Restriction map for
pF1KE3717
(Linear) (Six-base) MAPSORT of: KIEE3717.seq Check: 6894 from: 1 to: 7146
KIEE3717.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AatII G_ACGT'C
Cuts at: 0 916 6718 7146
Size: 916 5802 428
Fragments arranged by size:
5802 916 428
AccI GT'mk_AC
Cuts at: 0 704 2554 3203 3624 3906 6304 6761 7146
Size: 704 1850 649 421 282 2398 457 385
Fragments arranged by size:
2398 1850 704 649 457 421 385 282
Acc65I G'GTAC_C
Cuts at: 0 1153 7146
Size: 1153 5993
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 988 1141 1370 1841 1847 1907 3212
Size: 808 180 153 229 471 6 60 1305
Cuts at: 3212 4157 4166 4331 4352 4928 5738 6458 7146
Size: 945 9 165 21 576 810 720 688
Fragments arranged by size:
1305 945 810 808 720 688 576 471
229 180 165 153 60 21 9 6
AflII C'TTAA_G
Cuts at: 0 4017 6553 7146
Size: 4017 2536 593
Fragments arranged by size:
4017 2536 593
AhdI GACnn_n'nnGTC
Cuts at: 0 6189 7146
Size: 6189 957
AleI CACnn'nnGTG
Cuts at: 0 2459 2980 4171 4450 7146
Size: 2459 521 1191 279 2696
Fragments arranged by size:
2696 2459 1191 521 279
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2402 2434 3023 3055 3661 3693 5397 5429
Size: 2402 32 589 32 606 32 1704 32
Cuts at: 5429 7146
Size: 1717
Fragments arranged by size:
2402 1717 1704 606 589 32 32 32
32
AlwNI CAG_nnn'CTG
Cuts at: 0 418 2616 3847 4069 4375 5554 5809 6267
Size: 418 2198 1231 222 306 1179 255 458
Cuts at: 6267 7146
Size: 879
Fragments arranged by size:
2198 1231 1179 879 458 418 306 255
222
ApaI G_GGCC'C
Cuts at: 0 451 6774 6835 7146
Size: 451 6323 61 311
Fragments arranged by size:
6323 451 311 61
ApaLI G'TGCA_C
Cuts at: 0 3209 7146
Size: 3209 3937
ApoI r'AATT_y
Cuts at: 0 3362 3442 7080 7146
Size: 3362 80 3638 66
Fragments arranged by size:
3638 3362 80 66
AseI AT'TA_AT
Cuts at: 0 1690 7146
Size: 1690 5456
AvaI C'yCGr_G
Cuts at: 0 2000 2655 3466 6773 7146
Size: 2000 655 811 3307 373
Fragments arranged by size:
3307 2000 811 655 373
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1906 1939 2842 2875 7146
Size: 1906 33 903 33 4271
Fragments arranged by size:
4271 1906 903 33 33
BamHI G'GATC_C
Cuts at: 0 1978 7146
Size: 1978 5168
BanI G'GyrC_C
Cuts at: 0 10 164 980 1153 5602 6321 6604 7146
Size: 10 154 816 173 4449 719 283 542
Fragments arranged by size:
4449 816 719 542 283 173 154 10
BanII G_rGCy'C
Cuts at: 0 356 451 3868 5013 6112 6774 6835 7146
Size: 356 95 3417 1145 1099 662 61 311
Fragments arranged by size:
3417 1145 1099 662 356 311 95 61
BbeI G_GCGC'C
Cuts at: 0 14 168 7146
Size: 14 154 6978
Fragments arranged by size:
6978 154 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 814 994 1980 3692 6023 6215 6375 7146
Size: 814 180 986 1712 2331 192 160 771
Fragments arranged by size:
2331 1712 986 814 771 192 180 160
BbvCI CC'TCA_GC
Cuts at: 0 2216 2795 7146
Size: 2216 579 4351
Fragments arranged by size:
4351 2216 579
BciVI GTATCCnnnnn_n'
Cuts at: 0 4352 4807 7146
