Restriction map for
pF1KE4265
(Linear) (Six-base) MAPSORT of: KIEE4265.seq Check: 1746 from: 1 to: 3186
KIEE4265.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
Acc65I G'GTAC_C
Cuts at: 0 1073 1826 3186
Size: 1073 753 1360
Fragments arranged by size:
1360 1073 753
AclI AA'CG_TT
Cuts at: 0 2972 3186
Size: 2972 214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1160 1519 1690 3100 3186
Size: 1160 359 171 1410 86
Fragments arranged by size:
1410 1160 359 171 86
AflIII A'CryG_T
Cuts at: 0 1962 3186
Size: 1962 1224
AhdI GACnn_n'nnGTC
Cuts at: 0 2444 2583 3186
Size: 2444 139 603
Fragments arranged by size:
2444 603 139
AleI CACnn'nnGTG
Cuts at: 0 1013 2144 3186
Size: 1013 1131 1042
Fragments arranged by size:
1131 1042 1013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2990 3186
Size: 2990 196
AlwNI CAG_nnn'CTG
Cuts at: 0 144 276 1228 1660 3186
Size: 144 132 952 432 1526
Fragments arranged by size:
1526 952 432 144 132
ApoI r'AATT_y
Cuts at: 0 68 1593 3186
Size: 68 1525 1593
Fragments arranged by size:
1593 1525 68
AvaI C'yCGr_G
Cuts at: 0 9 2609 3186
Size: 9 2600 577
Fragments arranged by size:
2600 577 9
AvrII C'CTAG_G
Cuts at: 0 2580 3186
Size: 2580 606
BanI G'GyrC_C
Cuts at: 0 1073 1826 2230 2304 3057 3186
Size: 1073 753 404 74 753 129
Fragments arranged by size:
1073 753 753 404 129 74
BanII G_rGCy'C
Cuts at: 0 78 840 932 1064 2138 2989 3019 3186
Size: 78 762 92 132 1074 851 30 167
Fragments arranged by size:
1074 851 762 167 132 92 78 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 591 1525 2411 3186
Size: 591 934 886 775
Fragments arranged by size:
934 886 775 591
BciVI GTATCCnnnnn_n'
Cuts at: 0 2895 3186
Size: 2895 291
BglII A'GATC_T
Cuts at: 0 583 3186
Size: 583 2603
Bme1580I G_kGCm'C
Cuts at: 0 1041 3186
Size: 1041 2145
BmgBI CAC'GTC
Cuts at: 0 2747 3186
Size: 2747 439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 124 269 864 2081 2483 2878 2929
Size: 121 3 145 595 1217 402 395 51
Cuts at: 2929 3006 3071 3186
Size: 77 65 115
Fragments arranged by size:
1217 595 402 395 145 121 115 77
65 51 3
Bpu10I CC'TnA_GC
Cuts at: 0 1069 2651 2807 3186
Size: 1069 1582 156 379
Fragments arranged by size:
1582 1069 379 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 2695 3186
Size: 369 2326 491
Fragments arranged by size:
2326 491 369
BsaHI Gr'CG_yC
Cuts at: 0 2508 3186
Size: 2508 678
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 573 603 2416 2446 3186
Size: 573 30 1813 30 740
Fragments arranged by size:
1813 740 573 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 631 1777 2498 2559 3009 3186
Size: 631 1146 721 61 450 177
Fragments arranged by size:
1146 721 631 450 177 61
BseYI C'CCAG_C
Cuts at: 0 1040 1267 3186
Size: 1040 227 1919
Fragments arranged by size:
1919 1040 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 2347 3094 3186
Size: 551 1796 747 92
Fragments arranged by size:
1796 747 551 92
BsiHKAI G_wGCw'C
Cuts at: 0 78 1064 2096 2872 2989 3019 3186
Size: 78 986 1032 776 117 30 167
Fragments arranged by size:
1032 986 776 167 117 78 30
BsmI GAATG_Cn'
Cuts at: 0 439 915 1204 3186
Size: 439 476 289 1982
Fragments arranged by size:
1982 476 439 289
BsmBI CGTCTCn'nnnn_
