Restriction map for
pF1KE3542
(Linear) (Six-base) MAPSORT of: KIEE3542.seq Check: 5790 from: 1 to: 3390
KIEE3542.orf
With 182 enzymes: SgfI *
March 22, 2013 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 84 232 1686 2188 3390
Size: 84 148 1454 502 1202
Fragments arranged by size:
1454 1202 502 148 84
AccI GT'mk_AC
Cuts at: 0 715 3390
Size: 715 2675
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1262 3390
Size: 1022 240 2128
Fragments arranged by size:
2128 1022 240
AflII C'TTAA_G
Cuts at: 0 1795 3390
Size: 1795 1595
AhdI GACnn_n'nnGTC
Cuts at: 0 1497 3325 3390
Size: 1497 1828 65
Fragments arranged by size:
1828 1497 65
AleI CACnn'nnGTG
Cuts at: 0 3109 3390
Size: 3109 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1694 3390
Size: 1694 1696
AlwNI CAG_nnn'CTG
Cuts at: 0 2767 3390
Size: 2767 623
ApoI r'AATT_y
Cuts at: 0 304 578 595 697 797 1867 1873 2143
Size: 304 274 17 102 100 1070 6 270
Cuts at: 2143 2171 2203 2836 3046 3390
Size: 28 32 633 210 344
Fragments arranged by size:
1070 633 344 304 274 270 210 102
100 32 28 17 6
AvaI C'yCGr_G
Cuts at: 0 112 136 2082 3390
Size: 112 24 1946 1308
Fragments arranged by size:
1946 1308 112 24
AvrII C'CTAG_G
Cuts at: 0 271 1285 3390
Size: 271 1014 2105
Fragments arranged by size:
2105 1014 271
BamHI G'GATC_C
Cuts at: 0 2551 3390
Size: 2551 839
BanI G'GyrC_C
Cuts at: 0 39 131 3390
Size: 39 92 3259
Fragments arranged by size:
3259 92 39
BanII G_rGCy'C
Cuts at: 0 34 1339 3390
Size: 34 1305 2051
Fragments arranged by size:
2051 1305 34
BbeI G_GCGC'C
Cuts at: 0 43 135 3390
Size: 43 92 3255
Fragments arranged by size:
3255 92 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 194 2126 2239 3390
Size: 194 1932 113 1151
Fragments arranged by size:
1932 1151 194 113
BbvCI CC'TCA_GC
Cuts at: 0 2844 3390
Size: 2844 546
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 52 86 715 749 3390
Size: 52 34 629 34 2641
Fragments arranged by size:
2641 629 52 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1840 3390
Size: 1840 1550
BclI T'GATC_A
Cuts at: 0 1466 1991 3390
Size: 1466 525 1399
Fragments arranged by size:
1466 1399 525
BfrBI ATG'CAT
Cuts at: 0 2857 3390
Size: 2857 533
BglII A'GATC_T
Cuts at: 0 1365 3390
Size: 1365 2025
Bme1580I G_kGCm'C
Cuts at: 0 106 3099 3390
Size: 106 2993 291
Fragments arranged by size:
2993 291 106
BmgBI CAC'GTC
Cuts at: 0 192 369 3390
Size: 192 177 3021
Fragments arranged by size:
3021 192 177
BmrI ACTGGGnnnn_n'
Cuts at: 0 92 421 1647 1769 2006 2251 3086 3390
Size: 92 329 1226 122 237 245 835 304
Fragments arranged by size:
1226 835 329 304 245 237 122 92
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1577 3390
Size: 1577 1813
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 2724 3390
Size: 2 2722 666
Fragments arranged by size:
2722 666 2
Bpu10I CC'TnA_GC
Cuts at: 0 2844 3390
Size: 2844 546
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2151 3390
Size: 2151 1239
BsaI GGTCTCn'nnnn_
Cuts at: 0 2666 2740 2898 3390
Size: 2666 74 158 492
Fragments arranged by size:
2666 492 158 74
BsaAI yAC'GTr
Cuts at: 0 1506 3390
Size: 1506 1884
BsaHI Gr'CG_yC
Cuts at: 0 40 132 3390
Size: 40 92 3258
Fragments arranged by size:
3258 92 40
BsaWI w'CCGG_w
Cuts at: 0 3153 3390
Size: 3153 237
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 541 571 2875 2905 3390
Size: 541 30 2304 30 485
Fragments arranged by size:
2304 541 485 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 42 48 178 187 190 193 951 1954
Size: 42 6 130 9 3 3 758 1003
Cuts at: 1954 2832 3390
Size: 878 558
Fragments arranged by size:
1003 878 758 558 130 42 9 6
3 3
BseYI C'CCAG_C
Cuts at: 0 1144 2422 3390
Size: 1144 1278 968
Fragments arranged by size:
1278 1144 968
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 3390
Size: 56 3334
BsiHKAI G_wGCw'C
Cuts at: 0 34 3390
Size: 34 3356
BsmI GAATG_Cn'
Cuts at: 0 2618 3390
Size: 2618 772
BsmBI CGTCTCn'nnnn_
Cuts at: 0 375 3312 3390
Size: 375 2937 78
Fragments arranged by size:
2937 375 78
Bsp1286I G_dGCh'C
Cuts at: 0 34 106 1339 3099 3390
Size: 34 72 1233 1760 291
Fragments arranged by size:
1760 1233 291 72 34
BspEI T'CCGG_A
Cuts at: 0 3153 3390
Size: 3153 237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 84 232 1686 2188 3390
Size: 84 148 1454 502 1202
Fragments arranged by size:
1454 1202 502 148 84
BsrDI GCAATG_nn'
Cuts at: 0 863 1538 1655 2624 3390
Size: 863 675 117 969 766
Fragments arranged by size:
969 863 766 675 117
BssSI C'ACGA_G
Cuts at: 0 99 1927 3390
Size: 99 1828 1463
Fragments arranged by size:
1828 1463 99
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2472 2854 3390
Size: 2472 382 536
Fragments arranged by size:
2472 536 382
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2406 2748 3390
Size: 2406 342 642
Fragments arranged by size:
2406 642 342
BstYI r'GATC_y
Cuts at: 0 554 1365 2252 2386 2485 2551 3390
Size: 554 811 887 134 99 66 839
Fragments arranged by size:
887 839 811 554 134 99 66
BstZ17I GTA'TAC
Cuts at: 0 716 3390
Size: 716 2674
Bsu36I CC'TnA_GG
Cuts at: 0 2431 3390
Size: 2431 959
BtgI C'CryG_G
Cuts at: 0 281 625 687 1592 2975 3390
Size: 281 344 62 905 1383 415
Fragments arranged by size:
1383 905 415 344 281 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2808 2867 3390
Size: 2808 59 523
Fragments arranged by size:
2808 523 59
BtsI GCAGTG_nn'
Cuts at: 0 3012 3390
Size: 3012 378
DraI TTT'AAA
Cuts at: 0 2447 3390
Size: 2447 943
DraIII CAC_nnn'GTG
Cuts at: 0 1345 3390
Size: 1345 2045
EaeI y'GGCC_r
Cuts at: 0 349 923 2283 2396 2583 3390
Size: 349 574 1360 113 187 807
Fragments arranged by size:
1360 807 574 349 187 113
EarI CTCTTCn'nnn_
Cuts at: 0 164 988 998 1758 2033 2038 2298 3390
Size: 164 824 10 760 275 5 260 1092
Fragments arranged by size:
1092 824 760 275 260 164 10 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1967 3390
Size: 1967 1423
EcoICRI GAG'CTC
Cuts at: 0 32 3390
Size: 32 3358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 1022 1262 2724 3390
Size: 2 1020 240 1462 666
Fragments arranged by size:
1462 1020 666 240 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 123 3112 3390
Size: 123 2989 278
Fragments arranged by size:
2989 278 123
EcoO109I rG'GnC_Cy
Cuts at: 0 116 201 811 2460 3390
Size: 116 85 610 1649 930
Fragments arranged by size:
1649 930 610 116 85
EcoRI G'AATT_C
Cuts at: 0 2171 3390
Size: 2171 1219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3240 3390
Size: 3240 150
HaeII r_GCGC'y
Cuts at: 0 43 135 3390
Size: 43 92 3255
Fragments arranged by size:
3255 92 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 946 978 1350 1382 1483 1515 1581 1613
Size: 946 32 372 32 101 32 66 32
Cuts at: 1613 1627 1659 1910 1942 1946 1978 2536 2568
Size: 14 32 251 32 4 32 558 32
Cuts at: 2568 2875 2907 3072 3104 3311 3343 3390
Size: 307 32 165 32 207 32 47
Fragments arranged by size:
946 558 372 307 251 207 165 101
66 47 32 32 32 32 32 32
32 32 32 32 32 14 4
HindIII A'AGCT_T
Cuts at: 0 1136 3390
Size: 1136 2254
KasI G'GCGC_C
Cuts at: 0 39 131 3390
Size: 39 92 3259
Fragments arranged by size:
3259 92 39
MfeI C'AATT_G
Cuts at: 0 731 3390
Size: 731 2659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 2589 3390
Size: 398 2191 801
Fragments arranged by size:
2191 801 398
MscI TGG'CCA
Cuts at: 0 351 925 2285 2585 3390
Size: 351 574 1360 300 805
Fragments arranged by size:
1360 805 574 351 300
MslI CAynn'nnrTG
Cuts at: 0 466 1828 2404 2875 3109 3390
Size: 466 1362 576 471 234 281
Fragments arranged by size:
1362 576 471 466 281 234
MspA1I CmG'CkG
Cuts at: 0 70 1743 1945 3003 3390
Size: 70 1673 202 1058 387
Fragments arranged by size:
1673 1058 387 202 70
NarI GG'CG_CC
Cuts at: 0 40 132 3390
Size: 40 92 3258
Fragments arranged by size:
3258 92 40
NcoI C'CATG_G
Cuts at: 0 281 625 2975 3390
Size: 281 344 2350 415
Fragments arranged by size:
2350 415 344 281
NdeI CA'TA_TG
Cuts at: 0 2115 3390
Size: 2115 1275
NsiI A_TGCA'T
Cuts at: 0 2859 3390
Size: 2859 531
NspI r_CATG'y
Cuts at: 0 1265 3390
Size: 1265 2125
PflMI CCAn_nnn'nTGG
Cuts at: 0 445 2804 3390
Size: 445 2359 586
Fragments arranged by size:
2359 586 445
PfoI T'CCnGG_A
Cuts at: 0 299 3390
Size: 299 3091
PmlI CAC'GTG
Cuts at: 0 1506 3390
Size: 1506 1884
PpuMI rG'GwC_Cy
Cuts at: 0 116 201 3390
Size: 116 85 3189
Fragments arranged by size:
3189 116 85
PshAI GACnn'nnGTC
Cuts at: 0 362 3390
Size: 362 3028
PstI C_TGCA'G
Cuts at: 0 364 1078 3390
Size: 364 714 2312
Fragments arranged by size:
2312 714 364
PvuII CAG'CTG
Cuts at: 0 70 1743 3003 3390
Size: 70 1673 1260 387
Fragments arranged by size:
1673 1260 387 70
SacI G_AGCT'C
Cuts at: 0 34 3390
Size: 34 3356
SapI GCTCTTCn'nnn_
Cuts at: 0 998 3390
Size: 998 2392
ScaI AGT'ACT
Cuts at: 0 1487 1720 3390
Size: 1487 233 1670
Fragments arranged by size:
1670 1487 233
SexAI A'CCwGG_T
Cuts at: 0 500 3390
Size: 500 2890
SfcI C'TryA_G
Cuts at: 0 360 815 1074 1857 2217 2829 3390
Size: 360 455 259 783 360 612 561
Fragments arranged by size:
783 612 561 455 360 360 259
SfoI GGC'GCC
Cuts at: 0 41 133 3390
Size: 41 92 3257
Fragments arranged by size:
3257 92 41
SmaI CCC'GGG
Cuts at: 0 114 138 2084 3390
Size: 114 24 1946 1306
Fragments arranged by size:
1946 1306 114 24
SmlI C'TyrA_G
Cuts at: 0 1795 2166 3390
Size: 1795 371 1224
Fragments arranged by size:
1795 1224 371
StuI AGG'CCT
Cuts at: 0 1463 3390
Size: 1463 1927
StyI C'CwwG_G
Cuts at: 0 271 281 625 946 1285 2975 3390
Size: 271 10 344 321 339 1690 415
Fragments arranged by size:
1690 415 344 339 321 271 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 437 757 3390
Size: 437 320 2633
Fragments arranged by size:
2633 437 320
TatI w'GTAC_w
Cuts at: 0 408 1078 1485 1718 3390
Size: 408 670 407 233 1672
Fragments arranged by size:
1672 670 408 407 233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 546 578 3390
Size: 546 32 2812
Fragments arranged by size:
2812 546 32
Tth111I GACn'n_nGTC
Cuts at: 0 3058 3390
Size: 3058 332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 446 465 2062 3390
Size: 446 19 1597 1328
Fragments arranged by size:
1597 1328 446 19
XmaI C'CCGG_G
Cuts at: 0 112 136 2082 3390
Size: 112 24 1946 1308
Fragments arranged by size:
1946 1308 112 24
XmnI GAAnn'nnTTC
Cuts at: 0 3223 3390
Size: 3223 167
Enzymes that do cut:
AarI AccI AcuI AflII AhdI AleI AlfI AlwNI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrDI BssSI BstAPI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HindIII KasI MfeI MmeI MscI MslI
MspA1I NarI NcoI NdeI NsiI NspI PflMI PfoI
PmlI PpuMI PshAI PstI PvuII SacI SapI ScaI
SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AloI ApaI
ApaLI AscI AseI AsiSI BaeI BglI BlpI BmtI
BsaBI BsiEI BsiWI BspHI BsrBI BsrFI BsrGI BssHII
BstBI BstEII ClaI CspCI DrdI EagI EcoRV FseI
FspI FspAI HincII HpaI KpnI MluI NaeI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI ZraI