Restriction map for
pF1KE3393
(Linear) (Six-base) MAPSORT of: KIEE3393.seq Check: 9847 from: 1 to: 3564
KIEE3393.orf
With 182 enzymes: SgfI *
September 21, 2017 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2985 3564
Size: 2985 579
AccI GT'mk_AC
Cuts at: 0 265 695 3564
Size: 265 430 2869
Fragments arranged by size:
2869 430 265
Acc65I G'GTAC_C
Cuts at: 0 3166 3564
Size: 3166 398
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 727 872 947 1023 1703 2293 2822
Size: 473 254 145 75 76 680 590 529
Cuts at: 2822 3119 3428 3564
Size: 297 309 136
Fragments arranged by size:
680 590 529 473 309 297 254 145
136 76 75
AlwNI CAG_nnn'CTG
Cuts at: 0 3505 3564
Size: 3505 59
ApoI r'AATT_y
Cuts at: 0 658 1100 1131 1431 1769 2124 2175 2694
Size: 658 442 31 300 338 355 51 519
Cuts at: 2694 3564
Size: 870
Fragments arranged by size:
870 658 519 442 355 338 300 51
31
AseI AT'TA_AT
Cuts at: 0 3244 3564
Size: 3244 320
AvaI C'yCGr_G
Cuts at: 0 1421 3564
Size: 1421 2143
BanI G'GyrC_C
Cuts at: 0 64 903 3166 3564
Size: 64 839 2263 398
Fragments arranged by size:
2263 839 398 64
BanII G_rGCy'C
Cuts at: 0 725 3564
Size: 725 2839
BbsI GAAGACnn'nnnn_
Cuts at: 0 421 500 977 1475 1709 1765 2388 2483
Size: 421 79 477 498 234 56 623 95
Cuts at: 2483 3564
Size: 1081
Fragments arranged by size:
1081 623 498 477 421 234 95 79
56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 31 65 3564
Size: 31 34 3499
Fragments arranged by size:
3499 34 31
BciVI GTATCCnnnnn_n'
Cuts at: 0 581 3564
Size: 581 2983
BfrBI ATG'CAT
Cuts at: 0 2762 3564
Size: 2762 802
BglII A'GATC_T
Cuts at: 0 433 1017 2400 3564
Size: 433 584 1383 1164
Fragments arranged by size:
1383 1164 584 433
BmrI ACTGGGnnnn_n'
Cuts at: 0 1564 3564
Size: 1564 2000
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 455 1436 2973 3366 3564
Size: 455 981 1537 393 198
Fragments arranged by size:
1537 981 455 393 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 931 3564
Size: 383 548 2633
Fragments arranged by size:
2633 548 383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 2630 3092 3564
Size: 835 1795 462 472
Fragments arranged by size:
1795 835 472 462
BsaI GGTCTCn'nnnn_
Cuts at: 0 3121 3564
Size: 3121 443
BsaBI GATnn'nnATC
Cuts at: 0 1022 3564
Size: 1022 2542
BsaWI w'CCGG_w
Cuts at: 0 711 3564
Size: 711 2853
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 179 209 3564
Size: 179 30 3355
Fragments arranged by size:
3355 179 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1204 1990 3444 3564
Size: 1204 786 1454 120
Fragments arranged by size:
1454 1204 786 120
BseYI C'CCAG_C
Cuts at: 0 592 846 3564
Size: 592 254 2718
Fragments arranged by size:
2718 592 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2204 3564
Size: 2204 1360
BsiHKAI G_wGCw'C
Cuts at: 0 725 3564
Size: 725 2839
BsmI GAATG_Cn'
Cuts at: 0 1118 2735 3562 3564
Size: 1118 1617 827 2
Fragments arranged by size:
1617 1118 827 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 297 511 3564
Size: 297 214 3053
Fragments arranged by size:
3053 297 214
Bsp1286I G_dGCh'C
Cuts at: 0 725 3564
Size: 725 2839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2985 3564
Size: 2985 579
BsrDI GCAATG_nn'
Cuts at: 0 2849 3564
Size: 2849 715
BsrGI T'GTAC_A
Cuts at: 0 3183 3564
Size: 3183 381
BstXI CCAn_nnnn'nTGG
Cuts at: 0 147 3564
Size: 147 3417
BstYI r'GATC_y
Cuts at: 0 217 433 1017 2400 2671 3564
Size: 217 216 584 1383 271 893
Fragments arranged by size:
1383 893 584 271 217 216
BstZ17I GTA'TAC
Cuts at: 0 266 3564
Size: 266 3298
Bsu36I CC'TnA_GG
Cuts at: 0 908 1340 3564
Size: 908 432 2224
Fragments arranged by size:
2224 908 432
BtgI C'CryG_G
Cuts at: 0 269 3564
Size: 269 3295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2573 3564
Size: 2573 991
BtsI GCAGTG_nn'
Cuts at: 0 483 3564
Size: 483 3081
ClaI AT'CG_AT
Cuts at: 0 1352 3564
Size: 1352 2212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 986 1021 3564
Size: 986 35 2543
Fragments arranged by size:
2543 986 35
DraI TTT'AAA
Cuts at: 0 2012 3564
Size: 2012 1552
EaeI y'GGCC_r
Cuts at: 0 2436 3564
Size: 2436 1128
EarI CTCTTCn'nnn_
Cuts at: 0 446 476 860 893 923 1033 1042 1280
Size: 446 30 384 33 30 110 9 238
Cuts at: 1280 1324 1373 1451 1625 1631 3211 3564
Size: 44 49 78 174 6 1580 353
Fragments arranged by size:
1580 446 384 353 238 174 110 78
49 44 33 30 30 9 6
EcoICRI GAG'CTC
Cuts at: 0 723 3564
Size: 723 2841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 473 727 872 931 947 1023 1703
Size: 383 90 254 145 59 16 76 680
Cuts at: 1703 2293 2822 3119 3428 3564
Size: 590 529 297 309 136
Fragments arranged by size:
680 590 529 383 309 297 254 145
136 90 76 59 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 127 3564
Size: 127 3437
EcoO109I rG'GnC_Cy
Cuts at: 0 172 3564
Size: 172 3392
EcoRI G'AATT_C
Cuts at: 0 1131 3564
Size: 1131 2433
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 452 3564
Size: 452 3112
HaeII r_GCGC'y
Cuts at: 0 504 3564
Size: 504 3060
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1606 1638 1832 1864 2385 2417 3115 3147
Size: 1606 32 194 32 521 32 698 32
Cuts at: 3147 3564
Size: 417
Fragments arranged by size:
1606 698 521 417 194 32 32 32
32
HincII GTy'rAC
Cuts at: 0 248 3564
Size: 248 3316
KpnI G_GTAC'C
Cuts at: 0 3170 3564
Size: 3170 394
MfeI C'AATT_G
Cuts at: 0 145 3564
Size: 145 3419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 2265 2790 3021 3564
Size: 165 2100 525 231 543
Fragments arranged by size:
2100 543 525 231 165
MscI TGG'CCA
Cuts at: 0 2438 3564
Size: 2438 1126
MslI CAynn'nnrTG
Cuts at: 0 613 1058 1235 1835 3508 3564
Size: 613 445 177 600 1673 56
Fragments arranged by size:
1673 613 600 445 177 56
MspA1I CmG'CkG
Cuts at: 0 596 850 1138 2800 3564
Size: 596 254 288 1662 764
Fragments arranged by size:
1662 764 596 288 254
NsiI A_TGCA'T
Cuts at: 0 2764 3564
Size: 2764 800
NspI r_CATG'y
Cuts at: 0 2037 3564
Size: 2037 1527
PflMI CCAn_nnn'nTGG
Cuts at: 0 3229 3564
Size: 3229 335
PpuMI rG'GwC_Cy
Cuts at: 0 172 3564
Size: 172 3392
PsiI TTA'TAA
Cuts at: 0 959 2419 3564
Size: 959 1460 1145
Fragments arranged by size:
1460 1145 959
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2288 2320 3564
Size: 2288 32 1244
Fragments arranged by size:
2288 1244 32
PstI C_TGCA'G
Cuts at: 0 1530 3291 3564
Size: 1530 1761 273
Fragments arranged by size:
1761 1530 273
PvuII CAG'CTG
Cuts at: 0 596 850 1138 2800 3564
Size: 596 254 288 1662 764
Fragments arranged by size:
1662 764 596 288 254
SacI G_AGCT'C
Cuts at: 0 725 3564
Size: 725 2839
ScaI AGT'ACT
Cuts at: 0 1195 3564
Size: 1195 2369
SfcI C'TryA_G
Cuts at: 0 668 1526 2522 2990 3171 3287 3370 3551
Size: 668 858 996 468 181 116 83 181
Cuts at: 3551 3564
Size: 13
Fragments arranged by size:
996 858 668 468 181 181 116 83
13
SmlI C'TyrA_G
Cuts at: 0 814 1421 2645 3107 3564
Size: 814 607 1224 462 457
Fragments arranged by size:
1224 814 607 462 457
SpeI A'CTAG_T
Cuts at: 0 2776 3564
Size: 2776 788
SspI AAT'ATT
Cuts at: 0 1074 1212 2817 3388 3564
Size: 1074 138 1605 571 176
Fragments arranged by size:
1605 1074 571 176 138
StyI C'CwwG_G
Cuts at: 0 1113 2867 3564
Size: 1113 1754 697
Fragments arranged by size:
1754 1113 697
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1854 3564
Size: 1854 1710
TatI w'GTAC_w
Cuts at: 0 1193 2304 3183 3337 3543 3564
Size: 1193 1111 879 154 206 21
Fragments arranged by size:
1193 1111 879 206 154 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1718 1750 3564
Size: 1718 32 1814
Fragments arranged by size:
1814 1718 32
XhoI C'TCGA_G
Cuts at: 0 1421 3564
Size: 1421 2143
Enzymes that do cut:
AarI AccI Acc65I AcuI AlwNI ApoI AseI AvaI
BanI BanII BbsI BcgI BciVI BfrBI BglII BmrI
BplI BpmI BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BsrGI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII KpnI MfeI
MmeI MscI MslI MspA1I NsiI NspI PflMI PpuMI
PsiI PsrI PstI PvuII SacI ScaI SfcI SmlI
SpeI SspI StyI TaqII TatI TstI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BbeI BbvCI BclI BglI BlpI Bme1580I BmgBI
BmtI Bpu10I BsaAI BsaHI BsiEI BsiWI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII DraIII
DrdI EagI EciI EcoRV FseI FspI FspAI HindIII
HpaI KasI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI StuI SwaI Tth111I
XbaI XcmI XmaI XmnI ZraI