Size: 4352 455 2339
Fragments arranged by size:
4352 2339 455
BclI T'GATC_A
Cuts at: 0 1647 3606 5423 7146
Size: 1647 1959 1817 1723
Fragments arranged by size:
1959 1817 1723 1647
BfrBI ATG'CAT
Cuts at: 0 1376 7146
Size: 1376 5770
BglI GCCn_nnn'nGGC
Cuts at: 0 1082 4695 5794 7146
Size: 1082 3613 1099 1352
Fragments arranged by size:
3613 1352 1099 1082
BglII A'GATC_T
Cuts at: 0 1289 7146
Size: 1289 5857
BlpI GC'TnA_GC
Cuts at: 0 4525 5012 6106 6698 7146
Size: 4525 487 1094 592 448
Fragments arranged by size:
4525 1094 592 487 448
Bme1580I G_kGCm'C
Cuts at: 0 451 3213 3939 4133 5607 5775 6326 6733
Size: 451 2762 726 194 1474 168 551 407
Cuts at: 6733 6774 6818 6835 6964 7146
Size: 41 44 17 129 182
Fragments arranged by size:
2762 1474 726 551 451 407 194 182
168 129 44 41 17
BmgBI CAC'GTC
Cuts at: 0 3934 7146
Size: 3934 3212
BmrI ACTGGGnnnn_n'
Cuts at: 0 124 487 1928 2416 3818 5203 5355 5819
Size: 124 363 1441 488 1402 1385 152 464
Cuts at: 5819 6322 7146
Size: 503 824
Fragments arranged by size:
1441 1402 1385 824 503 488 464 363
152 124
BmtI G_CTAG'C
Cuts at: 0 1829 7146
Size: 1829 5317
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 50 2345 3344 7146
Size: 50 2295 999 3802
Fragments arranged by size:
3802 2295 999 50
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 298 395 640 2277 2834 4052 4172
Size: 69 229 97 245 1637 557 1218 120
Cuts at: 4172 4397 4679 5027 5053 6552 6617 7146
Size: 225 282 348 26 1499 65 529
Fragments arranged by size:
1637 1499 1218 557 529 348 282 245
229 225 120 97 69 65 26
Bpu10I CC'TnA_GC
Cuts at: 0 2216 2795 4406 7146
Size: 2216 579 1611 2740
Fragments arranged by size:
2740 2216 1611 579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1258 2115 2154 2315 2336 3728 5498
Size: 646 612 857 39 161 21 1392 1770
Cuts at: 5498 7146
Size: 1648
Fragments arranged by size:
1770 1648 1392 857 646 612 161 39
21
BsaI GGTCTCn'nnnn_
Cuts at: 0 361 616 1094 3380 4195 4985 5029 7146
Size: 361 255 478 2286 815 790 44 2117
Fragments arranged by size:
2286 2117 815 790 478 361 255 44
BsaAI yAC'GTr
Cuts at: 0 1513 7146
Size: 1513 5633
BsaBI GATnn'nnATC
Cuts at: 0 958 1907 6245 7146
Size: 958 949 4338 901
Fragments arranged by size:
4338 958 949 901
BsaHI Gr'CG_yC
Cuts at: 0 11 165 913 6715 7146
Size: 11 154 748 5802 431
Fragments arranged by size:
5802 748 431 154 11
BsaWI w'CCGG_w
Cuts at: 0 348 5277 6079 7146
Size: 348 4929 802 1067
Fragments arranged by size:
4929 1067 802 348
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1008 1038 1442 1472 2232 2262 2474 2504
Size: 1008 30 404 30 760 30 212 30
Cuts at: 2504 3023 3053 3377 3407 3951 3981 4527 4557
Size: 519 30 324 30 544 30 546 30
Cuts at: 4557 4652 4682 5259 5289 5543 5573 7146
Size: 95 30 577 30 254 30 1573
Fragments arranged by size:
1573 1008 760 577 546 544 519 404
324 254 212 95 30 30 30 30
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 541 1201 1744 2240 2534 3484 5924
Size: 169 372 660 543 496 294 950 2440
Cuts at: 5924 6156 7146
Size: 232 990
Fragments arranged by size:
2440 990 950 660 543 496 372 294
232 169
BseYI C'CCAG_C
Cuts at: 0 308 313 401 469 500 2384 3050 3092
Size: 308 5 88 68 31 1884 666 42
Cuts at: 3092 3176 5320 5366 6345 6437 6488 7026 7146
Size: 84 2144 46 979 92 51 538 120
Fragments arranged by size:
2144 1884 979 666 538 308 120 92
88 84 68 51 46 42 31 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1268 2284 2294 3004 4619 6488 6842
Size: 257 1011 1016 10 710 1615 1869 354
Cuts at: 6842 7146
Size: 304
Fragments arranged by size:
1869 1615 1016 1011 710 354 304 257
10
BsiEI CG_ry'CG
Cuts at: 0 128 206 7146
Size: 128 78 6940
Fragments arranged by size:
6940 128 78
BsiHKAI G_wGCw'C
Cuts at: 0 230 345 2466 3213 5013 5059 6112 7146
Size: 230 115 2121 747 1800 46 1053 1034
Fragments arranged by size:
2121 1800 1053 1034 747 230 115 46
BsmI GAATG_Cn'
Cuts at: 0 3722 7146
Size: 3722 3424
Bsp1286I G_dGCh'C
Cuts at: 0 230 345 356 451 2466 3213 3868 3939
Size: 230 115 11 95 2015 747 655 71
Cuts at: 3939 4133 5013 5059 5607 5775 6112 6326 6733
Size: 194 880 46 548 168 337 214 407
Cuts at: 6733 6774 6818 6835 6964 7146
Size: 41 44 17 129 182
Fragments arranged by size:
2015 880 747 655 548 407 337 230
214 194 182 168 129 115 95 71
46 44 41 17 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1764 4263 5299 6275 7142 7146
Size: 1764 2499 1036 976 867 4
Fragments arranged by size:
2499 1764 1036 976 867 4
BsrBI CCG'CTC
Cuts at: 0 2490 7146
Size: 2490 4656
BsrDI GCAATG_nn'
Cuts at: 0 3252 4025 7146
Size: 3252 773 3121
Fragments arranged by size:
3252 3121 773
BsrFI r'CCGG_y
Cuts at: 0 60 142 537 1072 1831 7146
Size: 60 82 395 535 759 5315
Fragments arranged by size:
5315 759 535 395 82 60
BsrGI T'GTAC_A
Cuts at: 0 4836 7146
Size: 4836 2310
BstAPI GCAn_nnn'nTGC
Cuts at: 0 5602 7146
Size: 5602 1544
BstEII G'GTnAC_C
Cuts at: 0 6448 7146
Size: 6448 698
BstXI CCAn_nnnn'nTGG
Cuts at: 0 276 633 2592 6508 7146
Size: 276 357 1959 3916 638
Fragments arranged by size:
3916 1959 638 357 276
BstYI r'GATC_y
Cuts at: 0 989 1289 1978 2417 5382 7146
Size: 989 300 689 439 2965 1764
Fragments arranged by size:
2965 1764 989 689 439 300
BstZ17I GTA'TAC
Cuts at: 0 6305 7146
Size: 6305 841
Bsu36I CC'TnA_GG
Cuts at: 0 1895 7146
Size: 1895 5251
BtgI C'CryG_G
Cuts at: 0 101 122 195 323 632 731 1658 2112
Size: 101 21 73 128 309 99 927 454
Cuts at: 2112 2762 4562 5456 7146
Size: 650 1800 894 1690
Fragments arranged by size:
1800 1690 927 894 650 454 309 128
101 99 73 21
BtsI GCAGTG_nn'
Cuts at: 0 269 7146
Size: 269 6877
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2327 2362 5843 5878 7146
Size: 2327 35 3481 35 1268
Fragments arranged by size:
3481 2327 1268 35 35
DraI TTT'AAA
Cuts at: 0 795 948 7146
Size: 795 153 6198
Fragments arranged by size:
6198 795 153
EaeI y'GGCC_r
Cuts at: 0 125 203 651 7044 7146
Size: 125 78 448 6393 102
Fragments arranged by size:
6393 448 125 102 78
EagI C'GGCC_G
Cuts at: 0 125 203 7146
Size: 125 78 6943
Fragments arranged by size:
6943 125 78
EarI CTCTTCn'nnn_
Cuts at: 0 359 608 1748 2892 4133 4653 5757 7146
Size: 359 249 1140 1144 1241 520 1104 1389
Fragments arranged by size:
1389 1241 1144 1140 1104 520 359 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 229 7146
Size: 229 6917
EcoICRI GAG'CTC
Cuts at: 0 5011 6110 7146
Size: 5011 1099 1036
Fragments arranged by size:
5011 1099 1036
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 298 395 640 808 988 1141 1370
Size: 69 229 97 245 168 180 153 229
Cuts at: 1370 1841 1847 1907 2277 2834 3212 4052 4157
Size: 471 6 60 370 557 378 840 105
Cuts at: 4157 4166 4172 4331 4352 4397 4679 4928 5027
Size: 9 6 159 21 45 282 249 99
Cuts at: 5027 5053 5738 6458 6552 6617 7146
Size: 26 685 720 94 65 529
Fragments arranged by size:
840 720 685 557 529 471 378 370
282 249 245 229 229 180 168 159
153 105 99 97 94 69 65 60
45 26 21 9 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4661 5342 7146
Size: 4661 681 1804
Fragments arranged by size:
4661 1804 681
EcoO109I rG'GnC_Cy
Cuts at: 0 448 3952 4402 4759 5347 5527 6264 6831
Size: 448 3504 450 357 588 180 737 567
Cuts at: 6831 6832 7146
Size: 1 314
Fragments arranged by size:
3504 737 588 567 450 448 357 314
180 1
EcoRV GAT'ATC
Cuts at: 0 609 6198 7146
Size: 609 5589 948
Fragments arranged by size:
5589 948 609
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4520 5980 7146
Size: 4520 1460 1166
Fragments arranged by size:
4520 1460 1166
HaeII r_GCGC'y
Cuts at: 0 14 168 569 7004 7146
Size: 14 154 401 6435 142
Fragments arranged by size:
6435 401 154 142 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1281 1313 1391 1423 1669 1701 1970 2002
Size: 1281 32 78 32 246 32 269 32
Cuts at: 2002 2142 2174 2232 2264 2474 2506 3508 3540
Size: 140 32 58 32 210 32 1002 32
Cuts at: 3540 4579 4611 5626 5658 5785 5817 6175 6207
Size: 1039 32 1015 32 127 32 358 32
Cuts at: 6207 7146
Size: 939
Fragments arranged by size:
1281 1039 1015 1002 939 358 269 246
210 140 127 78 58 32 32 32
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1209 1965 7146
Size: 1209 756 5181
Fragments arranged by size:
5181 1209 756
KasI G'GCGC_C
Cuts at: 0 10 164 7146
Size: 10 154 6982
Fragments arranged by size:
6982 154 10
KpnI G_GTAC'C
Cuts at: 0 1157 7146
Size: 1157 5989
MfeI C'AATT_G
Cuts at: 0 5395 6765 7146
Size: 5395 1370 381
Fragments arranged by size:
5395 1370 381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2713 2915 5491 5808 7077 7146
Size: 2713 202 2576 317 1269 69
Fragments arranged by size:
2713 2576 1269 317 202 69
MscI TGG'CCA
Cuts at: 0 7046 7146
Size: 7046 100
MslI CAynn'nnrTG
Cuts at: 0 2459 2590 2980 3218 4171 4450 6020 6880
Size: 2459 131 390 238 953 279 1570 860
Cuts at: 6880 7146
Size: 266
Fragments arranged by size:
2459 1570 953 860 390 279 266 238
131
MspA1I CmG'CkG
Cuts at: 0 124 325 418 554 1713 2362 2599 2647
Size: 124 201 93 136 1159 649 237 48
Cuts at: 2647 2715 4417 4512 5331 6105 6349 6540 7146
Size: 68 1702 95 819 774 244 191 606
Fragments arranged by size:
1702 1159 819 774 649 606 244 237
201 191 136 124 95 93 68 48
NaeI GCC'GGC
Cuts at: 0 62 539 7146
Size: 62 477 6607
Fragments arranged by size:
6607 477 62
NarI GG'CG_CC
Cuts at: 0 11 165 7146
Size: 11 154 6981
Fragments arranged by size:
6981 154 11
NcoI C'CATG_G
Cuts at: 0 1658 2112 2762 5456 7146
Size: 1658 454 650 2694 1690
Fragments arranged by size:
2694 1690 1658 650 454
NdeI CA'TA_TG
Cuts at: 0 2028 7146
Size: 2028 5118
NgoMIV G'CCGG_C
Cuts at: 0 60 537 7146
Size: 60 477 6609
Fragments arranged by size:
6609 477 60
NheI G'CTAG_C
Cuts at: 0 1825 7146
Size: 1825 5321
NotI GC'GGCC_GC
Cuts at: 0 125 203 7146
Size: 125 78 6943
Fragments arranged by size:
6943 125 78
NsiI A_TGCA'T
Cuts at: 0 1378 7146
Size: 1378 5768
NspI r_CATG'y
Cuts at: 0 1236 5020 7146
Size: 1236 3784 2126
Fragments arranged by size:
3784 2126 1236
PacI TTA_AT'TAA
Cuts at: 0 1694 7146
Size: 1694 5452
PasI CC'CwG_GG
Cuts at: 0 427 2071 7146
Size: 427 1644 5075
Fragments arranged by size:
5075 1644 427
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 7059 7146
Size: 180 6879 87
Fragments arranged by size:
6879 180 87
PfoI T'CCnGG_A
Cuts at: 0 4656 4938 5343 7146
Size: 4656 282 405 1803
Fragments arranged by size:
4656 1803 405 282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3023 3055 5481 5513 7146
Size: 3023 32 2426 32 1633
Fragments arranged by size:
3023 2426 1633 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 3952 4402 4759 5347 6264 7146
Size: 3952 450 357 588 917 882
Fragments arranged by size:
3952 917 882 588 450 357
PshAI GACnn'nnGTC
Cuts at: 0 1106 1970 7146
Size: 1106 864 5176
Fragments arranged by size:
5176 1106 864
PspOMI G'GGCC_C
Cuts at: 0 447 6770 6831 7146
Size: 447 6323 61 315
Fragments arranged by size:
6323 447 315 61
PstI C_TGCA'G
Cuts at: 0 758 2702 3144 4260 4380 6272 6921 7146
Size: 758 1944 442 1116 120 1892 649 225
Fragments arranged by size:
1944 1892 1116 758 649 442 225 120
PvuII CAG'CTG
Cuts at: 0 2362 2599 2647 2715 4417 4512 5331 6105
Size: 2362 237 48 68 1702 95 819 774
Cuts at: 6105 6349 6540 7146
Size: 244 191 606
Fragments arranged by size:
2362 1702 819 774 606 244 237 191
95 68 48
SacI G_AGCT'C
Cuts at: 0 5013 6112 7146
Size: 5013 1099 1034
Fragments arranged by size:
5013 1099 1034
SacII CC_GC'GG
Cuts at: 0 125 326 7146
Size: 125 201 6820
Fragments arranged by size:
6820 201 125
SapI GCTCTTCn'nnn_
Cuts at: 0 608 7146
Size: 608 6538
ScaI AGT'ACT
Cuts at: 0 2951 3114 4223 4389 4536 5124 7146
Size: 2951 163 1109 166 147 588 2022
Fragments arranged by size:
2951 2022 1109 588 166 163 147
SfcI C'TryA_G
Cuts at: 0 754 1918 2057 2698 2918 3140 3200 3854
Size: 754 1164 139 641 220 222 60 654
Cuts at: 3854 4256 4376 4418 5849 6268 6917 7146
Size: 402 120 42 1431 419 649 229
Fragments arranged by size:
1431 1164 754 654 649 641 419 402
229 222 220 139 120 60 42
SfoI GGC'GCC
Cuts at: 0 12 166 7146
Size: 12 154 6980
Fragments arranged by size:
6980 154 12
SgrAI Cr'CCGG_yG
Cuts at: 0 537 7146
Size: 537 6609
SmaI CCC'GGG
Cuts at: 0 2657 7146
Size: 2657 4489
SmlI C'TyrA_G
Cuts at: 0 661 1237 2000 2130 2169 2330 2351 3743
Size: 661 576 763 130 39 161 21 1392
Cuts at: 3743 4017 5513 6553 7146
Size: 274 1496 1040 593
Fragments arranged by size:
1496 1392 1040 763 661 593 576 274
161 130 39 21
SnaBI TAC'GTA
Cuts at: 0 1513 7146
Size: 1513 5633
SpeI A'CTAG_T
Cuts at: 0 5043 5542 7146
Size: 5043 499 1604
Fragments arranged by size:
5043 1604 499
SphI G_CATG'C
Cuts at: 0 1236 5020 7146
Size: 1236 3784 2126
Fragments arranged by size:
3784 2126 1236
SspI AAT'ATT
Cuts at: 0 837 2877 7146
Size: 837 2040 4269
Fragments arranged by size:
4269 2040 837
StuI AGG'CCT
Cuts at: 0 3916 7146
Size: 3916 3230
StyI C'CwwG_G
Cuts at: 0 569 626 1658 2112 2403 2762 2781 4805
Size: 569 57 1032 454 291 359 19 2024
Cuts at: 4805 5456 5708 5822 6419 7146
Size: 651 252 114 597 727
Fragments arranged by size:
2024 1032 727 651 597 569 454 359
291 252 114 57 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 1096 2747 3010 3404 6886 7146
Size: 166 930 1651 263 394 3482 260
Fragments arranged by size:
3482 1651 930 394 263 260 166
TatI w'GTAC_w
Cuts at: 0 158 2949 3112 4221 4330 4387 4534 4606
Size: 158 2791 163 1109 109 57 147 72
Cuts at: 4606 4836 5122 7146
Size: 230 286 2024
Fragments arranged by size:
2791 2024 1109 286 230 163 158 147
109 72 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3951 3983 4280 4312 4652 4684 5057 5089
Size: 3951 32 297 32 340 32 373 32
Cuts at: 5089 7146
Size: 2057
Fragments arranged by size:
3951 2057 373 340 297 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 4962 5376 7146
Size: 4962 414 1770
Fragments arranged by size:
4962 1770 414
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4170 7146
Size: 4170 2976
XhoI C'TCGA_G
Cuts at: 0 2000 7146
Size: 2000 5146
XmaI C'CCGG_G
Cuts at: 0 2655 7146
Size: 2655 4491
XmnI GAAnn'nnTTC
Cuts at: 0 6414 7146
Size: 6414 732
ZraI GAC'GTC
Cuts at: 0 914 6716 7146
Size: 914 5802 430
Fragments arranged by size:
5802 914 430
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AhdI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I HincII KasI KpnI MfeI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PacI PasI PflMI PfoI
PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII
SapI ScaI SfcI SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflIII AgeI AlfI AscI AsiSI
AvrII BcgI BsiWI BsmBI BspEI BspHI BssHII BssSI
BstBI BtgZI ClaI DraIII DrdI EcoRI FseI FspI
FspAI HindIII HpaI MluI NruI PciI PmeI PmlI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI
SexAI SfiI SgfI SrfI SwaI Tth111I