(Linear) (Six-base) MAPSORT of: KIEE4105.seq  Check: 1620  from: 1  to: 1425



KIEE4105.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

AccI GT'mk_AC

Cuts at:      0     307    1358    1425 
   Size:        307    1051      67

  Fragments arranged by size: 

                1051     307      67
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1084    1425 
   Size:       1084     341
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     658     737    1425 
   Size:        658      79     688

  Fragments arranged by size: 

                 688     658      79
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1258    1290    1425 
   Size:       1258      32     135

  Fragments arranged by size: 

                1258     135      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      67     372    1425 
   Size:         67     305    1053

  Fragments arranged by size: 

                1053     305      67
 
ApoI r'AATT_y

Cuts at:      0      15      42     343     830     870    1014    1425 
   Size:         15      27     301     487      40     144     411

  Fragments arranged by size: 

                 487     411     301     144      40      27      15
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      14      47     467     500    1425 
   Size:         14      33     420      33     925

  Fragments arranged by size: 

                 925     420      33      33      14
 
BanII G_rGCy'C

Cuts at:      0    1382    1425 
   Size:       1382      43
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     789    1425 
   Size:        789     636
 
BfrBI ATG'CAT

Cuts at:      0     902    1425 
   Size:        902     523
 
BglII A'GATC_T

Cuts at:      0    1403    1425 
   Size:       1403      22
 
BlpI GC'TnA_GC

Cuts at:      0     112    1376    1425 
   Size:        112    1264      49

  Fragments arranged by size: 

                1264     112      49
 
Bme1580I G_kGCm'C

Cuts at:      0     395    1425 
   Size:        395    1030
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     398    1425 
   Size:        398    1027
 
Bpu10I CC'TnA_GC

Cuts at:      0    1088    1425 
   Size:       1088     337
 
BsaWI w'CCGG_w

Cuts at:      0    1054    1425 
   Size:       1054     371
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1175    1205    1425 
   Size:       1175      30     220

  Fragments arranged by size: 

                1175     220      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     832    1425 
   Size:        832     593
 
BsiHKAI G_wGCw'C

Cuts at:      0     426     689    1062    1382    1425 
   Size:        426     263     373     320      43

  Fragments arranged by size: 

                 426     373     320     263      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     395     426     689    1062    1382    1425 
   Size:        395      31     263     373     320      43

  Fragments arranged by size: 

                 395     373     320     263      43      31
 
BsrBI CCG'CTC

Cuts at:      0    1095    1425 
   Size:       1095     330
 
BsrDI GCAATG_nn'

Cuts at:      0     904    1425 
   Size:        904     521
 
BsrGI T'GTAC_A

Cuts at:      0    1182    1425 
   Size:       1182     243
 
BstYI r'GATC_y

Cuts at:      0     625    1403    1425 
   Size:        625     778      22

  Fragments arranged by size: 

                 778     625      22
 
BtgI C'CryG_G

Cuts at:      0      29     883    1425 
   Size:         29     854     542

  Fragments arranged by size: 

                 854     542      29
 
DraIII CAC_nnn'GTG

Cuts at:      0     855    1425 
   Size:        855     570
 
EaeI y'GGCC_r

Cuts at:      0    1250    1425 
   Size:       1250     175
 
EarI CTCTTCn'nnn_

Cuts at:      0     998    1102    1425 
   Size:        998     104     323

  Fragments arranged by size: 

                 998     323     104
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1187    1425 
   Size:       1187     238
 
EcoICRI GAG'CTC

Cuts at:      0    1380    1425 
   Size:       1380      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1084    1425 
   Size:       1084     341
 
EcoRI G'AATT_C

Cuts at:      0      42    1425 
   Size:         42    1383
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     483    1425 
   Size:        483     942
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     977    1009    1425 
   Size:        977      32     416

  Fragments arranged by size: 

                 977     416      32
 
HincII GTy'rAC

Cuts at:      0     996    1425 
   Size:        996     429
 
HindIII A'AGCT_T

Cuts at:      0    1284    1425 
   Size:       1284     141
 
MfeI C'AATT_G

Cuts at:      0     560    1425 
   Size:        560     865
 
MscI TGG'CCA

Cuts at:      0    1252    1425 
   Size:       1252     173
 
MslI CAynn'nnrTG

Cuts at:      0     719    1425 
   Size:        719     706
 
NsiI A_TGCA'T

Cuts at:      0     904    1425 
   Size:        904     521
 
PfoI T'CCnGG_A

Cuts at:      0     313    1076    1425 
   Size:        313     763     349

  Fragments arranged by size: 

                 763     349     313
 
SacI G_AGCT'C

Cuts at:      0    1382    1425 
   Size:       1382      43
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1102    1425 
   Size:       1102     323
 
SfcI C'TryA_G

Cuts at:      0     505    1425 
   Size:        505     920
 
StyI C'CwwG_G

Cuts at:      0      54     440    1425 
   Size:         54     386     985

  Fragments arranged by size: 

                 985     386      54
 
TatI w'GTAC_w

Cuts at:      0    1182    1394    1425 
   Size:       1182     212      31

  Fragments arranged by size: 

                1182     212      31
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     709     741    1425 
   Size:        709      32     684

  Fragments arranged by size: 

                 709     684      32
 
XbaI T'CTAG_A

Cuts at:      0     927    1425 
   Size:        927     498
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1284    1425 
   Size:       1284     141
 
XmnI GAAnn'nnTTC

Cuts at:      0     786    1425 
   Size:        786     639
 

 Enzymes that do cut:

     AccI     AcuI     AlfI     AloI    AlwNI     ApoI     BaeI    BanII
     BbsI    BfrBI    BglII     BlpI Bme1580I     BmrI   Bpu10I    BsaWI
    BsaXI     BsgI  BsiHKAI Bsp1286I    BsrBI    BsrDI    BsrGI    BstYI
     BtgI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoRI
     FalI    Hin4I   HincII  HindIII     MfeI     MscI     MslI     NsiI
     PfoI     SacI     SapI     SfcI     StyI     TatI     TstI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
     BglI    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EagI    EcoNI EcoO109I    EcoRV     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MluI     MmeI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XhoI
     XmaI     ZraI