Restriction map for
pF1KE3379
(Linear) (Six-base) MAPSORT of: KIEE3379.seq Check: 555 from: 1 to: 3390
KIEE3379.orf
With 182 enzymes: SgfI *
June 16, 2011 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3365 3390
Size: 3365 25
AatII G_ACGT'C
Cuts at: 0 949 3236 3390
Size: 949 2287 154
Fragments arranged by size:
2287 949 154
Acc65I G'GTAC_C
Cuts at: 0 2372 3390
Size: 2372 1018
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 2398 2707 2794 3061 3390
Size: 484 1914 309 87 267 329
Fragments arranged by size:
1914 484 329 309 267 87
AflIII A'CryG_T
Cuts at: 0 1040 2535 3390
Size: 1040 1495 855
Fragments arranged by size:
1495 1040 855
AhdI GACnn_n'nnGTC
Cuts at: 0 506 1921 3213 3390
Size: 506 1415 1292 177
Fragments arranged by size:
1415 1292 506 177
AleI CACnn'nnGTG
Cuts at: 0 496 3390
Size: 496 2894
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3293 3390
Size: 3293 97
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2020 2052 2808 2840 3390
Size: 2020 32 756 32 550
Fragments arranged by size:
2020 756 550 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 883 1401 1668 2418 2964 3390
Size: 883 518 267 750 546 426
Fragments arranged by size:
883 750 546 518 426 267
ApaI G_GGCC'C
Cuts at: 0 86 1420 1968 2157 2896 3267 3390
Size: 86 1334 548 189 739 371 123
Fragments arranged by size:
1334 739 548 371 189 123 86
ApoI r'AATT_y
Cuts at: 0 2459 3390
Size: 2459 931
AvaI C'yCGr_G
Cuts at: 0 920 1646 1960 2320 3390
Size: 920 726 314 360 1070
Fragments arranged by size:
1070 920 726 360 314
BamHI G'GATC_C
Cuts at: 0 2933 3390
Size: 2933 457
BanI G'GyrC_C
Cuts at: 0 777 1048 1551 2372 2509 3091 3390
Size: 777 271 503 821 137 582 299
Fragments arranged by size:
821 777 582 503 299 271 137
BanII G_rGCy'C
Cuts at: 0 47 86 191 488 719 1058 1420 1565
Size: 47 39 105 297 231 339 362 145
Cuts at: 1565 1753 1782 1968 2157 2198 2316 2486 2852
Size: 188 29 186 189 41 118 170 366
Cuts at: 2852 2896 3267 3390
Size: 44 371 123
Fragments arranged by size:
371 366 362 339 297 231 189 188
186 170 145 123 118 105 47 44
41 39 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 306 1335 2792 2840 3390
Size: 306 1029 1457 48 550
Fragments arranged by size:
1457 1029 550 306 48
BbvCI CC'TCA_GC
Cuts at: 0 1193 3390
Size: 1193 2197
BglI GCCn_nnn'nGGC
Cuts at: 0 714 1070 1934 1987 2443 2452 2557 3390
Size: 714 356 864 53 456 9 105 833
Fragments arranged by size:
864 833 714 456 356 105 53 9
BlpI GC'TnA_GC
Cuts at: 0 482 3145 3390
Size: 482 2663 245
Fragments arranged by size:
2663 482 245
Bme1580I G_kGCm'C
Cuts at: 0 86 782 1420 1968 2157 2512 2896 3267
Size: 86 696 638 548 189 355 384 371
Cuts at: 3267 3390
Size: 123
Fragments arranged by size:
696 638 548 384 371 355 189 123
86
BmgBI CAC'GTC
Cuts at: 0 2052 3174 3390
Size: 2052 1122 216
Fragments arranged by size:
2052 1122 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 800 1167 1323 2551 3390
Size: 800 367 156 1228 839
Fragments arranged by size:
1228 839 800 367 156
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 210 603 1584 2335 3027 3209 3314 3390
Size: 210 393 981 751 692 182 105 76
Fragments arranged by size:
981 751 692 393 210 182 105 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 191 352 621 625 735 824 992
Size: 124 67 161 269 4 110 89 168
Cuts at: 992 1129 1153 1894 2272 2440 2899 3390
Size: 137 24 741 378 168 459 491
Fragments arranged by size:
741 491 459 378 269 168 168 161
137 124 110 89 67 24 4
Bpu10I CC'TnA_GC
Cuts at: 0 340 1004 1193 1669 1752 3139 3390
Size: 340 664 189 476 83 1387 251
Fragments arranged by size:
1387 664 476 340 251 189 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2685 3390
Size: 2685 705
BsaI GGTCTCn'nnnn_
Cuts at: 0 262 753 984 1461 2704 3390
Size: 262 491 231 477 1243 686
Fragments arranged by size:
1243 686 491 477 262 231
BsaAI yAC'GTr
Cuts at: 0 1043 1541 1731 2472 3390
Size: 1043 498 190 741 918
Fragments arranged by size:
1043 918 741 498 190
BsaHI Gr'CG_yC
Cuts at: 0 946 1682 1889 3233 3325 3390
Size: 946 736 207 1344 92 65
Fragments arranged by size:
1344 946 736 207 92 65
BsaWI w'CCGG_w
Cuts at: 0 3204 3390
Size: 3204 186
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2020 2050 2468 2498 3390
Size: 2020 30 418 30 892
Fragments arranged by size:
2020 892 418 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 1128 1519 1618 1621 1780 2464 2749
Size: 460 668 391 99 3 159 684 285
Cuts at: 2749 3390
Size: 641
Fragments arranged by size:
684 668 641 460 391 285 159 99
3
BseYI C'CCAG_C
Cuts at: 0 25 326 892 1254 1288 1932 1998 2125
Size: 25 301 566 362 34 644 66 127
Cuts at: 2125 2275 2842 2889 3035 3266 3390
Size: 150 567 47 146 231 124
Fragments arranged by size:
644 567 566 362 301 231 150 146
127 124 66 47 34 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1117 1516 1961 2413 2824 3390
Size: 1117 399 445 452 411 566
Fragments arranged by size:
1117 566 452 445 411 399
BsiEI CG_ry'CG
Cuts at: 0 1228 1439 2493 3390
Size: 1228 211 1054 897
Fragments arranged by size:
1228 1054 897 211
BsiHKAI G_wGCw'C
Cuts at: 0 191 941 1220 1753 1769 2198 2486 2852
Size: 191 750 279 533 16 429 288 366
Cuts at: 2852 3390
Size: 538
Fragments arranged by size:
750 538 533 429 366 288 279 191
16
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3301 3390
Size: 3301 89
Bsp1286I G_dGCh'C
Cuts at: 0 47 86 191 488 719 782 941 1058
Size: 47 39 105 297 231 63 159 117
Cuts at: 1058 1220 1420 1565 1753 1769 1782 1968 2157
Size: 162 200 145 188 16 13 186 189
Cuts at: 2157 2198 2316 2486 2512 2852 2896 3267 3390
Size: 41 118 170 26 340 44 371 123
Fragments arranged by size:
371 340 297 231 200 189 188 186
170 162 159 145 123 118 117 105
63 47 44 41 39 26 16 13
BspEI T'CCGG_A
Cuts at: 0 3204 3390
Size: 3204 186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 896 915 3365 3390
Size: 896 19 2450 25
Fragments arranged by size:
2450 896 25 19
BsrBI CCG'CTC
Cuts at: 0 1028 1137 3390
Size: 1028 109 2253
Fragments arranged by size:
2253 1028 109
BsrDI GCAATG_nn'
Cuts at: 0 1359 2146 3390
Size: 1359 787 1244
Fragments arranged by size:
1359 1244 787
BsrFI r'CCGG_y
Cuts at: 0 845 1008 1220 1319 1548 1554 1588 1799
Size: 845 163 212 99 229 6 34 211
Cuts at: 1799 3390
Size: 1591
Fragments arranged by size:
1591 845 229 212 211 163 99 34
6
BsrGI T'GTAC_A
Cuts at: 0 3152 3390
Size: 3152 238
BssSI C'ACGA_G
Cuts at: 0 1238 1429 2440 2626 3194 3390
Size: 1238 191 1011 186 568 196
Fragments arranged by size:
1238 1011 568 196 191 186
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1401 2361 3390
Size: 1401 960 1029
Fragments arranged by size:
1401 1029 960
BstXI CCAn_nnnn'nTGG
Cuts at: 0 78 3390
Size: 78 3312
BstYI r'GATC_y
Cuts at: 0 1089 1398 2933 3390
Size: 1089 309 1535 457
Fragments arranged by size:
1535 1089 457 309
Bsu36I CC'TnA_GG
Cuts at: 0 668 3390
Size: 668 2722
BtgI C'CryG_G
Cuts at: 0 71 711 768 822 1709 2095 3390
Size: 71 640 57 54 887 386 1295
Fragments arranged by size:
1295 887 640 386 71 57 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 134 2080 3331 3390
Size: 134 1946 1251 59
Fragments arranged by size:
1946 1251 134 59
BtsI GCAGTG_nn'
Cuts at: 0 556 1030 1301 1853 2398 2430 3390
Size: 556 474 271 552 545 32 960
Fragments arranged by size:
960 556 552 545 474 271 32
DraIII CAC_nnn'GTG
Cuts at: 0 64 3390
Size: 64 3326
DrdI GACnn_nn'nnGTC
Cuts at: 0 310 3007 3390
Size: 310 2697 383
Fragments arranged by size:
2697 383 310
EaeI y'GGCC_r
Cuts at: 0 591 847 1145 1225 1317 1436 1586 1801
Size: 591 256 298 80 92 119 150 215
Cuts at: 1801 1979 2069 2490 3390
Size: 178 90 421 900
Fragments arranged by size:
900 591 421 298 256 215 178 150
119 92 90 80
EagI C'GGCC_G
Cuts at: 0 1225 1436 2490 3390
Size: 1225 211 1054 900
Fragments arranged by size:
1225 1054 900 211
EarI CTCTTCn'nnn_
Cuts at: 0 173 260 1411 1459 2224 3390
Size: 173 87 1151 48 765 1166
Fragments arranged by size:
1166 1151 765 173 87 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1573 3390
Size: 1573 1817
EcoICRI GAG'CTC
Cuts at: 0 189 1751 2196 2484 2850 3390
Size: 189 1562 445 288 366 540
Fragments arranged by size:
1562 540 445 366 288 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 191 352 484 621 625 735 824
Size: 124 67 161 132 137 4 110 89
Cuts at: 824 992 1129 1153 1894 2272 2398 2440 2707
Size: 168 137 24 741 378 126 42 267
Cuts at: 2707 2794 2899 3061 3390
Size: 87 105 162 329
Fragments arranged by size:
741 378 329 267 168 162 161 137
137 132 126 124 110 105 89 87
67 42 24 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 343 1081 1192 1672 2026 2900 3390
Size: 343 738 111 480 354 874 490
Fragments arranged by size:
874 738 490 480 354 343 111
EcoO109I rG'GnC_Cy
Cuts at: 0 29 82 83 336 673 1866 1964 1965
Size: 29 53 1 253 337 1193 98 1
Cuts at: 1965 2057 2153 2154 2234 2865 2893 2905 3111
Size: 92 96 1 80 631 28 12 206
Cuts at: 3111 3263 3390
Size: 152 127
Fragments arranged by size:
1193 631 337 253 206 152 127 98
96 92 80 53 29 28 12 1
1 1
FseI GG_CCGG'CC
Cuts at: 0 1592 3390
Size: 1592 1798
HaeII r_GCGC'y
Cuts at: 0 2683 3390
Size: 2683 707
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 492 524 803 835 1907 1939 3199 3231
Size: 492 32 279 32 1072 32 1260 32
Cuts at: 3231 3390
Size: 159
Fragments arranged by size:
1260 1072 492 279 159 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 988 2797 3147 3390
Size: 988 1809 350 243
Fragments arranged by size:
1809 988 350 243
KpnI G_GTAC'C
Cuts at: 0 2376 3390
Size: 2376 1014
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 2109 3390
Size: 1007 1102 1281
Fragments arranged by size:
1281 1102 1007
MscI TGG'CCA
Cuts at: 0 1147 1981 2071 3390
Size: 1147 834 90 1319
Fragments arranged by size:
1319 1147 834 90
MslI CAynn'nnrTG
Cuts at: 0 149 496 1078 3390
Size: 149 347 582 2312
Fragments arranged by size:
2312 582 347 149
MspA1I CmG'CkG
Cuts at: 0 330 481 970 1173 2097 2303 2415 2639
Size: 330 151 489 203 924 206 112 224
Cuts at: 2639 2842 3039 3390
Size: 203 197 351
Fragments arranged by size:
924 489 351 330 224 206 203 203
197 151 112
NaeI GCC'GGC
Cuts at: 0 1321 1590 3390
Size: 1321 269 1800
Fragments arranged by size:
1800 1321 269
NcoI C'CATG_G
Cuts at: 0 71 711 768 1709 3390
Size: 71 640 57 941 1681
Fragments arranged by size:
1681 941 640 71 57
NgoMIV G'CCGG_C
Cuts at: 0 1319 1588 3390
Size: 1319 269 1802
Fragments arranged by size:
1802 1319 269
NotI GC'GGCC_GC
Cuts at: 0 1225 3390
Size: 1225 2165
NspI r_CATG'y
Cuts at: 0 900 2539 3390
Size: 900 1639 851
Fragments arranged by size:
1639 900 851
PasI CC'CwG_GG
Cuts at: 0 1413 3390
Size: 1413 1977
PciI A'CATG_T
Cuts at: 0 2535 3390
Size: 2535 855
PfoI T'CCnGG_A
Cuts at: 0 317 1793 3347 3390
Size: 317 1476 1554 43
Fragments arranged by size:
1554 1476 317 43
PmlI CAC'GTG
