Restriction map for
pF1KE3378
(Linear) (Six-base) MAPSORT of: KIEE3378.seq Check: 1169 from: 1 to: 3390
KIEE3378.orf
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AccI GT'mk_AC
Cuts at: 0 1971 3390
Size: 1971 1419
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 352 1661 2294 2541 2692 2777 2957
Size: 278 74 1309 633 247 151 85 180
Cuts at: 2957 3390
Size: 433
Fragments arranged by size:
1309 633 433 278 247 180 151 85
74
AfeI AGC'GCT
Cuts at: 0 826 3390
Size: 826 2564
AflIII A'CryG_T
Cuts at: 0 1935 3390
Size: 1935 1455
AleI CACnn'nnGTG
Cuts at: 0 2787 3390
Size: 2787 603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 470 3390
Size: 470 2920
AlwNI CAG_nnn'CTG
Cuts at: 0 463 1459 3390
Size: 463 996 1931
Fragments arranged by size:
1931 996 463
ApoI r'AATT_y
Cuts at: 0 98 117 208 239 276 406 1009 1386
Size: 98 19 91 31 37 130 603 377
Cuts at: 1386 1918 3111 3229 3390
Size: 532 1193 118 161
Fragments arranged by size:
1193 603 532 377 161 130 118 98
91 37 31 19
AseI AT'TA_AT
Cuts at: 0 476 2380 3305 3390
Size: 476 1904 925 85
Fragments arranged by size:
1904 925 476 85
AvaI C'yCGr_G
Cuts at: 0 1332 3390
Size: 1332 2058
BamHI G'GATC_C
Cuts at: 0 2640 3390
Size: 2640 750
BanI G'GyrC_C
Cuts at: 0 1230 3390
Size: 1230 2160
BbsI GAAGACnn'nnnn_
Cuts at: 0 642 3390
Size: 642 2748
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 223 257 3390
Size: 223 34 3133
Fragments arranged by size:
3133 223 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1550 2770 3390
Size: 1550 1220 620
Fragments arranged by size:
1550 1220 620
BclI T'GATC_A
Cuts at: 0 3342 3390
Size: 3342 48
BfrBI ATG'CAT
Cuts at: 0 3220 3390
Size: 3220 170
BlpI GC'TnA_GC
Cuts at: 0 149 3390
Size: 149 3241
BmrI ACTGGGnnnn_n'
Cuts at: 0 1317 1580 3390
Size: 1317 263 1810
Fragments arranged by size:
1810 1317 263
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 752 3390
Size: 752 2638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1301 3030 3390
Size: 1301 1729 360
Fragments arranged by size:
1729 1301 360
Bpu10I CC'TnA_GC
Cuts at: 0 143 2985 3390
Size: 143 2842 405
Fragments arranged by size:
2842 405 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1892 2006 2631 3390
Size: 704 1188 114 625 759
Fragments arranged by size:
1188 759 704 625 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 1023 3390
Size: 1023 2367
BsaAI yAC'GTr
Cuts at: 0 849 3390
Size: 849 2541
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 575 605 753 774 783 804 838 868
Size: 575 30 148 21 9 21 34 30
Cuts at: 868 1458 1488 2793 2823 3390
Size: 590 30 1305 30 567
Fragments arranged by size:
1305 590 575 567 148 34 30 30
30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 728 1895 3390
Size: 21 707 1167 1495
Fragments arranged by size:
1495 1167 707 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 1675 2462 2532 2678 3390
Size: 523 1152 787 70 146 712
Fragments arranged by size:
1152 787 712 523 146 70
BsmI GAATG_Cn'
Cuts at: 0 1043 3220 3390
Size: 1043 2177 170
Fragments arranged by size:
2177 1043 170
BspHI T'CATG_A
Cuts at: 0 1067 3390
Size: 1067 2323
BsrBI CCG'CTC
Cuts at: 0 2685 3390
Size: 2685 705
BsrFI r'CCGG_y
Cuts at: 0 80 3390
Size: 80 3310
BstAPI GCAn_nnn'nTGC
Cuts at: 0 463 3390
Size: 463 2927
BstBI TT'CG_AA
Cuts at: 0 243 2208 2229 3390
Size: 243 1965 21 1161
Fragments arranged by size:
1965 1161 243 21
BstEII G'GTnAC_C
Cuts at: 0 2593 3390
Size: 2593 797
BstYI r'GATC_y
Cuts at: 0 180 432 649 1177 2640 3390
Size: 180 252 217 528 1463 750
Fragments arranged by size:
1463 750 528 252 217 180
Bsu36I CC'TnA_GG
Cuts at: 0 112 1598 3390
Size: 112 1486 1792
Fragments arranged by size:
1792 1486 112
BtgI C'CryG_G
Cuts at: 0 2602 3390
Size: 2602 788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2724 3390
Size: 2724 666
BtsI GCAGTG_nn'
Cuts at: 0 1344 1457 3390
Size: 1344 113 1933
Fragments arranged by size:
1933 1344 113
DraI TTT'AAA
Cuts at: 0 1917 3030 3390
Size: 1917 1113 360
Fragments arranged by size:
1917 1113 360
EarI CTCTTCn'nnn_
Cuts at: 0 371 1865 2210 2909 3390
Size: 371 1494 345 699 481
Fragments arranged by size:
1494 699 481 371 345
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 352 1301 1661 2294 2541 2692 2777
Size: 278 74 949 360 633 247 151 85
Cuts at: 2777 2957 3030 3390
Size: 180 73 360
Fragments arranged by size:
949 633 360 360 278 247 180 151
85 74 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 752 773 941 3240 3390
Size: 752 21 168 2299 150
Fragments arranged by size:
2299 752 168 150 21
EcoO109I rG'GnC_Cy
Cuts at: 0 2971 3390
Size: 2971 419
EcoRI G'AATT_C
Cuts at: 0 406 3390
Size: 406 2984
EcoRV GAT'ATC
Cuts at: 0 2265 3390
Size: 2265 1125
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2239 3390
Size: 2239 1151
HaeII r_GCGC'y
Cuts at: 0 828 3390
Size: 828 2562
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 753 785 971 1003 3313 3345 3354 3386
Size: 753 32 186 32 2310 32 9 32
Cuts at: 3386 3390
Size: 4
Fragments arranged by size:
2310 753 186 32 32 32 32 9
4
HincII GTy'rAC
Cuts at: 0 173 516 3390
Size: 173 343 2874
Fragments arranged by size:
2874 343 173
HindIII A'AGCT_T
Cuts at: 0 1755 2939 3390
Size: 1755 1184 451
Fragments arranged by size:
1755 1184 451
HpaI GTT'AAC
Cuts at: 0 516 3390
Size: 516 2874
MfeI C'AATT_G
Cuts at: 0 401 3035 3390
Size: 401 2634 355
Fragments arranged by size:
2634 401 355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1992 3311 3390
Size: 1992 1319 79
Fragments arranged by size:
1992 1319 79
MslI CAynn'nnrTG
Cuts at: 0 1079 1375 2787 3390
Size: 1079 296 1412 603
Fragments arranged by size:
1412 1079 603 296
MspA1I CmG'CkG
Cuts at: 0 463 3390
Size: 463 2927
NaeI GCC'GGC
Cuts at: 0 82 3390
Size: 82 3308
NgoMIV G'CCGG_C
Cuts at: 0 80 3390
Size: 80 3310
NruI TCG'CGA
Cuts at: 0 3374 3390
Size: 3374 16
NsiI A_TGCA'T
Cuts at: 0 3222 3390
Size: 3222 168
NspI r_CATG'y
Cuts at: 0 1939 3220 3390
Size: 1939 1281 170
Fragments arranged by size:
1939 1281 170
PciI A'CATG_T
Cuts at: 0 1935 3390
Size: 1935 1455
PpuMI rG'GwC_Cy
Cuts at: 0 2971 3390
Size: 2971 419
PshAI GACnn'nnGTC
Cuts at: 0 642 3063 3390
Size: 642 2421 327
Fragments arranged by size:
2421 642 327
PstI C_TGCA'G
Cuts at: 0 459 3390
Size: 459 2931
PvuII CAG'CTG
Cuts at: 0 463 3390
Size: 463 2927
SapI GCTCTTCn'nnn_
Cuts at: 0 371 1865 3390
Size: 371 1494 1525
Fragments arranged by size:
1525 1494 371
ScaI AGT'ACT
Cuts at: 0 291 2581 3390
Size: 291 2290 809
Fragments arranged by size:
2290 809 291
SfcI C'TryA_G
Cuts at: 0 455 1522 3390
Size: 455 1067 1868
Fragments arranged by size:
1868 1067 455
SmlI C'TyrA_G
Cuts at: 0 719 1907 2021 2646 3390
Size: 719 1188 114 625 744
Fragments arranged by size:
1188 744 719 625 114
SnaBI TAC'GTA
Cuts at: 0 849 3390
Size: 849 2541
SspI AAT'ATT
Cuts at: 0 2499 3390
Size: 2499 891
StuI AGG'CCT
Cuts at: 0 42 3390
Size: 42 3348
StyI C'CwwG_G
Cuts at: 0 1763 3390
Size: 1763 1627
SwaI ATTT'AAAT
Cuts at: 0 1917 3390
Size: 1917 1473
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 219 3110 3390
Size: 219 2891 280
Fragments arranged by size:
2891 280 219
TatI w'GTAC_w
Cuts at: 0 289 1947 2579 3390
Size: 289 1658 632 811
Fragments arranged by size:
1658 811 632 289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 575 607 683 715 2793 2825 3390
Size: 575 32 76 32 2078 32 565
Fragments arranged by size:
2078 575 565 76 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 881 3390
Size: 881 2509
XmnI GAAnn'nnTTC
Cuts at: 0 1608 2453 3390
Size: 1608 845 937
Fragments arranged by size:
1608 937 845
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AlwNI ApoI
AseI AvaI BamHI BanI BbsI BcgI BciVI BclI
BfrBI BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaXI BseRI BsgI BsmI BspHI BsrBI BsrFI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII HpaI MfeI MmeI MslI
MspA1I NaeI NgoMIV NruI NsiI NspI PciI PpuMI
PshAI PstI PvuII SapI ScaI SfcI SmlI SnaBI
SspI StuI StyI SwaI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AloI
ApaI ApaLI AscI AsiSI AvrII BaeI BanII BbeI
BbvCI BglI BglII Bme1580I BmgBI BmtI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspMI BsrDI BsrGI BssHII BssSI BstXI BstZ17I ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoICRI FseI
FspI FspAI KasI KpnI MluI MscI NarI NcoI
NdeI NheI NotI PacI PasI PflMI PfoI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SpeI SphI SrfI Tth111I XbaI
XhoI XmaI ZraI