(Linear) (Six-base) MAPSORT of: KIEE4045.seq  Check: 8585  from: 1  to: 801



KIEE4045.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595     801 
   Size:        595     206
 
AflII C'TTAA_G

Cuts at:      0     361     801 
   Size:        361     440
 
AlwNI CAG_nnn'CTG

Cuts at:      0      15      42     271     801 
   Size:         15      27     229     530

  Fragments arranged by size: 

                 530     229      27      15
 
BanII G_rGCy'C

Cuts at:      0     392     801 
   Size:        392     409
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     475     632     797     801 
   Size:        475     157     165       4

  Fragments arranged by size: 

                 475     165     157       4
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      73     107     801 
   Size:         73      34     694

  Fragments arranged by size: 

                 694      73      34
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     720     801 
   Size:        720      81
 
BseYI C'CCAG_C

Cuts at:      0     399     801 
   Size:        399     402
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     801 
   Size:        317     484
 
BsiEI CG_ry'CG

Cuts at:      0      93     801 
   Size:         93     708
 
Bsp1286I G_dGCh'C

Cuts at:      0     392     801 
   Size:        392     409
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      42     801 
   Size:         42     759
 
BstYI r'GATC_y

Cuts at:      0     268     550     801 
   Size:        268     282     251

  Fragments arranged by size: 

                 282     268     251
 
BtgI C'CryG_G

Cuts at:      0      78     116     801 
   Size:         78      38     685

  Fragments arranged by size: 

                 685      78      38
 
BtsI GCAGTG_nn'

Cuts at:      0      91     157     801 
   Size:         91      66     644

  Fragments arranged by size: 

                 644      91      66
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595     801 
   Size:        595     206
 
HindIII A'AGCT_T

Cuts at:      0     198     801 
   Size:        198     603
 
MspA1I CmG'CkG

Cuts at:      0     403     801 
   Size:        403     398
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     184     801 
   Size:        184     617
 
PfoI T'CCnGG_A

Cuts at:      0     679     801 
   Size:        679     122
 
PvuI CG_AT'CG

Cuts at:      0      93     801 
   Size:         93     708
 
PvuII CAG'CTG

Cuts at:      0     403     801 
   Size:        403     398
 
SmlI C'TyrA_G

Cuts at:      0     361     801 
   Size:        361     440
 
SspI AAT'ATT

Cuts at:      0     137     593     801 
   Size:        137     456     208

  Fragments arranged by size: 

                 456     208     137
 
XmnI GAAnn'nnTTC

Cuts at:      0     236     801 
   Size:        236     565
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI    BanII     BbsI     BcgI     BsaI    BseYI
     BsgI    BsiEI Bsp1286I   BstAPI    BstYI     BtgI     BtsI  Eco57MI
  HindIII   MspA1I    PflMI     PfoI     PvuI    PvuII     SmlI     SspI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI