Restriction map for
pF1KE3703
(Linear) (Six-base) MAPSORT of: KIEE3703.seq Check: 6921 from: 1 to: 5037
KIEE3703.orf
With 182 enzymes: SgfI *
May 29, 2012 11:57 ..
AatII G_ACGT'C
Cuts at: 0 3416 5037
Size: 3416 1621
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1839 2185 3280 3319 5037
Size: 1839 346 1095 39 1718
Fragments arranged by size:
1839 1718 1095 346 39
AfeI AGC'GCT
Cuts at: 0 4257 5037
Size: 4257 780
AflIII A'CryG_T
Cuts at: 0 4538 4778 5037
Size: 4538 240 259
Fragments arranged by size:
4538 259 240
AleI CACnn'nnGTG
Cuts at: 0 365 3733 4897 5037
Size: 365 3368 1164 140
Fragments arranged by size:
3368 1164 365 140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 869 3751 5037
Size: 869 2882 1286
Fragments arranged by size:
2882 1286 869
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4501 4533 5037
Size: 4501 32 504
Fragments arranged by size:
4501 504 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3157 3193 3401 4029 5037
Size: 3157 36 208 628 1008
Fragments arranged by size:
3157 1008 628 208 36
ApaI G_GGCC'C
Cuts at: 0 30 434 865 1310 2483 2621 2628 2665
Size: 30 404 431 445 1173 138 7 37
Cuts at: 2665 3287 4451 4908 5037
Size: 622 1164 457 129
Fragments arranged by size:
1173 1164 622 457 445 431 404 138
129 37 30 7
ApaLI G'TGCA_C
Cuts at: 0 4228 4396 5037
Size: 4228 168 641
Fragments arranged by size:
4228 641 168
ApoI r'AATT_y
Cuts at: 0 2050 5037
Size: 2050 2987
AseI AT'TA_AT
Cuts at: 0 4382 5037
Size: 4382 655
AvaI C'yCGr_G
Cuts at: 0 1472 1903 1963 1985 2627 2658 3212 3521
Size: 1472 431 60 22 642 31 554 309
Cuts at: 3521 5037
Size: 1516
Fragments arranged by size:
1516 1472 642 554 431 309 60 31
22
BanI G'GyrC_C
Cuts at: 0 206 237 1174 1859 3976 4134 4811 5037
Size: 206 31 937 685 2117 158 677 226
Fragments arranged by size:
2117 937 685 677 226 206 158 31
BanII G_rGCy'C
Cuts at: 0 30 88 434 865 1310 1347 1502 1873
Size: 30 58 346 431 445 37 155 371
Cuts at: 1873 2174 2483 2569 2621 2628 2665 3287 3652
Size: 301 309 86 52 7 37 622 365
Cuts at: 3652 3802 4451 4908 4972 4994 5037
Size: 150 649 457 64 22 43
Fragments arranged by size:
649 622 457 445 431 371 365 346
309 301 155 150 86 64 58 52
43 37 37 30 22 7
BbeI G_GCGC'C
Cuts at: 0 241 1863 4815 5037
Size: 241 1622 2952 222
Fragments arranged by size:
2952 1622 241 222
BbvCI CC'TCA_GC
Cuts at: 0 667 2544 5037
Size: 667 1877 2493
Fragments arranged by size:
2493 1877 667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3966 4000 4786 4820 5037
Size: 3966 34 786 34 217
Fragments arranged by size:
3966 786 217 34 34
BclI T'GATC_A
Cuts at: 0 4595 5037
Size: 4595 442
BfrBI ATG'CAT
Cuts at: 0 3 4327 5037
Size: 3 4324 710
Fragments arranged by size:
4324 710 3
BglI GCCn_nnn'nGGC
Cuts at: 0 677 1172 1465 3118 3647 4088 4832 4912
Size: 677 495 293 1653 529 441 744 80
Cuts at: 4912 5037
Size: 125
Fragments arranged by size:
1653 744 677 529 495 441 293 125
80
BglII A'GATC_T
Cuts at: 0 1487 3782 5037
Size: 1487 2295 1255
Fragments arranged by size:
2295 1487 1255
BlpI GC'TnA_GC
Cuts at: 0 141 422 4001 4078 5037
Size: 141 281 3579 77 959
Fragments arranged by size:
3579 959 281 141 77
Bme1580I G_kGCm'C
Cuts at: 0 30 209 434 865 1310 1600 2483 2621
Size: 30 179 225 431 445 290 883 138
Cuts at: 2621 2628 2665 3287 4139 4232 4400 4451 4529
Size: 7 37 622 852 93 168 51 78
Cuts at: 4529 4809 4908 4938 5037
Size: 280 99 30 99
Fragments arranged by size:
883 852 622 445 431 290 280 225
179 168 138 99 99 93 78 51
37 30 30 7
BmgBI CAC'GTC
Cuts at: 0 5030 5037
Size: 5030 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 604 2797 3474 3768 4251 4410 4948 5037
Size: 604 2193 677 294 483 159 538 89
Fragments arranged by size:
2193 677 604 538 483 294 159 89
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 102 149 215 538 695 926 1089
Size: 5 97 47 66 323 157 231 163
Cuts at: 1089 1300 1796 2044 2443 2474 3122 3580 4867
Size: 211 496 248 399 31 648 458 1287
Cuts at: 4867 5037
Size: 170
Fragments arranged by size:
1287 648 496 458 399 323 248 231
211 170 163 157 97 66 47 31
5
Bpu10I CC'TnA_GC
Cuts at: 0 64 667 2544 4874 4988 5037
Size: 64 603 1877 2330 114 49
Fragments arranged by size:
2330 1877 603 114 64 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 5037
Size: 565 4472
BsaI GGTCTCn'nnnn_
Cuts at: 0 1222 1290 1890 2749 3331 4240 4361 5037
Size: 1222 68 600 859 582 909 121 676
Fragments arranged by size:
1222 909 859 676 600 582 121 68
BsaAI yAC'GTr
Cuts at: 0 4086 4539 4781 5037
Size: 4086 453 242 256
Fragments arranged by size:
4086 453 256 242
BsaHI Gr'CG_yC
Cuts at: 0 238 1860 3413 4812 4886 5037
Size: 238 1622 1553 1399 74 151
Fragments arranged by size:
1622 1553 1399 238 151 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 511 541 1529 1559 2017 2047 2211 2241
Size: 511 30 988 30 458 30 164 30
Cuts at: 2241 2447 2477 4165 4195 4358 4388 5037
Size: 206 30 1688 30 163 30 649
Fragments arranged by size:
1688 988 649 511 458 206 164 163
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 98 385 673 2053 2305 2884 3082 3523
Size: 98 287 288 1380 252 579 198 441
Cuts at: 3523 3886 3907 3916 4879 4944 5037
Size: 363 21 9 963 65 93
Fragments arranged by size:
1380 963 579 441 363 288 287 252
198 98 93 65 21 9
BseYI C'CCAG_C
Cuts at: 0 73 211 314 426 458 936 1687 1753
Size: 73 138 103 112 32 478 751 66
Cuts at: 1753 1758 1949 2383 2793 3042 3170 3547 4016
Size: 5 191 434 410 249 128 377 469
Cuts at: 4016 5019 5037
Size: 1003 18
Fragments arranged by size:
1003 751 478 469 434 410 377 249
191 138 128 112 103 73 66 32
18 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 310 637 3143 3757 4138 4375 4426
Size: 20 290 327 2506 614 381 237 51
Cuts at: 4426 5037
Size: 611
Fragments arranged by size:
2506 614 611 381 327 290 237 51
20
BsiEI CG_ry'CG
Cuts at: 0 4976 5037
Size: 4976 61
BsiHKAI G_wGCw'C
Cuts at: 0 88 362 551 1502 3802 4232 4238 4400
Size: 88 274 189 951 2300 430 6 162
Cuts at: 4400 5037
Size: 637
Fragments arranged by size:
2300 951 637 430 274 189 162 88
6
BsmI GAATG_Cn'
Cuts at: 0 1244 3438 5037
Size: 1244 2194 1599
Fragments arranged by size:
2194 1599 1244
Bsp1286I G_dGCh'C
Cuts at: 0 30 88 209 362 434 551 865 1310
Size: 30 58 121 153 72 117 314 445
Cuts at: 1310 1347 1502 1600 1873 2174 2483 2569 2621
Size: 37 155 98 273 301 309 86 52
Cuts at: 2621 2628 2665 3287 3652 3802 4139 4232 4238
Size: 7 37 622 365 150 337 93 6
Cuts at: 4238 4400 4451 4529 4809 4908 4938 4972 4994
Size: 162 51 78 280 99 30 34 22
Cuts at: 4994 5037
Size: 43
Fragments arranged by size:
622 445 365 337 314 309 301 280
273 162 155 153 150 121 117 99
98 93 86 78 72 58 52 51
43 37 37 34 30 30 22 7
6
BspHI T'CATG_A
Cuts at: 0 2084 5037
Size: 2084 2953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3065 5037
Size: 3065 1972
BsrBI CCG'CTC
Cuts at: 0 2769 3366 5037
Size: 2769 597 1671
Fragments arranged by size:
2769 1671 597
BsrDI GCAATG_nn'
Cuts at: 0 295 3818 4430 5037
Size: 295 3523 612 607
Fragments arranged by size:
3523 612 607 295
BsrFI r'CCGG_y
Cuts at: 0 971 4414 4998 5037
Size: 971 3443 584 39
Fragments arranged by size:
3443 971 584 39
BsrGI T'GTAC_A
Cuts at: 0 3604 4124 5037
Size: 3604 520 913
Fragments arranged by size:
3604 913 520
BssHII G'CGCG_C
Cuts at: 0 3420 4915 5037
Size: 3420 1495 122
Fragments arranged by size:
3420 1495 122
BssSI C'ACGA_G
Cuts at: 0 4231 4372 5037
Size: 4231 141 665
Fragments arranged by size:
4231 665 141
BstAPI GCAn_nnn'nTGC
Cuts at: 0 297 624 915 3166 4029 4404 4721 5037
Size: 297 327 291 2251 863 375 317 316
Fragments arranged by size:
2251 863 375 327 317 316 297 291
BstXI CCAn_nnnn'nTGG
Cuts at: 0 326 833 2022 3690 5037
Size: 326 507 1189 1668 1347
Fragments arranged by size:
1668 1347 1189 507 326
BstYI r'GATC_y
Cuts at: 0 1487 2057 3071 3302 3762 3782 5037
Size: 1487 570 1014 231 460 20 1255
Fragments arranged by size:
1487 1255 1014 570 460 231 20
Bsu36I CC'TnA_GG
Cuts at: 0 651 1845 2610 5037
Size: 651 1194 765 2427
Fragments arranged by size:
2427 1194 765 651
BtgI C'CryG_G
Cuts at: 0 258 1029 1293 2273 3716 4064 5037
Size: 258 771 264 980 1443 348 973
Fragments arranged by size:
1443 980 973 771 348 264 258
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1537 4739 5037
Size: 1537 3202 298
Fragments arranged by size:
3202 1537 298
BtsI GCAGTG_nn'
Cuts at: 0 17 733 2897 4626 4791 5037
Size: 17 716 2164 1729 165 246
Fragments arranged by size:
2164 1729 716 246 165 17
DraIII CAC_nnn'GTG
Cuts at: 0 3238 3277 3717 4278 4404 5037
Size: 3238 39 440 561 126 633
Fragments arranged by size:
3238 633 561 440 126 39
EaeI y'GGCC_r
Cuts at: 0 441 966 1296 1721 2075 2276 2390 4303
Size: 441 525 330 425 354 201 114 1913
Cuts at: 4303 4973 5037
Size: 670 64
Fragments arranged by size:
1913 670 525 441 425 354 330 201
114 64
EagI C'GGCC_G
Cuts at: 0 4973 5037
Size: 4973 64
EarI CTCTTCn'nnn_
Cuts at: 0 1490 1507 2111 2282 2642 2729 3356 3679
Size: 1490 17 604 171 360 87 627 323
Cuts at: 3679 5037
Size: 1358
Fragments arranged by size:
1490 1358 627 604 360 323 171 87
17
EcoICRI GAG'CTC
Cuts at: 0 86 1500 3800 5037
Size: 86 1414 2300 1237
Fragments arranged by size:
2300 1414 1237 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 102 149 215 538 695 926 1089
Size: 5 97 47 66 323 157 231 163
Cuts at: 1089 1300 1796 1839 2044 2185 2443 2474 3122
Size: 211 496 43 205 141 258 31 648
Cuts at: 3122 3280 3319 3580 4867 5037
Size: 158 39 261 1287 170
Fragments arranged by size:
1287 648 496 323 261 258 231 211
205 170 163 158 157 141 97 66
47 43 39 31 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1985 5037
Size: 1985 3052
EcoO109I rG'GnC_Cy
Cuts at: 0 26 60 77 430 431 504 580 646
Size: 26 34 17 353 1 73 76 66
Cuts at: 646 663 861 1003 1306 1307 1519 1570 1776
Size: 17 198 142 303 1 212 51 206
Cuts at: 1776 1930 1990 2480 2618 2662 3015 3110 3283
Size: 154 60 490 138 44 353 95 173
Cuts at: 3283 3284 3639 4447 4448 4904 5016 5037
Size: 1 355 808 1 456 112 21
Fragments arranged by size:
808 490 456 355 353 353 303 212
206 198 173 154 142 138 112 95
76 73 66 60 51 44 34 26
21 17 17 1 1 1 1
EcoRI G'AATT_C
Cuts at: 0 2050 5037
Size: 2050 2987
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2190 3357 5037
Size: 2190 1167 1680
Fragments arranged by size:
2190 1680 1167
FspI TGC'GCA
Cuts at: 0 3376 5037
Size: 3376 1661
HaeII r_GCGC'y
Cuts at: 0 241 398 897 1256 1863 2561 4259 4815
Size: 241 157 499 359 607 698 1698 556
Cuts at: 4815 5037
Size: 222
Fragments arranged by size:
1698 698 607 556 499 359 241 222
157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 488 520 3063 3095 3565 3597 3759 3791
Size: 488 32 2543 32 470 32 162 32
Cuts at: 3791 3880 3912 5037
Size: 89 32 1125
Fragments arranged by size:
2543 1125 488 470 162 89 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 4188 5037
Size: 4188 849
HindIII A'AGCT_T
Cuts at: 0 414 1851 5037
Size: 414 1437 3186
Fragments arranged by size:
3186 1437 414
KasI G'GCGC_C
Cuts at: 0 237 1859 4811 5037
Size: 237 1622 2952 226
Fragments arranged by size:
2952 1622 237 226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1306 5037
Size: 1306 3731
MscI TGG'CCA
Cuts at: 0 968 1723 2077 4305 5037
Size: 968 755 354 2228 732
Fragments arranged by size:
2228 968 755 732 354
MslI CAynn'nnrTG
Cuts at: 0 284 365 851 2089 3628 3733 4132 4897
Size: 284 81 486 1238 1539 105 399 765
Cuts at: 4897 5037
Size: 140
Fragments arranged by size:
1539 1238 765 486 399 284 140 105
81
MspA1I CmG'CkG
Cuts at: 0 906 1351 2022 2163 2461 2548 2824 2903
Size: 906 445 671 141 298 87 276 79
Cuts at: 2903 4020 4122 4473 4926 5037
Size: 1117 102 351 453 111
Fragments arranged by size:
1117 906 671 453 445 351 298 276
141 111 102 87 79
NaeI GCC'GGC
Cuts at: 0 5000 5037
Size: 5000 37
NarI GG'CG_CC
Cuts at: 0 238 1860 4812 5037
Size: 238 1622 2952 225
Fragments arranged by size:
2952 1622 238 225
NcoI C'CATG_G
Cuts at: 0 258 1029 1293 5037
Size: 258 771 264 3744
Fragments arranged by size:
3744 771 264 258
NgoMIV G'CCGG_C
Cuts at: 0 4998 5037
Size: 4998 39
NruI TCG'CGA
Cuts at: 0 1556 5034 5037
Size: 1556 3478 3
Fragments arranged by size:
3478 1556 3
NsiI A_TGCA'T
Cuts at: 0 5 4329 5037
Size: 5 4324 708
Fragments arranged by size:
4324 708 5
NspI r_CATG'y
Cuts at: 0 3258 4077 5037
Size: 3258 819 960
Fragments arranged by size:
3258 960 819
PasI CC'CwG_GG
Cuts at: 0 724 1880 2562 2621 3226 5037
Size: 724 1156 682 59 605 1811
Fragments arranged by size:
1811 1156 724 682 605 59
PflMI CCAn_nnn'nTGG
Cuts at: 0 975 4418 5037
Size: 975 3443 619
Fragments arranged by size:
3443 975 619
PfoI T'CCnGG_A
Cuts at: 0 302 3854 4310 5037
Size: 302 3552 456 727
Fragments arranged by size:
3552 727 456 302
PmlI CAC'GTG
Cuts at: 0 4086 4539 5037
Size: 4086 453 498
Fragments arranged by size:
4086 498 453
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3789 3821 5037
Size: 3789 32 1216
Fragments arranged by size:
3789 1216 32
PpuMI rG'GwC_Cy
Cuts at: 0 504 580 1519 1776 3015 5037
Size: 504 76 939 257 1239 2022
Fragments arranged by size:
2022 1239 939 504 257 76
PspOMI G'GGCC_C
Cuts at: 0 26 430 861 1306 2479 2617 2624 2661
Size: 26 404 431 445 1173 138 7 37
Cuts at: 2661 3283 4447 4904 5037
Size: 622 1164 457 133
Fragments arranged by size:
1173 1164 622 457 445 431 404 138
133 37 26 7
PstI C_TGCA'G
Cuts at: 0 452 636 2189 3062 3599 3869 4025 4616
Size: 452 184 1553 873 537 270 156 591
Cuts at: 4616 4832 4953 5037
Size: 216 121 84
Fragments arranged by size:
1553 873 591 537 452 270 216 184
156 121 84
PvuII CAG'CTG
Cuts at: 0 2461 2903 4020 4122 4473 5037
Size: 2461 442 1117 102 351 564
Fragments arranged by size:
2461 1117 564 442 351 102
SacI G_AGCT'C
Cuts at: 0 88 1502 3802 5037
Size: 88 1414 2300 1235
Fragments arranged by size:
2300 1414 1235 88
SanDI GG'GwC_CC
Cuts at: 0 1519 5037
Size: 1519 3518
SapI GCTCTTCn'nnn_
Cuts at: 0 1490 1507 2642 3356 5037
Size: 1490 17 1135 714 1681
Fragments arranged by size:
1681 1490 1135 714 17
SbfI CC_TGCA'GG
Cuts at: 0 4832 5037
Size: 4832 205
ScaI AGT'ACT
Cuts at: 0 2437 5037
Size: 2437 2600
SexAI A'CCwGG_T
Cuts at: 0 4784 5037
Size: 4784 253
SfcI C'TryA_G
Cuts at: 0 448 632 2185 3058 3595 3865 4021 4612
Size: 448 184 1553 873 537 270 156 591
Cuts at: 4612 4828 4949 5037
Size: 216 121 88
Fragments arranged by size:
1553 873 591 537 448 270 216 184
156 121 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3118 5037
Size: 3118 1919
SfoI GGC'GCC
Cuts at: 0 239 1861 4813 5037
Size: 239 1622 2952 224
Fragments arranged by size:
2952 1622 239 224
SmaI CCC'GGG
Cuts at: 0 1965 2629 2660 3523 5037
Size: 1965 664 31 863 1514
Fragments arranged by size:
1965 1514 863 664 31
SmlI C'TyrA_G
Cuts at: 0 544 5037
Size: 544 4493
SspI AAT'ATT
Cuts at: 0 4335 5037
Size: 4335 702
StuI AGG'CCT
Cuts at: 0 3844 4826 4879 5037
Size: 3844 982 53 158
Fragments arranged by size:
3844 982 158 53
StyI C'CwwG_G
Cuts at: 0 54 258 500 1029 1293 2408 2864 3005
Size: 54 204 242 529 264 1115 456 141
Cuts at: 3005 3011 3116 3290 3437 5037
Size: 6 105 174 147 1600
Fragments arranged by size:
1600 1115 529 456 264 242 204 174
147 141 105 54 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 938 2296 2692 3144 3561 3745 4392 5037
Size: 938 1358 396 452 417 184 647 645
Fragments arranged by size:
1358 938 647 645 452 417 396 184
TatI w'GTAC_w
Cuts at: 0 2435 3604 4124 5037
Size: 2435 1169 520 913
Fragments arranged by size:
2435 1169 913 520
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 511 543 1529 1561 2211 2243 4358 4390
Size: 511 32 986 32 650 32 2115 32
Cuts at: 4390 5037
Size: 647
Fragments arranged by size:
2115 986 650 647 511 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1439 3555 3687 3690 5037
Size: 1439 2116 132 3 1347
Fragments arranged by size:
2116 1439 1347 132 3
XmaI C'CCGG_G
Cuts at: 0 1963 2627 2658 3521 5037
Size: 1963 664 31 863 1516
Fragments arranged by size:
1963 1516 863 664 31
XmnI GAAnn'nnTTC
Cuts at: 0 1333 2105 5037
Size: 1333 772 2932
Fragments arranged by size:
2932 1333 772
ZraI GAC'GTC
Cuts at: 0 3414 5037
Size: 3414 1623
Enzymes that do cut:
AatII AcuI AfeI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AseI AvaI BanI BanII BbeI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PspOMI PstI PvuII SacI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SmaI SmlI SspI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AscI
AsiSI AvrII BaeI BamHI BbsI BciVI BmtI BplI
BsaBI BsaWI BsiWI BsmBI BspEI BstBI BstEII BstZ17I
ClaI CspCI DraI DrdI EciI EcoRV FseI FspAI
HpaI KpnI MfeI MluI NdeI NheI NotI PacI
PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SgfI SgrAI SnaBI SpeI SphI SrfI
SwaI Tth111I XbaI XhoI