Restriction map for
pF1KE4367
(Linear) (Six-base) MAPSORT of: KIEE4367.seq Check: 6384 from: 1 to: 1158
KIEE4367.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 989 1158
Size: 989 169
AfeI AGC'GCT
Cuts at: 0 39 1158
Size: 39 1119
AflII C'TTAA_G
Cuts at: 0 112 1158
Size: 112 1046
AflIII A'CryG_T
Cuts at: 0 469 756 1158
Size: 469 287 402
Fragments arranged by size:
469 402 287
AhdI GACnn_n'nnGTC
Cuts at: 0 19 1158
Size: 19 1139
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 468 500 1158
Size: 468 32 658
Fragments arranged by size:
658 468 32
AlwNI CAG_nnn'CTG
Cuts at: 0 897 948 967 1141 1158
Size: 897 51 19 174 17
Fragments arranged by size:
897 174 51 19 17
ApaLI G'TGCA_C
Cuts at: 0 294 760 1158
Size: 294 466 398
Fragments arranged by size:
466 398 294
ApoI r'AATT_y
Cuts at: 0 703 1158
Size: 703 455
AvaI C'yCGr_G
Cuts at: 0 33 41 1158
Size: 33 8 1117
Fragments arranged by size:
1117 33 8
BanII G_rGCy'C
Cuts at: 0 1105 1158
Size: 1105 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 117 1158
Size: 117 1041
Bme1580I G_kGCm'C
Cuts at: 0 298 764 1158
Size: 298 466 394
Fragments arranged by size:
466 394 298
BmgBI CAC'GTC
Cuts at: 0 470 1158
Size: 470 688
BmrI ACTGGGnnnn_n'
Cuts at: 0 575 1158
Size: 575 583
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1155 1158
Size: 1155 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 1158
Size: 299 859
Bpu10I CC'TnA_GC
Cuts at: 0 732 1158
Size: 732 426
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 1158
Size: 217 941
BsaI GGTCTCn'nnnn_
Cuts at: 0 826 873 1158
Size: 826 47 285
Fragments arranged by size:
826 285 47
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 77 107 229 259 1000 1030 1158
Size: 77 30 122 30 741 30 128
Fragments arranged by size:
741 128 122 77 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 253 256 601 758 949 996 999 1158
Size: 253 3 345 157 191 47 3 159
Fragments arranged by size:
345 253 191 159 157 47 3 3
BseYI C'CCAG_C
Cuts at: 0 194 576 1158
Size: 194 382 582
Fragments arranged by size:
582 382 194
BsiEI CG_ry'CG
Cuts at: 0 29 1158
Size: 29 1129
BsiHKAI G_wGCw'C
Cuts at: 0 298 764 1158
Size: 298 466 394
Fragments arranged by size:
466 394 298
Bsp1286I G_dGCh'C
Cuts at: 0 298 764 1105 1158
Size: 298 466 341 53
Fragments arranged by size:
466 341 298 53
BsrDI GCAATG_nn'
Cuts at: 0 317 1158
Size: 317 841
BsrFI r'CCGG_y
Cuts at: 0 147 1158
Size: 147 1011
BsrGI T'GTAC_A
Cuts at: 0 352 1158
Size: 352 806
BssSI C'ACGA_G
Cuts at: 0 797 1158
Size: 797 361
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1141 1158
Size: 1141 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 606 1158
Size: 495 111 552
Fragments arranged by size:
552 495 111
BstYI r'GATC_y
Cuts at: 0 107 256 1158
Size: 107 149 902
Fragments arranged by size:
902 149 107
BtgI C'CryG_G
Cuts at: 0 88 1158
Size: 88 1070
DraIII CAC_nnn'GTG
Cuts at: 0 413 1158
Size: 413 745
DrdI GACnn_nn'nnGTC
Cuts at: 0 924 1158
Size: 924 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 826 1158
Size: 826 332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 989 1158
Size: 299 690 169
Fragments arranged by size:
690 299 169
EcoNI CCTnn'n_nnAGG
Cuts at: 0 235 1158
Size: 235 923
EcoO109I rG'GnC_Cy
Cuts at: 0 748 1158
Size: 748 410
HaeII r_GCGC'y
Cuts at: 0 41 54 1158
Size: 41 13 1104
Fragments arranged by size:
1104 41 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 5 37 77 109 433 465 482 514
Size: 5 32 40 32 324 32 17 32
Cuts at: 514 1000 1032 1158
Size: 486 32 126
Fragments arranged by size:
486 324 126 40 32 32 32 32
32 17 5
HincII GTy'rAC
Cuts at: 0 159 1158
Size: 159 999
HindIII A'AGCT_T
Cuts at: 0 432 1158
Size: 432 726
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 676 1158
Size: 350 326 482
Fragments arranged by size:
482 350 326
MslI CAynn'nnrTG
Cuts at: 0 646 1158
Size: 646 512
MspA1I CmG'CkG
Cuts at: 0 553 583 1021 1158
Size: 553 30 438 137
Fragments arranged by size:
553 438 137 30
NcoI C'CATG_G
Cuts at: 0 88 1158
Size: 88 1070
NspI r_CATG'y
Cuts at: 0 1072 1158
Size: 1072 86
PflMI CCAn_nnn'nTGG
Cuts at: 0 89 1098 1158
Size: 89 1009 60
Fragments arranged by size:
1009 89 60
PfoI T'CCnGG_A
Cuts at: 0 57 95 437 1158
Size: 57 38 342 721
Fragments arranged by size:
721 342 57 38
PpuMI rG'GwC_Cy
Cuts at: 0 748 1158
Size: 748 410
PspXI vC'TCGA_Gb
Cuts at: 0 33 41 1158
Size: 33 8 1117
Fragments arranged by size:
1117 33 8
PvuII CAG'CTG
Cuts at: 0 553 1158
Size: 553 605
RsrII CG'GwC_CG
Cuts at: 0 150 1158
Size: 150 1008
SfcI C'TryA_G
Cuts at: 0 920 1158
Size: 920 238
SmlI C'TyrA_G
Cuts at: 0 33 41 112 232 1158
Size: 33 8 71 120 926
Fragments arranged by size:
926 120 71 33 8
StyI C'CwwG_G
Cuts at: 0 88 885 1158
Size: 88 797 273
Fragments arranged by size:
797 273 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 877 1158
Size: 877 281
TatI w'GTAC_w
Cuts at: 0 352 1158
Size: 352 806
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 1158
Size: 229 32 897
Fragments arranged by size:
897 229 32
Tth111I GACn'n_nGTC
Cuts at: 0 72 1158
Size: 72 1086
XhoI C'TCGA_G
Cuts at: 0 33 41 1158
Size: 33 8 1117
Fragments arranged by size:
1117 33 8
XmnI GAAnn'nnTTC
Cuts at: 0 703 1082 1158
Size: 703 379 76
Fragments arranged by size:
703 379 76
Enzymes that do cut:
AcuI AfeI AflII AflIII AhdI AloI AlwNI ApaLI
ApoI AvaI BanII BbsI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BsrDI BsrFI BsrGI BssSI BstAPI BstXI
BstYI BtgI DraIII DrdI EciI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII HindIII MmeI MslI MspA1I NcoI
NspI PflMI PfoI PpuMI PspXI PvuII RsrII SfcI
SmlI StyI TaqII TatI TstI Tth111I XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AleI AlfI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmtI BsaAI BsaBI BsaHI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BssHII BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
EaeI EagI EarI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XcmI XmaI ZraI