G
Cuts at: 0 98 899 1410
Size: 98 801 511
Fragments arranged by size:
801 511 98
SgrAI Cr'CCGG_yG
Cuts at: 0 88 1410
Size: 88 1322
SmaI CCC'GGG
Cuts at: 0 280 1410
Size: 280 1130
SmlI C'TyrA_G
Cuts at: 0 8 1410
Size: 8 1402
StuI AGG'CCT
Cuts at: 0 346 1410
Size: 346 1064
StyI C'CwwG_G
Cuts at: 0 1085 1172 1250 1410
Size: 1085 87 78 160
Fragments arranged by size:
1085 160 87 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 717 1098 1337 1410
Size: 717 381 239 73
Fragments arranged by size:
717 381 239 73
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 227 1331 1410
Size: 227 1104 79
Fragments arranged by size:
1104 227 79
XmaI C'CCGG_G
Cuts at: 0 278 1410
Size: 278 1132
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbsI BbvCI BfrBI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI
BtgI BtsI DraIII EaeI EagI EarI EciI Eco57MI
EcoO109I FspI HaeII MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NsiI PmlI PpuMI PspOMI PstI
PvuII SacII SanDI SapI SfcI SgrAI SmaI SmlI
StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BglII BmgBI BmtI
BplI BpuEI BsaBI BsaXI BsiWI BspHI BsrGI BssHII
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NheI NotI NruI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI
SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmnI ZraI