(Linear) (Six-base) MAPSORT of: KIEE3543.seq Check: 4069 from: 1 to: 3648 KIEE3543.orf With 182 enzymes: SgfI * July 1, 2016 17:02 .. AccI GT'mk_AC Cuts at: 0 375 2458 3648 Size: 375 2083 1190 Fragments arranged by size: 2083 1190 375 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 821 1023 1351 1612 2315 2665 2861 3648 Size: 821 202 328 261 703 350 196 787 Fragments arranged by size: 821 787 703 350 328 261 202 196 AflIII A'CryG_T Cuts at: 0 235 297 3648 Size: 235 62 3351 Fragments arranged by size: 3351 235 62 AhdI GACnn_n'nnGTC Cuts at: 0 2090 2742 3648 Size: 2090 652 906 Fragments arranged by size: 2090 906 652 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1106 3648 Size: 1106 2542 AlwNI CAG_nnn'CTG Cuts at: 0 11 718 1071 1203 1215 1329 1461 1678 Size: 11 707 353 132 12 114 132 217 Cuts at: 1678 2284 2302 3394 3648 Size: 606 18 1092 254 Fragments arranged by size: 1092 707 606 353 254 217 132 132 114 18 12 11 ApaI G_GGCC'C Cuts at: 0 124 141 2210 2887 3648 Size: 124 17 2069 677 761 Fragments arranged by size: 2069 761 677 124 17 ApoI r'AATT_y Cuts at: 0 606 2499 3648 Size: 606 1893 1149 Fragments arranged by size: 1893 1149 606 AvaI C'yCGr_G Cuts at: 0 115 134 3058 3648 Size: 115 19 2924 590 Fragments arranged by size: 2924 590 115 19 BanI G'GyrC_C Cuts at: 0 1059 1993 2439 3648 Size: 1059 934 446 1209 Fragments arranged by size: 1209 1059 934 446 BanII G_rGCy'C Cuts at: 0 124 141 1269 1486 1727 1744 2210 2517 Size: 124 17 1128 217 241 17 466 307 Cuts at: 2517 2887 3648 Size: 370 761 Fragments arranged by size: 1128 761 466 370 307 241 217 124 17 17 BbeI G_GCGC'C Cuts at: 0 2443 3648 Size: 2443 1205 BbvCI CC'TCA_GC Cuts at: 0 1619 3184 3648 Size: 1619 1565 464 Fragments arranged by size: 1619 1565 464 BclI T'GATC_A Cuts at: 0 2190 2661 2684 3648 Size: 2190 471 23 964 Fragments arranged by size: 2190 964 471 23 BfrBI ATG'CAT Cuts at: 0 1524 3648 Size: 1524 2124 BglI GCCn_nnn'nGGC Cuts at: 0 1490 2002 2758 2933 3648 Size: 1490 512 756 175 715 Fragments arranged by size: 1490 756 715 512 175 BglII A'GATC_T Cuts at: 0 2603 3437 3648 Size: 2603 834 211 Fragments arranged by size: 2603 834 211 BlpI GC'TnA_GC Cuts at: 0 893 1888 3004 3648 Size: 893 995 1116 644 Fragments arranged by size: 1116 995 893 644 Bme1580I G_kGCm'C Cuts at: 0 124 141 1064 1998 2111 2210 2833 2887 Size: 124 17 923 934 113 99 623 54 Cuts at: 2887 3648 Size: 761 Fragments arranged by size: 934 923 761 623 124 113 99 54 17 BmgBI CAC'GTC Cuts at: 0 2456 3648 Size: 2456 1192 BmrI ACTGGGnnnn_n' Cuts at: 0 627 973 1822 2312 3648 Size: 627 346 849 490 1336 Fragments arranged by size: 1336 849 627 490 346 BmtI G_CTAG'C Cuts at: 0 778 3648 Size: 778 2870 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2291 3648 Size: 2291 1357 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 137 715 869 920 1558 1561 1936 2261 Size: 137 578 154 51 638 3 375 325 Cuts at: 2261 2298 2392 2419 2611 2694 2791 3648 Size: 37 94 27 192 83 97 857 Fragments arranged by size: 857 638 578 375 325 192 154 137 97 94 83 51 37 27 3 Bpu10I CC'TnA_GC Cuts at: 0 1619 3124 3184 3648 Size: 1619 1505 60 464 Fragments arranged by size: 1619 1505 464 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 2584 3648 Size: 202 2382 1064 Fragments arranged by size: 2382 1064 202 BsaI GGTCTCn'nnnn_ Cuts at: 0 1082 1696 3648 Size: 1082 614 1952 Fragments arranged by size: 1952 1082 614 BsaHI Gr'CG_yC Cuts at: 0 1795 2440 3648 Size: 1795 645 1208 Fragments arranged by size: 1795 1208 645 BsaWI w'CCGG_w Cuts at: 0 3269 3648 Size: 3269 379 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1373 1403 1599 1629 1909 1939 2328 2358 Size: 1373 30 196 30 280 30 389 30 Cuts at: 2358 3011 3041 3439 3469 3499 3648 Size: 653 30 398 30 30 149 Fragments arranged by size: 1373 653 398 389 280 196 149 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 939 1607 1714 1842 2416 2607 3331 3505 Size: 939 668 107 128 574 191 724 174 Cuts at: 3505 3648 Size: 143 Fragments arranged by size: 939 724 668 574 191 174 143 128 107 BseYI C'CCAG_C Cuts at: 0 1015 1026 1064 1071 1166 1196 1203 1223 Size: 1015 11 38 7 95 30 7 20 Cuts at: 1223 1274 1454 1588 1728 2077 2110 2203 2209 Size: 51 180 134 140 349 33 93 6 Cuts at: 2209 2803 2889 3053 3294 3461 3648 Size: 594 86 164 241 167 187 Fragments arranged by size: 1015 594 349 241 187 180 167 164 140 134 95 93 86 51 38 33 30 20 11 7 7 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 96 312 622 3648 Size: 36 60 216 310 3026 Fragments arranged by size: 3026 310 216 60 36 BsiHKAI G_wGCw'C Cuts at: 0 949 1090 1192 2028 3648 Size: 949 141 102 836 1620 Fragments arranged by size: 1620 949 836 141 102 BsmI GAATG_Cn' Cuts at: 0 2382 3087 3110 3648 Size: 2382 705 23 538 Fragments arranged by size: 2382 705 538 23 Bsp1286I G_dGCh'C Cuts at: 0 124 141 949 1064 1090 1192 1269 1486 Size: 124 17 808 115 26 102 77 217 Cuts at: 1486 1727 1744 1998 2028 2111 2210 2517 2833 Size: 241 17 254 30 83 99 307 316 Cuts at: 2833 2887 3648 Size: 54 761 Fragments arranged by size: 808 761 316 307 254 241 217 124 115 102 99 83 77 54 30 26 17 17 BspEI T'CCGG_A Cuts at: 0 3269 3648 Size: 3269 379 BsrFI r'CCGG_y Cuts at: 0 1749 3648 Size: 1749 1899 BssSI C'ACGA_G Cuts at: 0 1477 3648 Size: 1477 2171 BstAPI GCAn_nnn'nTGC Cuts at: 0 59 83 690 3648 Size: 59 24 607 2958 Fragments arranged by size: 2958 607 59 24 BstXI CCAn_nnnn'nTGG Cuts at: 0 1798 1914 3648 Size: 1798 116 1734 Fragments arranged by size: 1798 1734 116 BstYI r'GATC_y Cuts at: 0 148 714 1964 2603 3437 3648 Size: 148 566 1250 639 834 211 Fragments arranged by size: 1250 834 639 566 211 148 Bsu36I CC'TnA_GG Cuts at: 0 2165 2198 2619 3648 Size: 2165 33 421 1029 Fragments arranged by size: 2165 1029 421 33 BtgI C'CryG_G Cuts at: 0 1175 3251 3535 3648 Size: 1175 2076 284 113 Fragments arranged by size: 2076 1175 284 113 BtsI GCAGTG_nn' Cuts at: 0 947 969 1090 3648 Size: 947 22 121 2558 Fragments arranged by size: 2558 947 121 22 EaeI y'GGCC_r Cuts at: 0 2 458 1314 2322 2812 3396 3626 3648 Size: 2 456 856 1008 490 584 230 22 Fragments arranged by size: 1008 856 584 490 456 230 22 2 EarI CTCTTCn'nnn_ Cuts at: 0 25 1448 3648 Size: 25 1423 2200 Fragments arranged by size: 2200 1423 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2951 3648 Size: 2951 697 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 137 715 821 869 920 1023 1351 1558 Size: 137 578 106 48 51 103 328 207 Cuts at: 1558 1561 1612 1936 2261 2298 2315 2392 2419 Size: 3 51 324 325 37 17 77 27 Cuts at: 2419 2611 2665 2694 2791 2861 3648 Size: 192 54 29 97 70 787 Fragments arranged by size: 787 578 328 325 324 207 192 137 106 103 97 77 70 54 51 51 48 37 29 27 17 3 EcoO109I rG'GnC_Cy Cuts at: 0 120 1005 1949 2760 2883 2884 3291 3648 Size: 120 885 944 811 123 1 407 357 Fragments arranged by size: 944 885 811 407 357 123 120 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 922 3648 Size: 922 2726 HaeII r_GCGC'y Cuts at: 0 2443 3648 Size: 2443 1205 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 706 738 1177 1209 1599 1631 1915 1947 Size: 706 32 439 32 390 32 284 32 Cuts at: 1947 2076 2108 2328 2360 2588 2620 2728 2760 Size: 129 32 220 32 228 32 108 32 Cuts at: 2760 2818 2850 3310 3342 3469 3481 3501 3513 Size: 58 32 460 32 127 12 20 12 Cuts at: 3513 3648 Size: 135 Fragments arranged by size: 706 460 439 390 284 228 220 135 129 127 108 58 32 32 32 32 32 32 32 32 32 32 20 12 12 KasI G'GCGC_C Cuts at: 0 2439 3648 Size: 2439 1209 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1922 1931 3588 3648 Size: 1922 9 1657 60 Fragments arranged by size: 1922 1657 60 9 MscI TGG'CCA Cuts at: 0 4 460 1316 3628 3648 Size: 4 456 856 2312 20 Fragments arranged by size: 2312 856 456 20 4 MslI CAynn'nnrTG Cuts at: 0 957 3648 Size: 957 2691 MspA1I CmG'CkG Cuts at: 0 1071 1200 1215 1990 2811 2960 3401 3648 Size: 1071 129 15 775 821 149 441 247 Fragments arranged by size: 1071 821 775 441 247 149 129 15 NaeI GCC'GGC Cuts at: 0 1751 3648 Size: 1751 1897 NarI GG'CG_CC Cuts at: 0 2440 3648 Size: 2440 1208 NcoI C'CATG_G Cuts at: 0 3251 3648 Size: 3251 397 NdeI CA'TA_TG Cuts at: 0 3587 3648 Size: 3587 61 NgoMIV G'CCGG_C Cuts at: 0 1749 3648 Size: 1749 1899 NheI G'CTAG_C Cuts at: 0 774 3648 Size: 774 2874 NsiI A_TGCA'T Cuts at: 0 1526 3648 Size: 1526 2122 NspI r_CATG'y Cuts at: 0 239 301 601 2292 2718 3132 3552 3648 Size: 239 62 300 1691 426 414 420 96 Fragments arranged by size: 1691 426 420 414 300 239 96 62 PasI CC'CwG_GG Cuts at: 0 1103 3013 3525 3648 Size: 1103 1910 512 123 Fragments arranged by size: 1910 1103 512 123 PciI A'CATG_T Cuts at: 0 235 297 3648 Size: 235 62 3351 Fragments arranged by size: 3351 235 62 PflMI CCAn_nnn'nTGG Cuts at: 0 1071 1203 1461 2302 3648 Size: 1071 132 258 841 1346 Fragments arranged by size: 1346 1071 841 258 132 PfoI T'CCnGG_A Cuts at: 0 153 171 3648 Size: 153 18 3477 Fragments arranged by size: 3477 153 18 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2837 2869 3498 3530 3648 Size: 2837 32 629 32 118 Fragments arranged by size: 2837 629 118 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1005 3291 3648 Size: 1005 2286 357 Fragments arranged by size: 2286 1005 357 PshAI GACnn'nnGTC Cuts at: 0 813 3648 Size: 813 2835 PspOMI G'GGCC_C Cuts at: 0 120 137 2206 2883 3648 Size: 120 17 2069 677 765 Fragments arranged by size: 2069 765 677 120 17 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 545 577 3648 Size: 545 32 3071 Fragments arranged by size: 3071 545 32 PstI C_TGCA'G Cuts at: 0 631 1085 1126 1151 1712 2267 2471 3648 Size: 631 454 41 25 561 555 204 1177 Fragments arranged by size: 1177 631 561 555 454 204 41 25 PvuII CAG'CTG Cuts at: 0 1071 1215 1990 3648 Size: 1071 144 775 1658 Fragments arranged by size: 1658 1071 775 144 SbfI CC_TGCA'GG Cuts at: 0 1712 3648 Size: 1712 1936 ScaI AGT'ACT Cuts at: 0 215 3648 Size: 215 3433 SexAI A'CCwGG_T Cuts at: 0 1936 3648 Size: 1936 1712 SfcI C'TryA_G Cuts at: 0 376 627 1081 1122 1147 1708 1715 2098 Size: 376 251 454 41 25 561 7 383 Cuts at: 2098 2263 2459 2467 3648 Size: 165 196 8 1181 Fragments arranged by size: 1181 561 454 383 376 251 196 165 41 25 8 7 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2758 3648 Size: 2758 890 SfoI GGC'GCC Cuts at: 0 2441 3648 Size: 2441 1207 SmaI CCC'GGG Cuts at: 0 117 3648 Size: 117 3531 SmlI C'TyrA_G Cuts at: 0 217 2599 3648 Size: 217 2382 1049 Fragments arranged by size: 2382 1049 217 StyI C'CwwG_G Cuts at: 0 727 3251 3466 3648 Size: 727 2524 215 182 Fragments arranged by size: 2524 727 215 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1029 1040 1451 2245 2770 3015 3648 Size: 1029 11 411 794 525 245 633 Fragments arranged by size: 1029 794 633 525 411 245 11 TatI w'GTAC_w Cuts at: 0 213 3648 Size: 213 3435 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 911 943 1283 1315 1373 1405 3011 3043 Size: 911 32 340 32 58 32 1606 32 Cuts at: 3043 3648 Size: 605 Fragments arranged by size: 1606 911 605 340 58 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1309 3020 3648 Size: 1309 1711 628 Fragments arranged by size: 1711 1309 628 XmaI C'CCGG_G Cuts at: 0 115 3648 Size: 115 3533 Enzymes that do cut: AccI AcuI AflIII AhdI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbvCI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PsrI PstI PvuII SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BsaAI BsaBI BsiEI BsiWI BsmBI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NotI NruI PacI PmeI PmlI PsiI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI