Restriction map for
pF1KE3911
(Linear) (Six-base) MAPSORT of: KIEE3911.seq Check: 5830 from: 1 to: 2130
KIEE3911.orf
With 182 enzymes: SgfI *
November 15, 2013 14:41 ..
AccI GT'mk_AC
Cuts at: 0 1085 2130
Size: 1085 1045
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 1300 1492 2130
Size: 38 1262 192 638
Fragments arranged by size:
1262 638 192 38
AflIII A'CryG_T
Cuts at: 0 1040 1685 2130
Size: 1040 645 445
Fragments arranged by size:
1040 645 445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 608 802 1222 2130
Size: 608 194 420 908
Fragments arranged by size:
908 608 420 194
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 817 849 2130
Size: 817 32 1281
Fragments arranged by size:
1281 817 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1677 2130
Size: 1677 453
ApaLI G'TGCA_C
Cuts at: 0 1311 1858 2130
Size: 1311 547 272
Fragments arranged by size:
1311 547 272
ApoI r'AATT_y
Cuts at: 0 183 1547 2130
Size: 183 1364 583
Fragments arranged by size:
1364 583 183
AvaI C'yCGr_G
Cuts at: 0 399 418 766 2130
Size: 399 19 348 1364
Fragments arranged by size:
1364 399 348 19
BanI G'GyrC_C
Cuts at: 0 1440 2130
Size: 1440 690
BanII G_rGCy'C
Cuts at: 0 302 372 530 1187 1904 2130
Size: 302 70 158 657 717 226
Fragments arranged by size:
717 657 302 226 158 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 611 706 2130
Size: 611 95 1424
Fragments arranged by size:
1424 611 95
BbvCI CC'TCA_GC
Cuts at: 0 2078 2130
Size: 2078 52
BclI T'GATC_A
Cuts at: 0 54 1400 2130
Size: 54 1346 730
Fragments arranged by size:
1346 730 54
BglII A'GATC_T
Cuts at: 0 713 2009 2130
Size: 713 1296 121
Fragments arranged by size:
1296 713 121
BlpI GC'TnA_GC
Cuts at: 0 1874 2130
Size: 1874 256
Bme1580I G_kGCm'C
Cuts at: 0 1315 1445 1862 2130
Size: 1315 130 417 268
Fragments arranged by size:
1315 417 268 130
BmgBI CAC'GTC
Cuts at: 0 969 1041 2130
Size: 969 72 1089
Fragments arranged by size:
1089 969 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 408 719 2130
Size: 408 311 1411
Fragments arranged by size:
1411 408 311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 522 1018 1024 1126 1678 1726 1918
Size: 342 180 496 6 102 552 48 192
Cuts at: 1918 2130
Size: 212
Fragments arranged by size:
552 496 342 212 192 180 102 48
6
Bpu10I CC'TnA_GC
Cuts at: 0 2078 2130
Size: 2078 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 723 811 1240 2026 2130
Size: 110 613 88 429 786 104
Fragments arranged by size:
786 613 429 110 104 88
BsaAI yAC'GTr
Cuts at: 0 1869 2130
Size: 1869 261
BsaHI Gr'CG_yC
Cuts at: 0 856 1370 1747 2130
Size: 856 514 377 383
Fragments arranged by size:
856 514 383 377
BsaWI w'CCGG_w
Cuts at: 0 773 1010 2027 2130
Size: 773 237 1017 103
Fragments arranged by size:
1017 773 237 103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 817 847 2130
Size: 817 30 1283
Fragments arranged by size:
1283 817 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 436 661 676 679 682 685 688
Size: 130 306 225 15 3 3 3 3
Cuts at: 688 823 1033 1206 1666 2020 2130
Size: 135 210 173 460 354 110
Fragments arranged by size:
460 354 306 225 210 173 135 130
110 15 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1990 2109 2130
Size: 1990 119 21
Fragments arranged by size:
1990 119 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1218 1948 2130
Size: 910 308 730 182
Fragments arranged by size:
910 730 308 182
BsiHKAI G_wGCw'C
Cuts at: 0 1010 1187 1315 1862 1904 2130
Size: 1010 177 128 547 42 226
Fragments arranged by size:
1010 547 226 177 128 42
BsmI GAATG_Cn'
Cuts at: 0 1406 2130
Size: 1406 724
Bsp1286I G_dGCh'C
Cuts at: 0 302 372 530 1010 1187 1315 1445 1862
Size: 302 70 158 480 177 128 130 417
Cuts at: 1862 1904 2130
Size: 42 226
Fragments arranged by size:
480 417 302 226 177 158 130 128
70 42
BspEI T'CCGG_A
Cuts at: 0 773 2130
Size: 773 1357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 887 1580 2130
Size: 887 693 550
Fragments arranged by size:
887 693 550
BsrBI CCG'CTC
Cuts at: 0 909 987 1122 1305 2130
Size: 909 78 135 183 825
Fragments arranged by size:
909 825 183 135 78
BsrDI GCAATG_nn'
Cuts at: 0 159 356 2130
Size: 159 197 1774
Fragments arranged by size:
1774 197 159
BsrFI r'CCGG_y
Cuts at: 0 429 678 1586 2130
Size: 429 249 908 544
Fragments arranged by size:
908 544 429 249
BssSI C'ACGA_G
Cuts at: 0 883 2130
Size: 883 1247
BstXI CCAn_nnnn'nTGG
Cuts at: 0 261 963 2130
Size: 261 702 1167
Fragments arranged by size:
1167 702 261
BstYI r'GATC_y
Cuts at: 0 713 759 770 794 920 1946 2009 2024
Size: 713 46 11 24 126 1026 63 15
Cuts at: 2024 2130
Size: 106
Fragments arranged by size:
1026 713 126 106 63 46 24 15
11
Bsu36I CC'TnA_GG
Cuts at: 0 806 1522 2130
Size: 806 716 608
Fragments arranged by size:
806 716 608
BtgI C'CryG_G
Cuts at: 0 1630 2130
Size: 1630 500
BtsI GCAGTG_nn'
Cuts at: 0 1313 2130
Size: 1313 817
EaeI y'GGCC_r
Cuts at: 0 427 437 626 680 1679 2130
Size: 427 10 189 54 999 451
Fragments arranged by size:
999 451 427 189 54 10
EarI CTCTTCn'nnn_
Cuts at: 0 653 955 1276 1286 1543 1561 2130
Size: 653 302 321 10 257 18 569
Fragments arranged by size:
653 569 321 302 257 18 10
EcoICRI GAG'CTC
Cuts at: 0 1185 1902 2130
Size: 1185 717 228
Fragments arranged by size:
1185 717 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 342 522 1018 1024 1126 1300 1492
Size: 38 304 180 496 6 102 174 192
Cuts at: 1492 1678 1726 1918 2130
Size: 186 48 192 212
Fragments arranged by size:
496 304 212 192 192 186 180 174
102 48 38 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 535 754 2130
Size: 535 219 1376
Fragments arranged by size:
1376 535 219
EcoO109I rG'GnC_Cy
Cuts at: 0 465 620 1501 2130
Size: 465 155 881 629
Fragments arranged by size:
881 629 465 155
EcoRV GAT'ATC
Cuts at: 0 141 2130
Size: 141 1989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1554 2130
Size: 1554 576
FseI GG_CCGG'CC
Cuts at: 0 433 2130
Size: 433 1697
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1063 1095 2001 2033 2130
Size: 1063 32 906 32 97
Fragments arranged by size:
1063 906 97 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 1753 2130
Size: 980 773 377
Fragments arranged by size:
980 773 377
MscI TGG'CCA
Cuts at: 0 1681 2130
Size: 1681 449
MslI CAynn'nnrTG
Cuts at: 0 1735 2130
Size: 1735 395
MspA1I CmG'CkG
Cuts at: 0 257 782 1137 1463 1632 2130
Size: 257 525 355 326 169 498
Fragments arranged by size:
525 498 355 326 257 169
NaeI GCC'GGC
Cuts at: 0 431 680 1588 2130
Size: 431 249 908 542
Fragments arranged by size:
908 542 431 249
NgoMIV G'CCGG_C
Cuts at: 0 429 678 1586 2130
Size: 429 249 908 544
Fragments arranged by size:
908 544 429 249
NspI r_CATG'y
Cuts at: 0 1361 1740 2130
Size: 1361 379 390
Fragments arranged by size:
1361 390 379
PflMI CCAn_nnn'nTGG
Cuts at: 0 897 1677 2067 2130
Size: 897 780 390 63
Fragments arranged by size:
897 780 390 63
PfoI T'CCnGG_A
Cuts at: 0 499 962 995 1142 1160 1513 1596 1922
Size: 499 463 33 147 18 353 83 326
Cuts at: 1922 2130
Size: 208
Fragments arranged by size:
499 463 353 326 208 147 83 33
18
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 817 849 2130
Size: 817 32 1281
Fragments arranged by size:
1281 817 32
PpuMI rG'GwC_Cy
Cuts at: 0 620 2130
Size: 620 1510
PstI C_TGCA'G
Cuts at: 0 824 1490 2130
Size: 824 666 640
Fragments arranged by size:
824 666 640
PvuII CAG'CTG
Cuts at: 0 257 1137 2130
Size: 257 880 993
Fragments arranged by size:
993 880 257
SacI G_AGCT'C
Cuts at: 0 1187 1904 2130
Size: 1187 717 226
Fragments arranged by size:
1187 717 226
SacII CC_GC'GG
Cuts at: 0 1633 2130
Size: 1633 497
SanDI GG'GwC_CC
Cuts at: 0 620 2130
Size: 620 1510
SapI GCTCTTCn'nnn_
Cuts at: 0 1543 2130
Size: 1543 587
SexAI A'CCwGG_T
Cuts at: 0 1610 2130
Size: 1610 520
SfcI C'TryA_G
Cuts at: 0 799 820 1486 2130
Size: 799 21 666 644
Fragments arranged by size:
799 666 644 21
SmaI CCC'GGG
Cuts at: 0 401 2130
Size: 401 1729
SmlI C'TyrA_G
Cuts at: 0 125 738 790 1255 2041 2130
Size: 125 613 52 465 786 89
Fragments arranged by size:
786 613 465 125 89 52
SphI G_CATG'C
Cuts at: 0 1361 2130
Size: 1361 769
StyI C'CwwG_G
Cuts at: 0 83 224 2130
Size: 83 141 1906
Fragments arranged by size:
1906 141 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1877 2130
Size: 1877 253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 837 2130
Size: 837 1293
XmaI C'CCGG_G
Cuts at: 0 399 2130
Size: 399 1731
XmnI GAAnn'nnTTC
Cuts at: 0 1537 2130
Size: 1537 593
Enzymes that do cut:
AccI AcuI AflIII AlfI AloI AlwNI ApaLI ApoI
AvaI BanI BanII BbsI BbvCI BclI BglII BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI FseI Hin4I MmeI MscI MslI
MspA1I NaeI NgoMIV NspI PflMI PfoI PpiI PpuMI
PstI PvuII SacI SacII SanDI SapI SexAI SfcI
SmaI SmlI SphI StyI TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BfrBI BglI BmrI BmtI BsaI
BsaBI BsiEI BsiWI BsmBI BspHI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoRI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XhoI ZraI