Restriction map for
pF1KE3269
(Linear) (Six-base) MAPSORT of: KIEE3269.seq Check: 3320 from: 1 to: 2010
KIEE3269.orf
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1761 2010
Size: 1761 249
AatII G_ACGT'C
Cuts at: 0 71 1016 1672 2010
Size: 71 945 656 338
Fragments arranged by size:
945 656 338 71
AccI GT'mk_AC
Cuts at: 0 1211 1685 2010
Size: 1211 474 325
Fragments arranged by size:
1211 474 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1218 1461 1510 1549 1833 2010
Size: 880 338 243 49 39 284 177
Fragments arranged by size:
880 338 284 243 177 49 39
AflIII A'CryG_T
Cuts at: 0 433 443 1273 1745 2010
Size: 433 10 830 472 265
Fragments arranged by size:
830 472 433 265 10
AgeI A'CCGG_T
Cuts at: 0 1865 2010
Size: 1865 145
AhdI GACnn_n'nnGTC
Cuts at: 0 1092 1758 2010
Size: 1092 666 252
Fragments arranged by size:
1092 666 252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 322 2010
Size: 322 1688
AlwNI CAG_nnn'CTG
Cuts at: 0 1404 1880 1914 1978 2010
Size: 1404 476 34 64 32
Fragments arranged by size:
1404 476 64 34 32
ApaI G_GGCC'C
Cuts at: 0 47 1192 1431 1575 2010
Size: 47 1145 239 144 435
Fragments arranged by size:
1145 435 239 144 47
ApaLI G'TGCA_C
Cuts at: 0 795 1446 2010
Size: 795 651 564
Fragments arranged by size:
795 651 564
AvaI C'yCGr_G
Cuts at: 0 328 620 1955 2010
Size: 328 292 1335 55
Fragments arranged by size:
1335 328 292 55
AvrII C'CTAG_G
Cuts at: 0 1128 2010
Size: 1128 882
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1260 1293 2010
Size: 1260 33 717
Fragments arranged by size:
1260 717 33
BanI G'GyrC_C
Cuts at: 0 626 1723 1798 2010
Size: 626 1097 75 212
Fragments arranged by size:
1097 626 212 75
BanII G_rGCy'C
Cuts at: 0 7 47 551 1004 1192 1431 1547 1575
Size: 7 40 504 453 188 239 116 28
Cuts at: 1575 2010
Size: 435
Fragments arranged by size:
504 453 435 239 188 116 40 28
7
BbsI GAAGACnn'nnnn_
Cuts at: 0 402 1303 1839 2010
Size: 402 901 536 171
Fragments arranged by size:
901 536 402 171
BbvCI CC'TCA_GC
Cuts at: 0 1145 2010
Size: 1145 865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 786 820 2010
Size: 786 34 1190
Fragments arranged by size:
1190 786 34
BclI T'GATC_A
Cuts at: 0 425 2010
Size: 425 1585
BglI GCCn_nnn'nGGC
Cuts at: 0 569 1187 1316 1579 1796 2010
Size: 569 618 129 263 217 214
Fragments arranged by size:
618 569 263 217 214 129
BglII A'GATC_T
Cuts at: 0 1151 1625 2010
Size: 1151 474 385
Fragments arranged by size:
1151 474 385
BlpI GC'TnA_GC
Cuts at: 0 646 1039 2010
Size: 646 393 971
Fragments arranged by size:
971 646 393
Bme1580I G_kGCm'C
Cuts at: 0 21 47 443 631 799 1192 1431 1450
Size: 21 26 396 188 168 393 239 19
Cuts at: 1450 1522 1575 1801 2010
Size: 72 53 226 209
Fragments arranged by size:
396 393 239 226 209 188 168 72
53 26 21 19
BmgBI CAC'GTC
Cuts at: 0 1659 2010
Size: 1659 351
BmrI ACTGGGnnnn_n'
Cuts at: 0 1572 2010
Size: 1572 438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 390 2010
Size: 390 1620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 760 856 1018 1324 1902 2010
Size: 171 589 96 162 306 578 108
Fragments arranged by size:
589 578 306 171 162 108 96
Bpu10I CC'TnA_GC
Cuts at: 0 6 727 1145 2010
Size: 6 721 418 865
Fragments arranged by size:
865 721 418 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 988 2010
Size: 736 252 1022
Fragments arranged by size:
1022 736 252
BsaI GGTCTCn'nnnn_
Cuts at: 0 820 1461 2010
Size: 820 641 549
Fragments arranged by size:
820 641 549
BsaAI yAC'GTr
Cuts at: 0 434 906 1746 1794 2010
Size: 434 472 840 48 216
Fragments arranged by size:
840 472 434 216 48
BsaHI Gr'CG_yC
Cuts at: 0 68 1013 1669 1828 2010
Size: 68 945 656 159 182
Fragments arranged by size:
945 656 182 159 68
BsaWI w'CCGG_w
Cuts at: 0 1865 2010
Size: 1865 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 991 1021 1591 1621 2010
Size: 19 30 942 30 570 30 389
Fragments arranged by size:
942 570 389 30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 171 174 232 346 349 352 355 1233
Size: 171 3 58 114 3 3 3 878
Cuts at: 1233 1449 2010
Size: 216 561
Fragments arranged by size:
878 561 216 171 114 58 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 46 709 954 2010
Size: 46 663 245 1056
Fragments arranged by size:
1056 663 245 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 1359 1887 2010
Size: 268 1091 528 123
Fragments arranged by size:
1091 528 268 123
BsiEI CG_ry'CG
Cuts at: 0 1866 2010
Size: 1866 144
BsiHKAI G_wGCw'C
Cuts at: 0 7 551 767 799 1004 1450 1475 1547
Size: 7 544 216 32 205 446 25 72
Cuts at: 1547 2010
Size: 463
Fragments arranged by size:
544 463 446 216 205 72 32 25
7
Bsp1286I G_dGCh'C
Cuts at: 0 7 21 47 443 551 631 767 799
Size: 7 14 26 396 108 80 136 32
Cuts at: 799 1004 1192 1431 1450 1475 1522 1547 1575
Size: 205 188 239 19 25 47 25 28
Cuts at: 1575 1801 2010
Size: 226 209
Fragments arranged by size:
396 239 226 209 205 188 136 108
80 47 32 28 26 25 25 19
14 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1235 1412 1761 2010
Size: 1235 177 349 249
Fragments arranged by size:
1235 349 249 177
BsrBI CCG'CTC
Cuts at: 0 254 278 2010
Size: 254 24 1732
Fragments arranged by size:
1732 254 24
BsrDI GCAATG_nn'
Cuts at: 0 1691 2010
Size: 1691 319
BsrFI r'CCGG_y
Cuts at: 0 563 1865 2010
Size: 563 1302 145
Fragments arranged by size:
1302 563 145
BsrGI T'GTAC_A
Cuts at: 0 1598 2010
Size: 1598 412
BssSI C'ACGA_G
Cuts at: 0 664 895 2010
Size: 664 231 1115
Fragments arranged by size:
1115 664 231
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1914 2010
Size: 1914 96
BstEII G'GTnAC_C
Cuts at: 0 1222 2010
Size: 1222 788
BstYI r'GATC_y
Cuts at: 0 1151 1625 2010
Size: 1151 474 385
Fragments arranged by size:
1151 474 385
Bsu36I CC'TnA_GG
Cuts at: 0 1873 2010
Size: 1873 137
BtgI C'CryG_G
Cuts at: 0 208 485 506 1217 2010
Size: 208 277 21 711 793
Fragments arranged by size:
793 711 277 208 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 507 2010
Size: 507 1503
BtsI GCAGTG_nn'
Cuts at: 0 165 322 558 1938 2010
Size: 165 157 236 1380 72
Fragments arranged by size:
1380 236 165 157 72
DraIII CAC_nnn'GTG
Cuts at: 0 434 1470 1566 2010
Size: 434 1036 96 444
Fragments arranged by size:
1036 444 434 96
EaeI y'GGCC_r
Cuts at: 0 530 2010
Size: 530 1480
EarI CTCTTCn'nnn_
Cuts at: 0 556 985 1237 1480 1972 2010
Size: 556 429 252 243 492 38
Fragments arranged by size:
556 492 429 252 243 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 2010
Size: 252 1758
EcoICRI GAG'CTC
Cuts at: 0 5 549 1002 1545 2010
Size: 5 544 453 543 465
Fragments arranged by size:
544 543 465 453 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 760 856 880 1018 1218 1324 1461
Size: 171 589 96 24 138 200 106 137
Cuts at: 1461 1510 1549 1833 1902 2010
Size: 49 39 284 69 108
Fragments arranged by size:
589 284 200 171 138 137 108 106
96 69 49 39 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1408 2010
Size: 1408 602
EcoO109I rG'GnC_Cy
Cuts at: 0 43 855 1047 1169 1188 1189 1572 2010
Size: 43 812 192 122 19 1 383 438
Fragments arranged by size:
812 438 383 192 122 43 19 1
HaeII r_GCGC'y
Cuts at: 0 653 2010
Size: 653 1357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 1744 1776 2010
Size: 1078 32 634 32 234
Fragments arranged by size:
1078 634 234 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 2010
Size: 281 1729
MslI CAynn'nnrTG
Cuts at: 0 526 1261 1384 1696 1744 2010
Size: 526 735 123 312 48 266
Fragments arranged by size:
735 526 312 266 123 48
MspA1I CmG'CkG
Cuts at: 0 210 590 674 713 723 1361 1481 1604
Size: 210 380 84 39 10 638 120 123
Cuts at: 1604 2010
Size: 406
Fragments arranged by size:
638 406 380 210 123 120 84 39
10
NaeI GCC'GGC
Cuts at: 0 565 2010
Size: 565 1445
NcoI C'CATG_G
Cuts at: 0 1217 2010
Size: 1217 793
NgoMIV G'CCGG_C
Cuts at: 0 563 2010
Size: 563 1447
NspI r_CATG'y
Cuts at: 0 231 303 1277 2010
Size: 231 72 974 733
Fragments arranged by size:
974 733 231 72
PciI A'CATG_T
Cuts at: 0 1273 2010
Size: 1273 737
PfoI T'CCnGG_A
Cuts at: 0 1016 2010
Size: 1016 994
PmlI CAC'GTG
Cuts at: 0 434 906 1746 1794 2010
Size: 434 472 840 48 216
Fragments arranged by size:
840 472 434 216 48
PpuMI rG'GwC_Cy
Cuts at: 0 1047 1169 2010
Size: 1047 122 841
Fragments arranged by size:
1047 841 122
PshAI GACnn'nnGTC
Cuts at: 0 1843 2010
Size: 1843 167
PspOMI G'GGCC_C
Cuts at: 0 43 1188 1427 1571 2010
Size: 43 1145 239 144 439
Fragments arranged by size:
1145 439 239 144 43
PstI C_TGCA'G
Cuts at: 0 172 406 875 1371 1409 1667 2010
Size: 172 234 469 496 38 258 343
Fragments arranged by size:
496 469 343 258 234 172 38
PvuII CAG'CTG
Cuts at: 0 674 713 2010
Size: 674 39 1297
Fragments arranged by size:
1297 674 39
SacI G_AGCT'C
Cuts at: 0 7 551 1004 1547 2010
Size: 7 544 453 543 463
Fragments arranged by size:
544 543 463 453 7
SacII CC_GC'GG
Cuts at: 0 211 2010
Size: 211 1799
SapI GCTCTTCn'nnn_
Cuts at: 0 556 985 1237 1480 2010
Size: 556 429 252 243 530
Fragments arranged by size:
556 530 429 252 243
SfcI C'TryA_G
Cuts at: 0 168 402 450 681 871 1356 1367 1405
Size: 168 234 48 231 190 485 11 38
Cuts at: 1405 1663 2010
Size: 258 347
Fragments arranged by size:
485 347 258 234 231 190 168 48
38 11
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1187 2010
Size: 1187 823
SmaI CCC'GGG
Cuts at: 0 1957 2010
Size: 1957 53
SmlI C'TyrA_G
Cuts at: 0 751 1003 2010
Size: 751 252 1007
Fragments arranged by size:
1007 751 252
SphI G_CATG'C
Cuts at: 0 231 2010
Size: 231 1779
StuI AGG'CCT
Cuts at: 0 687 1553 2010
Size: 687 866 457
Fragments arranged by size:
866 687 457
StyI C'CwwG_G
Cuts at: 0 1128 1217 1763 2010
Size: 1128 89 546 247
Fragments arranged by size:
1128 546 247 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1210 1554 2010
Size: 1210 344 456
Fragments arranged by size:
1210 456 344
TatI w'GTAC_w
Cuts at: 0 1598 2010
Size: 1598 412
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 277 309 2010
Size: 277 32 1701
Fragments arranged by size:
1701 277 32
Tth111I GACn'n_nGTC
Cuts at: 0 493 2010
Size: 493 1517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 609 1717 2010
Size: 609 1108 293
Fragments arranged by size:
1108 609 293
XmaI C'CCGG_G
Cuts at: 0 1955 2010
Size: 1955 55
ZraI GAC'GTC
Cuts at: 0 69 1014 1670 2010
Size: 69 945 656 340
Fragments arranged by size:
945 656 340 69
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI AvaI AvrII BaeI BanI BanII
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PciI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SapI SfcI SfiI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AleI AloI ApoI AscI
AseI AsiSI BamHI BbeI BciVI BfrBI BmtI BsaBI
BsiWI BsmI BsmBI BspEI BspHI BssHII BstBI BstXI
BstZ17I ClaI CspCI DraI DrdI EagI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NarI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI XmnI