Restriction map for
pF1KE4189
(Linear) (Six-base) MAPSORT of: KIEE4189.seq Check: 4356 from: 1 to: 2025
KIEE4189.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 0 359 2025
Size: 359 1666
Acc65I G'GTAC_C
Cuts at: 0 521 2025
Size: 521 1504
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 571 781 877 891 1350 2025
Size: 458 113 210 96 14 459 675
Fragments arranged by size:
675 459 458 210 113 96 14
AflIII A'CryG_T
Cuts at: 0 398 2025
Size: 398 1627
AleI CACnn'nnGTG
Cuts at: 0 1270 2025
Size: 1270 755
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 196 228 2025
Size: 196 32 1797
Fragments arranged by size:
1797 196 32
AlwNI CAG_nnn'CTG
Cuts at: 0 96 562 759 1038 1077 1248 1360 1480
Size: 96 466 197 279 39 171 112 120
Cuts at: 1480 2025
Size: 545
Fragments arranged by size:
545 466 279 197 171 120 112 96
39
ApaLI G'TGCA_C
Cuts at: 0 1263 2025
Size: 1263 762
ApoI r'AATT_y
Cuts at: 0 325 802 1000 2025
Size: 325 477 198 1025
Fragments arranged by size:
1025 477 325 198
AvaI C'yCGr_G
Cuts at: 0 937 1583 2025
Size: 937 646 442
Fragments arranged by size:
937 646 442
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1681 1714 2025
Size: 1681 33 311
Fragments arranged by size:
1681 311 33
BanI G'GyrC_C
Cuts at: 0 456 521 2025
Size: 456 65 1504
Fragments arranged by size:
1504 456 65
BanII G_rGCy'C
Cuts at: 0 113 1217 1727 1778 1989 1996 2025
Size: 113 1104 510 51 211 7 29
Fragments arranged by size:
1104 510 211 113 51 29 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 742 800 2025
Size: 742 58 1225
Fragments arranged by size:
1225 742 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 928 962 1178 1212 2025
Size: 928 34 216 34 813
Fragments arranged by size:
928 813 216 34 34
BclI T'GATC_A
Cuts at: 0 1079 2025
Size: 1079 946
BglII A'GATC_T
Cuts at: 0 198 2025
Size: 198 1827
BlpI GC'TnA_GC
Cuts at: 0 748 1211 2025
Size: 748 463 814
Fragments arranged by size:
814 748 463
Bme1580I G_kGCm'C
Cuts at: 0 1267 1796 2025
Size: 1267 529 229
Fragments arranged by size:
1267 529 229
BmgBI CAC'GTC
Cuts at: 0 615 1350 2025
Size: 615 735 675
Fragments arranged by size:
735 675 615
BmrI ACTGGGnnnn_n'
Cuts at: 0 1399 1568 1706 1990 2025
Size: 1399 169 138 284 35
Fragments arranged by size:
1399 284 169 138 35
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 45 294 2025
Size: 45 249 1731
Fragments arranged by size:
1731 249 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 130 163 568 592 1156 1264 1915
Size: 15 115 33 405 24 564 108 651
Cuts at: 1915 2025
Size: 110
Fragments arranged by size:
651 564 405 115 110 108 33 24
15
Bpu10I CC'TnA_GC
Cuts at: 0 1504 2025
Size: 1504 521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 2025
Size: 1006 1019
BsaI GGTCTCn'nnnn_
Cuts at: 0 132 968 1420 2025
Size: 132 836 452 605
Fragments arranged by size:
836 605 452 132
BsaAI yAC'GTr
Cuts at: 0 401 2025
Size: 401 1624
BsaWI w'CCGG_w
Cuts at: 0 45 404 1770 2025
Size: 45 359 1366 255
Fragments arranged by size:
1366 359 255 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 712 742 1451 1481 2025
Size: 712 30 709 30 544
Fragments arranged by size:
712 709 544 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 1177 1735 2025
Size: 349 828 558 290
Fragments arranged by size:
828 558 349 290
BseYI C'CCAG_C
Cuts at: 0 1248 1331 1681 1777 1782 2025
Size: 1248 83 350 96 5 243
Fragments arranged by size:
1248 350 243 96 83 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 973 1062 1462 1513 1736 2025
Size: 319 654 89 400 51 223 289
Fragments arranged by size:
654 400 319 289 223 89 51
BsiEI CG_ry'CG
Cuts at: 0 1638 2025
Size: 1638 387
BsiHKAI G_wGCw'C
Cuts at: 0 113 1217 1267 1727 2025
Size: 113 1104 50 460 298
Fragments arranged by size:
1104 460 298 113 50
Bsp1286I G_dGCh'C
Cuts at: 0 113 1217 1267 1727 1778 1796 1989 1996
Size: 113 1104 50 460 51 18 193 7
Cuts at: 1996 2025
Size: 29
Fragments arranged by size:
1104 460 193 113 51 50 29 18
7
BspEI T'CCGG_A
Cuts at: 0 45 2025
Size: 45 1980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1499 2025
Size: 1499 526
BsrBI CCG'CTC
Cuts at: 0 699 1116 2025
Size: 699 417 909
Fragments arranged by size:
909 699 417
BsrFI r'CCGG_y
Cuts at: 0 1677 2025
Size: 1677 348
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1305 2025
Size: 1305 720
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2003 2025
Size: 2003 22
BstYI r'GATC_y
Cuts at: 0 198 469 2025
Size: 198 271 1556
Fragments arranged by size:
1556 271 198
BtsI GCAGTG_nn'
Cuts at: 0 526 956 1152 1249 2025
Size: 526 430 196 97 776
Fragments arranged by size:
776 526 430 196 97
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1533 1568 2025
Size: 1533 35 457
Fragments arranged by size:
1533 457 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 124 2025
Size: 124 1901
EaeI y'GGCC_r
Cuts at: 0 2 1049 1598 1635 1841 1979 2025
Size: 2 1047 549 37 206 138 46
Fragments arranged by size:
1047 549 206 138 46 37 2
EagI C'GGCC_G
Cuts at: 0 1635 2025
Size: 1635 390
EarI CTCTTCn'nnn_
Cuts at: 0 281 812 1118 2025
Size: 281 531 306 907
Fragments arranged by size:
907 531 306 281
EcoICRI GAG'CTC
Cuts at: 0 111 1215 1725 2025
Size: 111 1104 510 300
Fragments arranged by size:
1104 510 300 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 130 163 458 568 571 592 781
Size: 15 115 33 295 110 3 21 189
Cuts at: 781 877 891 1156 1264 1350 1915 2025
Size: 96 14 265 108 86 565 110
Fragments arranged by size:
565 295 265 189 115 110 110 108
96 86 33 21 15 14 3
EcoO109I rG'GnC_Cy
Cuts at: 0 62 943 1287 2025
Size: 62 881 344 738
Fragments arranged by size:
881 738 344 62
EcoRI G'AATT_C
Cuts at: 0 325 2025
Size: 325 1700
EcoRV GAT'ATC
Cuts at: 0 231 2025
Size: 231 1794
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1078 2025
Size: 1078 947
FspI TGC'GCA
Cuts at: 0 646 2025
Size: 646 1379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 61 93 1548 1566 1580 1598
Size: 10 32 19 32 1455 18 14 18
Cuts at: 1598 2025
Size: 427
Fragments arranged by size:
1455 427 32 32 19 18 18 14
10
KpnI G_GTAC'C
Cuts at: 0 525 2025
Size: 525 1500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 2025
Size: 320 1705
MscI TGG'CCA
Cuts at: 0 4 1051 1600 1843 1981 2025
Size: 4 1047 549 243 138 44
Fragments arranged by size:
1047 549 243 138 44 4
MslI CAynn'nnrTG
Cuts at: 0 238 1270 2025
Size: 238 1032 755
Fragments arranged by size:
1032 755 238
MspA1I CmG'CkG
Cuts at: 0 426 684 756 1233 1335 1634 1640 1695
Size: 426 258 72 477 102 299 6 55
Cuts at: 1695 2025
Size: 330
Fragments arranged by size:
477 426 330 299 258 102 72 55
6
NaeI GCC'GGC
Cuts at: 0 1679 2025
Size: 1679 346
NgoMIV G'CCGG_C
Cuts at: 0 1677 2025
Size: 1677 348
NotI GC'GGCC_GC
Cuts at: 0 1635 2025
Size: 1635 390
NspI r_CATG'y
Cuts at: 0 144 2025
Size: 144 1881
PasI CC'CwG_GG
Cuts at: 0 1519 2025
Size: 1519 506
PflMI CCAn_nnn'nTGG
Cuts at: 0 1596 2025
Size: 1596 429
PfoI T'CCnGG_A
Cuts at: 0 104 545 608 692 1892 2025
Size: 104 441 63 84 1200 133
Fragments arranged by size:
1200 441 133 104 84 63
PpuMI rG'GwC_Cy
Cuts at: 0 1287 2025
Size: 1287 738
PstI C_TGCA'G
Cuts at: 0 269 425 509 539 893 951 1310 1871
Size: 269 156 84 30 354 58 359 561
Cuts at: 1871 2025
Size: 154
Fragments arranged by size:
561 359 354 269 156 154 84 58
30
PvuII CAG'CTG
Cuts at: 0 684 756 1233 1335 1695 2025
Size: 684 72 477 102 360 330
Fragments arranged by size:
684 477 360 330 102 72
SacI G_AGCT'C
Cuts at: 0 113 1217 1727 2025
Size: 113 1104 510 298
Fragments arranged by size:
1104 510 298 113
SbfI CC_TGCA'GG
Cuts at: 0 1871 2025
Size: 1871 154
SexAI A'CCwGG_T
Cuts at: 0 517 1532 1814 2025
Size: 517 1015 282 211
Fragments arranged by size:
1015 517 282 211
SfcI C'TryA_G
Cuts at: 0 265 421 505 535 889 947 1306 1690
Size: 265 156 84 30 354 58 359 384
Cuts at: 1690 1867 2025
Size: 177 158
Fragments arranged by size:
384 359 354 265 177 158 156 84
58 30
SmlI C'TyrA_G
Cuts at: 0 985 2025
Size: 985 1040
StuI AGG'CCT
Cuts at: 0 382 479 991 1022 2025
Size: 382 97 512 31 1003
Fragments arranged by size:
1003 512 382 97 31
StyI C'CwwG_G
Cuts at: 0 281 671 2025
Size: 281 390 1354
Fragments arranged by size:
1354 390 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 822 2025
Size: 822 1203
TatI w'GTAC_w
Cuts at: 0 635 665 2025
Size: 635 30 1360
Fragments arranged by size:
1360 635 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 712 744 1451 1483 2025
Size: 712 32 707 32 542
Fragments arranged by size:
712 707 542 32 32
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AloI AlwNI ApaLI
ApoI AvaI BaeI BanI BanII BbsI BcgI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI
BstXI BstYI BtsI CspCI DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI Hin4I
KpnI MmeI MscI MslI MspA1I NaeI NgoMIV NotI
NspI PasI PflMI PfoI PpuMI PstI PvuII SacI
SbfI SexAI SfcI SmlI StuI StyI TaqII TatI
TstI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
ApaI AscI AseI AsiSI AvrII BamHI BbeI BbvCI
BciVI BfrBI BglI BmtI BsaBI BsaHI BsiWI BsmI
BsmBI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII EciI
EcoNI FseI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI NarI NcoI NdeI NheI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI