Restriction map for
pF1KE3374
(Linear) (Six-base) MAPSORT of: KIEE3374.seq Check: 8272 from: 1 to: 3351
KIEE3374.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 503 2274 2349 3351
Size: 503 1771 75 1002
Fragments arranged by size:
1771 1002 503 75
AatII G_ACGT'C
Cuts at: 0 2269 3351
Size: 2269 1082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 1693 1902 3351
Size: 952 741 209 1449
Fragments arranged by size:
1449 952 741 209
AflIII A'CryG_T
Cuts at: 0 878 3351
Size: 878 2473
AgeI A'CCGG_T
Cuts at: 0 1886 3351
Size: 1886 1465
AhdI GACnn_n'nnGTC
Cuts at: 0 2315 3351
Size: 2315 1036
AleI CACnn'nnGTG
Cuts at: 0 646 2428 2593 3351
Size: 646 1782 165 758
Fragments arranged by size:
1782 758 646 165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 652 2425 2936 3061 3351
Size: 652 1773 511 125 290
Fragments arranged by size:
1773 652 511 290 125
AlwNI CAG_nnn'CTG
Cuts at: 0 46 309 453 759 1858 2124 2165 2697
Size: 46 263 144 306 1099 266 41 532
Cuts at: 2697 2991 3351
Size: 294 360
Fragments arranged by size:
1099 532 360 306 294 266 263 144
46 41
ApaI G_GGCC'C
Cuts at: 0 201 303 789 1565 3351
Size: 201 102 486 776 1786
Fragments arranged by size:
1786 776 486 201 102
ApaLI G'TGCA_C
Cuts at: 0 314 976 1594 3351
Size: 314 662 618 1757
Fragments arranged by size:
1757 662 618 314
ApoI r'AATT_y
Cuts at: 0 1011 1984 3351
Size: 1011 973 1367
Fragments arranged by size:
1367 1011 973
AvaI C'yCGr_G
Cuts at: 0 437 1846 1964 2242 3351
Size: 437 1409 118 278 1109
Fragments arranged by size:
1409 1109 437 278 118
BamHI G'GATC_C
Cuts at: 0 1298 1607 1643 3351
Size: 1298 309 36 1708
Fragments arranged by size:
1708 1298 309 36
BanI G'GyrC_C
Cuts at: 0 36 146 409 442 554 616 628 668
Size: 36 110 263 33 112 62 12 40
Cuts at: 668 1079 1876 2173 2484 3351
Size: 411 797 297 311 867
Fragments arranged by size:
867 797 411 311 297 263 112 110
62 40 36 33 12
BanII G_rGCy'C
Cuts at: 0 201 303 789 1565 1841 2219 2375 2624
Size: 201 102 486 776 276 378 156 249
Cuts at: 2624 2660 2763 3351
Size: 36 103 588
Fragments arranged by size:
776 588 486 378 276 249 201 156
103 102 36
BbeI G_GCGC'C
Cuts at: 0 150 446 558 672 1880 3351
Size: 150 296 112 114 1208 1471
Fragments arranged by size:
1471 1208 296 150 114 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 1533 1896 3229 3351
Size: 1533 363 1333 122
Fragments arranged by size:
1533 1333 363 122
BbvCI CC'TCA_GC
Cuts at: 0 559 3351
Size: 559 2792
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 935 969 2385 2419 3351
Size: 935 34 1416 34 932
Fragments arranged by size:
1416 935 932 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 542 599 1109 2223 3351
Size: 542 57 510 1114 1128
Fragments arranged by size:
1128 1114 542 510 57
BlpI GC'TnA_GC
Cuts at: 0 1835 3351
Size: 1835 1516
Bme1580I G_kGCm'C
Cuts at: 0 201 236 303 318 789 980 1565 1598
Size: 201 35 67 15 471 191 585 33
Cuts at: 1598 2176 2590 2634 3351
Size: 578 414 44 717
Fragments arranged by size:
717 585 578 471 414 201 191 67
44 35 33 15
BmgBI CAC'GTC
Cuts at: 0 1941 3351
Size: 1941 1410
BmrI ACTGGGnnnn_n'
Cuts at: 0 1095 1749 2127 3351
Size: 1095 654 378 1224
Fragments arranged by size:
1224 1095 654 378
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1272 3351
Size: 1272 2079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 138 151 207 732 736 852 1021
Size: 68 70 13 56 525 4 116 169
Cuts at: 1021 1032 1425 1597 1969 2632 2690 2968 3316
Size: 11 393 172 372 663 58 278 348
Cuts at: 3316 3351
Size: 35
Fragments arranged by size:
663 525 393 372 348 278 172 169
116 70 68 58 56 35 13 11
4
Bpu10I CC'TnA_GC
Cuts at: 0 559 1549 2822 3269 3351
Size: 559 990 1273 447 82
Fragments arranged by size:
1273 990 559 447 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2548 2918 3351
Size: 2548 370 433
Fragments arranged by size:
2548 433 370
BsaI GGTCTCn'nnnn_
Cuts at: 0 865 1401 3136 3351
Size: 865 536 1735 215
Fragments arranged by size:
1735 865 536 215
BsaAI yAC'GTr
Cuts at: 0 1788 3351
Size: 1788 1563
BsaBI GATnn'nnATC
Cuts at: 0 2809 3351
Size: 2809 542
BsaHI Gr'CG_yC
Cuts at: 0 102 147 443 555 669 1101 1877 1934
Size: 102 45 296 112 114 432 776 57
Cuts at: 1934 2266 2723 3351
Size: 332 457 628
Fragments arranged by size:
776 628 457 432 332 296 114 112
102 57 45
BsaWI w'CCGG_w
Cuts at: 0 815 1886 3351
Size: 815 1071 1465
Fragments arranged by size:
1465 1071 815
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 117 120 367 370 477 1261 1408 1807
Size: 117 3 247 3 107 784 147 399
Cuts at: 1807 3351
Size: 1544
Fragments arranged by size:
1544 784 399 247 147 117 107 3
3
BseYI C'CCAG_C
Cuts at: 0 67 115 161 302 446 1260 1820 2219
Size: 67 48 46 141 144 814 560 399
Cuts at: 2219 2583 2829 3001 3351
Size: 364 246 172 350
Fragments arranged by size:
814 560 399 364 350 246 172 144
141 67 48 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 813 2377 2449 2452 2527 2909 2977
Size: 523 290 1564 72 3 75 382 68
Cuts at: 2977 3031 3351
Size: 54 320
Fragments arranged by size:
1564 523 382 320 290 75 72 68
54 3
BsiEI CG_ry'CG
Cuts at: 0 698 1887 2757 3351
Size: 698 1189 870 594
Fragments arranged by size:
1189 870 698 594
BsiHKAI G_wGCw'C
Cuts at: 0 275 318 860 980 1258 1598 2204 2333
Size: 275 43 542 120 278 340 606 129
Cuts at: 2333 2624 2771 3351
Size: 291 147 580
Fragments arranged by size:
606 580 542 340 291 278 275 147
129 120 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3017 3334 3351
Size: 3017 317 17
Fragments arranged by size:
3017 317 17
Bsp1286I G_dGCh'C
Cuts at: 0 201 236 275 303 318 789 860 980
Size: 201 35 39 28 15 471 71 120
Cuts at: 980 1258 1565 1598 1841 2176 2204 2219 2333
Size: 278 307 33 243 335 28 15 114
Cuts at: 2333 2375 2590 2624 2634 2660 2763 2771 3351
Size: 42 215 34 10 26 103 8 580
Fragments arranged by size:
580 471 335 307 278 243 215 201
120 114 103 71 42 39 35 34
33 28 28 26 15 15 10 8
BspEI T'CCGG_A
Cuts at: 0 815 3351
Size: 815 2536
BspHI T'CATG_A
Cuts at: 0 1442 3218 3351
Size: 1442 1776 133
Fragments arranged by size:
1776 1442 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 482 503 1610 2274 2349 3098 3351
Size: 482 21 1107 664 75 749 253
Fragments arranged by size:
1107 749 664 482 253 75 21
BsrBI CCG'CTC
Cuts at: 0 152 807 1440 2304 3351
Size: 152 655 633 864 1047
Fragments arranged by size:
1047 864 655 633 152
BsrFI r'CCGG_y
Cuts at: 0 215 625 1886 2481 3302 3351
Size: 215 410 1261 595 821 49
Fragments arranged by size:
1261 821 595 410 215 49
BssSI C'ACGA_G
Cuts at: 0 1188 1957 3351
Size: 1188 769 1394
Fragments arranged by size:
1394 1188 769
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1602 2484 3185 3351
Size: 1602 882 701 166
Fragments arranged by size:
1602 882 701 166
BstXI CCAn_nnnn'nTGG
Cuts at: 0 75 589 2064 3351
Size: 75 514 1475 1287
Fragments arranged by size:
1475 1287 514 75
BstYI r'GATC_y
Cuts at: 0 812 1298 1607 1643 1775 2114 3113 3351
Size: 812 486 309 36 132 339 999 238
Fragments arranged by size:
999 812 486 339 309 238 132 36
Bsu36I CC'TnA_GG
Cuts at: 0 576 612 1391 1859 3351
Size: 576 36 779 468 1492
Fragments arranged by size:
1492 779 576 468 36
BtgI C'CryG_G
Cuts at: 0 293 503 1277 2426 3351
Size: 293 210 774 1149 925
Fragments arranged by size:
1149 925 774 293 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2388 3351
Size: 2388 963
BtsI GCAGTG_nn'
Cuts at: 0 2320 2729 2908 3351
Size: 2320 409 179 443
Fragments arranged by size:
2320 443 409 179
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1725 1760 3351
Size: 1725 35 1591
Fragments arranged by size:
1725 1591 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 879 1939 3351
Size: 879 1060 1412
Fragments arranged by size:
1412 1060 879
EaeI y'GGCC_r
Cuts at: 0 695 1175 2066 2273 3284 3351
Size: 695 480 891 207 1011 67
Fragments arranged by size:
1011 891 695 480 207 67
EagI C'GGCC_G
Cuts at: 0 695 3351
Size: 695 2656
EarI CTCTTCn'nnn_
Cuts at: 0 600 1345 2936 3351
Size: 600 745 1591 415
Fragments arranged by size:
1591 745 600 415
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 761 2306 2463 3351
Size: 761 1545 157 888
Fragments arranged by size:
1545 888 761 157
EcoICRI GAG'CTC
Cuts at: 0 2622 3351
Size: 2622 729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 138 151 207 732 736 852 952
Size: 68 70 13 56 525 4 116 100
Cuts at: 952 1021 1032 1425 1597 1693 1902 1969 2632
Size: 69 11 393 172 96 209 67 663
Cuts at: 2632 2690 2968 3316 3351
Size: 58 278 348 35
Fragments arranged by size:
663 525 393 348 278 209 172 116
100 96 70 69 68 67 58 56
35 13 11 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1365 1552 3351
Size: 1365 187 1799
Fragments arranged by size:
1799 1365 187
EcoO109I rG'GnC_Cy
Cuts at: 0 43 197 299 534 785 786 1316 1387
Size: 43 154 102 235 251 1 530 71
Cuts at: 1387 1561 1824 1855 2061 2121 2538 3209 3351
Size: 174 263 31 206 60 417 671 142
Fragments arranged by size:
671 530 417 263 251 235 206 174
154 142 102 71 60 43 31 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1047 1170 2730 3351
Size: 1047 123 1560 621
Fragments arranged by size:
1560 1047 621 123
FspI TGC'GCA
Cuts at: 0 957 2413 2500 3351
Size: 957 1456 87 851
Fragments arranged by size:
1456 957 851 87
HaeII r_GCGC'y
Cuts at: 0 150 161 222 398 446 558 672 719
Size: 150 11 61 176 48 112 114 47
Cuts at: 719 1576 1880 2456 3351
Size: 857 304 576 895
Fragments arranged by size:
895 857 576 304 176 150 114 112
61 48 47 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1400 1432 1753 1785 2301 2333 3351
Size: 1400 32 321 32 516 32 1018
Fragments arranged by size:
1400 1018 516 321 32 32 32
HincII GTy'rAC
Cuts at: 0 329 3351
Size: 329 3022
HindIII A'AGCT_T
Cuts at: 0 2380 3351
Size: 2380 971
KasI G'GCGC_C
Cuts at: 0 146 442 554 668 1876 3351
Size: 146 296 112 114 1208 1475
Fragments arranged by size:
1475 1208 296 146 114 112
MscI TGG'CCA
Cuts at: 0 1177 2068 3351
Size: 1177 891 1283
Fragments arranged by size:
1283 1177 891
MslI CAynn'nnrTG
Cuts at: 0 646 2080 2353 2428 2506 2531 2593 3351
Size: 646 1434 273 75 78 25 62 758
Fragments arranged by size:
1434 758 646 273 78 75 62 25
MspA1I CmG'CkG
Cuts at: 0 63 115 144 453 505 567 999 1019
Size: 63 52 29 309 52 62 432 20
Cuts at: 1019 1184 1403 1812 1820 2223 2583 3163 3255
Size: 165 219 409 8 403 360 580 92
Cuts at: 3255 3327 3351
Size: 72 24
Fragments arranged by size:
580 432 409 403 360 309 219 165
92 72 63 62 52 52 29 24
20 8
NaeI GCC'GGC
Cuts at: 0 217 627 3351
Size: 217 410 2724
Fragments arranged by size:
2724 410 217
NarI GG'CG_CC
Cuts at: 0 147 443 555 669 1877 3351
Size: 147 296 112 114 1208 1474
Fragments arranged by size:
1474 1208 296 147 114 112
NcoI C'CATG_G
Cuts at: 0 2426 3351
Size: 2426 925
NdeI CA'TA_TG
Cuts at: 0 849 3351
Size: 849 2502
NgoMIV G'CCGG_C
Cuts at: 0 215 625 3351
Size: 215 410 2726
Fragments arranged by size:
2726 410 215
NruI TCG'CGA
Cuts at: 0 727 2321 3351
Size: 727 1594 1030
Fragments arranged by size:
1594 1030 727
NspI r_CATG'y
Cuts at: 0 2526 2690 3351
Size: 2526 164 661
Fragments arranged by size:
2526 661 164
PasI CC'CwG_GG
Cuts at: 0 201 595 781 1704 2183 2194 3168 3351
Size: 201 394 186 923 479 11 974 183
Fragments arranged by size:
974 923 479 394 201 186 183 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 46 309 759 2183 3351
Size: 46 263 450 1424 1168
Fragments arranged by size:
1424 1168 450 263 46
PfoI T'CCnGG_A
Cuts at: 0 365 1048 2117 2717 2813 2984 3351
Size: 365 683 1069 600 96 171 367
Fragments arranged by size:
1069 683 600 367 365 171 96
PmlI CAC'GTG
Cuts at: 0 1788 3351
Size: 1788 1563
PpuMI rG'GwC_Cy
Cuts at: 0 1824 2061 2121 3351
Size: 1824 237 60 1230
Fragments arranged by size:
1824 1230 237 60
PspOMI G'GGCC_C
Cuts at: 0 197 299 785 1561 3351
Size: 197 102 486 776 1790
Fragments arranged by size:
1790 776 486 197 102
PspXI vC'TCGA_Gb
Cuts at: 0 2242 3351
Size: 2242 1109
PstI C_TGCA'G
Cuts at: 0 1238 2285 3351
Size: 1238 1047 1066
Fragments arranged by size:
1238 1066 1047
PvuII CAG'CTG
Cuts at: 0 999 1184 1812 3351
Size: 999 185 628 1539
Fragments arranged by size:
1539 999 628 185
SacI G_AGCT'C
Cuts at: 0 2624 3351
Size: 2624 727
SacII CC_GC'GG
Cuts at: 0 506 3351
Size: 506 2845
SapI GCTCTTCn'nnn_
Cuts at: 0 600 2936 3351
Size: 600 2336 415
Fragments arranged by size:
2336 600 415
ScaI AGT'ACT
Cuts at: 0 913 3351
Size: 913 2438
SfcI C'TryA_G
Cuts at: 0 1234 2281 2745 3351
Size: 1234 1047 464 606
Fragments arranged by size:
1234 1047 606 464
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 599 3351
Size: 599 2752
SfoI GGC'GCC
Cuts at: 0 148 444 556 670 1878 3351
Size: 148 296 112 114 1208 1473
Fragments arranged by size:
1473 1208 296 148 114 112
SgrAI Cr'CCGG_yG
Cuts at: 0 215 3351
Size: 215 3136
SmlI C'TyrA_G
Cuts at: 0 1964 2242 2563 2933 3351
Size: 1964 278 321 370 418
Fragments arranged by size:
1964 418 370 321 278
SphI G_CATG'C
Cuts at: 0 2526 3351
Size: 2526 825
StuI AGG'CCT
Cuts at: 0 124 709 2572 3351
Size: 124 585 1863 779
Fragments arranged by size:
1863 779 585 124
StyI C'CwwG_G
Cuts at: 0 1565 2057 2342 2426 3351
Size: 1565 492 285 84 925
Fragments arranged by size:
1565 925 492 285 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 301 1880 2771 2910 3351
Size: 301 1579 891 139 441
Fragments arranged by size:
1579 891 441 301 139
TatI w'GTAC_w
Cuts at: 0 820 911 3351
Size: 820 91 2440
Fragments arranged by size:
2440 820 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1099 1131 3351
Size: 1099 32 2220
Fragments arranged by size:
2220 1099 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1572 1701 2268 3351
Size: 1572 129 567 1083
Fragments arranged by size:
1572 1083 567 129
XhoI C'TCGA_G
Cuts at: 0 1964 2242 3351
Size: 1964 278 1109
Fragments arranged by size:
1964 1109 278
XmnI GAAnn'nnTTC
Cuts at: 0 1135 2971 3351
Size: 1135 1836 380
Fragments arranged by size:
1836 1135 380
ZraI GAC'GTC
Cuts at: 0 2267 3351
Size: 2267 1084
Enzymes that do cut:
AarI AatII AcuI AflIII AgeI AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII HindIII KasI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI
NspI PasI PflMI PfoI PmlI PpuMI PspOMI PspXI
PstI PvuII SacI SacII SapI ScaI SfcI SfiI
SfoI SgrAI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AloI AscI AseI
AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmtI
BsaXI BsiWI BsmI BsrDI BsrGI BssHII BstBI BstEII
BstZ17I ClaI DraI DraIII EcoRI EcoRV FseI FspAI
HpaI KpnI MfeI MluI MmeI NheI NotI NsiI
PacI PciI PmeI PpiI PshAI PsiI PsrI PvuI
RsrII SalI SanDI SbfI SexAI SgfI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XmaI