Restriction map for
pF1KE3561
(Linear) (Six-base) MAPSORT of: KIEE3561.seq Check: 43 from: 1 to: 2778
KIEE3561.orf
With 182 enzymes: SgfI *
August 31, 2012 11:48 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 805 2113 2677 2778
Size: 716 89 1308 564 101
Fragments arranged by size:
1308 716 564 101 89
AflII C'TTAA_G
Cuts at: 0 1543 2778
Size: 1543 1235
AflIII A'CryG_T
Cuts at: 0 707 1972 2778
Size: 707 1265 806
Fragments arranged by size:
1265 806 707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1100 2733 2778
Size: 1100 1633 45
Fragments arranged by size:
1633 1100 45
ApaI G_GGCC'C
Cuts at: 0 1716 2778
Size: 1716 1062
ApoI r'AATT_y
Cuts at: 0 355 477 536 1377 1550 2048 2086 2344
Size: 355 122 59 841 173 498 38 258
Cuts at: 2344 2778
Size: 434
Fragments arranged by size:
841 498 434 355 258 173 122 59
38
AseI AT'TA_AT
Cuts at: 0 2455 2778
Size: 2455 323
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2723 2756 2778
Size: 2723 33 22
Fragments arranged by size:
2723 33 22
BamHI G'GATC_C
Cuts at: 0 2239 2778
Size: 2239 539
BanI G'GyrC_C
Cuts at: 0 2257 2778
Size: 2257 521
BanII G_rGCy'C
Cuts at: 0 1716 2778
Size: 1716 1062
BbsI GAAGACnn'nnnn_
Cuts at: 0 161 1491 2778
Size: 161 1330 1287
Fragments arranged by size:
1330 1287 161
BclI T'GATC_A
Cuts at: 0 248 2778
Size: 248 2530
BfrBI ATG'CAT
Cuts at: 0 1077 1124 2778
Size: 1077 47 1654
Fragments arranged by size:
1654 1077 47
BglI GCCn_nnn'nGGC
Cuts at: 0 1159 2284 2778
Size: 1159 1125 494
Fragments arranged by size:
1159 1125 494
BglII A'GATC_T
Cuts at: 0 2688 2778
Size: 2688 90
BlpI GC'TnA_GC
Cuts at: 0 1220 2778
Size: 1220 1558
Bme1580I G_kGCm'C
Cuts at: 0 569 1716 2778
Size: 569 1147 1062
Fragments arranged by size:
1147 1062 569
BmrI ACTGGGnnnn_n'
Cuts at: 0 292 563 1034 1719 1816 2778
Size: 292 271 471 685 97 962
Fragments arranged by size:
962 685 471 292 271 97
BmtI G_CTAG'C
Cuts at: 0 2059 2778
Size: 2059 719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2027 2778
Size: 2027 751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 351 450 2245 2778
Size: 293 58 99 1795 533
Fragments arranged by size:
1795 533 293 99 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2533 2778
Size: 2533 245
BsaBI GATnn'nnATC
Cuts at: 0 634 2778
Size: 634 2144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 373 403 2778
Size: 373 30 2375
Fragments arranged by size:
2375 373 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2248 2778
Size: 2248 530
BseYI C'CCAG_C
Cuts at: 0 2265 2647 2778
Size: 2265 382 131
Fragments arranged by size:
2265 382 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 2778
Size: 1012 1766
BsiHKAI G_wGCw'C
Cuts at: 0 1433 1729 2778
Size: 1433 296 1049
Fragments arranged by size:
1433 1049 296
BsmI GAATG_Cn'
Cuts at: 0 4 1579 2778
Size: 4 1575 1199
Fragments arranged by size:
1575 1199 4
Bsp1286I G_dGCh'C
Cuts at: 0 569 1433 1716 1729 2778
Size: 569 864 283 13 1049
Fragments arranged by size:
1049 864 569 283 13
BspHI T'CATG_A
Cuts at: 0 132 1190 2778
Size: 132 1058 1588
Fragments arranged by size:
1588 1058 132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 34 2778
Size: 34 2744
BsrBI CCG'CTC
Cuts at: 0 2624 2778
Size: 2624 154
BsrDI GCAATG_nn'
Cuts at: 0 332 599 2273 2588 2634 2778
Size: 332 267 1674 315 46 144
Fragments arranged by size:
1674 332 315 267 144 46
BsrFI r'CCGG_y
Cuts at: 0 2430 2778
Size: 2430 348
BsrGI T'GTAC_A
Cuts at: 0 307 2778
Size: 307 2471
BssSI C'ACGA_G
Cuts at: 0 1295 1938 2778
Size: 1295 643 840
Fragments arranged by size:
1295 840 643
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1258 1733 2024 2778
Size: 1258 475 291 754
Fragments arranged by size:
1258 754 475 291
BstYI r'GATC_y
Cuts at: 0 1281 2239 2688 2778
Size: 1281 958 449 90
Fragments arranged by size:
1281 958 449 90
BtgI C'CryG_G
Cuts at: 0 1327 2778
Size: 1327 1451
BtsI GCAGTG_nn'
Cuts at: 0 1259 1680 2778
Size: 1259 421 1098
Fragments arranged by size:
1259 1098 421
DraI TTT'AAA
Cuts at: 0 1409 2778
Size: 1409 1369
DraIII CAC_nnn'GTG
Cuts at: 0 1733 2778
Size: 1733 1045
EaeI y'GGCC_r
Cuts at: 0 1778 2778
Size: 1778 1000
EarI CTCTTCn'nnn_
Cuts at: 0 862 981 2778
Size: 862 119 1797
Fragments arranged by size:
1797 862 119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 351 450 716 805 2113 2245 2677
Size: 293 58 99 266 89 1308 132 432
Cuts at: 2677 2778
Size: 101
Fragments arranged by size:
1308 432 293 266 132 101 99 89
58
EcoO109I rG'GnC_Cy
Cuts at: 0 1713 2778
Size: 1713 1065
EcoRI G'AATT_C
Cuts at: 0 1377 2778
Size: 1377 1401
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 748 2559 2778
Size: 748 1811 219
Fragments arranged by size:
1811 748 219
FspI TGC'GCA
Cuts at: 0 1092 2778
Size: 1092 1686
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 161 193 898 930 1877 1909 2025 2057
Size: 161 32 705 32 947 32 116 32
Cuts at: 2057 2204 2224 2236 2256 2778
Size: 147 20 12 20 522
Fragments arranged by size:
947 705 522 161 147 116 32 32
32 32 20 20 12
HincII GTy'rAC
Cuts at: 0 917 1612 2778
Size: 917 695 1166
Fragments arranged by size:
1166 917 695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 2778
Size: 601 2177
MscI TGG'CCA
Cuts at: 0 1780 2778
Size: 1780 998
MslI CAynn'nnrTG
Cuts at: 0 244 874 1067 1391 1468 2715 2778
Size: 244 630 193 324 77 1247 63
Fragments arranged by size:
1247 630 324 244 193 77 63
NcoI C'CATG_G
Cuts at: 0 1327 2778
Size: 1327 1451
NdeI CA'TA_TG
Cuts at: 0 1095 2716 2778
Size: 1095 1621 62
Fragments arranged by size:
1621 1095 62
NheI G'CTAG_C
Cuts at: 0 2055 2778
Size: 2055 723
NsiI A_TGCA'T
Cuts at: 0 1079 1126 2778
Size: 1079 47 1652
Fragments arranged by size:
1652 1079 47
NspI r_CATG'y
Cuts at: 0 411 1976 2364 2778
Size: 411 1565 388 414
Fragments arranged by size:
1565 414 411 388
PciI A'CATG_T
Cuts at: 0 1972 2778
Size: 1972 806
PfoI T'CCnGG_A
Cuts at: 0 96 179 466 2222 2778
Size: 96 83 287 1756 556
Fragments arranged by size:
1756 556 287 96 83
PsiI TTA'TAA
Cuts at: 0 685 2778
Size: 685 2093
PspOMI G'GGCC_C
Cuts at: 0 1712 2778
Size: 1712 1066
PstI C_TGCA'G
Cuts at: 0 519 2778
Size: 519 2259
SfcI C'TryA_G
Cuts at: 0 515 1172 1486 2103 2186 2559 2778
Size: 515 657 314 617 83 373 219
Fragments arranged by size:
657 617 515 373 314 219 83
SmlI C'TyrA_G
Cuts at: 0 1543 2548 2778
Size: 1543 1005 230
Fragments arranged by size:
1543 1005 230
SphI G_CATG'C
Cuts at: 0 2364 2778
Size: 2364 414
SspI AAT'ATT
Cuts at: 0 1841 2778
Size: 1841 937
StyI C'CwwG_G
Cuts at: 0 1263 1327 1568 1775 2778
Size: 1263 64 241 207 1003
Fragments arranged by size:
1263 1003 241 207 64
SwaI ATTT'AAAT
Cuts at: 0 1409 2778
Size: 1409 1369
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2124 2778
Size: 2124 654
TatI w'GTAC_w
Cuts at: 0 307 2778
Size: 307 2471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 373 405 888 920 2778
Size: 373 32 483 32 1858
Fragments arranged by size:
1858 483 373 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2037 2778
Size: 2037 741
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 187 375 2778
Size: 187 188 2403
Fragments arranged by size:
2403 188 187
Enzymes that do cut:
AcuI AflII AflIII AlfI ApaI ApoI AseI BaeI
BamHI BanI BanII BbsI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaBI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstYI
BtgI BtsI DraI DraIII EaeI EarI Eco57MI EcoO109I
EcoRI FalI FspI Hin4I HincII MmeI MscI MslI
NcoI NdeI NheI NsiI NspI PciI PfoI PsiI
PspOMI PstI SfcI SmlI SphI SspI StyI SwaI
TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AloI AlwNI ApaLI AscI AsiSI AvaI AvrII
BbeI BbvCI BcgI BciVI BmgBI Bpu10I BsaI BsaAI
BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DrdI
EagI EciI EcoICRI EcoNI EcoRV FseI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI
NarI NgoMIV NotI NruI PacI PasI PflMI PmeI
PmlI PpiI PpuMI PshAI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI XbaI XhoI XmaI XmnI ZraI