Restriction map for
pF1KE4235
(Linear) (Six-base) MAPSORT of: KIEE4235.seq Check: 2844 from: 1 to: 2574
KIEE4235.orf
With 182 enzymes: SgfI *
October 29, 2018 20:57 ..
AatII G_ACGT'C
Cuts at: 0 2522 2574
Size: 2522 52
AclI AA'CG_TT
Cuts at: 0 1574 2574
Size: 1574 1000
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 2574
Size: 327 2247
AflII C'TTAA_G
Cuts at: 0 922 2424 2574
Size: 922 1502 150
Fragments arranged by size:
1502 922 150
AflIII A'CryG_T
Cuts at: 0 494 1592 2574
Size: 494 1098 982
Fragments arranged by size:
1098 982 494
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2494 2526 2574
Size: 2494 32 48
Fragments arranged by size:
2494 48 32
AlwNI CAG_nnn'CTG
Cuts at: 0 957 1569 2574
Size: 957 612 1005
Fragments arranged by size:
1005 957 612
ApaI G_GGCC'C
Cuts at: 0 781 2574
Size: 781 1793
ApoI r'AATT_y
Cuts at: 0 908 2574
Size: 908 1666
AvaI C'yCGr_G
Cuts at: 0 52 208 264 2574
Size: 52 156 56 2310
Fragments arranged by size:
2310 156 56 52
BamHI G'GATC_C
Cuts at: 0 1528 2574
Size: 1528 1046
BanI G'GyrC_C
Cuts at: 0 268 1891 2574
Size: 268 1623 683
Fragments arranged by size:
1623 683 268
BanII G_rGCy'C
Cuts at: 0 59 356 443 781 1887 1969 2161 2547
Size: 59 297 87 338 1106 82 192 386
Cuts at: 2547 2574
Size: 27
Fragments arranged by size:
1106 386 338 297 192 87 82 59
27
BbeI G_GCGC'C
Cuts at: 0 272 2574
Size: 272 2302
BbsI GAAGACnn'nnnn_
Cuts at: 0 1038 1544 1595 2574
Size: 1038 506 51 979
Fragments arranged by size:
1038 979 506 51
BbvCI CC'TCA_GC
Cuts at: 0 736 1562 2574
Size: 736 826 1012
Fragments arranged by size:
1012 826 736
BciVI GTATCCnnnnn_n'
Cuts at: 0 1347 1702 2574
Size: 1347 355 872
Fragments arranged by size:
1347 872 355
BglI GCCn_nnn'nGGC
Cuts at: 0 266 2574
Size: 266 2308
BglII A'GATC_T
Cuts at: 0 377 890 2574
Size: 377 513 1684
Fragments arranged by size:
1684 513 377
BlpI GC'TnA_GC
Cuts at: 0 337 2155 2574
Size: 337 1818 419
Fragments arranged by size:
1818 419 337
Bme1580I G_kGCm'C
Cuts at: 0 781 2574
Size: 781 1793
BmrI ACTGGGnnnn_n'
Cuts at: 0 311 372 1856 2036 2468 2574
Size: 311 61 1484 180 432 106
Fragments arranged by size:
1484 432 311 180 106 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 556 934 1020 1327 2143 2298 2574
Size: 406 150 378 86 307 816 155 276
Fragments arranged by size:
816 406 378 307 276 155 150 86
Bpu10I CC'TnA_GC
Cuts at: 0 635 736 1562 2574
Size: 635 101 826 1012
Fragments arranged by size:
1012 826 635 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1482 2518 2574
Size: 427 1055 1036 56
Fragments arranged by size:
1055 1036 427 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 451 2213 2574
Size: 451 1762 361
Fragments arranged by size:
1762 451 361
BsaAI yAC'GTr
Cuts at: 0 497 693 2574
Size: 497 196 1881
Fragments arranged by size:
1881 497 196
BsaHI Gr'CG_yC
Cuts at: 0 269 2519 2574
Size: 269 2250 55
Fragments arranged by size:
2250 269 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 313 343 688 718 1193 1223 2331 2361
Size: 313 30 345 30 475 30 1108 30
Cuts at: 2361 2494 2524 2574
Size: 133 30 50
Fragments arranged by size:
1108 475 345 313 133 50 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 451 496 738 990 2041 2555 2574
Size: 337 114 45 242 252 1051 514 19
Fragments arranged by size:
1051 514 337 252 242 114 45 19
BseYI C'CCAG_C
Cuts at: 0 168 680 1092 1696 1837 1924 2574
Size: 168 512 412 604 141 87 650
Fragments arranged by size:
650 604 512 412 168 141 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 2574
Size: 295 2279
BsiHKAI G_wGCw'C
Cuts at: 0 88 356 443 1887 2013 2344 2574
Size: 88 268 87 1444 126 331 230
Fragments arranged by size:
1444 331 268 230 126 88 87
Bsp1286I G_dGCh'C
Cuts at: 0 59 88 356 443 781 1887 1969 2013
Size: 59 29 268 87 338 1106 82 44
Cuts at: 2013 2161 2344 2547 2574
Size: 148 183 203 27
Fragments arranged by size:
1106 338 268 203 183 148 87 82
59 44 29 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 740 2574
Size: 740 1834
BsrBI CCG'CTC
Cuts at: 0 32 471 2574
Size: 32 439 2103
Fragments arranged by size:
2103 439 32
BsrDI GCAATG_nn'
Cuts at: 0 608 1193 2574
Size: 608 585 1381
Fragments arranged by size:
1381 608 585
BsrFI r'CCGG_y
Cuts at: 0 3 2574
Size: 3 2571
BsrGI T'GTAC_A
Cuts at: 0 1595 2574
Size: 1595 979
BssHII G'CGCG_C
Cuts at: 0 332 2574
Size: 332 2242
BssSI C'ACGA_G
Cuts at: 0 124 2558 2574
Size: 124 2434 16
Fragments arranged by size:
2434 124 16
BstAPI GCAn_nnn'nTGC
Cuts at: 0 83 2574
Size: 83 2491
BstEII G'GTnAC_C
Cuts at: 0 2035 2574
Size: 2035 539
BstXI CCAn_nnnn'nTGG
Cuts at: 0 943 2139 2574
Size: 943 1196 435
Fragments arranged by size:
1196 943 435
BstYI r'GATC_y
Cuts at: 0 377 566 890 1528 2574
Size: 377 189 324 638 1046
Fragments arranged by size:
1046 638 377 324 189
Bsu36I CC'TnA_GG
Cuts at: 0 1796 2574
Size: 1796 778
BtgI C'CryG_G
Cuts at: 0 59 722 1016 2547 2574
Size: 59 663 294 1531 27
Fragments arranged by size:
1531 663 294 59 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 233 693 2574
Size: 233 460 1881
Fragments arranged by size:
1881 460 233
EaeI y'GGCC_r
Cuts at: 0 660 803 1607 2574
Size: 660 143 804 967
Fragments arranged by size:
967 804 660 143
EarI CTCTTCn'nnn_
Cuts at: 0 505 1012 1223 1264 2574
Size: 505 507 211 41 1310
Fragments arranged by size:
1310 507 505 211 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 865 2574
Size: 88 777 1709
Fragments arranged by size:
1709 777 88
EcoICRI GAG'CTC
Cuts at: 0 354 441 1885 2574
Size: 354 87 1444 689
Fragments arranged by size:
1444 689 354 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 406 556 934 1020 1327 2143 2298
Size: 327 79 150 378 86 307 816 155
Cuts at: 2298 2574
Size: 276
Fragments arranged by size:
816 378 327 307 276 155 150 86
79
EcoO109I rG'GnC_Cy
Cuts at: 0 528 777 2470 2574
Size: 528 249 1693 104
Fragments arranged by size:
1693 528 249 104
EcoRI G'AATT_C
Cuts at: 0 908 2574
Size: 908 1666
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2417 2574
Size: 2417 157
FspI TGC'GCA
Cuts at: 0 73 504 2574
Size: 73 431 2070
Fragments arranged by size:
2070 431 73
HaeII r_GCGC'y
Cuts at: 0 272 2574
Size: 272 2302
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2282 2314 2331 2363 2574
Size: 2282 32 17 32 211
Fragments arranged by size:
2282 211 32 32 17
HincII GTy'rAC
Cuts at: 0 2022 2574
Size: 2022 552
HindIII A'AGCT_T
Cuts at: 0 925 2574
Size: 925 1649
KasI G'GCGC_C
Cuts at: 0 268 2574
Size: 268 2306
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1565 2574
Size: 869 696 1009
Fragments arranged by size:
1009 869 696
MscI TGG'CCA
Cuts at: 0 805 1609 2574
Size: 805 804 965
Fragments arranged by size:
965 805 804
MslI CAynn'nnrTG
Cuts at: 0 1120 1345 1375 1741 2574
Size: 1120 225 30 366 833
Fragments arranged by size:
1120 833 366 225 30
MspA1I CmG'CkG
