Restriction map for
pF1KE3543
(Linear) (Six-base) MAPSORT of: KIEE3543.seq Check: 4069 from: 1 to: 3648
KIEE3543.orf
With 182 enzymes: SgfI *
July 1, 2016 17:02 ..
AccI GT'mk_AC
Cuts at: 0 375 2458 3648
Size: 375 2083 1190
Fragments arranged by size:
2083 1190 375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1023 1351 1612 2315 2665 2861 3648
Size: 821 202 328 261 703 350 196 787
Fragments arranged by size:
821 787 703 350 328 261 202 196
AflIII A'CryG_T
Cuts at: 0 235 297 3648
Size: 235 62 3351
Fragments arranged by size:
3351 235 62
AhdI GACnn_n'nnGTC
Cuts at: 0 2090 2742 3648
Size: 2090 652 906
Fragments arranged by size:
2090 906 652
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1106 3648
Size: 1106 2542
AlwNI CAG_nnn'CTG
Cuts at: 0 11 718 1071 1203 1215 1329 1461 1678
Size: 11 707 353 132 12 114 132 217
Cuts at: 1678 2284 2302 3394 3648
Size: 606 18 1092 254
Fragments arranged by size:
1092 707 606 353 254 217 132 132
114 18 12 11
ApaI G_GGCC'C
Cuts at: 0 124 141 2210 2887 3648
Size: 124 17 2069 677 761
Fragments arranged by size:
2069 761 677 124 17
ApoI r'AATT_y
Cuts at: 0 606 2499 3648
Size: 606 1893 1149
Fragments arranged by size:
1893 1149 606
AvaI C'yCGr_G
Cuts at: 0 115 134 3058 3648
Size: 115 19 2924 590
Fragments arranged by size:
2924 590 115 19
BanI G'GyrC_C
Cuts at: 0 1059 1993 2439 3648
Size: 1059 934 446 1209
Fragments arranged by size:
1209 1059 934 446
BanII G_rGCy'C
Cuts at: 0 124 141 1269 1486 1727 1744 2210 2517
Size: 124 17 1128 217 241 17 466 307
Cuts at: 2517 2887 3648
Size: 370 761
Fragments arranged by size:
1128 761 466 370 307 241 217 124
17 17
BbeI G_GCGC'C
Cuts at: 0 2443 3648
Size: 2443 1205
BbvCI CC'TCA_GC
Cuts at: 0 1619 3184 3648
Size: 1619 1565 464
Fragments arranged by size:
1619 1565 464
BclI T'GATC_A
Cuts at: 0 2190 2661 2684 3648
Size: 2190 471 23 964
Fragments arranged by size:
2190 964 471 23
BfrBI ATG'CAT
Cuts at: 0 1524 3648
Size: 1524 2124
BglI GCCn_nnn'nGGC
Cuts at: 0 1490 2002 2758 2933 3648
Size: 1490 512 756 175 715
Fragments arranged by size:
1490 756 715 512 175
BglII A'GATC_T
Cuts at: 0 2603 3437 3648
Size: 2603 834 211
Fragments arranged by size:
2603 834 211
BlpI GC'TnA_GC
Cuts at: 0 893 1888 3004 3648
Size: 893 995 1116 644
Fragments arranged by size:
1116 995 893 644
Bme1580I G_kGCm'C
Cuts at: 0 124 141 1064 1998 2111 2210 2833 2887
Size: 124 17 923 934 113 99 623 54
Cuts at: 2887 3648
Size: 761
Fragments arranged by size:
934 923 761 623 124 113 99 54
17
BmgBI CAC'GTC
Cuts at: 0 2456 3648
Size: 2456 1192
BmrI ACTGGGnnnn_n'
Cuts at: 0 627 973 1822 2312 3648
Size: 627 346 849 490 1336
Fragments arranged by size:
1336 849 627 490 346
BmtI G_CTAG'C
Cuts at: 0 778 3648
Size: 778 2870
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2291 3648
Size: 2291 1357
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 715 869 920 1558 1561 1936 2261
Size: 137 578 154 51 638 3 375 325
Cuts at: 2261 2298 2392 2419 2611 2694 2791 3648
Size: 37 94 27 192 83 97 857
Fragments arranged by size:
857 638 578 375 325 192 154 137
97 94 83 51 37 27 3
Bpu10I CC'TnA_GC
Cuts at: 0 1619 3124 3184 3648
Size: 1619 1505 60 464
Fragments arranged by size:
1619 1505 464 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 2584 3648
Size: 202 2382 1064
Fragments arranged by size:
2382 1064 202
BsaI GGTCTCn'nnnn_
Cuts at: 0 1082 1696 3648
Size: 1082 614 1952
Fragments arranged by size:
1952 1082 614
BsaHI Gr'CG_yC
Cuts at: 0 1795 2440 3648
Size: 1795 645 1208
Fragments arranged by size:
1795 1208 645
BsaWI w'CCGG_w
Cuts at: 0 3269 3648
Size: 3269 379
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1373 1403 1599 1629 1909 1939 2328 2358
Size: 1373 30 196 30 280 30 389 30
Cuts at: 2358 3011 3041 3439 3469 3499 3648
Size: 653 30 398 30 30 149
Fragments arranged by size:
1373 653 398 389 280 196 149 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 939 1607 1714 1842 2416 2607 3331 3505
Size: 939 668 107 128 574 191 724 174
Cuts at: 3505 3648
Size: 143
Fragments arranged by size:
939 724 668 574 191 174 143 128
107
BseYI C'CCAG_C
Cuts at: 0 1015 1026 1064 1071 1166 1196 1203 1223
Size: 1015 11 38 7 95 30 7 20
Cuts at: 1223 1274 1454 1588 1728 2077 2110 2203 2209
Size: 51 180 134 140 349 33 93 6
Cuts at: 2209 2803 2889 3053 3294 3461 3648
Size: 594 86 164 241 167 187
Fragments arranged by size:
1015 594 349 241 187 180 167 164
140 134 95 93 86 51 38 33
30 20 11 7 7 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 96 312 622 3648
Size: 36 60 216 310 3026
Fragments arranged by size:
3026 310 216 60 36
BsiHKAI G_wGCw'C
Cuts at: 0 949 1090 1192 2028 3648
Size: 949 141 102 836 1620
Fragments arranged by size:
1620 949 836 141 102
BsmI GAATG_Cn'
Cuts at: 0 2382 3087 3110 3648
Size: 2382 705 23 538
Fragments arranged by size:
2382 705 538 23
Bsp1286I G_dGCh'C
Cuts at: 0 124 141 949 1064 1090 1192 1269 1486
Size: 124 17 808 115 26 102 77 217
Cuts at: 1486 1727 1744 1998 2028 2111 2210 2517 2833
Size: 241 17 254 30 83 99 307 316
Cuts at: 2833 2887 3648
Size: 54 761
Fragments arranged by size:
808 761 316 307 254 241 217 124
115 102 99 83 77 54 30 26
17 17
BspEI T'CCGG_A
Cuts at: 0 3269 3648
Size: 3269 379
BsrFI r'CCGG_y
Cuts at: 0 1749 3648
Size: 1749 1899
BssSI C'ACGA_G
Cuts at: 0 1477 3648
Size: 1477 2171
BstAPI GCAn_nnn'nTGC
Cuts at: 0 59 83 690 3648
Size: 59 24 607 2958
Fragments arranged by size:
2958 607 59 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1798 1914 3648
Size: 1798 116 1734
Fragments arranged by size:
1798 1734 116
BstYI r'GATC_y
Cuts at: 0 148 714 1964 2603 3437 3648
Size: 148 566 1250 639 834 211
Fragments arranged by size:
1250 834 639 566 211 148
Bsu36I CC'TnA_GG
Cuts at: 0 2165 2198 2619 3648
Size: 2165 33 421 1029
Fragments arranged by size:
2165 1029 421 33
BtgI C'CryG_G
Cuts at: 0 1175 3251 3535 3648
Size: 1175 2076 284 113
Fragments arranged by size:
2076 1175 284 113
BtsI GCAGTG_nn'
Cuts at: 0 947 969 1090 3648
Size: 947 22 121 2558
Fragments arranged by size:
2558 947 121 22
EaeI y'GGCC_r
Cuts at: 0 2 458 1314 2322 2812 3396 3626 3648
Size: 2 456 856 1008 490 584 230 22
Fragments arranged by size:
1008 856 584 490 456 230 22 2
EarI CTCTTCn'nnn_
Cuts at: 0 25 1448 3648
Size: 25 1423 2200
Fragments arranged by size:
2200 1423 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2951 3648
Size: 2951 697
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 715 821 869 920 1023 1351 1558
Size: 137 578 106 48 51 103 328 207
Cuts at: 1558 1561 1612 1936 2261 2298 2315 2392 2419
Size: 3 51 324 325 37 17 77 27
Cuts at: 2419 2611 2665 2694 2791 2861 3648
Size: 192 54 29 97 70 787
Fragments arranged by size:
787 578 328 325 324 207 192 137
106 103 97 77 70 54 51 51
48 37 29 27 17 3
EcoO109I rG'GnC_Cy
Cuts at: 0 120 1005 1949 2760 2883 2884 3291 3648
Size: 120 885 944 811 123 1 407 357
Fragments arranged by size:
944 885 811 407 357 123 120 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 922 3648
Size: 922 2726
HaeII r_GCGC'y
Cuts at: 0 2443 3648
Size: 2443 1205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 706 738 1177 1209 1599 1631 1915 1947
Size: 706 32 439 32 390 32 284 32
Cuts at: 1947 2076 2108 2328 2360 2588 2620 2728 2760
Size: 129 32 220 32 228 32 108 32
Cuts at: 2760 2818 2850 3310 3342 3469 3481 3501 3513
Size: 58 32 460 32 127 12 20 12
Cuts at: 3513 3648
Size: 135
Fragments arranged by size:
706 460 439 390 284 228 220 135
129 127 108 58 32 32 32 32
32 32 32 32 32 32 20 12
12
KasI G'GCGC_C
Cuts at: 0 2439 3648
Size: 2439 1209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1922 1931 3588 3648
Size: 1922 9 1657 60
Fragments arranged by size:
1922 1657 60 9
MscI TGG'CCA
Cuts at: 0 4 460 1316 3628 3648
Size: 4 456 856 2312 20
Fragments arranged by size:
2312 856 456 20 4
MslI CAynn'nnrTG
Cuts at: 0 957 3648
Size: 957 2691
MspA1I CmG'CkG
Cuts at: 0 1071 1200 1215 1990 2811 2960 3401 3648
Size: 1071 129 15 775 821 149 441 247
Fragments arranged by size:
1071 821 775 441 247 149 129 15
NaeI GCC'GGC
Cuts at: 0 1751 3648
Size: 1751 1897
NarI GG'CG_CC
Cuts at: 0 2440 3648
Size: 2440 1208
NcoI C'CATG_G
Cuts at: 0 3251 3648
Size: 3251 397
NdeI CA'TA_TG
Cuts at: 0 3587 3648
Size: 3587 61
NgoMIV G'CCGG_C
Cuts at: 0 1749 3648
Size: 1749 1899
NheI G'CTAG_C
Cuts at: 0 774 3648
Size: 774 2874
NsiI A_TGCA'T
Cuts at: 0 1526 3648
Size: 1526 2122
NspI r_CATG'y
Cuts at: 0 239 301 601 2292 2718 3132 3552 3648
Size: 239 62 300 1691 426 414 420 96
Fragments arranged by size:
1691 426 420 414 300 239 96 62
PasI CC'CwG_GG
Cuts at: 0 1103 3013 3525 3648
Size: 1103 1910 512 123
Fragments arranged by size:
1910 1103 512 123
PciI A'CATG_T
Cuts at: 0 235 297 3648
Size: 235 62 3351
Fragments arranged by size:
3351 235 62
PflMI CCAn_nnn'nTGG
Cuts at: 0 1071 1203 1461 2302 3648
Size: 1071 132 258 841 1346
Fragments arranged by size:
1346 1071 841 258 132
PfoI T'CCnGG_A
Cuts at: 0 153 171 3648
Size: 153 18 3477
Fragments arranged by size:
3477 153 18
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2837 2869 3498 3530 3648
Size: 2837 32 629 32 118
Fragments arranged by size:
2837 629 118 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1005 3291 3648
Size: 1005 2286 357
Fragments arranged by size:
2286 1005 357
PshAI GACnn'nnGTC
Cuts at: 0 813 3648
Size: 813 2835
PspOMI G'GGCC_C
Cuts at: 0 120 137 2206 2883 3648
Size: 120 17 2069 677 765
Fragments arranged by size:
2069 765 677 120 17
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 545 577 3648
Size: 545 32 3071
Fragments arranged by size:
3071 545 32
PstI C_TGCA'G
Cuts at: 0 631 1085 1126 1151 1712 2267 2471 3648
Size: 631 454 41 25 561 555 204 1177
Fragments arranged by size:
1177 631 561 555 454 204 41 25
PvuII CAG'CTG
Cuts at: 0 1071 1215 1990 3648
Size: 1071 144 775 1658
Fragments arranged by size:
1658 1071 775 144
SbfI CC_TGCA'GG
Cuts at: 0 1712 3648
Size: 1712 1936
ScaI AGT'ACT
Cuts at: 0 215 3648
Size: 215 3433
SexAI A'CCwGG_T
Cuts at: 0 1936 3648
Size: 1936 1712
SfcI C'TryA_G
Cuts at: 0 376 627 1081 1122 1147 1708 1715 2098
Size: 376 251 454 41 25 561 7 383
Cuts at: 2098 2263 2459 2467 3648
Size: 165 196 8 1181
Fragments arranged by size:
1181 561 454 383 376 251 196 165
41 25 8 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2758 3648
Size: 2758 890
SfoI GGC'GCC
Cuts at: 0 2441 3648
Size: 2441 1207
SmaI CCC'GGG
Cuts at: 0 117 3648
Size: 117 3531
SmlI C'TyrA_G
Cuts at: 0 217 2599 3648
Size: 217 2382 1049
Fragments arranged by size:
2382 1049 217
StyI C'CwwG_G
Cuts at: 0 727 3251 3466 3648
Size: 727 2524 215 182
Fragments arranged by size:
2524 727 215 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1029 1040 1451 2245 2770 3015 3648
Size: 1029 11 411 794 525 245 633
Fragments arranged by size:
1029 794 633 525 411 245 11
TatI w'GTAC_w
Cuts at: 0 213 3648
Size: 213 3435
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 911 943 1283 1315 1373 1405 3011 3043
Size: 911 32 340 32 58 32 1606 32
Cuts at: 3043 3648
Size: 605
Fragments arranged by size:
1606 911 605 340 58 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1309 3020 3648
Size: 1309 1711 628
Fragments arranged by size:
1711 1309 628
XmaI C'CCGG_G
Cuts at: 0 115 3648
Size: 115 3533
Enzymes that do cut:
AccI AcuI AflIII AhdI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbvCI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BsrFI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtsI EaeI EarI EciI
Eco57MI EcoO109I FalI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuII SbfI ScaI SexAI
SfcI SfiI SfoI SmaI SmlI StyI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BcgI BciVI BsaAI BsaBI BsiEI BsiWI BsmBI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NotI NruI PacI
PmeI PmlI PsiI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI
ZraI