Restriction map for
pF1KE3491
(Linear) (Six-base) MAPSORT of: KIEE3491.seq Check: 8336 from: 1 to: 5595
KIEE3491.orf
With 182 enzymes: SgfI *
March 10, 2017 18:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2313 5595
Size: 2313 3282
AatII G_ACGT'C
Cuts at: 0 1487 5595
Size: 1487 4108
AccI GT'mk_AC
Cuts at: 0 1823 3236 5595
Size: 1823 1413 2359
Fragments arranged by size:
2359 1823 1413
AclI AA'CG_TT
Cuts at: 0 1593 5595
Size: 1593 4002
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 895 2009 2076 2158 2630 2741 4619
Size: 371 524 1114 67 82 472 111 1878
Cuts at: 4619 5400 5491 5507 5521 5595
Size: 781 91 16 14 74
Fragments arranged by size:
1878 1114 781 524 472 371 111 91
82 74 67 16 14
AfeI AGC'GCT
Cuts at: 0 1849 5595
Size: 1849 3746
AflII C'TTAA_G
Cuts at: 0 2164 5595
Size: 2164 3431
AflIII A'CryG_T
Cuts at: 0 546 2576 5595
Size: 546 2030 3019
Fragments arranged by size:
3019 2030 546
AleI CACnn'nnGTG
Cuts at: 0 535 5595
Size: 535 5060
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4449 4481 4895 4927 5595
Size: 4449 32 414 32 668
Fragments arranged by size:
4449 668 414 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1302 4042 5595
Size: 1302 2740 1553
Fragments arranged by size:
2740 1553 1302
ApoI r'AATT_y
Cuts at: 0 101 285 433 821 1399 2265 2563 3562
Size: 101 184 148 388 578 866 298 999
Cuts at: 3562 3587 4024 4198 4306 5021 5176 5319 5547
Size: 25 437 174 108 715 155 143 228
Cuts at: 5547 5595
Size: 48
Fragments arranged by size:
999 866 715 578 437 388 298 228
184 174 155 148 143 108 101 48
25
AseI AT'TA_AT
Cuts at: 0 809 1603 2887 5595
Size: 809 794 1284 2708
Fragments arranged by size:
2708 1284 809 794
BamHI G'GATC_C
Cuts at: 0 1309 5595
Size: 1309 4286
BanI G'GyrC_C
Cuts at: 0 4057 5595
Size: 4057 1538
BanII G_rGCy'C
Cuts at: 0 262 1031 5595
Size: 262 769 4564
Fragments arranged by size:
4564 769 262
BbsI GAAGACnn'nnnn_
Cuts at: 0 4523 4790 5595
Size: 4523 267 805
Fragments arranged by size:
4523 805 267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 578 612 5595
Size: 578 34 4983
Fragments arranged by size:
4983 578 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4641 5595
Size: 4641 954
BfrBI ATG'CAT
Cuts at: 0 1213 1395 2854 3148 5595
Size: 1213 182 1459 294 2447
Fragments arranged by size:
2447 1459 1213 294 182
BglII A'GATC_T
Cuts at: 0 966 1970 2708 3708 5466 5595
Size: 966 1004 738 1000 1758 129
Fragments arranged by size:
1758 1004 1000 966 738 129
BmgBI CAC'GTC
Cuts at: 0 3209 5595
Size: 3209 2386
BmrI ACTGGGnnnn_n'
Cuts at: 0 3969 5595
Size: 3969 1626
BmtI G_CTAG'C
Cuts at: 0 976 3773 5248 5595
Size: 976 2797 1475 347
Fragments arranged by size:
2797 1475 976 347
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 577 5595
Size: 577 5018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 539 1490 1638 2166 4070 5192 5595
Size: 521 18 951 148 528 1904 1122 403
Fragments arranged by size:
1904 1122 951 528 521 403 148 18
Bpu10I CC'TnA_GC
Cuts at: 0 694 5595
Size: 694 4901
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2348 3893 5595
Size: 2348 1545 1702
Fragments arranged by size:
2348 1702 1545
BsaI GGTCTCn'nnnn_
Cuts at: 0 4624 5595
Size: 4624 971
BsaBI GATnn'nnATC
Cuts at: 0 1160 2372 5531 5595
Size: 1160 1212 3159 64
Fragments arranged by size:
3159 1212 1160 64
BsaHI Gr'CG_yC
Cuts at: 0 1484 5595
Size: 1484 4111
BsaWI w'CCGG_w
Cuts at: 0 4554 5595
Size: 4554 1041
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3635 3665 4304 4334 5595
Size: 3635 30 639 30 1261
Fragments arranged by size:
3635 1261 639 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 536 1655 3377 3888 4864 4874 5595
Size: 536 1119 1722 511 976 10 721
Fragments arranged by size:
1722 1119 976 721 536 511 10
BseYI C'CCAG_C
Cuts at: 0 2725 5382 5595
Size: 2725 2657 213
Fragments arranged by size:
2725 2657 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 5595
Size: 1175 4420
BsmI GAATG_Cn'
Cuts at: 0 723 3623 4706 5595
Size: 723 2900 1083 889
Fragments arranged by size:
2900 1083 889 723
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1475 5595
Size: 1475 4120
Bsp1286I G_dGCh'C
Cuts at: 0 262 1031 5595
Size: 262 769 4564
Fragments arranged by size:
4564 769 262
BspHI T'CATG_A
Cuts at: 0 1683 3072 3366 3510 4524 5595
Size: 1683 1389 294 144 1014 1071
Fragments arranged by size:
1683 1389 1071 1014 294 144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1893 2167 2313 4011 5595
Size: 1893 274 146 1698 1584
Fragments arranged by size:
1893 1698 1584 274 146
BsrDI GCAATG_nn'
Cuts at: 0 1262 5595
Size: 1262 4333
BsrGI T'GTAC_A
Cuts at: 0 414 2212 2625 3246 5595
Size: 414 1798 413 621 2349
Fragments arranged by size:
2349 1798 621 414 413
BssSI C'ACGA_G
Cuts at: 0 3794 5595
Size: 3794 1801
BstAPI GCAn_nnn'nTGC
Cuts at: 0 172 369 1960 5595
Size: 172 197 1591 3635
Fragments arranged by size:
3635 1591 197 172
BstEII G'GTnAC_C
Cuts at: 0 202 5595
Size: 202 5393
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3819 5595
Size: 3819 1776
BstYI r'GATC_y
Cuts at: 0 966 1309 1970 2426 2708 3708 3969 5359
Size: 966 343 661 456 282 1000 261 1390
Cuts at: 5359 5466 5595
Size: 107 129
Fragments arranged by size:
1390 1000 966 661 456 343 282 261
129 107
Bsu36I CC'TnA_GG
Cuts at: 0 5510 5595
Size: 5510 85
BtgI C'CryG_G
Cuts at: 0 616 2108 2432 5595
Size: 616 1492 324 3163
Fragments arranged by size:
3163 1492 616 324
BtsI GCAGTG_nn'
Cuts at: 0 1192 1303 3532 3746 5595
Size: 1192 111 2229 214 1849
Fragments arranged by size:
2229 1849 1192 214 111
DraI TTT'AAA
Cuts at: 0 2445 4356 5595
Size: 2445 1911 1239
Fragments arranged by size:
2445 1911 1239
EaeI y'GGCC_r
Cuts at: 0 837 1068 1641 2066 2990 3696 5595
Size: 837 231 573 425 924 706 1899
Fragments arranged by size:
1899 924 837 706 573 425 231
EarI CTCTTCn'nnn_
Cuts at: 0 783 1891 2417 3515 3575 3908 4056 4757
Size: 783 1108 526 1098 60 333 148 701
Cuts at: 4757 4866 5228 5467 5595
Size: 109 362 239 128
Fragments arranged by size:
1108 1098 783 701 526 362 333 239
148 128 109 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 787 5595
Size: 787 4808
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 521 539 895 1490 1638 2009 2076
Size: 371 150 18 356 595 148 371 67
Cuts at: 2076 2158 2166 2630 2741 4070 4619 5192 5400
Size: 82 8 464 111 1329 549 573 208
Cuts at: 5400 5491 5507 5521 5595
Size: 91 16 14 74
Fragments arranged by size:
1329 595 573 549 464 371 371 356
208 150 148 111 91 82 74 67
18 16 14 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1584 4730 5595
Size: 1584 3146 865
Fragments arranged by size:
3146 1584 865
EcoO109I rG'GnC_Cy
Cuts at: 0 72 2959 5595
Size: 72 2887 2636
Fragments arranged by size:
2887 2636 72
EcoRI G'AATT_C
Cuts at: 0 821 3587 5595
Size: 821 2766 2008
Fragments arranged by size:
2766 2008 821
EcoRV GAT'ATC
Cuts at: 0 4911 5595
Size: 4911 684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1345 3934 5595
Size: 1345 2589 1661
Fragments arranged by size:
2589 1661 1345
HaeII r_GCGC'y
Cuts at: 0 1851 5595
Size: 1851 3744
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1502 1534 3006 3038 3635 3667 4561 4593
Size: 1502 32 1472 32 597 32 894 32
Cuts at: 4593 5595
Size: 1002
Fragments arranged by size:
1502 1472 1002 894 597 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1260 3215 3654 5595
Size: 1260 1955 439 1941
Fragments arranged by size:
1955 1941 1260 439
HindIII A'AGCT_T
Cuts at: 0 2512 5047 5452 5595
Size: 2512 2535 405 143
Fragments arranged by size:
2535 2512 405 143
MfeI C'AATT_G
Cuts at: 0 2555 2786 5595
Size: 2555 231 2809
Fragments arranged by size:
2809 2555 231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 738 1287 1474 3621 3743 5242 5595
Size: 531 207 549 187 2147 122 1499 353
Fragments arranged by size:
2147 1499 549 531 353 207 187 122
MscI TGG'CCA
Cuts at: 0 839 1070 2992 3698 5595
Size: 839 231 1922 706 1897
Fragments arranged by size:
1922 1897 839 706 231
MslI CAynn'nnrTG
Cuts at: 0 535 739 1245 2113 2437 3010 5595
Size: 535 204 506 868 324 573 2585
Fragments arranged by size:
2585 868 573 535 506 324 204
MspA1I CmG'CkG
Cuts at: 0 4669 5595
Size: 4669 926
NcoI C'CATG_G
Cuts at: 0 616 2108 2432 5595
Size: 616 1492 324 3163
Fragments arranged by size:
3163 1492 616 324
NdeI CA'TA_TG
Cuts at: 0 336 1989 2856 3433 5595
Size: 336 1653 867 577 2162
Fragments arranged by size:
2162 1653 867 577 336
NheI G'CTAG_C
Cuts at: 0 972 3769 5244 5595
Size: 972 2797 1475 351
Fragments arranged by size:
2797 1475 972 351
NsiI A_TGCA'T
Cuts at: 0 1215 1397 2856 3150 5595
Size: 1215 182 1459 294 2445
Fragments arranged by size:
2445 1459 1215 294 182
NspI r_CATG'y
Cuts at: 0 154 429 550 574 1395 4797 5595
Size: 154 275 121 24 821 3402 798
Fragments arranged by size:
3402 821 798 275 154 121 24
PciI A'CATG_T
Cuts at: 0 546 5595
Size: 546 5049
PflMI CCAn_nnn'nTGG
Cuts at: 0 4042 5595
Size: 4042 1553
PfoI T'CCnGG_A
Cuts at: 0 13 90 1506 5595
Size: 13 77 1416 4089
Fragments arranged by size:
4089 1416 77 13
PpuMI rG'GwC_Cy
Cuts at: 0 72 2959 5595
Size: 72 2887 2636
Fragments arranged by size:
2887 2636 72
PsiI TTA'TAA
Cuts at: 0 4985 5595
Size: 4985 610
PstI C_TGCA'G
Cuts at: 0 492 966 4008 5595
Size: 492 474 3042 1587
Fragments arranged by size:
3042 1587 492 474
PvuII CAG'CTG
Cuts at: 0 4669 5595
Size: 4669 926
SapI GCTCTTCn'nnn_
Cuts at: 0 3515 4866 5595
Size: 3515 1351 729
Fragments arranged by size:
3515 1351 729
SexAI A'CCwGG_T
Cuts at: 0 2009 4894 5595
Size: 2009 2885 701
Fragments arranged by size:
2885 2009 701
SfcI C'TryA_G
Cuts at: 0 309 488 962 1569 4004 4286 5445 5595
Size: 309 179 474 607 2435 282 1159 150
Fragments arranged by size:
2435 1159 607 474 309 282 179 150
SmlI C'TyrA_G
Cuts at: 0 2164 2363 3908 5595
Size: 2164 199 1545 1687
Fragments arranged by size:
2164 1687 1545 199
SphI G_CATG'C
Cuts at: 0 429 1395 5595
Size: 429 966 4200
Fragments arranged by size:
4200 966 429
SspI AAT'ATT
Cuts at: 0 2909 3324 3948 4130 5595
Size: 2909 415 624 182 1465
Fragments arranged by size:
2909 1465 624 415 182
StuI AGG'CCT
Cuts at: 0 4817 5595
Size: 4817 778
StyI C'CwwG_G
Cuts at: 0 68 616 1313 2108 2432 5098 5595
Size: 68 548 697 795 324 2666 497
Fragments arranged by size:
2666 795 697 548 497 324 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 190 461 3131 4788 5595
Size: 190 271 2670 1657 807
Fragments arranged by size:
2670 1657 807 271 190
TatI w'GTAC_w
Cuts at: 0 414 2212 2625 3246 4210 4884 5595
Size: 414 1798 413 621 964 674 711
Fragments arranged by size:
1798 964 711 674 621 414 413
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2972 3004 3604 3636 4444 4476 5595
Size: 2972 32 600 32 808 32 1119
Fragments arranged by size:
2972 1119 808 600 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1416 4246 5595
Size: 1416 2830 1349
Fragments arranged by size:
2830 1416 1349
XmnI GAAnn'nnTTC
Cuts at: 0 356 908 1082 5595
Size: 356 552 174 4513
Fragments arranged by size:
4513 552 356 174
ZraI GAC'GTC
Cuts at: 0 1485 5595
Size: 1485 4110
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AleI AloI AlwNI ApoI AseI BamHI BanI BanII
BbsI BcgI BciVI BfrBI BglII BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI
NheI NsiI NspI PciI PflMI PfoI PpuMI PsiI
PstI PvuII SapI SexAI SfcI SmlI SphI SspI
StuI StyI TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AgeI AhdI AlfI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BbeI BbvCI BclI BglI BlpI
Bme1580I BsaAI BsiEI BsiHKAI BsiWI BspEI BsrBI BsrFI
BssHII BstBI BstZ17I BtgZI ClaI CspCI DraIII DrdI
EagI EcoICRI FseI FspI FspAI HpaI KasI KpnI
MluI NaeI NarI NgoMIV NotI NruI PacI PasI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI
Tth111I XbaI XhoI XmaI