Restriction map for
pF1KE3386
(Linear) (Six-base) MAPSORT of: KIEE3386.seq Check: 405 from: 1 to: 3621
KIEE3386.orf
With 182 enzymes: SgfI *
June 25, 2015 11:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2359 3621
Size: 2359 1262
AclI AA'CG_TT
Cuts at: 0 1761 3621
Size: 1761 1860
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 509 1292 1418 1618 2197 3070 3621
Size: 107 402 783 126 200 579 873 551
Fragments arranged by size:
873 783 579 551 402 200 126 107
AflIII A'CryG_T
Cuts at: 0 1303 2335 2560 3621
Size: 1303 1032 225 1061
Fragments arranged by size:
1303 1061 1032 225
AhdI GACnn_n'nnGTC
Cuts at: 0 1002 2415 3621
Size: 1002 1413 1206
Fragments arranged by size:
1413 1206 1002
AleI CACnn'nnGTG
Cuts at: 0 1011 2765 3621
Size: 1011 1754 856
Fragments arranged by size:
1754 1011 856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 917 2247 3621
Size: 917 1330 1374
Fragments arranged by size:
1374 1330 917
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1796 1828 3621
Size: 1796 32 1793
Fragments arranged by size:
1796 1793 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3183 3343 3621
Size: 3183 160 278
Fragments arranged by size:
3183 278 160
ApaI G_GGCC'C
Cuts at: 0 3162 3588 3621
Size: 3162 426 33
Fragments arranged by size:
3162 426 33
ApaLI G'TGCA_C
Cuts at: 0 2758 3621
Size: 2758 863
ApoI r'AATT_y
Cuts at: 0 40 111 3621
Size: 40 71 3510
Fragments arranged by size:
3510 71 40
AseI AT'TA_AT
Cuts at: 0 1856 3621
Size: 1856 1765
BamHI G'GATC_C
Cuts at: 0 1126 3621
Size: 1126 2495
BanII G_rGCy'C
Cuts at: 0 3162 3404 3466 3588 3621
Size: 3162 242 62 122 33
Fragments arranged by size:
3162 242 122 62 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 515 805 2037 3621
Size: 515 290 1232 1584
Fragments arranged by size:
1584 1232 515 290
BciVI GTATCCnnnnn_n'
Cuts at: 0 1024 1380 3459 3621
Size: 1024 356 2079 162
Fragments arranged by size:
2079 1024 356 162
BclI T'GATC_A
Cuts at: 0 230 3621
Size: 230 3391
BfrBI ATG'CAT
Cuts at: 0 1488 2998 3621
Size: 1488 1510 623
Fragments arranged by size:
1510 1488 623
BglI GCCn_nnn'nGGC
Cuts at: 0 3074 3621
Size: 3074 547
BglII A'GATC_T
Cuts at: 0 555 3621
Size: 555 3066
BlpI GC'TnA_GC
Cuts at: 0 1519 3621
Size: 1519 2102
Bme1580I G_kGCm'C
Cuts at: 0 1147 2644 2762 3162 3424 3588 3621
Size: 1147 1497 118 400 262 164 33
Fragments arranged by size:
1497 1147 400 262 164 118 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 1360 1925 2629 2761 3399 3621
Size: 1360 565 704 132 638 222
Fragments arranged by size:
1360 704 638 565 222 132
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 574 1806 2176 3621
Size: 574 1232 370 1445
Fragments arranged by size:
1445 1232 574 370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 985 3621
Size: 96 889 2636
Fragments arranged by size:
2636 889 96
Bpu10I CC'TnA_GC
Cuts at: 0 938 3145 3621
Size: 938 2207 476
Fragments arranged by size:
2207 938 476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1306 1454 3304 3621
Size: 1306 148 1850 317
Fragments arranged by size:
1850 1306 317 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 1040 3038 3621
Size: 1040 1998 583
Fragments arranged by size:
1998 1040 583
BsaAI yAC'GTr
Cuts at: 0 1142 3621
Size: 1142 2479
BsaBI GATnn'nnATC
Cuts at: 0 2354 3621
Size: 2354 1267
BsaHI Gr'CG_yC
Cuts at: 0 2826 3621
Size: 2826 795
BsaWI w'CCGG_w
Cuts at: 0 1049 3621
Size: 1049 2572
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 695 725 1649 1679 3391 3421 3621
Size: 695 30 924 30 1712 30 200
Fragments arranged by size:
1712 924 695 200 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2643 3130 3229 3249 3621
Size: 2643 487 99 20 372
Fragments arranged by size:
2643 487 372 99 20
BseYI C'CCAG_C
Cuts at: 0 1631 2584 3621
Size: 1631 953 1037
Fragments arranged by size:
1631 1037 953
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 1043 1122 1448 2769 3621
Size: 116 927 79 326 1321 852
Fragments arranged by size:
1321 927 852 326 116 79
BsiHKAI G_wGCw'C
Cuts at: 0 2508 2698 2762 3621
Size: 2508 190 64 859
Fragments arranged by size:
2508 859 190 64
BsmI GAATG_Cn'
Cuts at: 0 1225 2781 3621
Size: 1225 1556 840
Fragments arranged by size:
1556 1225 840
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2164 3621
Size: 2164 1457
Bsp1286I G_dGCh'C
Cuts at: 0 1147 2508 2644 2698 2762 3162 3404 3424
Size: 1147 1361 136 54 64 400 242 20
Cuts at: 3424 3466 3588 3621
Size: 42 122 33
Fragments arranged by size:
1361 1147 400 242 136 122 64 54
42 33 20
BspHI T'CATG_A
Cuts at: 0 1554 3621
Size: 1554 2067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2359 2818 3621
Size: 2359 459 803
Fragments arranged by size:
2359 803 459
BsrDI GCAATG_nn'
Cuts at: 0 758 1215 3621
Size: 758 457 2406
Fragments arranged by size:
2406 758 457
BsrFI r'CCGG_y
Cuts at: 0 2690 3621
Size: 2690 931
BssSI C'ACGA_G
Cuts at: 0 1799 3621
Size: 1799 1822
BstEII G'GTnAC_C
Cuts at: 0 766 2053 3621
Size: 766 1287 1568
Fragments arranged by size:
1568 1287 766
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1373 1891 2716 3389 3412 3621
Size: 1373 518 825 673 23 209
Fragments arranged by size:
1373 825 673 518 209 23
BstYI r'GATC_y
Cuts at: 0 555 1126 2226 3621
Size: 555 571 1100 1395
Fragments arranged by size:
1395 1100 571 555
Bsu36I CC'TnA_GG
Cuts at: 0 2878 3621
Size: 2878 743
BtgI C'CryG_G
Cuts at: 0 153 3078 3621
Size: 153 2925 543
Fragments arranged by size:
2925 543 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3045 3621
Size: 3045 576
BtsI GCAGTG_nn'
Cuts at: 0 2078 3621
Size: 2078 1543
DraI TTT'AAA
Cuts at: 0 470 1751 3621
Size: 470 1281 1870
Fragments arranged by size:
1870 1281 470
DraIII CAC_nnn'GTG
Cuts at: 0 877 986 3621
Size: 877 109 2635
Fragments arranged by size:
2635 877 109
DrdI GACnn_nn'nnGTC
Cuts at: 0 2430 3621
Size: 2430 1191
EaeI y'GGCC_r
Cuts at: 0 1695 1823 1893 2003 3075 3354 3621
Size: 1695 128 70 110 1072 279 267
Fragments arranged by size:
1695 1072 279 267 128 110 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 107 509 985 1292 1418 1618 2197
Size: 96 11 402 476 307 126 200 579
Cuts at: 2197 3070 3621
Size: 873 551
Fragments arranged by size:
873 579 551 476 402 307 200 126
96 11
EcoO109I rG'GnC_Cy
Cuts at: 0 127 1252 3158 3584 3585 3621
Size: 127 1125 1906 426 1 36
Fragments arranged by size:
1906 1125 426 127 36 1
EcoRI G'AATT_C
Cuts at: 0 111 3621
Size: 111 3510
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1596 3621
Size: 1596 2025
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 695 727 988 1020 2401 2433 2712 2744
Size: 695 32 261 32 1381 32 279 32
Cuts at: 2744 3253 3285 3448 3480 3621
Size: 509 32 163 32 141
Fragments arranged by size:
1381 695 509 279 261 163 141 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3334 3621
Size: 3334 287
HindIII A'AGCT_T
Cuts at: 0 3489 3621
Size: 3489 132
MfeI C'AATT_G
Cuts at: 0 202 3621
Size: 202 3419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 754 3621
Size: 566 188 2867
Fragments arranged by size:
2867 566 188
MscI TGG'CCA
Cuts at: 0 1825 2005 3077 3356 3621
Size: 1825 180 1072 279 265
Fragments arranged by size:
1825 1072 279 265 180
MslI CAynn'nnrTG
Cuts at: 0 248 1011 1232 1493 2467 2565 2765 2960
Size: 248 763 221 261 974 98 200 195
Cuts at: 2960 3103 3621