Cuts at: 0 684 2295 2574
Size: 684 1611 279
Fragments arranged by size:
1611 684 279
NaeI GCC'GGC
Cuts at: 0 5 2574
Size: 5 2569
NarI GG'CG_CC
Cuts at: 0 269 2574
Size: 269 2305
NcoI C'CATG_G
Cuts at: 0 59 722 1016 2547 2574
Size: 59 663 294 1531 27
Fragments arranged by size:
1531 663 294 59 27
NgoMIV G'CCGG_C
Cuts at: 0 3 2574
Size: 3 2571
NspI r_CATG'y
Cuts at: 0 1596 1746 2574
Size: 1596 150 828
Fragments arranged by size:
1596 828 150
PasI CC'CwG_GG
Cuts at: 0 2335 2574
Size: 2335 239
PciI A'CATG_T
Cuts at: 0 1592 2574
Size: 1592 982
PflMI CCAn_nnn'nTGG
Cuts at: 0 761 957 2574
Size: 761 196 1617
Fragments arranged by size:
1617 761 196
PmlI CAC'GTG
Cuts at: 0 497 693 2574
Size: 497 196 1881
Fragments arranged by size:
1881 497 196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2494 2526 2574
Size: 2494 32 48
Fragments arranged by size:
2494 48 32
PpuMI rG'GwC_Cy
Cuts at: 0 528 2574
Size: 528 2046
PshAI GACnn'nnGTC
Cuts at: 0 2042 2574
Size: 2042 532
PspOMI G'GGCC_C
Cuts at: 0 777 2574
Size: 777 1797
PspXI vC'TCGA_Gb
Cuts at: 0 208 2574
Size: 208 2366
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2425 2457 2574
Size: 2425 32 117
Fragments arranged by size:
2425 117 32
PvuII CAG'CTG
Cuts at: 0 684 2295 2574
Size: 684 1611 279
Fragments arranged by size:
1611 684 279
SacI G_AGCT'C
Cuts at: 0 356 443 1887 2574
Size: 356 87 1444 687
Fragments arranged by size:
1444 687 356 87
SapI GCTCTTCn'nnn_
Cuts at: 0 1223 2574
Size: 1223 1351
ScaI AGT'ACT
Cuts at: 0 247 2574
Size: 247 2327
SfcI C'TryA_G
Cuts at: 0 225 640 2301 2574
Size: 225 415 1661 273
Fragments arranged by size:
1661 415 273 225
SfoI GGC'GCC
Cuts at: 0 270 2574
Size: 270 2304
SmaI CCC'GGG
Cuts at: 0 266 2574
Size: 266 2308
SmlI C'TyrA_G
Cuts at: 0 208 442 922 1497 2424 2497 2574
Size: 208 234 480 575 927 73 77
Fragments arranged by size:
927 575 480 234 208 77 73
StyI C'CwwG_G
Cuts at: 0 59 722 1016 1517 1640 2547 2574
Size: 59 663 294 501 123 907 27
Fragments arranged by size:
907 663 501 294 123 59 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1879 2574
Size: 1879 695
TatI w'GTAC_w
Cuts at: 0 245 1431 1595 2261 2574
Size: 245 1186 164 666 313
Fragments arranged by size:
1186 666 313 245 164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 313 345 688 720 1193 1225 2574
Size: 313 32 343 32 473 32 1349
Fragments arranged by size:
1349 473 343 313 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1482 2574
Size: 1482 1092
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 552 798 1100 1161 1871 2574
Size: 552 246 302 61 710 703
Fragments arranged by size:
710 703 552 302 246 61
XhoI C'TCGA_G
Cuts at: 0 208 2574
Size: 208 2366
XmaI C'CCGG_G
Cuts at: 0 264 2574
Size: 264 2310
XmnI GAAnn'nnTTC
Cuts at: 0 769 2574
Size: 769 1805
ZraI GAC'GTC
Cuts at: 0 2520 2574
Size: 2520 54
Enzymes that do cut:
AatII AclI AcuI AflII AflIII AloI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuII SacI SapI ScaI SfcI SfoI SmaI
SmlI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AgeI AhdI AleI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BcgI BclI
BfrBI BmgBI BmtI BplI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BstBI BstZ17I BtsI ClaI
CspCI DraI DraIII DrdI EagI EcoNI EcoRV FseI
FspAI HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PfoI PmeI PsiI PstI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI