Restriction map for
pF1KE3451
(Linear) (Six-base) MAPSORT of: KIEE3451.seq Check: 4047 from: 1 to: 3963
KIEE3451.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 0 1888 3963
Size: 1888 2075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1211 3963
Size: 802 409 2752
Fragments arranged by size:
2752 802 409
AfeI AGC'GCT
Cuts at: 0 2865 3963
Size: 2865 1098
AflII C'TTAA_G
Cuts at: 0 22 3963
Size: 22 3941
AflIII A'CryG_T
Cuts at: 0 1538 3963
Size: 1538 2425
AhdI GACnn_n'nnGTC
Cuts at: 0 666 3963
Size: 666 3297
AleI CACnn'nnGTG
Cuts at: 0 1091 3963
Size: 1091 2872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 409 3371 3511 3963
Size: 409 2962 140 452
Fragments arranged by size:
2962 452 409 140
AlwNI CAG_nnn'CTG
Cuts at: 0 1039 1488 1549 1675 1806 1845 3264 3759
Size: 1039 449 61 126 131 39 1419 495
Cuts at: 3759 3963
Size: 204
Fragments arranged by size:
1419 1039 495 449 204 131 126 61
39
ApaI G_GGCC'C
Cuts at: 0 1150 2925 3003 3652 3843 3963
Size: 1150 1775 78 649 191 120
Fragments arranged by size:
1775 1150 649 191 120 78
ApaLI G'TGCA_C
Cuts at: 0 2791 3963
Size: 2791 1172
AvaI C'yCGr_G
Cuts at: 0 1123 1174 1711 1732 1784 1932 2691 2924
Size: 1123 51 537 21 52 148 759 233
Cuts at: 2924 3005 3802 3818 3836 3865 3963
Size: 81 797 16 18 29 98
Fragments arranged by size:
1123 797 759 537 233 148 98 81
52 51 29 21 18 16
BanI G'GyrC_C
Cuts at: 0 370 940 1587 1693 1998 2407 2578 2653
Size: 370 570 647 106 305 409 171 75
Cuts at: 2653 3853 3963
Size: 1200 110
Fragments arranged by size:
1200 647 570 409 370 305 171 110
106 75
BanII G_rGCy'C
Cuts at: 0 1150 1655 1682 1712 1772 2497 2684 2925
Size: 1150 505 27 30 60 725 187 241
Cuts at: 2925 2986 3003 3199 3206 3249 3385 3652 3663
Size: 61 17 196 7 43 136 267 11
Cuts at: 3663 3778 3843 3874 3963
Size: 115 65 31 89
Fragments arranged by size:
1150 725 505 267 241 196 187 136
115 89 65 61 60 43 31 30
27 17 11 7
BbeI G_GCGC'C
Cuts at: 0 2002 3963
Size: 2002 1961
BbsI GAAGACnn'nnnn_
Cuts at: 0 2819 3963
Size: 2819 1144
BbvCI CC'TCA_GC
Cuts at: 0 1598 2600 3963
Size: 1598 1002 1363
Fragments arranged by size:
1598 1363 1002
BciVI GTATCCnnnnn_n'
Cuts at: 0 3175 3963
Size: 3175 788
BclI T'GATC_A
Cuts at: 0 749 863 3963
Size: 749 114 3100
Fragments arranged by size:
3100 749 114
BglI GCCn_nnn'nGGC
Cuts at: 0 1128 2464 2557 2694 3322 3479 3963
Size: 1128 1336 93 137 628 157 484
Fragments arranged by size:
1336 1128 628 484 157 137 93
BglII A'GATC_T
Cuts at: 0 140 410 3963
Size: 140 270 3553
Fragments arranged by size:
3553 270 140
BlpI GC'TnA_GC
Cuts at: 0 382 1676 1858 3905 3963
Size: 382 1294 182 2047 58
Fragments arranged by size:
2047 1294 382 182 58
Bme1580I G_kGCm'C
Cuts at: 0 1150 1311 1696 2412 2581 2795 2925 3003
Size: 1150 161 385 716 169 214 130 78
Cuts at: 3003 3652 3843 3963
Size: 649 191 120
Fragments arranged by size:
1150 716 649 385 214 191 169 161
130 120 78
BmgBI CAC'GTC
Cuts at: 0 1539 3963
Size: 1539 2424
BmrI ACTGGGnnnn_n'
Cuts at: 0 936 1145 1294 1327 3963
Size: 936 209 149 33 2636
Fragments arranged by size:
2636 936 209 149 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2511 2910 3387 3795 3913 3963
Size: 2511 399 477 408 118 50
Fragments arranged by size:
2511 477 408 399 118 50
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 735 1652 2346 2500 2905 2956 3109
Size: 310 425 917 694 154 405 51 153
Cuts at: 3109 3321 3559 3883 3963
Size: 212 238 324 80
Fragments arranged by size:
917 694 425 405 324 310 238 212
154 153 80 51
Bpu10I CC'TnA_GC
Cuts at: 0 1567 1598 2600 3963
Size: 1567 31 1002 1363
Fragments arranged by size:
1567 1363 1002 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 205 226 1639 2656 3963
Size: 169 36 21 1413 1017 1307
Fragments arranged by size:
1413 1307 1017 169 36 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 2063 3358 3963
Size: 2063 1295 605
Fragments arranged by size:
2063 1295 605
BsaAI yAC'GTr
Cuts at: 0 84 3963
Size: 84 3879
BsaBI GATnn'nnATC
Cuts at: 0 1237 3963
Size: 1237 2726
BsaHI Gr'CG_yC
Cuts at: 0 1030 1885 1999 3129 3586 3824 3963
Size: 1030 855 114 1130 457 238 139
Fragments arranged by size:
1130 1030 855 457 238 139 114
BsaWI w'CCGG_w
Cuts at: 0 770 1217 1229 1352 2114 2393 2830 3963
Size: 770 447 12 123 762 279 437 1133
Fragments arranged by size:
1133 770 762 447 437 279 123 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1216 1276 1434 1458 1615 1618 1720 1729
Size: 1216 60 158 24 157 3 102 9
Cuts at: 1729 1750 1828 2218 2221 2224 2227 2272 2287
Size: 21 78 390 3 3 3 45 15
Cuts at: 2287 2290 2302 2305 2308 2317 2320 2323 2397
Size: 3 12 3 3 9 3 3 74
Cuts at: 2397 2503 2588 2748 2869 2929 3199 3324 3358
Size: 106 85 160 121 60 270 125 34
Cuts at: 3358 3403 3963
Size: 45 560
Fragments arranged by size:
1216 560 390 270 160 158 157 125
121 106 102 85 78 74 60 60
45 45 34 24 21 15 12 9
9 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 200 399 980 1143 1291 1681 1758 1838
Size: 200 199 581 163 148 390 77 80
Cuts at: 1838 1853 2343 2615 2709 2866 2877 3509 3543
Size: 15 490 272 94 157 11 632 34
Cuts at: 3543 3681 3842 3952 3963
Size: 138 161 110 11
Fragments arranged by size:
632 581 490 390 272 200 199 163
161 157 148 138 110 94 80 77
34 15 11 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 436 1912 3963
Size: 291 145 1476 2051
Fragments arranged by size:
2051 1476 291 145
BsiEI CG_ry'CG
Cuts at: 0 17 3253 3963
Size: 17 3236 710
Fragments arranged by size:
3236 710 17
BsiHKAI G_wGCw'C
Cuts at: 0 242 388 770 967 1088 1655 2389 2795
Size: 242 146 382 197 121 567 734 406
Cuts at: 2795 3963
Size: 1168
Fragments arranged by size:
1168 734 567 406 382 242 197 146
121
BsmI GAATG_Cn'
Cuts at: 0 1110 3963
Size: 1110 2853
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1639 1876 3963
Size: 1639 237 2087
Fragments arranged by size:
2087 1639 237
Bsp1286I G_dGCh'C
Cuts at: 0 242 388 770 967 1088 1150 1311 1655
Size: 242 146 382 197 121 62 161 344
Cuts at: 1655 1682 1696 1712 1772 2389 2412 2497 2581
Size: 27 14 16 60 617 23 85 84
Cuts at: 2581 2684 2795 2925 2986 3003 3199 3206 3249
Size: 103 111 130 61 17 196 7 43
Cuts at: 3249 3385 3652 3663 3778 3843 3874 3963
Size: 136 267 11 115 65 31 89
Fragments arranged by size:
617 382 344 267 242 197 196 161
146 136 130 121 115 111 103 89
85 84 65 62 61 60 43 31
27 23 17 16 14 11 7
BspEI T'CCGG_A
Cuts at: 0 1229 2114 3963
Size: 1229 885 1849
Fragments arranged by size:
1849 1229 885
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 204 263 356 1055 1829 3963
Size: 167 37 59 93 699 774 2134
Fragments arranged by size:
2134 774 699 167 93 59 37
BsrBI CCG'CTC
Cuts at: 0 1583 1749 2610 3231 3437 3963
Size: 1583 166 861 621 206 526
Fragments arranged by size:
1583 861 621 526 206 166
BsrDI GCAATG_nn'
Cuts at: 0 391 821 902 2795 3735 3963
Size: 391 430 81 1893 940 228
Fragments arranged by size:
1893 940 430 391 228 81
BsrFI r'CCGG_y
Cuts at: 0 72 3411 3831 3963
Size: 72 3339 420 132
Fragments arranged by size:
3339 420 132 72
BssHII G'CGCG_C
Cuts at: 0 3724 3909 3963
Size: 3724 185 54
Fragments arranged by size:
3724 185 54
BssSI C'ACGA_G
Cuts at: 0 960 2027 3963
Size: 960 1067 1936
Fragments arranged by size:
1936 1067 960
BstAPI GCAn_nnn'nTGC
Cuts at: 0 774 1830 2139 3963
Size: 774 1056 309 1824
Fragments arranged by size:
1824 1056 774 309
BstXI CCAn_nnnn'nTGG
Cuts at: 0 84 1870 2424 2505 3850 3963
Size: 84 1786 554 81 1345 113
Fragments arranged by size:
1786 1345 554 113 84 81
BstYI r'GATC_y
Cuts at: 0 140 410 1434 3526 3963
Size: 140 270 1024 2092 437
Fragments arranged by size:
2092 1024 437 270 140
Bsu36I CC'TnA_GG
Cuts at: 0 1184 2180 2486 3207 3318 3963
Size: 1184 996 306 721 111 645
Fragments arranged by size:
1184 996 721 645 306 111
BtgI C'CryG_G
Cuts at: 0 152 482 557 1388 1697 1994 2462 2621
Size: 152 330 75 831 309 297 468 159
Cuts at: 2621 3531 3639 3963
Size: 910 108 324
Fragments arranged by size:
910 831 468 330 324 309 297 159
152 108 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1883 3963
Size: 1883 2080
BtsI GCAGTG_nn'
Cuts at: 0 10 704 1726 2373 2546 3037 3602 3963
Size: 10 694 1022 647 173 491 565 361
Fragments arranged by size:
1022 694 647 565 491 361 173 10
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 24 59 3963
Size: 24 35 3904
Fragments arranged by size:
3904 35 24
DraIII CAC_nnn'GTG
Cuts at: 0 441 3963
Size: 441 3522
EaeI y'GGCC_r
Cuts at: 0 14 74 233 341 587 1527 1723 1872
Size: 14 60 159 108 246 940 196 149
Cuts at: 1872 2465 3026 3110 3221 3250 3599 3636 3963
Size: 593 561 84 111 29 349 37 327
Fragments arranged by size:
940 593 561 349 327 246 196 159
149 111 108 84 60 37 29 14
EagI C'GGCC_G
Cuts at: 0 14 3250 3963
Size: 14 3236 713
Fragments arranged by size:
3236 713 14
EarI CTCTTCn'nnn_
Cuts at: 0 679 814 1739 2195 2222 2231 2249 2258
Size: 679 135 925 456 27 9 18 9
Cuts at: 2258 2264 2279 2294 3280 3427 3963
Size: 6 15 15 986 147 536
Fragments arranged by size:
986 925 679 536 456 147 135 27
18 15 15 9 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1408 1825 3607 3708 3963
Size: 1408 417 1782 101 255
Fragments arranged by size:
1782 1408 417 255 101
EcoICRI GAG'CTC
Cuts at: 0 1653 3963
Size: 1653 2310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 735 802 1211 1652 2346 2500 2905
Size: 310 425 67 409 441 694 154 405
Cuts at: 2905 2956 3109 3321 3559 3883 3963
Size: 51 153 212 238 324 80
Fragments arranged by size:
694 441 425 409 405 324 310 238
212 154 153 80 67 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2702 3426 3963
Size: 2702 724 537
Fragments arranged by size:
2702 724 537
EcoO109I rG'GnC_Cy
Cuts at: 0 216 1044 1130 1147 1180 1287 1563 1818
Size: 216 828 86 17 33 107 276 255
Cuts at: 1818 1936 2157 2347 2475 2695 2783 2921 2999
Size: 118 221 190 128 220 88 138 78
Cuts at: 2999 3000 3261 3323 3677 3807 3883 3963
Size: 1 261 62 354 130 76 80
Fragments arranged by size:
828 354 276 261 255 221 220 216
190 138 130 128 118 107 88 86
80 78 76 62 33 17 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 429 3963
Size: 429 3534
HaeII r_GCGC'y
Cuts at: 0 1807 2002 2867 2909 2932 3354 3688 3963
Size: 1807 195 865 42 23 422 334 275
Fragments arranged by size:
1807 865 422 334 275 195 42 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 652 684 1408 1440 3181 3213 3963
Size: 652 32 724 32 1741 32 750
Fragments arranged by size:
1741 750 724 652 32 32 32
HincII GTy'rAC
Cuts at: 0 1215 3963
Size: 1215 2748
KasI G'GCGC_C
Cuts at: 0 1998 3963
Size: 1998 1965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1940 3963
Size: 1940 2023
MscI TGG'CCA
Cuts at: 0 3028 3112 3963
Size: 3028 84 851
Fragments arranged by size:
3028 851 84
MslI CAynn'nnrTG
Cuts at: 0 685 871 1091 1537 3493 3963
Size: 685 186 220 446 1956 470
Fragments arranged by size:
1956 685 470 446 220 186
MspA1I CmG'CkG
Cuts at: 0 708 1593 1857 2320 2634 3087 3418 3579
Size: 708 885 264 463 314 453 331 161
Cuts at: 3579 3641 3963
Size: 62 322
Fragments arranged by size:
885 708 463 453 331 322 314 264
161 62
NaeI GCC'GGC
Cuts at: 0 74 3413 3833 3963
Size: 74 3339 420 130
Fragments arranged by size:
3339 420 130 74
NarI GG'CG_CC
Cuts at: 0 1999 3963
Size: 1999 1964
NcoI C'CATG_G
Cuts at: 0 152 482 2462 3531 3963
Size: 152 330 1980 1069 432
Fragments arranged by size:
1980 1069 432 330 152
NgoMIV G'CCGG_C
Cuts at: 0 72 3411 3831 3963
Size: 72 3339 420 132
Fragments arranged by size:
3339 420 132 72
NotI GC'GGCC_GC
Cuts at: 0 14 3963
Size: 14 3949
NspI r_CATG'y
Cuts at: 0 684 792 1800 2139 3492 3963
Size: 684 108 1008 339 1353 471
Fragments arranged by size:
1353 1008 684 471 339 108
PasI CC'CwG_GG
Cuts at: 0 3592 3652 3963
Size: 3592 60 311
Fragments arranged by size:
3592 311 60
PflMI CCAn_nnn'nTGG
Cuts at: 0 441 1039 3963
Size: 441 598 2924
Fragments arranged by size:
2924 598 441
PfoI T'CCnGG_A
Cuts at: 0 812 1052 3963
Size: 812 240 2911
Fragments arranged by size:
2911 812 240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 590 622 3963
Size: 590 32 3341
Fragments arranged by size:
3341 590 32
PpuMI rG'GwC_Cy
Cuts at: 0 216 1044 1180 1287 1818 1936 2157 2475
Size: 216 828 136 107 531 118 221 318
Cuts at: 2475 2783 3261 3807 3883 3963
Size: 308 478 546 76 80
Fragments arranged by size:
828 546 531 478 318 308 221 216
136 118 107 80 76
PshAI GACnn'nnGTC
Cuts at: 0 1210 3963
Size: 1210 2753
PspOMI G'GGCC_C
Cuts at: 0 1146 2921 2999 3648 3839 3963
Size: 1146 1775 78 649 191 124
Fragments arranged by size:
1775 1146 649 191 124 78
PstI C_TGCA'G
Cuts at: 0 1826 2319 3584 3963
Size: 1826 493 1265 379
Fragments arranged by size:
1826 1265 493 379
PvuII CAG'CTG
Cuts at: 0 708 1857 2634 3579 3963
Size: 708 1149 777 945 384
Fragments arranged by size:
1149 945 777 708 384
SacI G_AGCT'C
Cuts at: 0 1655 3963
Size: 1655 2308
SacII CC_GC'GG
Cuts at: 0 3642 3963
Size: 3642 321
SanDI GG'GwC_CC
Cuts at: 0 1287 1936 3807 3963
Size: 1287 649 1871 156
Fragments arranged by size:
1871 1287 649 156
SapI GCTCTTCn'nnn_
Cuts at: 0 1739 3427 3963
Size: 1739 1688 536
Fragments arranged by size:
1739 1688 536
SfcI C'TryA_G
Cuts at: 0 504 1075 1822 2315 3580 3963
Size: 504 571 747 493 1265 383
Fragments arranged by size:
1265 747 571 504 493 383
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2464 3322 3963
Size: 2464 858 641
Fragments arranged by size:
2464 858 641
SfoI GGC'GCC
Cuts at: 0 2000 3963
Size: 2000 1963
SmaI CCC'GGG
Cuts at: 0 1125 1176 1786 2693 3007 3804 3820 3838
Size: 1125 51 610 907 314 797 16 18
Cuts at: 3838 3867 3963
Size: 29 96
Fragments arranged by size:
1125 907 797 610 314 96 51 29
18 16
SmlI C'TyrA_G
Cuts at: 0 22 184 220 241 1654 2671 3963
Size: 22 162 36 21 1413 1017 1292
Fragments arranged by size:
1413 1292 1017 162 36 22 21
SphI G_CATG'C
Cuts at: 0 1800 3492 3963
Size: 1800 1692 471
Fragments arranged by size:
1800 1692 471
SrfI GCCC'GGGC
Cuts at: 0 3838 3963
Size: 3838 125
StuI AGG'CCT
Cuts at: 0 919 1462 3963
Size: 919 543 2501
Fragments arranged by size:
2501 919 543
StyI C'CwwG_G
Cuts at: 0 152 482 2330 2462 3531 3963
Size: 152 330 1848 132 1069 432
Fragments arranged by size:
1848 1069 432 330 152 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1529 3963
Size: 1529 2434
TatI w'GTAC_w
Cuts at: 0 545 3963
Size: 545 3418
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 253 285 3963
Size: 253 32 3678
Fragments arranged by size:
3678 253 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 396 438 1014 1530 3963
Size: 174 222 42 576 516 2433
Fragments arranged by size:
2433 576 516 222 174 42
XmaI C'CCGG_G
Cuts at: 0 1123 1174 1784 2691 3005 3802 3818 3836
Size: 1123 51 610 907 314 797 16 18
Cuts at: 3836 3865 3963
Size: 29 98
Fragments arranged by size:
1123 907 797 610 314 98 51 29
18 16
ZraI GAC'GTC
Cuts at: 0 1886 3963
Size: 1886 2077
Enzymes that do cut:
AatII AcuI AfeI AflII AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PasI PflMI PfoI PpiI PpuMI PshAI PspOMI
PstI PvuII SacI SacII SanDI SapI SfcI SfiI
SfoI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AgeI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BfrBI BmtI
BsaXI BsiWI BspHI BsrGI BstBI BstEII BstZ17I ClaI
DraI DrdI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NdeI NheI NruI NsiI
PacI PciI PmeI PmlI PsiI PspXI PsrI PvuI
RsrII SalI SbfI ScaI SexAI SgfI SgrAI SnaBI
SpeI SspI SwaI Tth111I XbaI XhoI XmnI