Cuts at: 0 172 3186
Size: 172 3014
Bsp1286I G_dGCh'C
Cuts at: 0 78 840 932 1041 1064 2096 2138 2872
Size: 78 762 92 109 23 1032 42 734
Cuts at: 2872 2989 3019 3186
Size: 117 30 167
Fragments arranged by size:
1032 762 734 167 117 109 92 78
42 30 23
BspHI T'CATG_A
Cuts at: 0 450 777 1319 3186
Size: 450 327 542 1867
Fragments arranged by size:
1867 542 450 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3114 3150 3186
Size: 3114 36 36
Fragments arranged by size:
3114 36 36
BsrBI CCG'CTC
Cuts at: 0 2541 2655 3186
Size: 2541 114 531
Fragments arranged by size:
2541 531 114
BsrDI GCAATG_nn'
Cuts at: 0 2532 3186
Size: 2532 654
BsrFI r'CCGG_y
Cuts at: 0 2696 3186
Size: 2696 490
BssSI C'ACGA_G
Cuts at: 0 3012 3186
Size: 3012 174
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2881 3186
Size: 2881 305
BstEII G'GTnAC_C
Cuts at: 0 1170 3186
Size: 1170 2016
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2125 3186
Size: 2125 1061
BstYI r'GATC_y
Cuts at: 0 141 583 3186
Size: 141 442 2603
Fragments arranged by size:
2603 442 141
Bsu36I CC'TnA_GG
Cuts at: 0 1709 2980 3094 3186
Size: 1709 1271 114 92
Fragments arranged by size:
1709 1271 114 92
BtgI C'CryG_G
Cuts at: 0 1683 2628 2673 2945 3186
Size: 1683 945 45 272 241
Fragments arranged by size:
1683 945 272 241 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1257 1880 3186
Size: 1257 623 1306
Fragments arranged by size:
1306 1257 623
BtsI GCAGTG_nn'
Cuts at: 0 2394 3186
Size: 2394 792
DraI TTT'AAA
Cuts at: 0 420 3186
Size: 420 2766
EaeI y'GGCC_r
Cuts at: 0 1895 2529 2670 3186
Size: 1895 634 141 516
Fragments arranged by size:
1895 634 516 141
EarI CTCTTCn'nnn_
Cuts at: 0 1115 1278 3186
Size: 1115 163 1908
Fragments arranged by size:
1908 1115 163
EcoICRI GAG'CTC
Cuts at: 0 76 1062 2987 3017 3186
Size: 76 986 1925 30 169
Fragments arranged by size:
1925 986 169 76 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 124 269 864 1160 1519 1690 2081
Size: 121 3 145 595 296 359 171 391
Cuts at: 2081 2483 2878 2929 3006 3071 3100 3186
Size: 402 395 51 77 65 29 86
Fragments arranged by size:
595 402 395 391 359 296 171 145
121 86 77 65 51 29 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3134 3186
Size: 3134 52
EcoO109I rG'GnC_Cy
Cuts at: 0 1394 2585 2613 3139 3186
Size: 1394 1191 28 526 47
Fragments arranged by size:
1394 1191 526 47 28
HaeII r_GCGC'y
Cuts at: 0 1771 3186
Size: 1771 1415
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 988 1020 1636 1668 1933 1965 2398 2416
Size: 988 32 616 32 265 32 433 18
Cuts at: 2416 2430 2448 2462 2569 2601 2790 2822 3186
Size: 14 18 14 107 32 189 32 364
Fragments arranged by size:
988 616 433 364 265 189 107 32
32 32 32 32 18 18 14 14
HincII GTy'rAC
Cuts at: 0 2577 3186
Size: 2577 609
KpnI G_GTAC'C
Cuts at: 0 1077 1830 3186
Size: 1077 753 1356
Fragments arranged by size:
1356 1077 753
MfeI C'AATT_G
Cuts at: 0 2801 3186
Size: 2801 385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 3186
Size: 468 2718
MscI TGG'CCA
Cuts at: 0 1897 2531 2672 3186
Size: 1897 634 141 514
Fragments arranged by size:
1897 634 514 141
MslI CAynn'nnrTG
Cuts at: 0 958 1013 1967 2144 3186
Size: 958 55 954 177 1042
Fragments arranged by size:
1042 958 954 177 55
MspA1I CmG'CkG
Cuts at: 0 3078 3186
Size: 3078 108
NaeI GCC'GGC
Cuts at: 0 2698 3186
Size: 2698 488
NcoI C'CATG_G
Cuts at: 0 2673 3186
Size: 2673 513
NdeI CA'TA_TG
Cuts at: 0 506 3186
Size: 506 2680
NgoMIV G'CCGG_C
Cuts at: 0 2696 3186
Size: 2696 490
NspI r_CATG'y
Cuts at: 0 1966 2557 2884 3186
Size: 1966 591 327 302
Fragments arranged by size:
1966 591 327 302
PasI CC'CwG_GG
Cuts at: 0 1398 3156 3186
Size: 1398 1758 30
Fragments arranged by size:
1758 1398 30
PciI A'CATG_T
Cuts at: 0 1962 3186
Size: 1962 1224
PfoI T'CCnGG_A
Cuts at: 0 137 190 3186
Size: 137 53 2996
Fragments arranged by size:
2996 137 53
PpuMI rG'GwC_Cy
Cuts at: 0 1394 2585 3139 3186
Size: 1394 1191 554 47
Fragments arranged by size:
1394 1191 554 47
PsiI TTA'TAA
Cuts at: 0 1889 3186
Size: 1889 1297
PstI C_TGCA'G
Cuts at: 0 1391 2624 3186
Size: 1391 1233 562
Fragments arranged by size:
1391 1233 562
PvuII CAG'CTG
Cuts at: 0 3078 3186
Size: 3078 108
SacI G_AGCT'C
Cuts at: 0 78 1064 2989 3019 3186
Size: 78 986 1925 30 167
Fragments arranged by size:
1925 986 167 78 30
SanDI GG'GwC_CC
Cuts at: 0 2585 3186
Size: 2585 601
SapI GCTCTTCn'nnn_
Cuts at: 0 1278 3186
Size: 1278 1908
ScaI AGT'ACT
Cuts at: 0 769 3039 3186
Size: 769 2270 147
Fragments arranged by size:
2270 769 147
SfcI C'TryA_G
Cuts at: 0 1387 2620 3186
Size: 1387 1233 566
Fragments arranged by size:
1387 1233 566
SmaI CCC'GGG
Cuts at: 0 2611 3186
Size: 2611 575
SmlI C'TyrA_G
Cuts at: 0 348 2710 3186
Size: 348 2362 476
Fragments arranged by size:
2362 476 348
SphI G_CATG'C
Cuts at: 0 2884 3186
Size: 2884 302
SspI AAT'ATT
Cuts at: 0 187 685 1336 3186
Size: 187 498 651 1850
Fragments arranged by size:
1850 651 498 187
StuI AGG'CCT
Cuts at: 0 2667 3186
Size: 2667 519
StyI C'CwwG_G
Cuts at: 0 2420 2580 2673 3186
Size: 2420 160 93 513
Fragments arranged by size:
2420 513 160 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1003 1797 3075 3186
Size: 1003 794 1278 111
Fragments arranged by size:
1278 1003 794 111
TatI w'GTAC_w
Cuts at: 0 767 863 1859 3037 3186
Size: 767 96 996 1178 149
Fragments arranged by size:
1178 996 767 149 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 573 605 1201 1233 3186
Size: 573 32 596 32 1953
Fragments arranged by size:
1953 596 573 32 32
XbaI T'CTAG_A
Cuts at: 0 198 3186
Size: 198 2988
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 293 3186
Size: 293 2893
XmaI C'CCGG_G
Cuts at: 0 2609 3186
Size: 2609 577
Enzymes that do cut:
Acc65I AclI AcuI AflIII AhdI AleI AlfI AlwNI
ApoI AvaI AvrII BanI BanII BbsI BciVI BglII
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI MfeI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NspI PasI PciI PfoI PpuMI PsiI PstI PvuII
SacI SanDI SapI ScaI SfcI SmaI SmlI SphI
SspI StuI StyI TaqII TatI TstI XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AgeI AloI ApaI
ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI
BcgI BclI BfrBI BglI BlpI BmrI BmtI BplI
BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BsrGI
BssHII BstBI BstZ17I ClaI CspCI DraIII DrdI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI MluI NarI NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI Tth111I
XhoI XmnI ZraI