Restriction map for
pF1KE3523
(Linear) (Six-base) MAPSORT of: KIEE3523.seq Check: 8744 from: 1 to: 1047
KIEE3523.orf
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
Acc65I G'GTAC_C
Cuts at: 0 638 1047
Size: 638 409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 602 941 947 1047
Size: 325 277 339 6 100
Fragments arranged by size:
339 325 277 100 6
AflIII A'CryG_T
Cuts at: 0 501 1047
Size: 501 546
AvaI C'yCGr_G
Cuts at: 0 584 1047
Size: 584 463
BanI G'GyrC_C
Cuts at: 0 638 1047
Size: 638 409
BanII G_rGCy'C
Cuts at: 0 560 1047
Size: 560 487
BbsI GAAGACnn'nnnn_
Cuts at: 0 935 1047
Size: 935 112
BciVI GTATCCnnnnn_n'
Cuts at: 0 807 1047
Size: 807 240
Bme1580I G_kGCm'C
Cuts at: 0 42 1047
Size: 42 1005
BmrI ACTGGGnnnn_n'
Cuts at: 0 399 1047
Size: 399 648
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1047
Size: 796 251
BsaI GGTCTCn'nnnn_
Cuts at: 0 959 1047
Size: 959 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 60 90 922 952 958 988 1047
Size: 60 30 832 30 6 30 59
Fragments arranged by size:
832 60 59 30 30 30 6
BseYI C'CCAG_C
Cuts at: 0 171 601 660 795 906 1047
Size: 171 430 59 135 111 141
Fragments arranged by size:
430 171 141 135 111 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 1047
Size: 722 325
BsiHKAI G_wGCw'C
Cuts at: 0 335 560 1047
Size: 335 225 487
Fragments arranged by size:
487 335 225
BsmI GAATG_Cn'
Cuts at: 0 261 1047
Size: 261 786
BsmBI CGTCTCn'nnnn_
Cuts at: 0 978 1047
Size: 978 69
Bsp1286I G_dGCh'C
Cuts at: 0 42 335 560 1047
Size: 42 293 225 487
Fragments arranged by size:
487 293 225 42
BspHI T'CATG_A
Cuts at: 0 996 1047
Size: 996 51
BsrFI r'CCGG_y
Cuts at: 0 641 1047
Size: 641 406
BstYI r'GATC_y
Cuts at: 0 96 1047
Size: 96 951
BtgI C'CryG_G
Cuts at: 0 64 1047
Size: 64 983
BtsI GCAGTG_nn'
Cuts at: 0 865 1047
Size: 865 182
DraI TTT'AAA
Cuts at: 0 239 1047
Size: 239 808
EaeI y'GGCC_r
Cuts at: 0 820 1047
Size: 820 227
EarI CTCTTCn'nnn_
Cuts at: 0 86 118 621 1047
Size: 86 32 503 426
Fragments arranged by size:
503 426 86 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 966 1047
Size: 966 81
EcoICRI GAG'CTC
Cuts at: 0 558 1047
Size: 558 489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 602 796 941 947 1047
Size: 325 277 194 145 6 100
Fragments arranged by size:
325 277 194 145 100 6
EcoO109I rG'GnC_Cy
Cuts at: 0 477 516 1047
Size: 477 39 531
Fragments arranged by size:
531 477 39
HaeII r_GCGC'y
Cuts at: 0 1013 1047
Size: 1013 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 922 954 956 958 988 990 1047
Size: 922 32 2 2 30 2 57
Fragments arranged by size:
922 57 32 30 2 2 2
HincII GTy'rAC
Cuts at: 0 736 1047
Size: 736 311
HindIII A'AGCT_T
Cuts at: 0 293 1047
Size: 293 754
KpnI G_GTAC'C
Cuts at: 0 642 1047
Size: 642 405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1047
Size: 782 265
MslI CAynn'nnrTG
Cuts at: 0 250 1047
Size: 250 797
MspA1I CmG'CkG
Cuts at: 0 774 1026 1047
Size: 774 252 21
Fragments arranged by size:
774 252 21
NdeI CA'TA_TG
Cuts at: 0 694 1047
Size: 694 353
NspI r_CATG'y
Cuts at: 0 322 390 1047
Size: 322 68 657
Fragments arranged by size:
657 322 68
PasI CC'CwG_GG
Cuts at: 0 17 1047
Size: 17 1030
PfoI T'CCnGG_A
Cuts at: 0 423 1047
Size: 423 624
PpuMI rG'GwC_Cy
Cuts at: 0 516 1047
Size: 516 531
PvuII CAG'CTG
Cuts at: 0 774 1026 1047
Size: 774 252 21
Fragments arranged by size:
774 252 21
SacI G_AGCT'C
Cuts at: 0 560 1047
Size: 560 487
SapI GCTCTTCn'nnn_
Cuts at: 0 118 1047
Size: 118 929
SfcI C'TryA_G
Cuts at: 0 806 1047
Size: 806 241
SmaI CCC'GGG
Cuts at: 0 586 1047
Size: 586 461
SpeI A'CTAG_T
Cuts at: 0 228 1047
Size: 228 819
SrfI GCCC'GGGC
Cuts at: 0 586 1047
Size: 586 461
SspI AAT'ATT
Cuts at: 0 327 676 1047
Size: 327 349 371
Fragments arranged by size:
371 349 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 1047
Size: 163 884
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 60 92 215 247 1047
Size: 60 32 123 32 800
Fragments arranged by size:
800 123 60 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 970 1047
Size: 970 77
XmaI C'CCGG_G
Cuts at: 0 584 1047
Size: 584 463
Enzymes that do cut:
Acc65I AcuI AflIII AvaI BanI BanII BbsI BciVI
Bme1580I BmrI BpmI BsaI BsaXI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BsrFI BstYI BtgI BtsI
DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII
Hin4I HincII HindIII KpnI MmeI MslI MspA1I NdeI
NspI PasI PfoI PpuMI PvuII SacI SapI SfcI
SmaI SpeI SrfI SspI TaqII TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI
BsiWI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HpaI KasI MfeI MluI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SphI StuI StyI SwaI TatI XbaI
XcmI XhoI XmnI ZraI