Restriction map for
pF1KE3359
(Linear) (Six-base) MAPSORT of: KIEE3359.seq Check: 2983 from: 1 to: 3183
KIEE3359.orf
With 182 enzymes: SgfI *
September 19, 2014 17:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1577 2676 3183
Size: 1577 1099 507
Fragments arranged by size:
1577 1099 507
AccI GT'mk_AC
Cuts at: 0 2165 3183
Size: 2165 1018
Acc65I G'GTAC_C
Cuts at: 0 1337 3183
Size: 1337 1846
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 997 1242 1771 2034 2170 2602 2619
Size: 735 262 245 529 263 136 432 17
Cuts at: 2619 2980 3055 3183
Size: 361 75 128
Fragments arranged by size:
735 529 432 361 263 262 245 136
128 75 17
AfeI AGC'GCT
Cuts at: 0 545 2660 3183
Size: 545 2115 523
Fragments arranged by size:
2115 545 523
AflIII A'CryG_T
Cuts at: 0 1701 3183
Size: 1701 1482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2698 3183
Size: 2698 485
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 592 624 1885 1917 3183
Size: 592 32 1261 32 1266
Fragments arranged by size:
1266 1261 592 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 872 2556 2572 2935 3183
Size: 872 1684 16 363 248
Fragments arranged by size:
1684 872 363 248 16
ApaI G_GGCC'C
Cuts at: 0 11 346 479 856 3183
Size: 11 335 133 377 2327
Fragments arranged by size:
2327 377 335 133 11
ApoI r'AATT_y
Cuts at: 0 970 2407 3183
Size: 970 1437 776
Fragments arranged by size:
1437 970 776
AscI GG'CGCG_CC
Cuts at: 0 202 3183
Size: 202 2981
AvaI C'yCGr_G
Cuts at: 0 958 1243 2267 3176 3183
Size: 958 285 1024 909 7
Fragments arranged by size:
1024 958 909 285 7
BamHI G'GATC_C
Cuts at: 0 1239 3183
Size: 1239 1944
BanI G'GyrC_C
Cuts at: 0 14 418 1252 1337 1565 3183
Size: 14 404 834 85 228 1618
Fragments arranged by size:
1618 834 404 228 85 14
BanII G_rGCy'C
Cuts at: 0 11 346 479 856 1070 1550 2117 2149
Size: 11 335 133 377 214 480 567 32
Cuts at: 2149 2242 2798 3183
Size: 93 556 385
Fragments arranged by size:
567 556 480 385 377 335 214 133
93 32 11
BbeI G_GCGC'C
Cuts at: 0 18 422 3183
Size: 18 404 2761
Fragments arranged by size:
2761 404 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 1385 2205 3041 3183
Size: 1385 820 836 142
Fragments arranged by size:
1385 836 820 142
BbvCI CC'TCA_GC
Cuts at: 0 673 2354 2534 3183
Size: 673 1681 180 649
Fragments arranged by size:
1681 673 649 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1234 1268 3183
Size: 1234 34 1915
Fragments arranged by size:
1915 1234 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1174 2982 3183
Size: 1174 1808 201
Fragments arranged by size:
1808 1174 201
BglI GCCn_nnn'nGGC
Cuts at: 0 23 95 910 919 2842 3183
Size: 23 72 815 9 1923 341
Fragments arranged by size:
1923 815 341 72 23 9
BlpI GC'TnA_GC
Cuts at: 0 2573 3183
Size: 2573 610
Bme1580I G_kGCm'C
Cuts at: 0 11 346 479 856 906 3183
Size: 11 335 133 377 50 2277
Fragments arranged by size:
2277 377 335 133 50 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 364 1544 3183
Size: 364 1180 1639
Fragments arranged by size:
1639 1180 364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1435 1516 1891 2062 2338 2500 2536
Size: 673 762 81 375 171 276 162 36
Cuts at: 2536 2966 3091 3115 3183
Size: 430 125 24 68
Fragments arranged by size:
762 673 430 375 276 171 162 125
81 68 36 24
Bpu10I CC'TnA_GC
Cuts at: 0 673 1597 2111 2236 2354 2534 3183
Size: 673 924 514 125 118 180 649
Fragments arranged by size:
924 673 649 514 180 125 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1579 1966 2005 2991 3183
Size: 295 1284 387 39 986 192
Fragments arranged by size:
1284 986 387 295 192 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 3049 3183
Size: 3049 134
BsaAI yAC'GTr
Cuts at: 0 2220 3183
Size: 2220 963
BsaBI GATnn'nnATC
Cuts at: 0 1238 3183
Size: 1238 1945
BsaHI Gr'CG_yC
Cuts at: 0 15 269 419 3183
Size: 15 254 150 2764
Fragments arranged by size:
2764 254 150 15
BsaWI w'CCGG_w
Cuts at: 0 1904 3183
Size: 1904 1279
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 592 622 3183
Size: 592 30 2561
Fragments arranged by size:
2561 592 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1334 2217 2335 2509 2533 3088 3183
Size: 1334 883 118 174 24 555 95
Fragments arranged by size:
1334 883 555 174 118 95 24
BseYI C'CCAG_C
Cuts at: 0 243 264 429 478 492 525 530 1047
Size: 243 21 165 49 14 33 5 517
Cuts at: 1047 1383 1532 2348 3183
Size: 336 149 816 835
Fragments arranged by size:
835 816 517 336 243 165 149 49
33 21 14 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1598 1788 1789 2620 3183
Size: 499 1099 190 1 831 563
Fragments arranged by size:
1099 831 563 499 190 1
BsiEI CG_ry'CG
Cuts at: 0 568 627 1209 3183
Size: 568 59 582 1974
Fragments arranged by size:
1974 582 568 59
BsiHKAI G_wGCw'C
Cuts at: 0 323 584 2021 2117 2677 3183
Size: 323 261 1437 96 560 506
Fragments arranged by size:
1437 560 506 323 261 96
BsmI GAATG_Cn'
Cuts at: 0 1454 3183
Size: 1454 1729
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2587 3183
Size: 2587 596
Bsp1286I G_dGCh'C
Cuts at: 0 11 323 346 479 584 856 906 1070
Size: 11 312 23 133 105 272 50 164
Cuts at: 1070 1550 2021 2117 2149 2242 2677 2798 3183
Size: 480 471 96 32 93 435 121 385
Fragments arranged by size:
480 471 435 385 312 272 164 133
121 105 96 93 50 32 23 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1577 2453 2676 3183
Size: 1577 876 223 507
Fragments arranged by size:
1577 876 507 223
BsrBI CCG'CTC
Cuts at: 0 1601 2259 2499 3183
Size: 1601 658 240 684
Fragments arranged by size:
1601 684 658 240
BsrFI r'CCGG_y
Cuts at: 0 415 563 1586 2888 3183
Size: 415 148 1023 1302 295
Fragments arranged by size:
1302 1023 415 295 148
BsrGI T'GTAC_A
Cuts at: 0 2711 2750 3183
Size: 2711 39 433
Fragments arranged by size:
2711 433 39
BssHII G'CGCG_C
Cuts at: 0 157 202 3183
Size: 157 45 2981
Fragments arranged by size:
2981 157 45
BssSI C'ACGA_G
Cuts at: 0 595 1687 3183
Size: 595 1092 1496
Fragments arranged by size:
1496 1092 595
BstAPI GCAn_nnn'nTGC
Cuts at: 0 837 3183
Size: 837 2346
BstEII G'GTnAC_C
Cuts at: 0 2691 3183
Size: 2691 492
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1794 1998 2688 3183
Size: 1794 204 690 495
Fragments arranged by size:
1794 690 495 204
BstYI r'GATC_y
Cuts at: 0 1239 2387 2552 3038 3183
Size: 1239 1148 165 486 145
Fragments arranged by size:
1239 1148 486 165 145
BstZ17I GTA'TAC
Cuts at: 0 2166 3183
Size: 2166 1017
Bsu36I CC'TnA_GG
Cuts at: 0 2200 3183
Size: 2200 983
BtgI C'CryG_G
Cuts at: 0 113 173 299 1019 1848 1971 3183
Size: 113 60 126 720 829 123 1212
Fragments arranged by size:
1212 829 720 126 123 113 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2786 3183
Size: 2786 397
BtsI GCAGTG_nn'
Cuts at: 0 582 1094 1206 2851 3183
Size: 582 512 112 1645 332
Fragments arranged by size:
1645 582 512 332 112
DraIII CAC_nnn'GTG
Cuts at: 0 2568 3183
Size: 2568 615
EaeI y'GGCC_r
Cuts at: 0 128 296 565 3183
Size: 128 168 269 2618
Fragments arranged by size:
2618 269 168 128
EagI C'GGCC_G
Cuts at: 0 565 3183
Size: 565 2618
EarI CTCTTCn'nnn_
Cuts at: 0 572 3183
Size: 572 2611
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2380 2683 2872 3183
Size: 2380 303 189 311
Fragments arranged by size:
2380 311 303 189
EcoICRI GAG'CTC
Cuts at: 0 2115 3183
Size: 2115 1068
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 735 997 1242 1435 1516 1771 1891
Size: 673 62 262 245 193 81 255 120
Cuts at: 1891 2034 2062 2170 2338 2500 2536 2602 2619
Size: 143 28 108 168 162 36 66 17
Cuts at: 2619 2966 2980 3055 3091 3115 3183
Size: 347 14 75 36 24 68
Fragments arranged by size:
673 347 262 255 245 193 168 162
143 120 108 81 75 68 66 62
36 36 28 24 17 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 190 598 3183
Size: 190 408 2585
Fragments arranged by size:
2585 408 190
EcoO109I rG'GnC_Cy
Cuts at: 0 7 8 166 343 399 475 852 853
Size: 7 1 158 177 56 76 377 1
Cuts at: 853 869 2055 2358 2538 2932 3183
Size: 16 1186 303 180 394 251
Fragments arranged by size:
1186 394 377 303 251 180 177 158
76 56 16 7 1 1
EcoRI G'AATT_C
Cuts at: 0 970 3183
Size: 970 2213
EcoRV GAT'ATC
Cuts at: 0 822 3183
Size: 822 2361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1420 3060 3183
Size: 1420 1640 123
Fragments arranged by size:
1640 1420 123
FspI TGC'GCA
Cuts at: 0 2404 2914 3183
Size: 2404 510 269
Fragments arranged by size:
2404 510 269
HaeII r_GCGC'y
Cuts at: 0 18 292 381 422 430 514 540 547
Size: 18 274 89 41 8 84 26 7
Cuts at: 547 2013 2662 3183
Size: 1466 649 521
Fragments arranged by size:
1466 649 521 274 89 84 41 26
18 8 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1083 1115 2544 2576 3183
Size: 1083 32 1429 32 607
Fragments arranged by size:
1429 1083 607 32 32
HincII GTy'rAC
Cuts at: 0 2997 3183
Size: 2997 186
HindIII A'AGCT_T
Cuts at: 0 1161 3183
Size: 1161 2022
KasI G'GCGC_C
Cuts at: 0 14 418 3183
Size: 14 404 2765
Fragments arranged by size:
2765 404 14
KpnI G_GTAC'C
Cuts at: 0 1341 3183
Size: 1341 1842
MfeI C'AATT_G
Cuts at: 0 2765 3183
Size: 2765 418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 2015 3183
Size: 302 1713 1168
Fragments arranged by size:
1713 1168 302
MscI TGG'CCA
Cuts at: 0 130 3183
Size: 130 3053
MslI CAynn'nnrTG
Cuts at: 0 2680 2686 2785 3183
Size: 2680 6 99 398
Fragments arranged by size:
2680 398 99 6
MspA1I CmG'CkG
Cuts at: 0 22 66 126 301 331 405 1146 1494
Size: 22 44 60 175 30 74 741 348
Cuts at: 1494 2343 2580 2904 3183
Size: 849 237 324 279
Fragments arranged by size:
849 741 348 324 279 237 175 74
60 44 30 22
NaeI GCC'GGC
Cuts at: 0 417 1588 3183
Size: 417 1171 1595
Fragments arranged by size:
1595 1171 417
NarI GG'CG_CC
Cuts at: 0 15 419 3183
Size: 15 404 2764
Fragments arranged by size:
2764 404 15
NcoI C'CATG_G
Cuts at: 0 1019 1971 3183
Size: 1019 952 1212
Fragments arranged by size:
1212 1019 952
NdeI CA'TA_TG
Cuts at: 0 1757 3183
Size: 1757 1426
NgoMIV G'CCGG_C
Cuts at: 0 415 1586 3183
Size: 415 1171 1597
Fragments arranged by size:
1597 1171 415
NspI r_CATG'y
Cuts at: 0 1391 1941 3183
Size: 1391 550 1242
Fragments arranged by size:
1391 1242 550
PasI CC'CwG_GG
Cuts at: 0 872 1332 3183
Size: 872 460 1851
Fragments arranged by size:
1851 872 460
PflMI CCAn_nnn'nTGG
Cuts at: 0 1296 3183
Size: 1296 1887
PfoI T'CCnGG_A
Cuts at: 0 2435 3068 3183
Size: 2435 633 115
Fragments arranged by size:
2435 633 115
PmlI CAC'GTG
Cuts at: 0 2220 3183
Size: 2220 963
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 1630 1662 3183
Size: 592 32 1006 32 1521
Fragments arranged by size:
1521 1006 592 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 166 399 2055 2358 2932 3183
Size: 166 233 1656 303 574 251
Fragments arranged by size:
1656 574 303 251 233 166
PsiI TTA'TAA
Cuts at: 0 1676 3183
Size: 1676 1507
PspOMI G'GGCC_C
Cuts at: 0 7 342 475 852 3183
Size: 7 335 133 377 2331
Fragments arranged by size:
2331 377 335 133 7
PstI C_TGCA'G
Cuts at: 0 736 1862 2309 3183
Size: 736 1126 447 874
Fragments arranged by size:
1126 874 736 447
PvuI CG_AT'CG
Cuts at: 0 1209 3183
Size: 1209 1974
PvuII CAG'CTG
Cuts at: 0 1494 2580 2904 3183
Size: 1494 1086 324 279
Fragments arranged by size:
1494 1086 324 279
RsrII CG'GwC_CG
Cuts at: 0 231 3183
Size: 231 2952
SacI G_AGCT'C
Cuts at: 0 2117 3183
Size: 2117 1066
SacII CC_GC'GG
Cuts at: 0 302 3183
Size: 302 2881
SanDI GG'GwC_CC
Cuts at: 0 166 399 3183
Size: 166 233 2784
Fragments arranged by size:
2784 233 166
SapI GCTCTTCn'nnn_
Cuts at: 0 572 3183
Size: 572 2611
SbfI CC_TGCA'GG
Cuts at: 0 1862 3183
Size: 1862 1321
ScaI AGT'ACT
Cuts at: 0 964 1840 3183
Size: 964 876 1343
Fragments arranged by size:
1343 964 876
SexAI A'CCwGG_T
Cuts at: 0 1786 3183
Size: 1786 1397
SfcI C'TryA_G
Cuts at: 0 732 1858 2305 3183
Size: 732 1126 447 878
Fragments arranged by size:
1126 878 732 447
SfoI GGC'GCC
Cuts at: 0 16 420 3183
Size: 16 404 2763
Fragments arranged by size:
2763 404 16
SgrAI Cr'CCGG_yG
Cuts at: 0 415 3183
Size: 415 2768
SmlI C'TyrA_G
Cuts at: 0 310 1558 1981 2020 3006 3183
Size: 310 1248 423 39 986 177
Fragments arranged by size:
1248 986 423 310 177 39
SphI G_CATG'C
Cuts at: 0 1391 1941 3183
Size: 1391 550 1242
Fragments arranged by size:
1391 1242 550
SspI AAT'ATT
Cuts at: 0 636 3183
Size: 636 2547
StuI AGG'CCT
Cuts at: 0 913 3183
Size: 913 2270
StyI C'CwwG_G
Cuts at: 0 1019 1971 3059 3153 3183
Size: 1019 952 1088 94 30
Fragments arranged by size:
1088 1019 952 94 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2694 3183
Size: 2694 489
TatI w'GTAC_w
Cuts at: 0 962 1266 1838 2711 2750 3183
Size: 962 304 572 873 39 433
Fragments arranged by size:
962 873 572 433 304 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 298 330 1721 1753 3183
Size: 158 32 108 32 1391 32 1430
Fragments arranged by size:
1430 1391 158 108 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1927 3183
Size: 1927 1256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3066 3183
Size: 3066 117
XmnI GAAnn'nnTTC
Cuts at: 0 2127 3183
Size: 2127 1056
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AlfI AloI
AlwNI ApaI ApoI AscI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgrAI SmlI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AleI ApaLI AseI
AsiSI AvrII BaeI BclI BfrBI BglII BmgBI BmtI
BplI BsiWI BspEI BspHI BsrDI BstBI ClaI CspCI
DraI DrdI FseI FspAI HpaI MluI NheI NotI
NruI NsiI PacI PciI PmeI PshAI PspXI PsrI
SalI SfiI SgfI SmaI SnaBI SpeI SrfI SwaI
XbaI XhoI XmaI ZraI