Size: 143 518
Fragments arranged by size:
974 763 518 261 248 221 200 195
143 98
MspA1I CmG'CkG
Cuts at: 0 610 2361 2765 2783 3621
Size: 610 1751 404 18 838
Fragments arranged by size:
1751 838 610 404 18
NcoI C'CATG_G
Cuts at: 0 153 3621
Size: 153 3468
NdeI CA'TA_TG
Cuts at: 0 3497 3621
Size: 3497 124
NsiI A_TGCA'T
Cuts at: 0 1490 3000 3621
Size: 1490 1510 621
Fragments arranged by size:
1510 1490 621
NspI r_CATG'y
Cuts at: 0 1020 1307 1488 2175 2466 2526 2564 2817
Size: 1020 287 181 687 291 60 38 253
Cuts at: 2817 2973 3002 3621
Size: 156 29 619
Fragments arranged by size:
1020 687 619 291 287 253 181 156
60 38 29
PciI A'CATG_T
Cuts at: 0 1303 2560 3621
Size: 1303 1257 1061
Fragments arranged by size:
1303 1257 1061
PflMI CCAn_nnn'nTGG
Cuts at: 0 1530 3343 3480 3621
Size: 1530 1813 137 141
Fragments arranged by size:
1813 1530 141 137
PmlI CAC'GTG
Cuts at: 0 1142 3621
Size: 1142 2479
PpuMI rG'GwC_Cy
Cuts at: 0 127 1252 3621
Size: 127 1125 2369
Fragments arranged by size:
2369 1125 127
PshAI GACnn'nnGTC
Cuts at: 0 2829 3621
Size: 2829 792
PsiI TTA'TAA
Cuts at: 0 248 3301 3621
Size: 248 3053 320
Fragments arranged by size:
3053 320 248
PspOMI G'GGCC_C
Cuts at: 0 3158 3584 3621
Size: 3158 426 37
Fragments arranged by size:
3158 426 37
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1142 1174 1299 1331 3621
Size: 1142 32 125 32 2290
Fragments arranged by size:
2290 1142 125 32 32
PstI C_TGCA'G
Cuts at: 0 976 3621
Size: 976 2645
PvuII CAG'CTG
Cuts at: 0 610 2361 2765 2783 3621
Size: 610 1751 404 18 838
Fragments arranged by size:
1751 838 610 404 18
ScaI AGT'ACT
Cuts at: 0 1057 3621
Size: 1057 2564
SfcI C'TryA_G
Cuts at: 0 972 1148 2066 3168 3621
Size: 972 176 918 1102 453
Fragments arranged by size:
1102 972 918 453 176
SmlI C'TyrA_G
Cuts at: 0 1285 1469 3319 3621
Size: 1285 184 1850 302
Fragments arranged by size:
1850 1285 302 184
SphI G_CATG'C
Cuts at: 0 2175 3002 3621
Size: 2175 827 619
Fragments arranged by size:
2175 827 619
StuI AGG'CCT
Cuts at: 0 1889 3246 3621
Size: 1889 1357 375
Fragments arranged by size:
1889 1357 375
StyI C'CwwG_G
Cuts at: 0 153 911 1884 1890 2163 2709 3286 3621
Size: 153 758 973 6 273 546 577 335
Fragments arranged by size:
973 758 577 546 335 273 153 6
SwaI ATTT'AAAT
Cuts at: 0 1751 3621
Size: 1751 1870
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 258 1097 2072 2617 3621
Size: 258 839 975 545 1004
Fragments arranged by size:
1004 975 839 545 258
TatI w'GTAC_w
Cuts at: 0 653 1055 1099 1967 3621
Size: 653 402 44 868 1654
Fragments arranged by size:
1654 868 653 402 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1044 1076 1649 1681 2074 2106 2884 2916
Size: 1044 32 573 32 393 32 778 32
Cuts at: 2916 3621
Size: 705
Fragments arranged by size:
1044 778 705 573 393 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3364 3621
Size: 3364 257
XmnI GAAnn'nnTTC
Cuts at: 0 444 672 3621
Size: 444 228 2949
Fragments arranged by size:
2949 444 228
Enzymes that do cut:
AarI AclI AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI BamHI BanII BbsI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI
NspI PciI PflMI PmlI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuII ScaI SfcI SmlI SphI StuI
StyI SwaI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AscI AsiSI
AvaI AvrII BaeI BanI BbeI BbvCI BcgI BmgBI
BmtI BsiEI BsiWI BspEI BsrBI BsrGI BssHII BstAPI
BstBI BstZ17I ClaI CspCI EagI EarI EciI EcoICRI
EcoNI EcoRV FseI FspI FspAI HaeII HpaI KasI
KpnI MluI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PfoI PmeI PpiI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
Tth111I XbaI XhoI XmaI ZraI