Restriction map for
pF1KE3971
(Linear) (Six-base) MAPSORT of: KIEE3971.seq Check: 1370 from: 1 to: 1362
KIEE3971.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 0 546 1362
Size: 546 816
Acc65I G'GTAC_C
Cuts at: 0 430 1362
Size: 430 932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 590 1112 1362
Size: 527 63 522 250
Fragments arranged by size:
527 522 250 63
AflII C'TTAA_G
Cuts at: 0 850 1362
Size: 850 512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 255 416 1362
Size: 255 161 946
Fragments arranged by size:
946 255 161
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 103 135 1362
Size: 103 32 1227
Fragments arranged by size:
1227 103 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1255 1362
Size: 1255 107
ApaI G_GGCC'C
Cuts at: 0 43 72 1362
Size: 43 29 1290
Fragments arranged by size:
1290 43 29
ApoI r'AATT_y
Cuts at: 0 1066 1362
Size: 1066 296
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 414 447 1362
Size: 414 33 915
Fragments arranged by size:
915 414 33
BanI G'GyrC_C
Cuts at: 0 49 430 1362
Size: 49 381 932
Fragments arranged by size:
932 381 49
BanII G_rGCy'C
Cuts at: 0 43 72 223 1362
Size: 43 29 151 1139
Fragments arranged by size:
1139 151 43 29
BbeI G_GCGC'C
Cuts at: 0 53 1362
Size: 53 1309
BciVI GTATCCnnnnn_n'
Cuts at: 0 93 1362
Size: 93 1269
BclI T'GATC_A
Cuts at: 0 360 792 1362
Size: 360 432 570
Fragments arranged by size:
570 432 360
BglI GCCn_nnn'nGGC
Cuts at: 0 47 1362
Size: 47 1315
Bme1580I G_kGCm'C
Cuts at: 0 43 72 1285 1362
Size: 43 29 1213 77
Fragments arranged by size:
1213 77 43 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 246 1362
Size: 246 1116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 130 301 547 1362
Size: 121 9 171 246 815
Fragments arranged by size:
815 246 171 121 9
Bpu10I CC'TnA_GC
Cuts at: 0 217 1362
Size: 217 1145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1362
Size: 481 881
BsaI GGTCTCn'nnnn_
Cuts at: 0 1268 1362
Size: 1268 94
BsaBI GATnn'nnATC
Cuts at: 0 143 1362
Size: 143 1219
BsaHI Gr'CG_yC
Cuts at: 0 50 1362
Size: 50 1312
BsaWI w'CCGG_w
Cuts at: 0 92 1362
Size: 92 1270
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 274 304 1362
Size: 103 30 141 30 1058
Fragments arranged by size:
1058 141 103 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 835 1070 1362
Size: 835 235 292
Fragments arranged by size:
835 292 235
BseYI C'CCAG_C
Cuts at: 0 21 1362
Size: 21 1341
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 949 1318 1362
Size: 361 588 369 44
Fragments arranged by size:
588 369 361 44
BsiHKAI G_wGCw'C
Cuts at: 0 223 1362
Size: 223 1139
Bsp1286I G_dGCh'C
Cuts at: 0 43 72 223 1285 1362
Size: 43 29 151 1062 77
Fragments arranged by size:
1062 151 77 43 29
BspHI T'CATG_A
Cuts at: 0 672 861 1362
Size: 672 189 501
Fragments arranged by size:
672 501 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 675 951 1362
Size: 675 276 411
Fragments arranged by size:
675 411 276
BstEII G'GTnAC_C
Cuts at: 0 1180 1362
Size: 1180 182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 603 823 1362
Size: 603 220 539
Fragments arranged by size:
603 539 220
BstYI r'GATC_y
Cuts at: 0 144 297 926 1362
Size: 144 153 629 436
Fragments arranged by size:
629 436 153 144
Bsu36I CC'TnA_GG
Cuts at: 0 1006 1362
Size: 1006 356
BtgI C'CryG_G
Cuts at: 0 62 1362
Size: 62 1300
BtsI GCAGTG_nn'
Cuts at: 0 1283 1362
Size: 1283 79
ClaI AT'CG_AT
Cuts at: 0 164 1362
Size: 164 1198
EcoICRI GAG'CTC
Cuts at: 0 221 1362
Size: 221 1141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 130 301 527 547 590 1112 1362
Size: 121 9 171 226 20 43 522 250
Fragments arranged by size:
522 250 226 171 121 43 20 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 850 1362
Size: 850 512
EcoO109I rG'GnC_Cy
Cuts at: 0 39 68 69 664 1362
Size: 39 29 1 595 698
Fragments arranged by size:
698 595 39 29 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 514 1362
Size: 514 848
HaeII r_GCGC'y
Cuts at: 0 53 1362
Size: 53 1309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 1125 1157 1362
Size: 289 32 804 32 205
Fragments arranged by size:
804 289 205 32 32
HincII GTy'rAC
Cuts at: 0 993 1002 1362
Size: 993 9 360
Fragments arranged by size:
993 360 9
HindIII A'AGCT_T
Cuts at: 0 128 679 1362
Size: 128 551 683
Fragments arranged by size:
683 551 128
HpaI GTT'AAC
Cuts at: 0 1002 1362
Size: 1002 360
KasI G'GCGC_C
Cuts at: 0 49 1362
Size: 49 1313
KpnI G_GTAC'C
Cuts at: 0 434 1362
Size: 434 928
MfeI C'AATT_G
Cuts at: 0 640 1362
Size: 640 722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1362
Size: 654 708
MslI CAynn'nnrTG
Cuts at: 0 1268 1362
Size: 1268 94
MspA1I CmG'CkG
Cuts at: 0 375 390 1362
Size: 375 15 972
Fragments arranged by size:
972 375 15
NarI GG'CG_CC
Cuts at: 0 50 1362
Size: 50 1312
PasI CC'CwG_GG
Cuts at: 0 43 1362
Size: 43 1319
PflMI CCAn_nnn'nTGG
Cuts at: 0 279 1362
Size: 279 1083
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 1362
Size: 103 32 1227
Fragments arranged by size:
1227 103 32
PpuMI rG'GwC_Cy
Cuts at: 0 664 1362
Size: 664 698
PspOMI G'GGCC_C
Cuts at: 0 39 68 1362
Size: 39 29 1294
Fragments arranged by size:
1294 39 29
PstI C_TGCA'G
Cuts at: 0 395 745 1362
Size: 395 350 617
Fragments arranged by size:
617 395 350
PvuII CAG'CTG
Cuts at: 0 375 390 1362
Size: 375 15 972
Fragments arranged by size:
972 375 15
SacI G_AGCT'C
Cuts at: 0 223 1362
Size: 223 1139
ScaI AGT'ACT
Cuts at: 0 601 1362
Size: 601 761
SfcI C'TryA_G
Cuts at: 0 391 741 1362
Size: 391 350 621
Fragments arranged by size:
621 391 350
SfoI GGC'GCC
Cuts at: 0 51 1362
Size: 51 1311
SmlI C'TyrA_G
Cuts at: 0 460 850 1362
Size: 460 390 512
Fragments arranged by size:
512 460 390
StuI AGG'CCT
Cuts at: 0 869 1362
Size: 869 493
StyI C'CwwG_G
Cuts at: 0 816 870 1362
Size: 816 54 492
Fragments arranged by size:
816 492 54
TatI w'GTAC_w
Cuts at: 0 354 599 1362
Size: 354 245 763
Fragments arranged by size:
763 354 245
XmnI GAAnn'nnTTC
Cuts at: 0 118 539 1362
Size: 118 421 823
Fragments arranged by size:
823 421 118
Enzymes that do cut:
AccI Acc65I AcuI AflII AlfI AloI AlwNI ApaI
ApoI BaeI BanI BanII BbeI BciVI BclI BglI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MmeI MslI MspA1I
NarI PasI PflMI PpiI PpuMI PspOMI PstI PvuII
SacI ScaI SfcI SfoI SmlI StuI StyI TatI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AleI
ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbsI
BbvCI BcgI BfrBI BglII BlpI BmgBI BmtI BplI
BsaAI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoRI EcoRV FseI FspI FspAI MluI MscI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI ZraI