Restriction map for
pF1KE3300
(Linear) (Six-base) MAPSORT of: KIEE3300.seq Check: 6458 from: 1 to: 7665
KIEE3300.orf
With 182 enzymes: SgfI *
May 26, 2016 12:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 355 529 589 703 1054 1204 1405 1771
Size: 355 174 60 114 351 150 201 366
Cuts at: 1771 1858 2083 2110 2143 2308 2341 2917 2998
Size: 87 225 27 33 165 33 576 81
Cuts at: 2998 3025 3145 3484 3598 3736 3769 3970 4120
Size: 27 120 339 114 138 33 201 150
Cuts at: 4120 6707 6833 7140 7665
Size: 2587 126 307 525
Fragments arranged by size:
2587 576 525 366 355 351 339 307
225 201 201 174 165 150 150 138
126 120 114 114 87 81 60 33
33 33 27 27
AatII G_ACGT'C
Cuts at: 0 716 1358 5012 5621 6062 7665
Size: 716 642 3654 609 441 1603
Fragments arranged by size:
3654 1603 716 642 609 441
AccI GT'mk_AC
Cuts at: 0 2603 5027 7665
Size: 2603 2424 2638
Fragments arranged by size:
2638 2603 2424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1909 2331 2907 4482 4602 5384 5410 6163
Size: 1909 422 576 1575 120 782 26 753
Cuts at: 6163 7665
Size: 1502
Fragments arranged by size:
1909 1575 1502 782 753 576 422 120
26
AfeI AGC'GCT
Cuts at: 0 3787 5114 7665
Size: 3787 1327 2551
Fragments arranged by size:
3787 2551 1327
AflIII A'CryG_T
Cuts at: 0 2324 2413 2473 2900 4030 4409 7665
Size: 2324 89 60 427 1130 379 3256
Fragments arranged by size:
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AhdI GACnn_n'nnGTC
Cuts at: 0 3693 7557 7665
Size: 3693 3864 108
Fragments arranged by size:
3864 3693 108
AleI CACnn'nnGTG
Cuts at: 0 725 1654 2992 3185 3730 4720 4807 5092
Size: 725 929 1338 193 545 990 87 285
Cuts at: 5092 7665
Size: 2573
Fragments arranged by size:
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87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1117 1450 2467 5561 5908 7665
Size: 1117 333 1017 3094 347 1757
Fragments arranged by size:
3094 1757 1117 1017 347 333
AlwNI CAG_nnn'CTG
Cuts at: 0 465 1320 1493 1946 2510 3050 3263 3422
Size: 465 855 173 453 564 540 213 159
Cuts at: 3422 3458 4169 4479 5541 5937 7101 7188 7335
Size: 36 711 310 1062 396 1164 87 147
Cuts at: 7335 7443 7665
Size: 108 222
Fragments arranged by size:
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396 310 222 213 173 159 147 108
87 36
ApaI G_GGCC'C
Cuts at: 0 163 287 1219 1339 4129 4141 6879 7342
Size: 163 124 932 120 2790 12 2738 463
Cuts at: 7342 7665
Size: 323
Fragments arranged by size:
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12
ApaLI G'TGCA_C
Cuts at: 0 1453 1659 2328 2904 3132 3178 3646 7357
Size: 1453 206 669 576 228 46 468 3711
Cuts at: 7357 7665
Size: 308
Fragments arranged by size:
3711 1453 669 576 468 308 228 206
46
ApoI r'AATT_y
Cuts at: 0 4255 6882 7665
Size: 4255 2627 783
Fragments arranged by size:
4255 2627 783
AvaI C'yCGr_G
Cuts at: 0 556 1203 1953 2113 4354 4696 6206 6757
Size: 556 647 750 160 2241 342 1510 551
Cuts at: 6757 7665
Size: 908
Fragments arranged by size:
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160
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4032 4065 7665
Size: 4032 33 3600
Fragments arranged by size:
4032 3600 33
BamHI G'GATC_C
Cuts at: 0 2930 7665
Size: 2930 4735
BanI G'GyrC_C
Cuts at: 0 15 343 577 691 808 1039 1255 1618
Size: 15 328 234 114 117 231 216 363
Cuts at: 1618 1846 1957 2071 2296 2467 2986 3100 3586
Size: 228 111 114 225 171 519 114 486
Cuts at: 3586 3724 3838 3902 3958 4024 5407 5749 6734
Size: 138 114 64 56 66 1383 342 985
Cuts at: 6734 6841 6954 6990 7509 7665
Size: 107 113 36 519 156
Fragments arranged by size:
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234 231 228 225 216 171 156 138
117 114 114 114 114 113 111 107
66 64 56 36 15
BanII G_rGCy'C
Cuts at: 0 163 287 1153 1219 1303 1339 2629 2761
Size: 163 124 866 66 84 36 1290 132
Cuts at: 2761 2971 3362 3706 4129 4141 4214 4228 4268
Size: 210 391 344 423 12 73 14 40
Cuts at: 4268 4787 5357 5483 6381 6862 6879 7267 7342
Size: 519 570 126 898 481 17 388 75
Cuts at: 7342 7384 7665
Size: 42 281
Fragments arranged by size:
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388 344 281 210 163 132 126 124
84 75 73 66 42 40 36 17
14 12
BbeI G_GCGC'C
Cuts at: 0 19 1043 3906 4028 5753 7665
Size: 19 1024 2863 122 1725 1912
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 4806 5919 7665
Size: 4806 1113 1746
Fragments arranged by size:
4806 1746 1113
BbvCI CC'TCA_GC
Cuts at: 0 190 7665
Size: 190 7475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2026 2060 3576 3610 3868 3902 7665
Size: 2026 34 1516 34 258 34 3763
Fragments arranged by size:
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BfrBI ATG'CAT
Cuts at: 0 1127 4088 5976 6315 7665
Size: 1127 2961 1888 339 1350
Fragments arranged by size:
2961 1888 1350 1127 339
BglI GCCn_nnn'nGGC
Cuts at: 0 98 392 575 707 740 923 1553 1730
Size: 98 294 183 132 33 183 630 177
Cuts at: 1730 1778 1844 2117 2342 2576 2639 2690 2804
Size: 48 66 273 225 234 63 51 114
Cuts at: 2804 2918 3095 3146 3260 3404 3632 3770 4013
Size: 114 177 51 114 144 228 138 243
Cuts at: 4013 4076 4178 6013 7286 7665
Size: 63 102 1835 1273 379
Fragments arranged by size:
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228 225 183 183 177 177 144 138
132 114 114 114 102 98 66 63
63 51 51 48 33
BlpI GC'TnA_GC
Cuts at: 0 4786 7266 7665
Size: 4786 2480 399
Fragments arranged by size:
4786 2480 399
Bme1580I G_kGCm'C
Cuts at: 0 163 270 287 346 662 694 1219 1339
Size: 163 107 17 59 316 32 525 120
Cuts at: 1339 1457 1549 1663 1849 1960 2074 2332 2413
Size: 118 92 114 186 111 114 258 81
Cuts at: 2413 2502 2818 2908 3136 3182 3256 3290 3589
Size: 89 316 90 228 46 74 34 299
Cuts at: 3589 3637 3650 3961 4009 4129 4141 4249 4631
Size: 48 13 311 48 120 12 108 382
Cuts at: 4631 5412 6394 6689 6737 6879 7342 7361 7665
Size: 781 982 295 48 142 463 19 304
Fragments arranged by size:
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304 299 295 258 228 186 163 142
120 120 118 114 114 111 108 107
92 90 89 81 74 59 48 48
48 46 34 32 19 17 13 12
BmgBI CAC'GTC
Cuts at: 0 1236 3285 5094 6636 7326 7397 7665
Size: 1236 2049 1809 1542 690 71 268
Fragments arranged by size:
2049 1809 1542 1236 690 268 71
BmrI ACTGGGnnnn_n'
Cuts at: 0 1571 3231 6108 7345 7431 7665
Size: 1571 1660 2877 1237 86 234
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 615 1386 2574 3093 3579 7623 7665
Size: 615 771 1188 519 486 4044 42
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1806 5053 5473 5704 5863 6052 6628 6846
Size: 1806 3247 420 231 159 189 576 218
Cuts at: 6846 7593 7665
Size: 747 72
Fragments arranged by size:
3247 1806 747 576 420 231 218 189
159 72
Bpu10I CC'TnA_GC
Cuts at: 0 190 7003 7514 7665
Size: 190 6813 511 151
Fragments arranged by size:
6813 511 190 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5365 6430 6511 7665
Size: 5365 1065 81 1154
Fragments arranged by size:
5365 1154 1065 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 1795 2707 5164 5449 5779 6217 7665
Size: 79 1716 912 2457 285 330 438 1448
Fragments arranged by size:
2457 1716 1448 912 438 330 285 79
BsaAI yAC'GTr
Cuts at: 0 225 657 2325 2414 2474 2903 4031 5214
Size: 225 432 1668 89 60 429 1128 1183
Cuts at: 5214 7665
Size: 2451
Fragments arranged by size:
2451 1668 1183 1128 432 429 225 89
60
BsaBI GATnn'nnATC
Cuts at: 0 5938 7665
Size: 5938 1727
BsaHI Gr'CG_yC
Cuts at: 0 16 713 1040 1355 1391 1748 3830 3903
Size: 16 697 327 315 36 357 2082 73
Cuts at: 3903 4025 4925 5009 5603 5618 5750 5989 6059
Size: 122 900 84 594 15 132 239 70
Cuts at: 6059 7447 7604 7665
Size: 1388 157 61
Fragments arranged by size:
2082 1388 900 697 594 357 327 315
239 157 132 122 84 73 70 61
36 16 15
BsaWI w'CCGG_w
Cuts at: 0 5468 5942 7650 7665
Size: 5468 474 1708 15
Fragments arranged by size:
5468 1708 474 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 361 391 1135 1165 1408 1438 1717 1747
Size: 361 30 744 30 243 30 279 30
Cuts at: 1747 1804 1834 2007 2037 2743 2773 3604 3634
Size: 57 30 173 30 706 30 831 30
Cuts at: 3634 4648 4678 7453 7483 7665
Size: 1014 30 2775 30 182
Fragments arranged by size:
2775 1014 831 744 706 361 279 243
182 173 57 30 30 30 30 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2842 3183 3559 4351 4882 4885 5055 5491
Size: 2842 341 376 792 531 3 170 436
Cuts at: 5491 5722 6196 7461 7665
Size: 231 474 1265 204
Fragments arranged by size:
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231 204 170 3
BseYI C'CCAG_C
Cuts at: 0 74 502 1251 1296 2503 3256 3289 3451
Size: 74 428 749 45 1207 753 33 162
Cuts at: 3451 4418 4910 4932 5124 6384 6481 6771 7105
Size: 967 492 22 192 1260 97 290 334
Cuts at: 7105 7124 7145 7342 7492 7665
Size: 19 21 197 150 173
Fragments arranged by size:
1260 1207 967 753 749 492 428 334
290 197 192 173 162 150 97 74
45 33 22 21 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1176 1483 2550 2748 2862 2943 3462 3741
Size: 1176 307 1067 198 114 81 519 279
Cuts at: 3741 3874 4452 4783 5077 5182 5185 5743 6079
Size: 133 578 331 294 105 3 558 336
Cuts at: 6079 6355 6478 7033 7159 7174 7348 7665
Size: 276 123 555 126 15 174 317
Fragments arranged by size:
1176 1067 578 558 555 519 336 331
317 307 294 279 276 198 174 133
126 123 114 105 81 15 3
BsiEI CG_ry'CG
Cuts at: 0 4712 4863 5225 5723 6786 7665
Size: 4712 151 362 498 1063 879
Fragments arranged by size:
4712 1063 879 498 362 151
BsiHKAI G_wGCw'C
Cuts at: 0 1249 1457 1663 1822 2332 2908 2971 3136
Size: 1249 208 206 159 510 576 63 165
Cuts at: 3136 3182 3448 3622 3650 4787 6140 7009 7267
Size: 46 266 174 28 1137 1353 869 258
Cuts at: 7267 7361 7520 7665
Size: 94 159 145
Fragments arranged by size:
1353 1249 1137 869 576 510 266 258
208 206 174 165 159 159 145 94
63 46 28
BsmI GAATG_Cn'
Cuts at: 0 4150 5101 7575 7646 7665
Size: 4150 951 2474 71 19
Fragments arranged by size:
4150 2474 951 71 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1374 5680 6820 7590 7611 7665
Size: 1374 4306 1140 770 21 54
Fragments arranged by size:
4306 1374 1140 770 54 21
Bsp1286I G_dGCh'C
Cuts at: 0 163 270 287 346 662 694 1153 1219
Size: 163 107 17 59 316 32 459 66
Cuts at: 1219 1249 1303 1339 1457 1549 1663 1822 1849
Size: 30 54 36 118 92 114 159 27
Cuts at: 1849 1960 2074 2332 2413 2502 2629 2761 2818
Size: 111 114 258 81 89 127 132 57
Cuts at: 2818 2908 2971 3136 3182 3256 3290 3362 3448
Size: 90 63 165 46 74 34 72 86
Cuts at: 3448 3589 3622 3637 3650 3706 3961 4009 4129
Size: 141 33 15 13 56 255 48 120
Cuts at: 4129 4141 4214 4228 4249 4268 4631 4787 5357
Size: 12 73 14 21 19 363 156 570
Cuts at: 5357 5412 5483 6140 6381 6394 6689 6737 6862
Size: 55 71 657 241 13 295 48 125
Cuts at: 6862 6879 7009 7267 7342 7361 7384 7520 7665
Size: 17 130 258 75 19 23 136 145
Fragments arranged by size:
657 570 459 363 316 295 258 258
255 241 165 163 159 156 145 141
136 132 130 127 125 120 118 114
114 111 107 92 90 89 86 81
75 74 73 72 71 66 63 59
57 56 55 54 48 48 46 36
34 33 32 30 27 23 21 19
19 17 17 15 14 13 13 12
BspEI T'CCGG_A
Cuts at: 0 5942 7650 7665
Size: 5942 1708 15
Fragments arranged by size:
5942 1708 15
BspHI T'CATG_A
Cuts at: 0 5654 7665
Size: 5654 2011
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 97 355 362 529 589 703 940 1054
Size: 97 258 7 167 60 114 237 114
Cuts at: 1054 1204 1405 1771 1858 2083 2110 2143 2308
Size: 150 201 366 87 225 27 33 165
Cuts at: 2308 2341 2917 2998 3025 3145 3484 3598 3736
Size: 33 576 81 27 120 339 114 138
Cuts at: 3736 3769 3970 4060 4120 5564 6707 6833 7140
Size: 33 201 90 60 1444 1143 126 307
Cuts at: 7140 7196 7665
Size: 56 469
Fragments arranged by size:
1444 1143 576 469 366 339 307 258
237 225 201 201 167 165 150 138
126 120 114 114 114 97 90 87
81 60 60 56 33 33 33 27
27 7
BsrBI CCG'CTC
Cuts at: 0 9 648 5652 6414 6947 7665
Size: 9 639 5004 762 533 718
Fragments arranged by size:
5004 762 718 639 533 9
BsrFI r'CCGG_y
Cuts at: 0 524 748 2633 2689 2732 3268 3517 4534
Size: 524 224 1885 56 43 536 249 1017
Cuts at: 4534 4711 5045 5190 5279 5519 5718 6785 7665
Size: 177 334 145 89 240 199 1067 880
Fragments arranged by size:
1885 1067 1017 880 536 524 334 249
240 224 199 177 145 89 56 43
BssSI C'ACGA_G
Cuts at: 0 56 721 7665
Size: 56 665 6944
Fragments arranged by size:
6944 665 56
BstAPI GCAn_nnn'nTGC
Cuts at: 0 200 253 1493 1607 1946 2057 2171 2885
Size: 200 53 1240 114 339 111 114 714
Cuts at: 2885 3050 3944 4169 4187 4479 5055 6563 7188
Size: 165 894 225 18 292 576 1508 625
Cuts at: 7188 7221 7665
Size: 33 444
Fragments arranged by size:
1508 1240 894 714 625 576 444 339
292 225 200 165 114 114 111 53
33 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1401 1629 5094 5961 6703 7350 7665
Size: 1401 228 3465 867 742 647 315
Fragments arranged by size:
3465 1401 867 742 647 315 228
BstYI r'GATC_y
Cuts at: 0 2930 5933 7665
Size: 2930 3003 1732
Fragments arranged by size:
3003 2930 1732
Bsu36I CC'TnA_GG
Cuts at: 0 5309 7665
Size: 5309 2356
BtgI C'CryG_G
Cuts at: 0 510 570 704 945 1035 1086 1550 1839
Size: 510 60 134 241 90 51 464 289
Cuts at: 1839 2178 2573 2661 3525 3628 3834 3968 3987
Size: 339 395 88 864 103 206 134 19
Cuts at: 3987 5003 5013 5174 5372 5483 5570 5628 6017
Size: 1016 10 161 198 111 87 58 389
Cuts at: 6017 6596 6618 7665
Size: 579 22 1047
Fragments arranged by size:
1047 1016 864 579 510 464 395 389
339 289 241 206 198 161 134 134
111 103 90 88 87 60 58 51
22 19 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 81 180 6282 7665
Size: 81 99 6102 1383
Fragments arranged by size:
6102 1383 99 81
BtsI GCAGTG_nn'
Cuts at: 0 492 1455 1683 1794 2679 3387 3873 4402
Size: 492 963 228 111 885 708 486 529
Cuts at: 4402 4489 4629 5056 5077 5530 6649 7665
Size: 87 140 427 21 453 1119 1016
Fragments arranged by size:
1119 1016 963 885 708 529 492 486
453 427 228 140 111 87 21
ClaI AT'CG_AT
Cuts at: 0 1700 1976 2036 7665
Size: 1700 276 60 5629
Fragments arranged by size:
5629 1700 276 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 525 560 6907 6942 7665
Size: 525 35 6347 35 723
Fragments arranged by size:
6347 723 525 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 228 3531 5923 7137 7665
Size: 228 3303 2392 1214 528
Fragments arranged by size:
3303 2392 1214 528 228
DrdI GACnn_nn'nnGTC
Cuts at: 0 5623 7665
Size: 5623 2042
EaeI y'GGCC_r
Cuts at: 0 116 456 546 750 1089 1506 3515 4157
Size: 116 340 90 204 339 417 2009 642
Cuts at: 4157 4506 4709 4833 4860 5096 5222 5292 5720
Size: 349 203 124 27 236 126 70 428
Cuts at: 5720 5801 6014 6200 6749 6783 7118 7278 7665
Size: 81 213 186 549 34 335 160 387
Fragments arranged by size:
2009 642 549 428 417 387 349 340
339 335 236 213 204 203 186 160
126 124 116 90 81 70 34 27
EagI C'GGCC_G
Cuts at: 0 4709 4860 5222 5720 6783 7665
Size: 4709 151 362 498 1063 882
Fragments arranged by size:
4709 1063 882 498 362 151
EarI CTCTTCn'nnn_
Cuts at: 0 4549 5699 7665
Size: 4549 1150 1966
Fragments arranged by size:
4549 1966 1150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 5001 6078 7665
Size: 5001 1077 1587
Fragments arranged by size:
5001 1587 1077
EcoICRI GAG'CTC
Cuts at: 0 2969 4785 7265 7665
Size: 2969 1816 2480 400
Fragments arranged by size:
2969 2480 1816 400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1806 1909 2331 2907 4482 4602 5053 5384
Size: 1806 103 422 576 1575 120 451 331
Cuts at: 5384 5410 5473 5704 5863 6052 6163 6628 6846
Size: 26 63 231 159 189 111 465 218
Cuts at: 6846 7593 7665
Size: 747 72
Fragments arranged by size:
1806 1575 747 576 465 451 422 331
231 218 189 159 120 111 103 72
63 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2143 5362 5842 7665
Size: 2143 3219 480 1823
Fragments arranged by size:
3219 2143 1823 480
EcoO109I rG'GnC_Cy
Cuts at: 0 149 174 284 929 1336 1903 2183 3429
Size: 149 25 110 645 407 567 280 1246
Cuts at: 3429 4126 4138 4202 5448 6036 6522 6875 6876
Size: 697 12 64 1246 588 486 353 1
Cuts at: 6876 6997 7014 7098 7339 7665
Size: 121 17 84 241 326
Fragments arranged by size:
1246 1246 697 645 588 567 486 407
353 326 280 241 149 121 110 84
64 25 17 12 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4632 7665
Size: 4632 3033
FspI TGC'GCA
Cuts at: 0 3314 4756 4879 5560 6358 7665
Size: 3314 1442 123 681 798 1307
Fragments arranged by size:
3314 1442 1307 798 681 123
HaeII r_GCGC'y
Cuts at: 0 19 37 1025 1043 3789 3906 4028 5116
Size: 19 18 988 18 2746 117 122 1088
Cuts at: 5116 5280 5753 5838 6772 7073 7665
Size: 164 473 85 934 301 592
Fragments arranged by size:
2746 1088 988 934 592 473 301 164
122 117 85 19 18 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1231 1263 1334 1366 2257 2289 2371 2403
Size: 1231 32 71 32 891 32 82 32
Cuts at: 2403 3430 3462 3679 3711 3799 3831 4201 4233
Size: 1027 32 217 32 88 32 370 32
Cuts at: 4233 6625 6657 6916 6948 7543 7575 7665
Size: 2392 32 259 32 595 32 90
Fragments arranged by size:
2392 1231 1027 891 595 370 259 217
90 88 82 71 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 537 972 1359 1467 2289 2604 3330 3687
Size: 537 435 387 108 822 315 726 357
Cuts at: 3687 3804 5601 6063 7665
Size: 117 1797 462 1602
Fragments arranged by size:
1797 1602 822 726 537 462 435 387
357 315 117 108
KasI G'GCGC_C
Cuts at: 0 15 1039 3902 4024 5749 7665
Size: 15 1024 2863 122 1725 1916
Fragments arranged by size:
2863 1916 1725 1024 122 15
MluI A'CGCG_T
Cuts at: 0 4409 7665
Size: 4409 3256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1178 2300 2414 4001 5756 7604 7613
Size: 461 717 1122 114 1587 1755 1848 9
Cuts at: 7613 7665
Size: 52
Fragments arranged by size:
1848 1755 1587 1122 717 461 114 52
9
MscI TGG'CCA
Cuts at: 0 458 548 5294 7120 7665
Size: 458 90 4746 1826 545
Fragments arranged by size:
4746 1826 545 458 90
MslI CAynn'nnrTG
Cuts at: 0 725 1055 1432 1654 2992 3185 3730 4720
Size: 725 330 377 222 1338 193 545 990
Cuts at: 4720 4807 5092 5212 5543 7624 7665
Size: 87 285 120 331 2081 41
Fragments arranged by size:
2081 1338 990 725 545 377 331 330
285 222 193 120 87 41
MspA1I CmG'CkG
Cuts at: 0 263 383 608 845 900 1511 2108 2351
Size: 263 120 225 237 55 611 597 243
Cuts at: 2351 2675 2927 3245 3260 3395 3755 3908 3989
Size: 324 252 318 15 135 360 153 81
Cuts at: 3989 4329 4752 5015 5199 5551 5630 5672 5892
Size: 340 423 263 184 352 79 42 220
Cuts at: 5892 6369 6384 6869 7047 7277 7665
Size: 477 15 485 178 230 388
Fragments arranged by size:
611 597 485 477 423 388 360 352
340 324 318 263 263 252 243 237
230 225 220 184 178 153 135 120
81 79 55 42 15 15
NaeI GCC'GGC
Cuts at: 0 526 2635 2691 2734 3519 4536 4713 5192
Size: 526 2109 56 43 785 1017 177 479
Cuts at: 5192 5281 5720 6787 7665
Size: 89 439 1067 878
Fragments arranged by size:
2109 1067 1017 878 785 526 479 439
177 89 56 43
NarI GG'CG_CC
Cuts at: 0 16 1040 3903 4025 5750 7665
Size: 16 1024 2863 122 1725 1915
Fragments arranged by size:
2863 1915 1725 1024 122 16
NcoI C'CATG_G
Cuts at: 0 510 1086 5003 5570 6618 7665
Size: 510 576 3917 567 1048 1047
Fragments arranged by size:
3917 1048 1047 576 567 510
NdeI CA'TA_TG
Cuts at: 0 6275 7665
Size: 6275 1390
NgoMIV G'CCGG_C
Cuts at: 0 524 2633 2689 2732 3517 4534 4711 5190
Size: 524 2109 56 43 785 1017 177 479
Cuts at: 5190 5279 5718 6785 7665
Size: 89 439 1067 880
Fragments arranged by size:
2109 1067 1017 880 785 524 479 439
177 89 56 43
NotI GC'GGCC_GC
Cuts at: 0 5222 7665
Size: 5222 2443
NsiI A_TGCA'T
Cuts at: 0 1129 4090 5978 6317 7665
Size: 1129 2961 1888 339 1348
Fragments arranged by size:
2961 1888 1348 1129 339
NspI r_CATG'y
Cuts at: 0 221 1127 1276 1437 2022 4088 4693 5565
Size: 221 906 149 161 585 2066 605 872
Cuts at: 5565 5899 5976 6030 6315 6588 7629 7665
Size: 334 77 54 285 273 1041 36
Fragments arranged by size:
2066 1041 906 872 605 585 334 285
273 221 161 149 77 54 36
PasI CC'CwG_GG
Cuts at: 0 270 2227 3144 5589 7665
Size: 270 1957 917 2445 2076
Fragments arranged by size:
2445 2076 1957 917 270
PflMI CCAn_nnn'nTGG
Cuts at: 0 228 1236 2579 3263 4281 7665
Size: 228 1008 1343 684 1018 3384
Fragments arranged by size:
3384 1343 1018 1008 684 228
PfoI T'CCnGG_A
Cuts at: 0 783 3697 4280 5867 7665
Size: 783 2914 583 1587 1798
Fragments arranged by size:
2914 1798 1587 783 583
PmlI CAC'GTG
Cuts at: 0 225 2414 2474 2903 4031 7665
Size: 225 2189 60 429 1128 3634
Fragments arranged by size:
3634 2189 1128 429 225 60
PpuMI rG'GwC_Cy
Cuts at: 0 174 929 1903 3429 4202 5448 6036 6522
Size: 174 755 974 1526 773 1246 588 486
Cuts at: 6522 7665
Size: 1143
Fragments arranged by size:
1526 1246 1143 974 773 755 588 486
174
PshAI GACnn'nnGTC
Cuts at: 0 779 7665
Size: 779 6886
PspOMI G'GGCC_C
Cuts at: 0 159 283 1215 1335 4125 4137 6875 7338
Size: 159 124 932 120 2790 12 2738 463
Cuts at: 7338 7665
Size: 327
Fragments arranged by size:
2790 2738 932 463 327 159 124 120
12
PstI C_TGCA'G
Cuts at: 0 262 667 997 1228 1325 2515 4390 4397
Size: 262 405 330 231 97 1190 1875 7
Cuts at: 4397 4484 5671 6944 7028 7193 7226 7665
Size: 87 1187 1273 84 165 33 439
Fragments arranged by size:
1875 1273 1190 1187 439 405 330 262
231 165 97 87 84 33 7
PvuII CAG'CTG
Cuts at: 0 263 900 3755 4752 5199 6369 7665
Size: 263 637 2855 997 447 1170 1296
Fragments arranged by size:
2855 1296 1170 997 637 447 263
SacI G_AGCT'C
Cuts at: 0 2971 4787 7267 7665
Size: 2971 1816 2480 398
Fragments arranged by size:
2971 2480 1816 398
SacII CC_GC'GG
Cuts at: 0 3990 5016 5631 7665
Size: 3990 1026 615 2034
Fragments arranged by size:
3990 2034 1026 615
SalI G'TCGA_C
Cuts at: 0 2602 7665
Size: 2602 5063
ScaI AGT'ACT
Cuts at: 0 871 4495 4603 7665
Size: 871 3624 108 3062
Fragments arranged by size:
3624 3062 871 108
SexAI A'CCwGG_T
Cuts at: 0 3431 3737 6350 7133 7665
Size: 3431 306 2613 783 532
Fragments arranged by size:
3431 2613 783 532 306
SfcI C'TryA_G
Cuts at: 0 258 663 993 1224 1321 2511 2694 3126
Size: 258 405 330 231 97 1190 183 432
Cuts at: 3126 3750 4287 4386 4393 4480 5667 6940 7024
Size: 624 537 99 7 87 1187 1273 84
Cuts at: 7024 7189 7222 7504 7665
Size: 165 33 282 161
Fragments arranged by size:
1273 1190 1187 624 537 432 405 330
282 258 231 183 165 161 99 97
87 84 33 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 7286 7665
Size: 7286 379
SfoI GGC'GCC
Cuts at: 0 17 1041 3904 4026 5751 7665
Size: 17 1024 2863 122 1725 1914
Fragments arranged by size:
2863 1914 1725 1024 122 17
SgrAI Cr'CCGG_yG
Cuts at: 0 5190 5279 7665
Size: 5190 89 2386
Fragments arranged by size:
5190 2386 89
SmaI CCC'GGG
Cuts at: 0 558 6208 7665
Size: 558 5650 1457
Fragments arranged by size:
5650 1457 558
SmlI C'TyrA_G
Cuts at: 0 5380 6445 6526 7665
Size: 5380 1065 81 1139
Fragments arranged by size:
5380 1139 1065 81
SphI G_CATG'C
Cuts at: 0 1127 1276 1437 5899 5976 6030 6315 6588
Size: 1127 149 161 4462 77 54 285 273
Cuts at: 6588 7629 7665
Size: 1041 36
Fragments arranged by size:
4462 1127 1041 285 273 161 149 77
54 36
SrfI GCCC'GGGC
Cuts at: 0 558 7665
Size: 558 7107
StuI AGG'CCT
Cuts at: 0 133 478 4948 5062 6494 7657 7665
Size: 133 345 4470 114 1432 1163 8
Fragments arranged by size:
4470 1432 1163 345 133 114 8
StyI C'CwwG_G
Cuts at: 0 510 1086 5003 5570 6230 6518 6618 7254
Size: 510 576 3917 567 660 288 100 636
Cuts at: 7254 7665
Size: 411
Fragments arranged by size:
3917 660 636 576 567 510 411 288
100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 516 640 733 958 1243 2219 2674 2731
Size: 516 124 93 225 285 976 455 57
Cuts at: 2731 3250 3465 3769 3856 6733 6763 7138 7665
Size: 519 215 304 87 2877 30 375 527
Fragments arranged by size:
2877 976 527 519 516 455 375 304
285 225 215 124 93 87 57 30
TatI w'GTAC_w
Cuts at: 0 371 869 4493 4601 6344 7665
Size: 371 498 3624 108 1743 1321
Fragments arranged by size:
3624 1743 1321 498 371 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 361 393 491 523 1408 1440 1717 1749
Size: 361 32 98 32 885 32 277 32
Cuts at: 1749 2232 2264 2629 2661 2922 2954 4564 4596
Size: 483 32 365 32 261 32 1610 32
Cuts at: 4596 4648 4680 7453 7485 7665
Size: 52 32 2773 32 180
Fragments arranged by size:
2773 1610 885 483 365 361 277 261
180 98 52 32 32 32 32 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1747 7665
Size: 1747 5918
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2478 3151 5028 5838 6237 6432 6759 6852
Size: 2478 673 1877 810 399 195 327 93
Cuts at: 6852 7113 7281 7665
Size: 261 168 384
Fragments arranged by size:
2478 1877 810 673 399 384 327 261
195 168 93
XmaI C'CCGG_G
Cuts at: 0 556 6206 7665
Size: 556 5650 1459
Fragments arranged by size:
5650 1459 556
XmnI GAAnn'nnTTC
Cuts at: 0 4495 7665
Size: 4495 3170
ZraI GAC'GTC
Cuts at: 0 714 1356 5010 5619 6060 7665
Size: 714 642 3654 609 441 1605
Fragments arranged by size:
3654 1605 714 642 609 441
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KasI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI
NsiI NspI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PstI PvuII SacI SacII SalI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI SmlI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AloI AscI AseI AsiSI
AvrII BciVI BclI BglII BmtI BsiWI BsrDI BsrGI
BssHII BstBI BstEII BstZ17I DraI EcoRI EcoRV FseI
FspAI HindIII HpaI KpnI MfeI NheI NruI PacI
PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII
SanDI SapI SbfI SgfI SnaBI SpeI SspI SwaI
XbaI XhoI