Restriction map for
pF1KE3369
(Linear) (Six-base) MAPSORT of: KIEE3369.seq Check: 5301 from: 1 to: 3267
KIEE3369.orf
With 182 enzymes: SgfI *
November 11, 2016 16:52 ..
AccI GT'mk_AC
Cuts at: 0 2774 3267
Size: 2774 493
Acc65I G'GTAC_C
Cuts at: 0 501 2735 3267
Size: 501 2234 532
Fragments arranged by size:
2234 532 501
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 945 1331 1561 1954 2929 3019 3155
Size: 206 739 386 230 393 975 90 136
Cuts at: 3155 3224 3267
Size: 69 43
Fragments arranged by size:
975 739 393 386 230 206 136 90
69 43
AfeI AGC'GCT
Cuts at: 0 3039 3267
Size: 3039 228
AleI CACnn'nnGTG
Cuts at: 0 488 1096 2837 3267
Size: 488 608 1741 430
Fragments arranged by size:
1741 608 488 430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 793 3267
Size: 793 2474
AlwNI CAG_nnn'CTG
Cuts at: 0 3033 3267
Size: 3033 234
ApaI G_GGCC'C
Cuts at: 0 557 3267
Size: 557 2710
ApaLI G'TGCA_C
Cuts at: 0 1302 3267
Size: 1302 1965
ApoI r'AATT_y
Cuts at: 0 123 1667 2891 3267
Size: 123 1544 1224 376
Fragments arranged by size:
1544 1224 376 123
AvaI C'yCGr_G
Cuts at: 0 463 832 1433 2408 3267
Size: 463 369 601 975 859
Fragments arranged by size:
975 859 601 463 369
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1428 1461 3267
Size: 1428 33 1806
Fragments arranged by size:
1806 1428 33
BanI G'GyrC_C
Cuts at: 0 501 2677 2735 3267
Size: 501 2176 58 532
Fragments arranged by size:
2176 532 501 58
BanII G_rGCy'C
Cuts at: 0 199 557 3267
Size: 199 358 2710
Fragments arranged by size:
2710 358 199
BbsI GAAGACnn'nnnn_
Cuts at: 0 31 1166 2996 3209 3260 3267
Size: 31 1135 1830 213 51 7
Fragments arranged by size:
1830 1135 213 51 31 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 853 887 1719 1753 3267
Size: 853 34 832 34 1514
Fragments arranged by size:
1514 853 832 34 34
BclI T'GATC_A
Cuts at: 0 1266 1613 3267
Size: 1266 347 1654
Fragments arranged by size:
1654 1266 347
BfrBI ATG'CAT
Cuts at: 0 2532 2959 3267
Size: 2532 427 308
Fragments arranged by size:
2532 427 308
BglI GCCn_nnn'nGGC
Cuts at: 0 274 880 3267
Size: 274 606 2387
Fragments arranged by size:
2387 606 274
BglII A'GATC_T
Cuts at: 0 2498 2660 3267
Size: 2498 162 607
Fragments arranged by size:
2498 607 162
BlpI GC'TnA_GC
Cuts at: 0 52 3267
Size: 52 3215
Bme1580I G_kGCm'C
Cuts at: 0 557 1306 3267
Size: 557 749 1961
Fragments arranged by size:
1961 749 557
BmrI ACTGGGnnnn_n'
Cuts at: 0 1292 3015 3267
Size: 1292 1723 252
Fragments arranged by size:
1723 1292 252
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1446 3267
Size: 1446 1821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1695 1823 3267
Size: 770 925 128 1444
Fragments arranged by size:
1444 925 770 128
Bpu10I CC'TnA_GC
Cuts at: 0 1151 2080 3267
Size: 1151 929 1187
Fragments arranged by size:
1187 1151 929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1217 1734 3267
Size: 1217 517 1533
Fragments arranged by size:
1533 1217 517
BsaI GGTCTCn'nnnn_
Cuts at: 0 1477 2811 3187 3267
Size: 1477 1334 376 80
Fragments arranged by size:
1477 1334 376 80
BsaBI GATnn'nnATC
Cuts at: 0 1612 3267
Size: 1612 1655
BsaWI w'CCGG_w
Cuts at: 0 2738 3267
Size: 2738 529
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2030 2060 3267
Size: 2030 30 1207
Fragments arranged by size:
2030 1207 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1495 3103 3106 3267
Size: 1495 1608 3 161
Fragments arranged by size:
1608 1495 161 3
BseYI C'CCAG_C
Cuts at: 0 1980 3267
Size: 1980 1287
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 1974 3267
Size: 136 1838 1293
Fragments arranged by size:
1838 1293 136
BsiHKAI G_wGCw'C
Cuts at: 0 199 1306 3267
Size: 199 1107 1961
Fragments arranged by size:
1961 1107 199
BsmI GAATG_Cn'
Cuts at: 0 2645 3267
Size: 2645 622
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3145 3267
Size: 3145 122
Bsp1286I G_dGCh'C
Cuts at: 0 199 557 1306 3267
Size: 199 358 749 1961
Fragments arranged by size:
1961 749 358 199
BspHI T'CATG_A
Cuts at: 0 1862 3267
Size: 1862 1405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 513 992 3267
Size: 513 479 2275
Fragments arranged by size:
2275 513 479
BsrFI r'CCGG_y
Cuts at: 0 1847 3267
Size: 1847 1420
BsrGI T'GTAC_A
Cuts at: 0 290 815 3267
Size: 290 525 2452
Fragments arranged by size:
2452 525 290
BstBI TT'CG_AA
Cuts at: 0 2539 3267
Size: 2539 728
BstEII G'GTnAC_C
Cuts at: 0 696 3267
Size: 696 2571
BstXI CCAn_nnnn'nTGG
Cuts at: 0 810 1098 3267
Size: 810 288 2169
Fragments arranged by size:
2169 810 288
BstYI r'GATC_y
Cuts at: 0 642 1424 1900 2498 2660 3267
Size: 642 782 476 598 162 607
Fragments arranged by size:
782 642 607 598 476 162
Bsu36I CC'TnA_GG
Cuts at: 0 3086 3267
Size: 3086 181
BtgI C'CryG_G
Cuts at: 0 839 1272 1448 1892 3267
Size: 839 433 176 444 1375
Fragments arranged by size:
1375 839 444 433 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1473 1512 3267
Size: 1473 39 1755
Fragments arranged by size:
1755 1473 39
BtsI GCAGTG_nn'
Cuts at: 0 270 1880 3267
Size: 270 1610 1387
Fragments arranged by size:
1610 1387 270
DraI TTT'AAA
Cuts at: 0 623 3267
Size: 623 2644
DraIII CAC_nnn'GTG
Cuts at: 0 2626 3267
Size: 2626 641
DrdI GACnn_nn'nnGTC
Cuts at: 0 2329 3267
Size: 2329 938
EaeI y'GGCC_r
Cuts at: 0 1167 1394 1895 2516 2744 3267
Size: 1167 227 501 621 228 523
Fragments arranged by size:
1167 621 523 501 228 227
EarI CTCTTCn'nnn_
Cuts at: 0 185 317 1201 2809 2905 3131 3243 3267
Size: 185 132 884 1608 96 226 112 24
Fragments arranged by size:
1608 884 226 185 132 112 96 24
EcoICRI GAG'CTC
Cuts at: 0 197 3267
Size: 197 3070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 770 945 1331 1561 1695 1823 1954
Size: 206 564 175 386 230 134 128 131
Cuts at: 1954 2929 3019 3155 3224 3267
Size: 975 90 136 69 43
Fragments arranged by size:
975 564 386 230 206 175 136 134
131 128 90 69 43
EcoO109I rG'GnC_Cy
Cuts at: 0 539 553 554 2002 3096 3267
Size: 539 14 1 1448 1094 171
Fragments arranged by size:
1448 1094 539 171 14 1
EcoRI G'AATT_C
Cuts at: 0 123 3267
Size: 123 3144
EcoRV GAT'ATC
Cuts at: 0 1040 2118 3267
Size: 1040 1078 1149
Fragments arranged by size:
1149 1078 1040
HaeII r_GCGC'y
Cuts at: 0 3041 3267
Size: 3041 226
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 1059 1091 1929 1961 2030 2062 2341
Size: 29 1030 32 838 32 69 32 279
Cuts at: 2341 2373 2620 2652 2904 2936 3267
Size: 32 247 32 252 32 331
Fragments arranged by size:
1030 838 331 279 252 247 69 32
32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 0 729 981 1229 2049 3267
Size: 729 252 248 820 1218
Fragments arranged by size:
1218 820 729 252 248
HpaI GTT'AAC
Cuts at: 0 1229 3267
Size: 1229 2038
KpnI G_GTAC'C
Cuts at: 0 505 2739 3267
Size: 505 2234 528
Fragments arranged by size:
2234 528 505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 2288 3267
Size: 950 1338 979
Fragments arranged by size:
1338 979 950
MscI TGG'CCA
Cuts at: 0 1169 1396 2518 2746 3267
Size: 1169 227 1122 228 521
Fragments arranged by size:
1169 1122 521 228 227
MslI CAynn'nnrTG
Cuts at: 0 358 488 775 1096 1492 2197 2698 2837
Size: 358 130 287 321 396 705 501 139
Cuts at: 2837 2941 3211 3267
Size: 104 270 56
Fragments arranged by size:
705 501 396 358 321 287 270 139
130 104 56
MspA1I CmG'CkG
Cuts at: 0 120 233 1309 1737 3267
Size: 120 113 1076 428 1530
Fragments arranged by size:
1530 1076 428 120 113
NcoI C'CATG_G
Cuts at: 0 1272 3267
Size: 1272 1995
NsiI A_TGCA'T
Cuts at: 0 2534 2961 3267
Size: 2534 427 306
Fragments arranged by size:
2534 427 306
NspI r_CATG'y
Cuts at: 0 2940 3267
Size: 2940 327
PflMI CCAn_nnn'nTGG
Cuts at: 0 1771 3267
Size: 1771 1496
PfoI T'CCnGG_A
Cuts at: 0 1381 3267
Size: 1381 1886
PpuMI rG'GwC_Cy
Cuts at: 0 539 3096 3267
Size: 539 2557 171
Fragments arranged by size:
2557 539 171
PspOMI G'GGCC_C
Cuts at: 0 553 3267
Size: 553 2714
PstI C_TGCA'G
Cuts at: 0 1590 3267
Size: 1590 1677
PvuII CAG'CTG
Cuts at: 0 120 1309 1737 3267
Size: 120 1189 428 1530
Fragments arranged by size:
1530 1189 428 120
SacI G_AGCT'C
Cuts at: 0 199 3267
Size: 199 3068
SapI GCTCTTCn'nnn_
Cuts at: 0 1201 3267
Size: 1201 2066
SexAI A'CCwGG_T
Cuts at: 0 3245 3267
Size: 3245 22
SfcI C'TryA_G
Cuts at: 0 548 1586 3267
Size: 548 1038 1681
Fragments arranged by size:
1681 1038 548
SmlI C'TyrA_G
Cuts at: 0 1232 1749 3267
Size: 1232 517 1518
Fragments arranged by size:
1518 1232 517
SpeI A'CTAG_T
Cuts at: 0 1776 3267
Size: 1776 1491
SphI G_CATG'C
Cuts at: 0 2940 3267
Size: 2940 327
SspI AAT'ATT
Cuts at: 0 2260 3267
Size: 2260 1007
StuI AGG'CCT
Cuts at: 0 2366 3267
Size: 2366 901
StyI C'CwwG_G
Cuts at: 0 1272 1518 2670 3267
Size: 1272 246 1152 597
Fragments arranged by size:
1272 1152 597 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2105 3267
Size: 2105 1162
TatI w'GTAC_w
Cuts at: 0 290 815 3267
Size: 290 525 2452
Fragments arranged by size:
2452 525 290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 444 476 1280 1308 1312 1340 1613 1645
Size: 444 32 804 28 4 28 273 32
Cuts at: 1645 2210 2242 2477 2509 3267
Size: 565 32 235 32 758
Fragments arranged by size:
804 758 565 444 273 235 32 32
32 32 28 28 4
Tth111I GACn'n_nGTC
Cuts at: 0 1258 3079 3267
Size: 1258 1821 188
Fragments arranged by size:
1821 1258 188
XbaI T'CTAG_A
Cuts at: 0 645 3267
Size: 645 2622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1254 1279 3267
Size: 1254 25 1988
Fragments arranged by size:
1988 1254 25
XmnI GAAnn'nnTTC
Cuts at: 0 937 1106 2891 3267
Size: 937 169 1785 376
Fragments arranged by size:
1785 937 376 169
Enzymes that do cut:
AccI Acc65I AcuI AfeI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BaeI BanI BanII BbsI BcgI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrFI BsrGI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HpaI
KpnI MmeI MscI MslI MspA1I NcoI NsiI NspI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI
SexAI SfcI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AloI
AscI AseI AsiSI AvrII BamHI BbeI BbvCI BciVI
BmgBI BmtI BsaAI BsaHI BsiEI BsiWI BspEI BsrBI
BsrDI BssHII BssSI BstAPI BstZ17I ClaI CspCI EagI
EciI EcoNI FalI FseI FspI FspAI HindIII KasI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI SwaI XhoI XmaI ZraI