Cuts at: 0 1541 2472 3390
Size: 1541 931 918
Fragments arranged by size:
1541 931 918
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2020 2052 2725 2757 3390
Size: 2020 32 673 32 633
Fragments arranged by size:
2020 673 633 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 29 673 2057 2865 3390
Size: 29 644 1384 808 525
Fragments arranged by size:
1384 808 644 525 29
PspOMI G'GGCC_C
Cuts at: 0 82 1416 1964 2153 2892 3263 3390
Size: 82 1334 548 189 739 371 127
Fragments arranged by size:
1334 739 548 371 189 127 82
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3232 3264 3390
Size: 3232 32 126
Fragments arranged by size:
3232 126 32
PstI C_TGCA'G
Cuts at: 0 1037 1397 2390 2960 3390
Size: 1037 360 993 570 430
Fragments arranged by size:
1037 993 570 430 360
PvuII CAG'CTG
Cuts at: 0 330 481 970 1173 2415 2639 2842 3039
Size: 330 151 489 203 1242 224 203 197
Cuts at: 3039 3390
Size: 351
Fragments arranged by size:
1242 489 351 330 224 203 203 197
151
RsrII CG'GwC_CG
Cuts at: 0 1546 3390
Size: 1546 1844
SacI G_AGCT'C
Cuts at: 0 191 1753 2198 2486 2852 3390
Size: 191 1562 445 288 366 538
Fragments arranged by size:
1562 538 445 366 288 191
SacII CC_GC'GG
Cuts at: 0 2098 3390
Size: 2098 1292
SanDI GG'GwC_CC
Cuts at: 0 673 3390
Size: 673 2717
SapI GCTCTTCn'nnn_
Cuts at: 0 1411 3390
Size: 1411 1979
SbfI CC_TGCA'GG
Cuts at: 0 1397 3390
Size: 1397 1993
SfcI C'TryA_G
Cuts at: 0 1033 1393 2023 2386 2956 3390
Size: 1033 360 630 363 570 434
Fragments arranged by size:
1033 630 570 434 363 360
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1934 1987 3390
Size: 1934 53 1403
Fragments arranged by size:
1934 1403 53
SgrAI Cr'CCGG_yG
Cuts at: 0 1220 1554 3390
Size: 1220 334 1836
Fragments arranged by size:
1836 1220 334
SmaI CCC'GGG
Cuts at: 0 1648 1962 3390
Size: 1648 314 1428
Fragments arranged by size:
1648 1428 314
SmlI C'TyrA_G
Cuts at: 0 2664 3390
Size: 2664 726
SphI G_CATG'C
Cuts at: 0 900 3390
Size: 900 2490
StuI AGG'CCT
Cuts at: 0 1187 2551 3390
Size: 1187 1364 839
Fragments arranged by size:
1364 1187 839
StyI C'CwwG_G
Cuts at: 0 48 71 711 768 1179 1211 1709 2221
Size: 48 23 640 57 411 32 498 512
Cuts at: 2221 2552 3047 3390
Size: 331 495 343
Fragments arranged by size:
640 512 498 495 411 343 331 57
48 32 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 970 2105 3079 3390
Size: 970 1135 974 311
Fragments arranged by size:
1135 974 970 311
TatI w'GTAC_w
Cuts at: 0 2538 3152 3239 3390
Size: 2538 614 87 151
Fragments arranged by size:
2538 614 151 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 931 963 2786 2818 3390
Size: 931 32 1823 32 572
Fragments arranged by size:
1823 931 572 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1339 3390
Size: 1339 2051
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 75 3390
Size: 75 3315
XmaI C'CCGG_G
Cuts at: 0 1646 1960 3390
Size: 1646 314 1430
Fragments arranged by size:
1646 1430 314
ZraI GAC'GTC
Cuts at: 0 947 3234 3390
Size: 947 2287 156
Fragments arranged by size:
2287 947 156
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI
HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NotI NspI PasI PciI PfoI
PmlI PpiI PpuMI PspOMI PsrI PstI PvuII RsrII
SacI SacII SanDI SapI SbfI SfcI SfiI SgrAI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI
BglII BmtI BsaBI BsiWI BsmI BspHI BssHII BstBI
BstEII BstZ17I ClaI CspCI DraI EcoRI EcoRV FalI
FspI FspAI HincII HpaI KasI MfeI MluI NarI
NdeI NheI NruI NsiI PacI PflMI PmeI PshAI
PsiI PspXI PvuI SalI ScaI SexAI SfoI SgfI
SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmnI